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Tuesday, July 29
 

8:29am CDT

GG 1 - Oral Session
Tuesday July 29, 2025 8:29am - 8:30am CDT
Speakers
avatar for Mujahid Hussain

Mujahid Hussain

Graduate Research Assistant, University of Florida
I am pursuing my PhD in the Horticultural Sciences Department, University of Florida in Dr. Kevin Folta's lab. My PhD project is related to exploring molecular basis of low chill apple cultivars with a focus on how genetic and regulatory mechanisms of low chill apple cultivars influence... Read More →
Tuesday July 29, 2025 8:29am - 8:30am CDT
Foster 2

8:30am CDT

GG 1 - Genetic diversity in low chill apple germplasm
Tuesday July 29, 2025 8:30am - 8:45am CDT
There is an urgent need to diversify high-value fruit crops in low-chill areas, especially as climate change decreases the number of chill hours in cropping areas. Apple is largely considered a temperate crop, yet a subset of germplasm exhibits strong flowering responses even after minimal chill. These genotypes originate from Israeli breeding programs, as well as heritage commercial varieties and backyard discoveries. Future breeding efforts will benefit from characterization of the genetic mechanisms that govern flowering in response to limited chill. In this study we hypothesized that examination of sequence polymorphisms and flowering associated gene sequence differences may permit grouping of low chill materials based on common mechanisms. Relatedness was examined using a series of simple sequence repeat markers (SSRs). In addition, genomic sequence from a number of low chill accessions, including Dorsett Golden, Shell of Alabama, and an early-flowering accession from Mississippi, was compared to publicly available reads from ‘Anna’ (low chill), moderate chill (‘Fuji’, ‘Gala’) and high chill (‘Honeycrisp’; ‘Antonovka’) varieties. Genomes were aligned to Golden delicious reference genome, and shared and unique variants were identified. The data show that all low and moderately low chill cultivars share common sequence polymorphisms not found in high-chill germplasm. Examination of flowering and dormancy-related genes shows common sequence polymorphisms shared within low chill materials that contrast against high chill genotypes. These include members of MADS-boxes family, Frigida family, Early bud break (AP2/ERF) family, auxin responsive factors, transcription factors DELLA family, FRIGIDA INTERACTING PROTEIN, and others. This study illuminates potential mechanisms of low-chill responses, opening opportunities for marker-assisted breeding and increased genetic diversity in development of low chill apple cultivars.
Speakers
avatar for Mujahid Hussain

Mujahid Hussain

Graduate Research Assistant, University of Florida
I am pursuing my PhD in the Horticultural Sciences Department, University of Florida in Dr. Kevin Folta's lab. My PhD project is related to exploring molecular basis of low chill apple cultivars with a focus on how genetic and regulatory mechanisms of low chill apple cultivars influence... Read More →
Co-authors
JS

Jugpreet Singh

University of Florida, Tropical Research and Education Center
NA
KF

Kevin Folta

University of florida
NA
Tuesday July 29, 2025 8:30am - 8:45am CDT
Foster 2
  Oral presentation, Genetics and Germplasm 1

8:45am CDT

GG 1 - The Origin Story of Dorsett Golden apple; New Evidence from Horticultural Forensics
Tuesday July 29, 2025 8:45am - 9:00am CDT
Prior to the formal breeding programsof the 19th century, farmers contributed to apple (Malus× domestica Borkh.) breeding by selecting trees based on the desirable characteristics. The transcontinental seed spread was common, and modern breeding programs identified elite commercial trees and high quality fruits. There is a rekindling interest in low-chill apples, both as a high value crop in the USA Southeast, as well as development of new varieties to confront climate change. But the genetic record is poor. Dorsett Golden (DG) is low chill apple cultivar believed to have been discovered in The Bahamas by a Mrs. Dorsett in 1950. As legend has it, she was a jet-setting traveler that loved apples, and planted a set of trees when she relocated to the Bahamas. This allegedly led to the identification of ‘Dorsett Golden’. While this quickly became the accepted story, some questioned the claim. Observations of phenological data in DG trees next to other Israeli germplasm (e.g. ‘Anna’, ‘Ein Shemer’) led Dr. Wayne Sherman to posit in 1980 that DG’s origin was likely from the Israeli breeding program. To test this hypothesis, whole genome sequence from DG was compared to ‘Anna’ and other reference sequences in public databases. Consistent with Dr. Sherman’s 45 year old predictions, DG shares it’s most significant sequence similarity to ‘Anna’, and less with other low-chill varieties. The majority (~92%) of the total variants are in intergenic, upstream, downstream, or intronic regions suggesting recent divergence of ‘Anna’ and DG. The results are consistent with Dr. Sherman’s phenological data that suggest that DG possessed genetics more similar to Israeli genotypes than a chance seedling from Golden Delicious as legend describes. Most importantly, the study illustrates the power of genomic sequencing in selection of parents for low-chill apple crosses as well as debunking horticulutral methology.
Speakers
avatar for Mujahid Hussain

Mujahid Hussain

Graduate Research Assistant, University of Florida
I am pursuing my PhD in the Horticultural Sciences Department, University of Florida in Dr. Kevin Folta's lab. My PhD project is related to exploring molecular basis of low chill apple cultivars with a focus on how genetic and regulatory mechanisms of low chill apple cultivars influence... Read More →
Co-authors
JS

Jugpreet Singh

University of Florida, Tropical Research and Education Center
NA
KF

Kevin Folta

University of florida
NA
Tuesday July 29, 2025 8:45am - 9:00am CDT
Foster 2
  Oral presentation, Genetics and Germplasm 1

9:00am CDT

GG 1 - Genome-wide study of Salt Tolerance in USDA Tomato Germplasm at Seedling Stage
Tuesday July 29, 2025 9:00am - 9:15am CDT
Salinity is a major constraint on tomato crop production and is increasingly intensified by changing climate conditions. This study aimed to develop superior salt-tolerant tomato cultivars by evaluating genetic variation in salt tolerance, identifying associated single-nucleotide polymorphism (SNP) markers through genome-wide association studies (GWAS), and performing genomic prediction (GP). A total of 265 tomato accessions from the USDA germplasm collection were evaluated at the seedling stage under controlled greenhouse conditions with saline stress (200 mM NaCl). Nineteen accessions were identified as salt-tolerant, exhibiting leaf injury scores ≤3.0 (on a 1–5 scale) and chlorophyll reduction of
Speakers
avatar for Ainong Shi

Ainong Shi

Associate Professor, University of Arkasnas
Dr. Ainong Shi is a faculty member in the Department of Horticulture at the University of Arkansas. His research laboratory specializes in plant breeding and genetics, particularly focusing on vegetable crops such as arugula, cowpea, and spinach for cultivar and germplasm development... Read More →
Co-authors
HX

haizheng xiong

University of Arkansas
HA

Hanan Alkabkabi

university of arkansas
NA
IA

Ibtisam Alatawi

University of Arkansas
NA
KL

Kai-Shu Ling

USDA-ARS
NA
KC

Kenani Chiwina

University of Arkansas
NA
QL

Qun Luo

university of arkansas
NA
Tuesday July 29, 2025 9:00am - 9:15am CDT
Foster 2

9:15am CDT

GG 1 - Genome-wide Association Study of Salt Tolerance at the Seedling Stage in Lettuce
Tuesday July 29, 2025 9:15am - 9:30am CDT
Lettuce (Lactuca sativa L.) is one of the most important leafy vegetable crops worldwide. Soil salinity adversely affects lettuce production leading to considerable yield losses. Identification of genetic loci controlling salt tolerance will facilitate molecular marker development and thereby assist breeders in developing lettuce cultivars with salt tolerance. Accordingly, we conducted a genome-wide association study (GWAS) to identify marker-trait association for salt tolerance at the seedling stage using 409 diverse lettuce accessions and 56,820 high-quality single nucleotide polymorphism (SNP) markers obtained through genotype-by-sequencing technology. Several statistical models, including GLM, MLM, FarmCPU, and BLINK were employed using the GAPIT version 3 software tool for GWAS. Based on three important seedling stage traits affected by salinity, i.e., shoot fresh weight (FW), shoot dry weight (DW) and chlorophyl index (SPAD), 13 significant salt tolerance related SNPs representing 10 QTLs were identified on lettuce chromosomes 1, 3, 4, 6, 7, 8 and 9. Notably, a major QTL on chromosome 4, encompassing four significant SNPs within a 116 bp region of the lettuce reference genome (v8), explained 49% of the phenotypic variation for FW. The identified salt tolerance-related QTLs provide a valuable resource for developing assays for marker-assisted selection to breed lettuce cultivars with improved salt tolerance.
Speakers
MD

Modan Das

USDA-ARS
Co-authors
BM

Beiquan Mou

USDA-ARS
NA
IS

Ivan Simko

USDA-ARS
NA
Tuesday July 29, 2025 9:15am - 9:30am CDT
Foster 2

9:30am CDT

GG 1 - Transcriptomics reveals candidate genes associated with high temperature stress tolerance in chile pepper (Capsicum annum)
Tuesday July 29, 2025 9:30am - 9:45am CDT
Chile pepper (Capsicum annuum) is widely produced and consumed, but farmers face significant challenges associated with high temperature stress. Tolerance to high temperatures is a phenotype comprised of numerous component traits each of which contribute to the overall performance of the plant. Our aim was to identify the key mechanisms associated with heat stress response in the leaves and in the floral organs of chile pepper. One-month-old plants of heat-sensitive (AVPP1609-038) and -tolerant (AVPP1609-015) recombinant inbred line (RIL) of chile pepper were subjected to heat stress (38 and 28°C day and night temperatures) and control conditions (32 and 24°C day and night temperatures) in growth chambers with a 14-hour photoperiod. Leaf and floral bud samples were collected for RNA extraction at 11 and 18 days after treatment, respectively, with four biological replicates per tissue. Differentially expressed genes (DEGs) were identified by comparing tolerant and sensitive RILs across treatments and tissues. For the heat-tolerant AVPP1609-015 under heat stress, 1,118 DEGs were identified, with 649 specific to floral buds, 381 in leaves, and 88 shared between the two tissues. Biological processes such as RNA splicing and heat acclimation were predominantly upregulated in floral buds, while lipid catabolism was enhanced in leaves. Developmental processes were consistently suppressed in both tissues for the RILs under heat stress conditions. For the heat-tolerant AVPP1609-015 nuclease activity was strongly suppressed, likely to preserve nucleic acid integrity under heat stress. Hormonal regulation showed tissue specificity, with salicylic acid playing a pivotal role in leaves and ethylene in floral buds, potentially associated with flower abscission. Additionally, key transcription factors associated with heat tolerance were identified. While some mechanisms of heat tolerance were shared between tissues, distinct responses were observed as well, suggesting the need for different breeding approaches to enhance heat tolerance in vegetative and reproductive tissues of chile pepper. These findings provide valuable insights for developing heat-resilient chile pepper and a foundation for future research.
Speakers
avatar for Derek Barchenger

Derek Barchenger

World Vegetable Center
Co-authors
FC

Fang-Min Chang

National Chung Hsing University
NA
JO

Jesish Ojha

World Vegetable Center
NA
LA

Lourena Arone Maxwell

World Vegetable Center
NA
MY

Ming-Ren Yen

National Chung Hsing University
NA
SK

Swaroop KO

University of Agricultural Sciences, Dharwad
NA
YH

Yu-Ming Hsu

Université Paris-Saclay
NA
YN

Yu-Shin Nai

National Chung Hsing University
NA
Tuesday July 29, 2025 9:30am - 9:45am CDT
Foster 2
  Oral presentation, Genetics and Germplasm 1
  • Subject Genetics and Germplasm
  • Funding Source This work was funded by APSA-WorldVeg Consortium Members and the long-term strategic donors to the World Vegetable Center: Taiwan, US Agency for International Development (USAID), Australian Centre for International Agricultural Research (ACIAR), Germany, Thailand, Philippines, Korea and Japan.

9:45am CDT

GG 1 - Chemical and CRISPR-Induced Mutagenesis for Herbicide Tolerance in Plants: From Agronomic to Horticultural Crops
Tuesday July 29, 2025 9:45am - 10:00am CDT
Herbicide tolerance in plants is an increasingly valuable trait due to the high labor and costs associated with weed control in agriculture. Herbicide application remains the most effective and widely used weed management strategy, making the development of tolerant plants essential. First discovered in the 1970s and commercially grown since 1984, herbicide resistant crops have become a key tool in agriculture, with increasing demand for new tolerant varieties. Chemical mutagenesis and CRISPR-mediated gene editing have been used to induce mutations and develop herbicide tolerant plants. Chemical mutagenesis involves treating plant tissue with mutagens such as ethyl methanesulfonate (EMS) to induce random mutations, followed by screening to identify tolerant mutants. This conventional approach has played a significant role in breeding programs and remains widely used for developing herbicide tolerant crops. EMS mutagenesis has successfully generated ALS-resistant varieties in several agronomic crops, including Clearfield® maize, rice, and wheat, which are resistant to imidazolinone (IMI) herbicides without being classified as genetically modified (GM). It is particularly effective for developing crops resistant to ALS- and ACCase-inhibiting herbicides, as these mutations typically require only minor changes in the target genes. CRISPR-mediated gene editing, using tools such as CRISPR-Cas9, base editing (CBE, ABE), and prime editing, enables precise modifications in plant genomes to confer herbicide tolerance. These advancements have revolutionized crop development through their efficiency, precision, and cost-effectiveness. By targeting herbicide receptor genes such as ALS, ACCase, and EPSPS, CRISPR-based systems have produced herbicide tolerant varieties in several agronomic crops. CRISPR is particularly valuable for engineering tolerance to non-selective herbicides, such as glyphosate, due to the complex genomic architecture of the EPSPS gene. Chemical mutagenesis facilitates the discovery of novel mutations and is particularly useful in understudied species lacking the genomic information required for CRISPR-based modification. In contrast, CRISPR-based genome editing provides a highly precise and efficient method for developing herbicide tolerant crops, especially when targeting complex genes. Integrating chemical mutagenesis with CRISPR-mediated gene editing expands the range of available herbicide tolerance traits and offers new opportunities for sustainable weed management. These advances in agronomic crops provide a strong foundation for extending herbicide tolerance studies to horticultural and specialty crops, where research has been more limited despite similar weed management issues.
Speakers
MM

Mase Merkel

University of Florida
Co-authors
ZD

Zhanao Deng

University of Florida
Tuesday July 29, 2025 9:45am - 10:00am CDT
Foster 2
  Oral presentation, Genetics and Germplasm 1
  • Subject Genetics and Germplasm
  • Funding Source USDA Hatch Project FLA-GCC-006190; USDA AMS Specialty Crop Block Grant through the Florida Department of Agriculture and Consumer Services (FDACS) Specialty Crop Block Grant Program

11:00am CDT

Viticulture and Small Fruits Interest Group Business Meeting
Tuesday July 29, 2025 11:00am - 12:00pm CDT
Open to all attendees.


Tuesday July 29, 2025 11:00am - 12:00pm CDT
Foster 2

1:45pm CDT

IPPM for Controlled Environment Horticulture Crops (Interest Group Session)
Tuesday July 29, 2025 1:45pm - 3:45pm CDT
Developing integrated pest and pathogen management approach for controlled environment horticulture crops is of major impotance to safeguard growers interest, and community health and sustainability. The abilty to control temperature, light and to some extent humidity, creates optimal conditions for crop growth. However, the same suitable environment creates favorable opportunities for insect pest population growth, their associated viral diseases and fungal pathogens. Growers often rely on chemical insecticides to control CEH pests, leading to selective pressure and development of insecticide resistance in major CEH pests, such as aphids, thrips and whitelfies. This highlights the fact that insecticides are not reliable long-term pest control solution. Therefore, CEH specific effective pest and pathogen management approaches are urgently needed to move towards sustainable CEH crop production nationwide.

Speakers:
  • Samantha Wilden, Cornell University
  • Arash Kheroiding, Texas A&M AgriLife
  • Krishna Bhattarai, Texas A&M AgriLife
  • Erich Schoeller, University of Georgia
  • Ruchika Kashyap, University of Georgia
  • Andrew Ogden, University of Georgia
  • Azlan Zahid, Texas A&M  AgriLife
Tuesday July 29, 2025 1:45pm - 3:45pm CDT
Foster 2

3:59pm CDT

CITR 1 - Oral Session
Tuesday July 29, 2025 3:59pm - 4:00pm CDT
Presiding/Moderator
avatar for Caroline de Favari Tardivo

Caroline de Favari Tardivo

PhD Candidate, University of Florida
Tuesday July 29, 2025 3:59pm - 4:00pm CDT
Foster 2

4:00pm CDT

CITR 1 - Delaying Candidatus Liberibacter asiaticus Infection of Citrus Trees Through Use of Individual Protective Covers and Systemic Delivery of Oxytetracycline
Tuesday July 29, 2025 4:00pm - 4:15pm CDT
Huanglongbing (HLB), associated with Candidatus Liberibacter asiaticus (CLas), continues to devastate citrus production worldwide. Individual protective covers (IPCs) offer a physical barrier against the Asian citrus psyllid, delaying CLas infection in young trees. However, once removed, trees become vulnerable to rapid infection. This study evaluates an integrated approach utilizing IPCs followed by systemic oxytetracycline (OTC) trunk injections to mitigate disease progression and sustain tree health. A field trial was established with ‘Valencia’ sweet orange (Citrus sinensis) grafted onto US-812 or US-942 rootstocks. Trees were either protected with IPCs for 18 months after planting (“late infected”) or had “open” IPCs (“early infected”). All trees received annual OTC injections in 2023 and 2024, beginning 10 months after IPC removal. CLas titers, OTC residues, tree growth, fruit yield and quality, leaf nutrient content, and root endorhizosphere microbial dynamics were assessed. Results indicate that IPCs successfully delayed infection, as late-infected trees maintained lower CLas titers for the first five months after IPC removal and exhibited denser canopies and larger scion and rootstock trunk diameters than early-infected trees. OTC injections significantly reduced CLas titers in leaves, with effects persisting for up to six months. However, no significant reductions in CLas titers were detected in fibrous roots, suggesting limited OTC translocation to the root system. Injected trees exhibited a greener canopy color, greater leaf size, higher chlorophyll content, higher yield, larger fruit size, and better juice quality compared to non-injected trees. No significant changes in leaf nutrient content were observed, and the root endorhizosphere microbial diversity remained unchanged across treatments, indicating minimal disruption to the microbial community due to the antibiotic. These findings provide evidence supporting the combination of IPCs and systemic delivery of OTC as an integrated strategy to manage HLB in new citrus plantings. IPCs delay the onset of infection, allowing trees to establish a healthy canopy and robust root system, while OTC injections aid in sustaining tree health post-infection. The integration of these practices provides a promising framework for establishing productive citrus orchards under HLB-endemic conditions.
Speakers
avatar for Caroline de Favari Tardivo

Caroline de Favari Tardivo

PhD Candidate, University of Florida
Co-authors
BM

Brittney Monus

University of Florida
NA
FA

Fernando Alferez

University of Florida
LA

Leigh Archer

University of Florida
NA
SS

Sarah Strauss

University of Florida/IFAS, Southwest Florida Research and Education Center
NA
UA

Ute Albrecht

University of Florida/IFAS, Southwest Florida Research and Education Center
Tuesday July 29, 2025 4:00pm - 4:15pm CDT
Foster 2
  Oral presentation, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source USDA NIFA ECDRE 2022-70029-38481 / 2020-70029-33202
  • Funding Option SCRI funded all or part of the research associated with this abstract

4:15pm CDT

CITR 1 - Enhancing efficacy of oxytetracycline trunk injection for Huanglongbing management in citrus: effects of volume, mode, and time of injection
Tuesday July 29, 2025 4:15pm - 4:30pm CDT
Huanglongbing (HLB), associated with phloem-limited bacteria Candidatus Liberibacter asiaticus (CLas), is a major threat to citrus production worldwide, severely reducing tree health, yield, and fruit quality. In Florida, trunk injection of oxytetracycline (OTC) has emerged as a promising management strategy for HLB. Under this context, this study aimed to enhance the efficacy of OTC injection by evaluating different injection volumes, modes of injection (one or two-sided), and injection timing in HLB-affected ‘Valencia’ orange (Citrus sinensis) trees in southwest Florida. Two experiments were conducted with 8-year-old trees. The first experiment, implemented in two locations, assessed four combinations of different OTC volumes and modes of injection: 1) 50 mL (one-sided), 2) 100 mL (one-sided), 3) 100 mL (two-sided, 50 mL each side), and 4) 200 mL (two-sided, 100 mL each side), all delivering 0.825 g (a.i.) OTC (Rectify, AgroSource, Inc.) per tree. The second experiment compared early (May) and late (July) injections using 100 mL (two-sided, 50 mL each side), delivering 0.825 g (a.i.) OTC (Rectify) per tree. Injectors were placed on opposite sides of the trunk for the two-sided treatments. OTC solutions were prepared right before injection. In both experiments, treatments were compared against an untreated control. The experimental design was completely randomized, with 10 single-tree replications for the first experiment and 8 replications of 4 trees each for the second. Leaf samples were collected over time to analyze bacterial (CLas) titers and OTC residues, while canopy health, yield, and fruit quality were evaluated annually. For the second experiment, fruit development (size and peel color) was monitored monthly after OTC injection, and fruit yield and quality were assessed annually. Our results demonstrate that two-sided injection increases fruit yield more than one-sided injection and that the combination of two-sided injection and a larger volume of the injected solution may further enhance fruit yield compared to the untreated control. This improvement is attributed to better OTC distribution within the canopy, leading to greater bacterial suppression and tree recovery. Moreover, early (May) injection promotes fruit size by extending the fruit growth period, while late (July) injection enhances juice soluble solids content with a more compact fruit size. However, early injections result in greener fruit, which requires a longer maturation period to achieve the desired peel color and sugar content. These findings offer valuable insights for optimizing OTC trunk injection strategies to improve citrus health and productivity in HLB-affected citrus orchards.
Speakers
DU

Deived Uilian de Carvalho

University of Florida
Co-authors
CD

Caroline de Favari Tardivo

University of Florida
JD

Jasmine de Freitas

University of Florida
NA
UA

Ute Albrecht

University of Florida/IFAS
Tuesday July 29, 2025 4:15pm - 4:30pm CDT
Foster 2
  Oral presentation, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source USDA NIFA ECDRE (2021-70029-36056), Citrus Research and Development Foundation (22-001 and 23-002)

4:30pm CDT

CITR 1 - Rehabilitation of young HLB-affected citrus trees through combination of IPCS and OTC trunk injection
Tuesday July 29, 2025 4:30pm - 4:45pm CDT
The combined use of Individual Protective Covers (IPCs) and oxytetracycline (OTC) trunk injection offers a promising strategy for managing huanglongbing (HLB) in young citrus trees by reducing environmental stress and preventing Candidatus Liberibacter asiaticus (CLas) inoculation. This study evaluated whether young citrus trees that were grown without IPCs for the first two years after planting and severely affected by HLB can be rehabilitated by OTC injections, and whether IPCs provide additional benefits beyond OTC alone. ‘Valencia’ sweet orange trees on US-812 and US-942 rootstocks, were organized in a randomized complete block design with three treatments: 1) no injection no IPC, 2) OTC injection no IPC, and 3) OTC injection IPC. Each treatment-rootstock combination consisted of 14 single-tree replications. Trunk injections were performed annually for two years (2023-2024) using Chemjet tree injectors, with solutions delivered into the rootstock. Comprehensive data were collected on the yield, juice quality, bacterial titers, disease state, flushing patterns, and psyllid infestation. No significant differences were observed between the rootstocks except for the juice quality, which was better in combination with US-812. OTC-injected trees consistently exhibited higher yields and improved juice quality, with no additional benefits from IPCs. A significant decrease in CLas titers was noted starting three months post-injection. Injected trees under IPCs had fewer HLB symptoms in the second year, and injected trees exhibited a significant increase in canopy density compared with non-injected trees. Trees with IPCs flushed less frequently during summer and winter than trees without IPCs. The psyllid infestation rate was the same in injected and non-injected trees, indicating that OTC treatment does not affect host attractiveness. These findings highlight the potential of OTC trunk injection to rehabilitate young HLB-affected citrus trees and enhance tree productivity. The longer-term role of IPCs in young tree rehabilitation is still under investigation.
Speakers
avatar for Larissa Nunes da Silva

Larissa Nunes da Silva

UF/IFAS - Southwest Florida Research
Co-authors
UA

Ute Albrecht

University of Florida/IFAS
Tuesday July 29, 2025 4:30pm - 4:45pm CDT
Foster 2
  Oral presentation, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source USDA NIFA ECDRE 2022-70029-38481
  • Funding Option SCRI funded all or part of the research associated with this abstract

4:45pm CDT

CITR 1 - Evaluation of Different Oxytetracycline Doses Delivered by Trunk Injection for HLB Management of Mature ‘Valencia’ Sweet Orange Trees
Tuesday July 29, 2025 4:45pm - 5:00pm CDT
The systemic delivery of oxytetracycline (OTC) by trunk injection has emerged as a viable strategy to diminish the impacts of the bacterial disease Huanglongbing (HLB) in Florida. This study aimed to determine the optimal OTC dose and best time of delivery to enhance tree health and productivity in mature HLB-affected citrus trees. It also investigated whether OTC effects persist after one single injection compared to two consecutive annual injections. The study began in 2023 at a commercial citrus grove in Polk County, Florida, using 18-year-old ‘Valencia’ (Citrus sinensis) trees grafted onto Swingle (C. paradisi × Poncirus trifoliata) rootstock. Four OTC doses (0.55 g, 0.825 g, 1.1 g, and 1.65 g of active ingredient per tree) were evaluated alongside two application times, spring and late summer, with a non-injected control for comparison. The experiment was a randomized complete block design with eight replications containing four trees. A commercial OTC formulation (ReMedium TI, 95% oxytetracycline hydrochloride) registered in Florida was applied using commercial tree injectors (FlexInject). Doses were applied in 100 ml acidified water through a single injector, except for the highest dose, which was split between two injectors and applied in a volume of 75 ml on opposite sides of the trunk. All trees received their designated doses in year 1, except for the non-injected controls. Only half of the trees injected in year 1 received a second dose in year 2. Trees were harvested in March 2024 and February 2025. Fruits were collected for fruit and juice quality analysis and to determine OTC fruit residue levels. Regardless of the dose, all OTC-treated trees produced more, larger-sized fruits, more total soluble solids (TSS), and a higher TSS-to-acid ratio compared to non-injected controls. The best results were obtained with the highest dose applied by split injections. There were no major differences between spring and summer injections except for the TSS content, which was higher and the percent acid, which was lower when trees were injected in late summer. However, fruit OTC residues were above the allowed maximum after late summer injections, suggesting that injections should be completed by the end of spring. Trees injected for two consecutive years produced significantly more, better-quality fruits than trees injected only once in the first year. These results confirm the efficacy of OTC trunk injections to enhance productivity and fruit quality under HLB-endemic conditions and provide important guidelines regarding dose and timing of injections.
Speakers
avatar for Gabriel Pugina

Gabriel Pugina

PhD candidate, University of Florida
Co-authors
CD

Caroline de Favari Tardivo

University of Florida
UA

Ute Albrecht

University of Florida/IFAS
Tuesday July 29, 2025 4:45pm - 5:00pm CDT
Foster 2
  Oral presentation, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source Florida Citrus Research and Development Foundation (CRDF) #23-002

5:00pm CDT

CITR 1 - The Effects of Rootstock Selection and Oxytetracycline Trunk Injection on the Fruit and Juice Quality of the 'OLL-8' Sweet Orange Scion
Tuesday July 29, 2025 5:00pm - 5:15pm CDT
Citrus production in the State of Florida has been decimated by the disease Huanglongbing (HLB). This disease is associated with the phloem-limited, gram-negative bacteria Candidatus Liberibacter asiaticus (CLas) and is vectored by the Asian citrus psyllid (Diaphorina Citri). Since HLB was first identified in Florida in 2005 it spread rapidly and was considered endemic in the state by 2013, resulting in a nearly 90% decline in citrus fruit production. While breeding more tolerant or fully resistant cultivars is likely the best long-term solution, trunk injections of the antibiotic oxytetracycline were granted emergency use approval in October 2022 to combat the bacteria. Historically most of Florida’s citrus production has been sweet orange (Citrus sinensis (L.) Osbeck) cultivars including ‘Hamlin’ and ‘Valencia’ which were primarily destined for juice processing. Unfortunately, all known commercial sweet orange cultivars are susceptible to HLB infection leading to the rapid and drastic decrease in production across the state. Of the sweet orange cultivars that have been released by the University of Florida Institute of Food and Agricultural Sciences (UF-IFAS), Orie and Louise Lee (‘OLL’) lines have shown some degree of tolerance to HLB in cultivar field trials. In our experiment we evaluated the effects of rootstock selection and oxytetracycline (OTC) trunk injections on the fruit and juice quality of the ‘OLL-8’ sweet orange scion. Three of the evaluated rootstocks (‘US-942’, ‘US-897’, and ‘Swingle’) are industry standard diploids, while the other three rootstocks (‘UFR-2’, ‘UFR-4’, and ‘UFR-6’) are tetraploids released by the University of Florida. For each of these rootstocks there were trees that were injected with OTC and untreated control trees. Fruit were harvested at several time points and data were collected on juice quality attributes including titratable acidity in citric acid equivalents, total soluble solids, sugar/acid ratio, pounds-solids per box, and juice color, as well as fruit characteristics including mass, diameter, total yield, and premature fruit drop. The trees that were injected with OTC had significantly larger mean fruit diameter, higher mean ratio, and lower premature fruit drop than untreated controls. There was also evidence for statistically significant differences among rootstock selections in conjunction with the ‘OLL-8’ scion for each of the characteristics that were evaluated. These results indicate that rootstock selection and OTC-trunk injections can significantly improve fruit and juice quality of the ‘OLL-8’ sweet orange scion. However, more research is needed on the effects of OTC injections in different rootstock-scion combinations.
Speakers
JV

Joe Volpe

University of Florida
Co-authors
EW

Emily Worbington

University of Florida-IFAS CREC
JC

John Chater

University of Florida
Tuesday July 29, 2025 5:00pm - 5:15pm CDT
Foster 2
  Oral presentation, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source USDA Agricultural Marketing Service (AMS) MultiState Specialty Crop Block Grant through the California Department of Food and Agriculture project number 19-1043-002-SF

5:15pm CDT

CITR 1 - Efficacy of Oxytetracycline (OTC) for Huanglongbing (HLB) Management in South Texas ‘Rio Red’ Grapefruit
Tuesday July 29, 2025 5:15pm - 5:30pm CDT
Citrus Huanglongbing (HLB), also known as citrus greening, is associated with Candidatus Liberibacter asiaticus (CLas), a phloem-limited bacterium transmitted by an insect vector, Asian Citrus Psyllid (ACP). Currently, there are no commercially viable treatment options available for HLB management. Trunk injection of Oxytetracycline (OTC) has been widely adopted as a treatment option in Florida. Currently, OTC is not approved for use in Texas citrus, and no OTC efficacy data is available. This study evaluated the efficacy of OTC in mitigating HLB and managing tree health in nine and 17-year old ‘Rio Red’ grapefruit (Citrus paradisi Macfadyen) trees on sour orange (Citrus aurantium L.) rootstock, on a randomized complete block design in two commercial orchards. RECTIFY™ (95% Oxytetracycline hydrochloride) was injected into tree trunks at two rates (0.55g and 1.1g per tree) using Chemjet® tree injectors during June-July 2023 and May 2024. Trees were evaluated for various attributes, including periodic (pre-treatment, three, six, and nine months after OTC injection) bacterial titer in leaf and root tissues and tree health monitoring (canopy color, canopy density, and HLB severity) for the same time intervals. Fruits collected at harvest maturity were evaluated for yield, fruit size, and juice quality (brix, titratable acidity, flavonoids, carotenoids). Additionally, pre-harvest fruit drop and trunk injection site damage were recorded. OTC residue was measured in whole fruit and juice using Ultra-High-Performance Liquid Chromatography coupled with tandem Mass Spectrometry (UHPLC-MS-TOF). Nine-year-old trees treated with 1.1g of OTC/tree in one of the sites showed a significant reduction of bacterial titers in leaf tissue. OTC treatment did not significantly improve yield. Canopy color showed noticeable improvement after the second OTC injection in the sites, whereas canopy density remained largely unaffected. After the second OTC injection, HLB severity and pre-harvest fruit drop were reduced, and improvement in fruit size was observed in 9-year-old trees. There is no significant improvement in juice quality. Damage caused by trunk injections healed rapidly in older trees than in younger trees. No OTC residue was detected in the juice and whole fruit at six months after the OTC injection. This is the first report on the efficacy of OTC for HLB management in Rio Red grapefruit in South Texas.
Speakers Co-authors
AS

Aditi Satpute

Texas A
NA
JP

Jong-Won Park

Texas A
NA
RM

Ram Mohan Uckoo

Wonderful Citrus
NA
RG

Ruth Galan

Wonderful Citrus
NA
Tuesday July 29, 2025 5:15pm - 5:30pm CDT
Foster 2

5:30pm CDT

CITR 1 - Lessons from Oxytetracycline Trunk Injection to Manage Citrus Huanglongbing in Florida
Tuesday July 29, 2025 5:30pm - 5:45pm CDT
Huanglongbing (HLB, aka citrus greening) has devastated citrus production around the world, especially in Florida where the disease has been endemic since 2013. HLB is associated with phloem-limited bacteria, Candidatus Liberibacter asiaticus, vectored by the Asian citrus psyllid, Diaphorina citri. Elimination of the disease vector is difficult because of its abundance and the high costs of frequent insecticide applications. Foliar applied therapies to treat infected trees are largely ineffective because of the systemic distribution of the bacteria. Trunk injection of oxytetracycline (OTC) has emerged as a possible therapy to systemically deliver and target the pathogen and restore tree health. Since the registration of trunk injection of OTC for commercial use in Florida, our lab has conducted numerous field studies to assess the efficacy of this therapy on a large scale and study the influence of various factors such as tree age, rootstock, OTC dose, and time and method of application. Overall, all studies revealed significant improvements in fruit production along with increases in fruit and juice quality after one year of application. Yield improvements after two years of application were cumulative, reaching up to two-fold or more. The magnitude of improvements depended on various factors. Young trees generally responded better than older trees, and tree responses varied based on the rootstock. The efficacy of the therapy also depended on the dose of OTC administered to the trees, with higher doses generally generating larger effects. Moreover, delivering OTC on multiple sides of a tree instead of on only one side enhanced its distribution and therefore, efficacy. Late season injections improved the juice quality more but increased the OTC residue content in the fruits. The currently allowed residue level is 10 ppb, which was not exceeded when injections occurred in spring or early summer. OTC is phytotoxic and can have adverse effects on the tree. Efforts are underway to reduce or eliminate these negative effects and improve efficacy. Alternative therapies to be used instead of, or in combination with OTC, are also under evaluation. Taken together, the systemic delivery of OTC by trunk injection has emerged as a viable and economically feasible therapy to manage HLB in Florida and has been widely adopted. Nevertheless, it is regarded as a short-term solution until other solutions become available.
Speakers
UA

Ute Albrecht

University of Florida/IFAS
Co-authors
CD

Caroline de Favari Tardivo

University of Florida
DD

Deived de Carvalho

University of Florida/IFAS
GM

Gerardo Moreno

University of Florida/IFAS
NA
IR

Igor Rodrigues

University of Florida/IFAS
JD

Jasmine de Freitas

University of Florida/IFAS
NA
LN

Larissa Nunes da Silva

UF/IFAS - Southwest Florida Research
Tuesday July 29, 2025 5:30pm - 5:45pm CDT
Foster 2
  Oral presentation, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source USDA NIFA ECDRE (project 2021-70029-36056) and Citrus Research and Development Foundation (projects 22-001, 23-002, 23-005, 23-036)
  • Funding Option SCRI funded all or part of the research associated with this abstract

5:45pm CDT

CITR 1 - Optimizing Trunk Injections of Oxytetracycline for Citrus Huanglongbing Management
Tuesday July 29, 2025 5:45pm - 6:00pm CDT
In Florida and worldwide, citrus production is threatened by Huanglongbing (HLB), associated with phloem-limited Candidatus Liberibacter asiaticus (CLas). Oxytetracycline delivery by trunk injection has been approved to treat commercial citrus groves affected by this endemic disease. However, the commercially available formulations require acidification of the active ingredient (oxytetracycline hydrochloride, OTC) to a pH of 1.8-2.0 to dissolve and stabilize the antibiotic, which may cause trunk damage, chloride toxicity, and limits compatibility with other compounds. A novel adjuvant has been developed, using EPA-approved ingredients to dissolve and stabilize OTC at a neutral pH while maintaining efficacy. This study compares the efficacy of neutralized and acidified solutions of OTC with and without the addition of ZnSO4 to optimize HLB management. The efficacy of Streptomycin (STM) as an alternative to or combined with OTC was also assessed. A field trial was conducted in a commercial citrus orchard in southwest Florida using seven-year-old ‘Valencia’ sweet orange trees grafted on X-639 rootstock. The experimental design was a randomized block with ten treatments and eight replications, each containing four trees. Treatments included combinations of OTC, STM, and ZnSO4 with or without the adjuvant, and appropriate controls. Trees were injected in June 2024 with 100 mL of treatment solution on one side of the trunk using commercial (FlexInject, TJ BioTech) injectors. Leaves were collected to assess CLas titers and bacterial suppression. Phytotoxic effects in the canopy and trunk damage at the injection site were evaluated. Treatments containing the adjuvant and/or OTC caused significant phytotoxicity and more trunk damage. Trees were harvested in March 2025. Fruit yield was higher for all treatments containing OTC compared to the controls . The highest yield was measured when trees were injected with a combination of OTC and STM. The findings of this study led to the redesign of the neutral pH adjuvant to minimize tree damage and enhance OTC efficacy. The optimized formulation will be tested using the same experimental design in upcoming studies.
Speakers
avatar for Igor Rodrigues da Silva

Igor Rodrigues da Silva

Master's Student, UF/IFAS Southwest Florida Research
Co-authors
JP

Jorge Pereira

NanoScience Technology Center
NA
SS

Swadeshmukul Santra

University of Central Florida
NA
UA

Ute Albrecht

University of Florida/IFAS
Tuesday July 29, 2025 5:45pm - 6:00pm CDT
Foster 2
  Oral presentation, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source Citrus Research and Development Foundation (23-036)
 
Wednesday, July 30
 

7:59am CDT

VGBR 1 - Oral Session
Wednesday July 30, 2025 7:59am - 8:00am CDT
Presiding/Moderator
CA

Carlos Avila

Texas A&M University
Wednesday July 30, 2025 7:59am - 8:00am CDT
Foster 2

8:00am CDT

VGBR 1 - Association mapping of Fruit Quality traits in Tomato (Solanum lycopersicum)
Wednesday July 30, 2025 8:00am - 8:15am CDT
Tomato fruit quality is directly related to marketability and consumer appeal. Unfortunately, consumers are increasingly discontent with the flavor and quality of the product they are purchasing and requesting tomatoes with improved flavor, aroma, texture, and appearance making it a high priority for breeding programs. Traditionally, the selection for fruit quality traits in breeding programs has been done using conventional phenotyping approaches, in which large populations need to be screened. Although this approach has resulted in the release of high quality-high yielding cultivars, it is very time-consuming, delaying cultivar release. An alternative approach to improve breeding efficiency involves the use of modern molecular breeding techniques. In this study, a diverse panel of 305 tomato genotypes, including 284 tomato breeding lines developed by Texas A
Speakers
CA

Carlos Avila

Texas A&M University
Co-authors
DK

Devi Kandel

Langston University
KC

Kevin Crosby

Texas A
NA
Wednesday July 30, 2025 8:00am - 8:15am CDT
Foster 2

8:15am CDT

VGBR 1 - Identification of resistance loci for Septoria leaf spot in tomatoes
Wednesday July 30, 2025 8:15am - 8:30am CDT
Septoria Leaf Spot (SLS), caused by the fungal pathogen Septoria lycopersici, is a highly destructive foliar disease affecting tomatoes. SLS is most severe in the Northeast USA and North Carolina during periods of high humidity and elevated temperatures, which can lead to catastrophic yield loss. No quantitative trait loci (QTL) associated with SLS resistance have been reported. Therefore, the objective of this study was to map the QTL related to SLS resistance in tomatoes. An F2:4 mapping population consisting of 189 individuals derived from NC123S (susceptible) x Wisconsin 55 (moderately resistant) was assessed under both field and greenhouse conditions through artificial inoculation with a spore concentration of 15.3 x 10^4/mL at the Mountain Horticultural Crops Research and Extension Center, Mills River, NC, and Mountain Research Station, Waynesville, NC. The population was genotyped using the SPET (single primer enrichment technology) Allegro targeted genotyping method. SPET-derived SNP (single nucleotide polymorphism) molecular markers were used to construct a linkage map spanning 3810.2 cM. QTL analysis identified 12 QTLs associated with SLS resistance, including two major effects and ten minor effects, typical for at least two environments identified across the genome, explaining phenotypic variation (R² value) ranging from 3.7% to 13.5%. These results demonstrate that the genetic control of SLS resistance is polygenic. This study may provide a foundation for understanding the genetics of SLS resistance and marker-assisted selection (MAS) for transferring SLS resistance genes into elite tomato breeding lines.
Speakers
DP

Dilip Panthee

North Carolina State University
Co-authors
JB

Jackie Blume

North Carolina State University
NA
MI

Muhammad Irfan Siddique

North Carolina State University
Wednesday July 30, 2025 8:15am - 8:30am CDT
Foster 2

8:30am CDT

VGBR 1 - Evaluation of Drought Tolerance in USDA Tomato USDA Germplasm Using Morphological and Physiological Approaches
Wednesday July 30, 2025 8:30am - 8:45am CDT
Drought is a major abiotic stressor that significantly reduces the growth and yield of tomato (Solanum lycopersicum L.). To mitigate its adverse effects, the development and utilization of drought-tolerant cultivars, combined with advanced breeding strategies, offer sustainable solutions. In this study, a total of 157 USDA tomato accessions were evaluated under controlled greenhouse conditions using a randomized complete block design with four replications, incorporating both a water-deficit treatment and a well-watered control group. The results identified ten accessions, including PI 487624, PI 127828, PI 379018, PI 365903, PI 390515, PI 390663, PI 128657, PI 266376, PI 126444, and PI 298933, as drought tolerant, with leaf wilting and leaf rolling scores of less than four. Broad-sense heritability estimates ranged from 50 percent to approximately 58 percent, indicating a moderate genetic influence on drought tolerance. Correlation analysis revealed strong positive associations ranging from 0.50 to 0.99 among leaf wilting, leaf rolling, plant freshness, leaf thickness, and SPAD chlorophyll content, while negative correlations ranging from -0.40 to -0.81 were observed for plant fresh weight, leaf thickness, Quantum yield of Photosystem II (Phi2), and SPAD chlorophyll content traits. These findings provide valuable insights into tomato breeding programs focused on improving drought resilience in elite cultivars. We plan to expand the evaluation to a broader set of accessions and employ genome-wide association studies and genomic prediction to identify single nucleotide polymorphism markers and candidate genes associated with drought tolerance. The integration of genome-wide association studies and genomic prediction will facilitate marker-assisted selection and genomic selection, improving the efficiency of breeding programs aimed at developing drought-resilient tomato cultivars.
Speakers
KC

Kenani Chiwina

University of Arkansas
NA
Co-authors
AS

Ainong Shi

University of Arkasnas
AR

Awais Riaz

University of Arkansas
NA
BM

Beiquan Mou

Sam Farr U.S. Crop Improvement and Protection Research Center, U.S. Dept. of Agriculture, Agricultural Research Service (USDA-ARS)
NA
DH

Derrick Harrison

university of arkansas
NA
HX

haizheng xiong

University of Arkansas
HA

Hanan Alkabkabi

university of arkansas
NA
IA

Ibtisam Alatawi

university of arkansas
NA
MU

Muhammad Ullah

university of arkansas
NA
QL

Qun Luo

university of arkansas
NA
RD

Renjie Du

university of arkansas
NA
YQ

YUEJUN QU

University of Arkansas
Wednesday July 30, 2025 8:30am - 8:45am CDT
Foster 2

8:45am CDT

VGBR 1 - Understanding the molecular basis of Blossom-end rot in tomato
Wednesday July 30, 2025 8:45am - 9:00am CDT
Blossom-end rot (BER) is a physiological disorder in tomatoes that renders the fruit to be unmarketable. In tomato, BER initiates around 7-10 days post anthesis (DPA) at the distal end of the fruit as a water-soaked symptom that can progress into a necrotic lesion covering the entire fruit during development. Calcium deficiency in the distal end of the fruit is thought to trigger BER initiation. In this study, we have used near isogenic lines (NILs) that segregate for BER and harbors quantitative trait loci (QTLs) BER11.1 and BER11.2. Physiological characterization indicated lower calcium concentration and reduced number of vascular bundles in the distal inner and distal pericarp tissue in the susceptible fruit in comparison to the with resistant fruit. To further delineate the molecular mechanisms underlying BER development and to identify the potential candidate gene(s) underlying QTL BER11.1
Speakers
RS

ranveer singh

university of georgia
Co-authors
EV

Esther van der Knaap

Institute of Plant Breeding, Genetics, and Genomics, University of Georgia
NA
MS

Manoj Sapkota

University of Georgia
NA
SN

Savithri Nambeesan

University of Georgia
YT

Yasin Topcu

University of Georgia, Institute of Plant Breeding, Genetics, and Genomics
NA
Wednesday July 30, 2025 8:45am - 9:00am CDT
Foster 2
  Oral presentation, Vegetable Breeding 1
  • Subject vegetable Breeding
  • Poster # csv
  • Funding Source United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA); award number: 2020–67013-30912

9:00am CDT

VGBR 1 - Towards Mechanical Harvest: Leveraging Genomic Selection to Increase Fruit Firmness in Fresh Market Tomato
Wednesday July 30, 2025 9:00am - 9:15am CDT
Fresh market tomato (Solanum lycopersicum) is one of the most important vegetable crops in the US, but this labor-intensive industry faces severe labor shortages and rising production costs amid heavy competition from lower-cost imports. With labor accounting for over 30% of total production expenses, much of which is due to harvesting, the industry's long-term sustainability depends on developing more labor-efficient systems. Mechanical harvesting presents a promising solution, but tomato fruit are highly susceptible to bruising, a challenge that could be amplified by mechanized handling. Fruit firmness plays a crucial role in resistance to internal bruising, making it a key breeding target for improving harvest efficiency and post-harvest quality. The UF/IFAS tomato breeding program has developed tomato lines with traits beneficial for mechanical harvesting, including compact growth habit (CGH) and increased fruit firmness. To investigate the genetic basis of fruit firmness in CGH lines, bi-parental populations were developed from firm and soft inbred parents. Genome-wide association analysis identified multiple minor-effect QTLs, confirming the quantitative nature of this trait in the population. Variance component analysis revealed that fruit firmness is primarily controlled by additive genetic variance, suggesting a strong potential for improvement through selection with appropriate strategies such as genomic selection (GS), which has been successfully used to improve quantitative traits in many crop species. GS models were successfully trained to predict fruit firmness, demonstrating the feasibility of integrating GS into the UF/IFAS tomato breeding program. Model optimization, including adjustments to training population size, marker density, and the incorporation of significant QTLs as fixed effects, improved prediction accuracy and computational efficiency. This study confirms the presence of significant fruit firmness variability in UF/IFAS germplasm, supporting its use in breeding firmer CGH tomatoes suited for mechanical harvest. Future research will refine GS models by incorporating multi-trait and multi-environment analyses, leveraging variance-covariance relationships to enhance prediction accuracy and accelerate genetic gains.
Speakers
JC

Jessica Chitwood-Brown

University of Florida
Co-authors
LG

Leonard Gaspar

University of Florida IFAS GCREC
NA
SH

Samuel Hutton

University of Florida
NA
Wednesday July 30, 2025 9:00am - 9:15am CDT
Foster 2

9:15am CDT

VGBR 1 - “Seed to Seed in 100 Days”: Development of a Low-Cost Speed Breeding Protocol for Chile Peppers (Capsicum annuum L.)
Wednesday July 30, 2025 9:15am - 9:30am CDT
Speed breeding is a technique that utilizes controlled environments and optimal lighting (photoperiod) conditions to accelerate germination, development, and maturity of plants. One of the major constraints for its adoption and implementation in vegetable breeding programs is the high cost associated with growth chambers. The objective of this study is to develop a Speed Breeding protocol for chile peppers using an improvised, low-cost growth chamber constructed using polyvinyl chloride (PVC) plastic, greenhouse film, mylar reflective film, full-spectrum and far-red light-emitting diode (LED) growth lights. Four C. annuum L. genotypes, namely, NuMex Lotalutein (a serrano type), NuMex Odyssey (New Mexican), NuMex Las Cruces (cayenne), and Early Jalapeno (jalapeno) were planted in two randomized flat trays, using SunGro propagation soil, and watered twice daily. After reaching the 2-3 leaf stage, the treatments (control and Speed Breeding, SB) were transplanted into 8” pots, incorporating sterilized and LM-AP soil mixtures. The control group was cultivated in standard greenhouse conditions for growing chile peppers, exposed to normal daylight intensity and duration, and an average temperature of 21°C and humidity of 40%. The chamber was also constructed in the same greenhouse. From weeks 1 to 5 after planting, the SB-treatment was subjected to 20h/4h light/dark conditions daily, and 9h/15h light/dark after week 5. Light intensity was increased by ~100 photosynthetic active radiation (PAR) weekly after week 5, from ~150 PAR to ~800 PAR. Data was recorded weekly to examine the effects of treatment on germination rate, plant size, leaf number and color, number of flowers, buds, and fruits, and days to flower and fruit. There were significant differences (Tukey HSD, P < 0.05) between treatments for germination rates, number of buds, fruits, leaves, and days to flowering. The SB-treated pepper plants exhibited faster and higher germination, darker green leaves, and a higher number of buds and fruits compared to the control. For instance, SB plants started flowering, on average, about 20 ± 5 days earlier, germinated 5 ± 1 days earlier, and had, on average, 15 ± 2 more buds than the control. Notably, all genotypes under the SB-treatment had at least one fully mature fruit ~106 days after sowing. These results suggest that, at minimal costs, there is a potential to achieve increased generation times to accelerate cultivar development and genetic improvement in chile peppers.
Speakers
SN

Shahab Nourbakhsh

New Mexico State University
Co-authors
AV

Amanda Velazco

New Mexico State University
NA
DN

Dennis Nicuh Lozada

New Mexico State University
UM

Uriel Martinez

New Mexico State University
Wednesday July 30, 2025 9:15am - 9:30am CDT
Foster 2

9:30am CDT

VGBR 1 - Watermelon Glycemic Index Footprint: Management through Production System, Breeding and Precision Dieting
Wednesday July 30, 2025 9:30am - 9:45am CDT
Watermelon is a preferred fruit in the Caribbean and all over the world and is the second most consumed cucurbit by harvest weight in the Virgin Islands after cucumber. While quality of water of watermelon is commonly judged by sweetness, the ratios of types of sugars are hardly considered. Watermelon is classified as one of the fruits with very high glycemic index, GI – the measure by which a dietary intake increases the blood sugar compared to pure glucose whose GI is 100. Watermelon is normally promoted as a highly nutritious as it is one of the best sources of potassium, but can be both a blessing and curse, especially for consumers with high blood sugar-related ailments. Here we report on preliminary efforts we are making to produce watermelons that have reduced GI. We are using hybridization as well as exploiting genotype-by-environment interactions to influence sugar composition in fleshy fruit tissues. We have obtained three hybrids that have at least 22% reduced glucose and at per brix compared to the parents. We are also testing a series of shadehouse systems, two of which show the potential to lower glucose ratio in the fruits. We hope to refine these methods and evaluate the watermelon lines for yield and sugar trait stability before recommending plant materials and production systems to interested farmers. Key words: Hybrids, sugar, glycemic index, genotype by environment, production system, sucrose, watermelon.
Speakers
HA

Henry Awika

University of the Virgin Islands
Co-authors
DG

Donna Gonzalez

University of the Virgin Islands
NA
HH

Hannah Hazard

University of Wisconsin Oshkosh- Oshkosh
NA
JS

Johnson Southwell

University of the Virgin Islands
NA
Wednesday July 30, 2025 9:30am - 9:45am CDT
Foster 2
  Oral presentation, Vegetable Breeding 1

9:59am CDT

ORGH 1 - Oral Session
Wednesday July 30, 2025 9:59am - 10:00am CDT
Presiding/Moderator
SP

Samantha Pratt

Oklahoma State University
Wednesday July 30, 2025 9:59am - 10:00am CDT
Foster 2

10:00am CDT

ORGH 1 - Effect of Microalgae Application on Yield and Nutrient Composition of Arugula
Wednesday July 30, 2025 10:00am - 10:15am CDT
Effect of Microalgae Application on Yield and Nutrient Composition of Arugula Sam Pratt1*, Shivani Kathi1, Steve Phillips2, and Justin Moss1 1Department of Horticulture and Landscape Architecture, Oklahoma State University, Stillwater, OK, 74078 2Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078 *Presenting author- Samatha.pratt@okstate.edu Chlorella (Chlorella vulgaris) has been used as a biofertilizer in horticultural crop production in controlled environment production systems. However, research gaps exist in the application of chlorella biofertilizer in field-based production systems. The goal of this research is to assess the impact of microalgae biofertilizers on crop productivity and nutritional quality of arugula grown in open-field using certified naturally grown production practices. To understand the impact of microalgae biofertilizer on soil nutrient and organic matter content, soil testing was done before and after treatment. Treatments included 50% and 100% nitrogen fertilizer and chlorella applied through foliar, soil and foliar soil application. Chlorella application began a week after transplanting. Data was collected on number of leaves per throughout the study and fresh and dry weight at harvest. Plant samples were analyzed for nutrient composition of leafy greens (chlorophyll, carotenoids, and mineral nutrients composition). Results show that soil applied chlorella was more effective application method regarding the overall impact on yield. Continued research on the addition of Chlorella in crop production can offer additional nitrogen sources for low chemical input and organic based production systems.
Speakers
SP

Samantha Pratt

Oklahoma State University
Wednesday July 30, 2025 10:00am - 10:15am CDT
Foster 2

10:15am CDT

ORGH 1 - Effect of Organic Fertilizer on Broccoli Yield and Soil N Mineralization
Wednesday July 30, 2025 10:15am - 10:30am CDT
Organic vegetable farmers use a wide variety of nitrogen (N) fertilizers from commercial products (made from animal and seed meals) to manures and composts. These organic N sources must undergo mineralization and nitrification to become plant available inorganic N (microbial processes). How much and when nitrogen becomes available can be highly variable dependent on product characteristics and local weather conditions, and this subsequent N availability can greatly impact yield. The objective of this study was to determine nitrogen mineralization, head timing, and overall yield from seven different organic fertilizer treatments on fall broccoli (Brassica oleracea ‘Castle Dome’). Studies were conducted over two years at the UGA Durham Horticulture Farm on certified organic land and organic fertilizers (with the exception of the control) were applied to provide a rate of 134 kg N ha-1. Fertilizers ranged from processed animal meals to composts and fresh poultry litter. Nitrogen mineralization was measured using in-situ soil cores and ranged from 0 (immobilization) to 100% of the N applied. Significant N was observed from the soil only treatment which provided on average 100 kg N ha-1 over the growing season. In both years, overall higher yields were observed with blood meal and lowest yields were observed with compost/control treatments. Fertilizer type impacted the timing of heading and overall harvest windows.
Speakers
KC

Kate Cassity-Duffey

University of Georgia
Co-authors
MC

Miguel Cabrera

University of Georgia
NA
TC

Timothy Coolong

University of Georgia
Wednesday July 30, 2025 10:15am - 10:30am CDT
Foster 2
  Oral presentation, Organic Horticulture 1
  • Subject Organic
  • Funding Source SARE

10:30am CDT

ORGH 1 - Leveraging Rendered Animal and Treated Manure Products For Nutrient Management and Anaerobic Soil Disinfestation in Organic Watermelon Production
Wednesday July 30, 2025 10:30am - 10:45am CDT
Nutrient and weed management are two of the biggest challenges in organic vegetable production systems. Anerobic soil disinfestation (ASD) is an effective method to manage the weeds and soil borne pathogens in organic production systems. In ASD, a carbon source is added to soil followed by watering to saturation and covering with plastic mulch to create anerobic soil conditions. Rendered animal and treated manure products have significant content of plant nutrients and carbon and can serve both as organic fertilizer and carbon source for ASD. A field experiment was conducted using three different carbon sources meat and bone meal (MBM; rendering product), MBM Sulfur and treated manure solids with objective to evaluate the effect of different carbon sources and their application rates on nutrient management and ASD in organic watermelon under traditional and novel charter next generation plastic mulch. We will be evaluating the treatment effect on soil cumulative anaerobicity, percent weed coverage, yellow nutsedge rhizome viability, plant vigor ratings, shoot and root biomass, periodic nutrient leaching, soil nitrogen and phosphorus content, plant tissue nutrient content (root and shoot), soil pH, soil C, N and P mineralization enzymes and soil microbial biomass. In results, MBM with sulfur at 150% recorded the highest cumulative anaerobicity and low weed coverage suggesting that it could be an excellent tool for soil nutrient management and weed control in organic production system.
Speakers
avatar for Ashok Sah

Ashok Sah

Clemson University
I am a Ph.D. student working on anaerobic soil disinfestation (ASD) technique, its application for nutrient and weed management in horticultural crops (watermelon).
Co-authors
AT

Anastasia Thayer

Clemson University
NA
BJ

Bhupinder Jatana

Clemson University
BW

Brian Ward

Clemson University CREC
Wednesday July 30, 2025 10:30am - 10:45am CDT
Foster 2
  Oral presentation, Organic Horticulture 1
  • Subject Organic
  • Funding Source USDA

10:45am CDT

ORGH 1 - Short-Term Effects of Organic Cover Crops on Macronutrient Cycling and Soil Properties
Wednesday July 30, 2025 10:45am - 11:00am CDT
This study presents the evaluation of ten organic cover crops on soil fertility and macronutrient accumulation within their biomass. Field trials were conducted at the organic farm of Tennessee State University using a randomized complete block design with four replications under National Organic Program (NOP) management practices. The cover crops assessed includes barley, crimson clover, daikon radish, field peas, hairy vetch, mammoth red clover, medium red clover, tillage radish, white clover, and winter rye. Each plot measured 16 ft x 7 ft within a whole plot size of 190 ft x 63 ft. Cover crops were sown using a seed drill, adhering to USDA and SARE recommended seed rates. Soil Samples were collected at a foot depth both before and after cover crops termination to analyze the variations in soil properties such as pH, phosphorus, potassium, calcium, magnesium, zinc, iron, manganese, boron, sodium, and organic matter. Aboveground biomass was clipped from 1ft2 quadrants across all replications, dried at 60oC for 72 hours. Samples were grinded to a fine powder using a 1100-watt Ninja blender and analyzed for nitrogen and carbon content using Near-Infrared Spectroscopy (NIRS). Results indicated that most of the cover crops increased soil organic matter, phosphorus, sodium, magnesium, and iron. In contrast, declines were observed in soil pH, potassium, manganese, calcium, boron, zinc. Significant differences were observed in the nitrogen and carbon accumulation among cover crops. Hairy vetch showed the highest nitrogen (4.24%) and carbon (46.62%) content, while barley and daikon radish had the lowest nitrogen (1.55%) and carbon (40.66%) content, respectively. The carbon to nitrogen (C:N) ratio ranged from 11:1 to 30.1:1, with legumes generally exhibiting lower ratios (11:1 to 15.3:1) demonstrating higher decomposition and quicker nutrient release. In contrast non-legumes had the higher ratios (25.4:1 to 30.1:1), suggesting slower nutrient release. These findings highlight the short-term impacts of different organic cover crops on soil fertility and nutrient cycling. The variation in macronutrient accumulation and C:N ratios provides practical insights for the growers in selecting appropriate cover crops to enhance soil health and support sustainable agricultural systems.
Speakers
SP

Sai Prakash Naroju

Tennessee State University
Co-authors
DN

Dilip Nandwani

Tennessee State University
NA
SC

Shahidullah Chowdhury

Tennessee State University
NA
Wednesday July 30, 2025 10:45am - 11:00am CDT
Foster 2
  Oral presentation, Organic Horticulture 1
  • Subject Organic
  • Funding Source TSU Co-Operative Extension

11:00am CDT

ORGH 1 - Biostimulants to complement organic fertilizers in organic transplant production
Wednesday July 30, 2025 11:00am - 11:15am CDT
The increasing demand for sustainable agricultural practices has driven the adoption of organic farming and increased demand for organic transplants. However, transplant growth is often lower in organic fertilizers compared to conventional fertilizers. This is most likely caused by low nutrient availability in organic fertilizers. On the other hand, increasing organic application rates has the potential to induce salinity stress/toxicity. We hypothesize that biostimulants can complement organic fertilizers in crop production by enhancing nutrient uptake and increasing crop tolerance to abiotic stress and thus close the yield gap between organic and conventional fertilizers. In this study, we tested three biostimulants: humic acid and molasses (MicroLife Soil and Plant Energy) and two microbial based biostimulants with different beneficial microorganism populations (MicroGrow CM and MicroGrow Supreme). Four organic fertilizers were tested with biostimulants, two of which were animal-based fertilizers: Sustane (4-6-4, turkey manure) and Drammatic (2-4-1, derived from fish scrap) and two of which were plant-based fertilizers: Nature Safe (7-7-7, corn steep liquor) and Pre-Empt (fermented sugarcane molasses). After applying to peat-based substrate, we analyzed the leachate nutrient availability weekly. Both fertilizer and biostimulant affected available nutrient concentrations in substrate. Additionally, transplant performance was evaluated to determine the effect of biostimulant and organic fertilizer combination on transplant growth, mineral nutrition, and physiological responses. Transplant growth varied across the different combinations of biostimulants and organic fertilizers tested, suggesting potential interactions between these factors. Some biostimulant treatments, particularly in combination with specific organic fertilizers, showed a trend towards improved early growth compared to the organic fertilizers alone. These results showed synergistical effects between biostimulants and organic fertilizers, indicating the positive role of biostimulants in organic transplant production.
Speakers
JL

Jun Liu

Texas A
Co-authors
Wednesday July 30, 2025 11:00am - 11:15am CDT
Foster 2
  Oral presentation, Organic Horticulture 1
  • Subject Organic
  • Funding Source Specialty Crop Multi-State Program grant TX-SCM-21-05, Hatch Project TEX07726, and Specialty Crop Block Grant GSC2022030.

11:15am CDT

ORGH 1 - Long-term Application of Animal and Forest Waste on a Vineyard and its Effect on Yield and Soil Health
Wednesday July 30, 2025 11:15am - 11:30am CDT
Increased concerns over the last several decades on environmental quality have stimulated farmers to accept organic farming as an alternative to inorganic agriculture. Muscadine (Vitis rotundifolia var. Summit) is native to the southeastern United States and has been cultivated for over 400 years. Muscadine and its products are highly nutritious and a good source of antioxidants and dietary fiber that have positive effects on health. Muscadine was grown on Memphis Silt Loam soil (Typic Hapludalph, silty, mixed, thermic). Three treatments of organic manures (cow-C; poultry-P; cow poultry-CP) with gypsum and pine mulch were applied in a CRD. Control received inorganic fertilizers and traditional cultural practices. Dolomitic lime was applied every 2 to 3 years to balance the soil pH of the soil and the pine needle was continuously applied as a thick mulch to bring down the soil pH to neutral and also to prevent moisture evaporation from basins where fresh animal waste was applied. This thick layer of pine needle mulch helped avoid irrigation even during the hottest summer. While strictly following the protocol, soil samples were collected and analyzed at Cornell Soil Health Lab, Cornell University, Ithaca, NY, after 17 years of continuous organic research with the same treatments. The experimental design for data analysis was a completely randomized design with three replications for each treatment. Leaf area index (LAI), percent canopy cover, stem diameter, and yield were higher in organic plants. There was no significant difference in diameter, length, and degree brix of the fruit. Soil compaction was always higher in control with lower soil moisture content and the compaction was lower in organic treatments due to higher level of organic matter content. Concentrations of nitrate-N and P were higher in the surface soil treated with organic manures, but there was no trend in N or P enrichment in the lower layers of the soil. A comprehensive assessment of soil health done by Cornell Soil Health Lab showed the following: physical aggregate stability 19.0 25, biological organic matter 4.0 85, biological ACE soil protein index 6.8 43, biological soil respiration 0.8 75, biological active carbon 747 90 and chemical soil pH 7.1 100 with an overall quality score of 72 (Excellent). The results suggest that the controlled application of animal and forest wastes in basins of fruit trees can be an agronomically and environmentally sound practice to increase yield and keep the soil and humans healthy.
Speakers
avatar for Girish Panicker

Girish Panicker

Professor of Plant and Soil Sciences & Director of Conservation Research, Alcorn State University
Dr. Girish  Kumar Panicker, Professor of Plant and Soil Sciences and Director of Conservation Research, at Alcorn State University, Mississippi, is an authority on cover and management (C-factor) research on horticultural crops and is an internationally renowned agricultural scientist... Read More →
Co-authors
WM

Willie Mims

Alcorn State University
NA
Wednesday July 30, 2025 11:15am - 11:30am CDT
Foster 2
  Oral presentation, Organic Horticulture 1
  • Subject Organic
  • Funding Source USDA/NIFA

11:44am CDT

Undergraduate Student Oral Competition - 1
Wednesday July 30, 2025 11:44am - 11:45am CDT
Wednesday July 30, 2025 11:44am - 11:45am CDT
Foster 2

11:45am CDT

UG 1 - Is ‘Arbequina’ Still the Best Option for California? An Evaluation of Established and Novel Varietals in a Super High Density Hedgerow System in the Sacramento Valley
Wednesday July 30, 2025 11:45am - 12:00pm CDT
Global olive oil production tripled over the past six decades to 2.76 million tons in the 2022/23 year. California is the largest producer of olive oil in the United States, and its planting is increasing at an estimated rate of 3000 acres per year. Most of the new plantings for oil in California are in hedgerows under intensive management either in a super high (SHD) or medium (MD) density systems. Of these plantings, an estimated ~80% is ‘Arbequina’, ~15% ‘Arbosana’, and ~5% ‘Koroneiki’. As expected, the industry is continuously searching for novel cultivars that can improve productivity, as well as extra virgin olive oil (EVOO) quality, thereby increasing  returns for their operation. With industry support, a trial was planted at the Wolfskill Experimental Orchard in Winters, CA in 2019 with the exception of ‘Lecciana’ which was planted in 2020. Four acres were planted at a SHD rate of of 620 trees per acre, with 5 feet between trees and 14 feet between rows. ‘Arbequina’, ‘Arbosana’, and ‘Koroneiki’ were planted alongside two new cultivars, ‘Lecciana’ (Olea europaea ‘Arbosana’ x ‘Leccino’) and ‘Sikitita’ (Olea europaea ‘Arbequina’ x ‘Picual’), and one numbered selection, Agromillora 9805-01. The orchard was planted with five complete replicates of the six cultivars, each cultivar row was randomized within each replicate. Seasonal and cumulative yield were measured from the 2021 through the 2024 growing seasons. Fruit weight, oil content, basal shoot prevalence, and maturity index were measured over the 2023 and 2024 growing seasons. The entire orchard was managed following industry agronomic practices for all replicates. During the four years ‘Koroneiki’ had the greatest cumulative yield on average, followed by ‘Arbequina’ and ‘Arbosana’, ‘Lecciana’, ‘9805-01’, and ‘Sikitita’ (16.60, 13.38, 13.37, 10.61, 8.17 and 7.76 tons per acre respectively). Alternate bearing behavior was more prevalent for ‘9805-01’ and ‘Sikitita’. Further, ‘Sikitita’ took an additional year to come into production. These results, alongside seasonal yield, oil content, basal shoot prevalence, and fruit weight, show why ‘Arbequina’, ‘Arbosana’, and ‘Koroneiki’ are the prevalent cultivars in California SHD plantings. In our trial, ‘Arbosana’ and ‘Koroneiki’ performed as well or better than ‘Arbequina’, but California growers have planted less of those two cultivars due to concerns with disease resistance and/or vigor management over time across California’s climatic conditions. Our research shows that further evaluation of novel cultivars is essential for a growing industry.
Speakers Co-authors
AA

Adele Amico Roxas

College Of Agricultural And Environmental Sciences-Olive Center, University of California, Davis
JF

Javier Fernandez-Salvador

College Of Agricultural And Environmental Sciences-Olive Center, University of California, Davis
RJ

Raeva Johnson

College Of Agricultural And Environmental Sciences, University Of California, Davis
Wednesday July 30, 2025 11:45am - 12:00pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1

12:00pm CDT

UG 1 - No Evidence for Polystyrene Nanoplastic Uptake by Lettuce Grown in Nutrient Film Technique Hydroponics Systems
Wednesday July 30, 2025 12:00pm - 12:15pm CDT
As nanoplastics become an increasing environmental and human health concern, greater understanding of their absorption by crop plants is vital. Many plant species, including lettuce, have been reported to transport nanoplastics from roots to leaves, but most research has not been conducted with crops grown in commercial production systems to harvestable size. Across two greenhouse experiments, we compared ‘Winter Density’ romaine lettuce grown with and without the addition of 10 mg.liter-1 yellow-green fluorescent-labeled 0.2 µm and 0.05 µm polystyrene nanoplastics (PSNP) added to municipal irrigation water in commercial-style hydroponic Nutrient Film Technique (NFT) systems. No difference was observed between lettuce grown with or without PSNPs based on plant width; shoot and root fresh and dry weights; number of true leaves; or chlorophyll content. Stomatal resistance of plants in both treatments was also not different and indicated very high transpiration rates that would support PSNP translocation to shoots. Fluorescence of shoots and roots from each treatment was evaluated via confocal microscopy. No PSNPs were found in the shoot tissue. In the roots, we observed PSNPs aggregated on the surface and possibly penetrated a maximum of 10 to 25 microns deep within outer root tissue. In root samples processed with acetone washes, no PSNPs were observed on or in root tissue by either TEM or confocal microscopy. As such, we found no evidence that 0.2 µm or 0.05 µm PSNPs are absorbed into the vascular system of hydroponically-grown lettuce plants and thus are not translocated to shoot tissue. These findings emphasize problems with current literature and the need for further research to investigate PSNP uptake by plants in realistic production systems.
Speakers
AC

Allison Cloyd

Kansas State University
NA
Co-authors
DB

Daniel Boyle

Kansas State University
NA
KW

Kimberly Williams

Kansas State University
MK

MB Kirkham

Kansas State University
NA
Wednesday July 30, 2025 12:00pm - 12:15pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1
  • Poster # csv
  • Funding Source USDA NIFA Hatch-Multistate project 7005973

12:15pm CDT

UG 1 - Growth and Physiological Responses of ‘Little Gem’ and ‘Salad Bowl’ (Lactuca sativa) Lettuce Varieties to Cut-and-Come-Again and Cut-and-Sow Harvest Techniques
Wednesday July 30, 2025 12:15pm - 12:30pm CDT
The "cut-and-come-again" (P
Speakers
BD

Brianna Drew

University of Wyoming
Co-authors
DC

David Claypool

University of Wyoming
EM

Elizabeth Moore

University of Wyoming
NA
GM

Gioia Massa

NASA Kennedy Space Center
NA
JC

Ji-Jhong Chen

University of Wyoming
JK

Jill Keith

University of Wyoming
NA
NA

Nintya Avriantari

University of Wyoming
SM

Shawna McBride

University of Wyoming
NA
Wednesday July 30, 2025 12:15pm - 12:30pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1
  • Poster # csv
  • Funding Source Wyoming Space Grant Consortium Undergraduate Student Fellowship (Grant#: 1004680A) and Faculty Research Initiation Grant (Grant#: 1004680B), NASA EPSCoR Rapid Response Research (R3) Grant#: WY-80NSSC24M0162, University of Wyoming College of Agriculture, Life Sciences, & Natural Resources, Office of Research and Economic Development Designated, Department of Plant Sciences. United States Department of Agriculture National Institute of Food and Agriculture (NIFA) Plant Sciences Hatch Funds (WYO-631-22)

12:30pm CDT

UG 1 - Investigating Silicon Supplementation for Lettuce Grown with Food Waste-Based Anaerobic Digestate
Wednesday July 30, 2025 12:30pm - 12:45pm CDT
Food waste liquid anaerobic digestate (FWLAD) contains higher salt concentrations than synthetic fertilizers at comparable nutrient levels. As a result, plants may experience salt stress at optimal nutrient concentrations or nutrient deficiencies when electrical conductivity (EC) is reduced. Silicon (Si) has been shown to alleviate salt stress and promote plant growth under nutrient-limited conditions. This study investigated whether Si supplementation can mitigate salt stress under high EC and enhance growth under low EC when FWLAD is used to fertilize lettuce (Lactuca sativa ‘Muir’) transplants. Lettuce seeds were sown in either rockwool plugs or a 128-cell plug tray filled with a peat-based growing medium. One week after sowing, seedlings were sub-irrigated with one of nine nutrient solutions prepared from FWLAD. These solutions were adjusted to one of three target EC levels (1, 2, or 3 dS·m⁻¹) and amended with one of three Si treatments: (1) no Si (control, supplemented with 1.5 mM potassium sulfate to match potassium levels), (2) 1.5 mM lab-grade potassium silicate (prepared from silicic acid and potassium hydroxide), or (3) 1.5 mM commercial-grade potassium silicate (AGSIL16H, a commercially available product). Seedlings were grown indoors at 22 °C, with a photosynthetic photon flux density of 200 µmol·m⁻²·s⁻¹ and an 18-hour photoperiod, for a total of three weeks after sowing. For the control treatment without Si, leaf number, total leaf area, and shoot fresh mass were highest at EC 2, indicating that moderate EC promoted optimal growth. Compared to the control, supplementation with lab-grade potassium silicate increased leaf number, total leaf area, and shoot fresh mass by 16%, 63%, and 56% at EC 2, and by 69%, 423%, and 255% at EC 3 in rockwool, and by 10%, 10%, and 11% at EC 2, and by 19%, 42%, and 63% at EC 3 in growing medium. At EC 1, lab-grade potassium silicate had little to no effect on leaf area and shoot fresh mass in either substrate. Commercial-grade potassium silicate did not affect leaf number, leaf area, or shoot fresh mass at any EC. These results suggest that Si supplementation using lab-grade potassium silicate can improve lettuce seedling growth under high-EC conditions when fertilized with FWLAD, with more pronounced effects observed in rockwool compared to the growing medium.
Speakers
TM

Trevor Maxwell

Arizona State University
Co-authors
EF

Estevan Felix

Arizona State University
NA
JB

Jennifer Boldt

United States Department of Agriculture
YP

Yujin Park

Arizona State University
Wednesday July 30, 2025 12:30pm - 12:45pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1
  • Poster # csv
  • Funding Source Foundation for Food & Agriculture Research

12:45pm CDT

UG 1 - Carbon Dioxide Enrichment Increases Yield Without Negatively Affecting Fruit Quality of Everbearing Strawberries Produce
Wednesday July 30, 2025 12:45pm - 1:00pm CDT
Strawberries are the top consumed and produced berry in the United States. Demand for local and off-season availability has increased the production of strawberries in controlled environment agriculture (CEA). While production protocols exist for many leafy greens, limited information is available for emerging CEA crops such as strawberry. Optimizing carbon dioxide (CO2) concentrations for CEA strawberry production is of interest, given that previous research has shown increased yields and enhanced organoleptic properties of strawberries with CO2 enrichment. However, strawberry chemical composition varies depending on cultural practices, environmental conditions, and cultivar. Therefore, the objective of this research was to evaluate the impacts of CO2 enrichment on yield and fruit quality of everbearing strawberries in a controlled environment. Two strawberry cultivars (Fragaria ×ananassa ‘Albion’ and ‘Ozark Beauty’) were propagated from vegetative runners and grown in 2.7-L pots for eight weeks in walk-in growth chambers under either an ambient (450 μmol·mol–1) or enriched (900 μmol·mol–1) CO2 concentration. Temperature and relative humidity setpoints were 20/12 °C and 55/65% (day/night), respectively, with a photosynthetic photon flux density target of 348 µmol∙m–2∙s–1 (18-h photoperiod; daily light integral of 21 mol∙m–2∙d–1). Fruit mass, width, and count were collected weekly on all ripe berries and a USDA grade was assigned based on width and presence of damage. During the final harvest, fruit firmness and Brix:acid were measured to further assess fruit quality and samples were collected for a sensory evaluation. Total yield (grams/plant) of both cultivars was highest under CO2 enrichment. Specifically, total yield increased by 22% and 37% under CO2 enrichment compared to ambient for ‘Albion’ and ‘Ozark Beauty’, respectively. No quality differences (e.g., fruit firmness and Brix:acid) were observed between CO2 concentration treatments for either cultivar. These results were similar to sensory evaluations for rate-all-that-apply (RATA) and affective (9-point hedonic scale) tests (n=84), with little difference observed between CO2 concentration treatments for either cultivar. Participants rated ‘Albion’ higher in overall appearance and overall liking compared to ‘Ozark Beauty’, regardless of CO2 treatment. ‘Ozark Beauty’ consistently rated higher in fermented flavor. In summary, CO2 enrichment increased everbearing strawberry fruit production without negatively impacting berry flavor for these two cultivars. Outreach efforts have sought to disseminate these results and, through program evaluation, identify interest and potential barriers to crop diversification and adoption of CEA strawberry production in Denver, CO.
Speakers
YR

Yamilex Romero

Colorado State University
NA
Co-authors
GS

Gabriel Sanchez

Colorado State University
NA
JH

Jake Holley

Colorado State University
NA
JB

Jennifer Boldt

United States Department of Agriculture
JC

Joshua Craver

Colorado State University
KH

Kale Harbick

United States Department of Agriculture
NA
MC

Martha Calvert

Colorado State University
NA
SP

Samantha P. Rosado

Colorado State University
NA
Wednesday July 30, 2025 12:45pm - 1:00pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1
  • Poster # csv
  • Funding Source USDA-NIFA Project #2023-38640-39571 through the WSARE program under Project #GW24-001

1:00pm CDT

UG 1 - Unravelling the rootstock-scion interaction in grafted watermelon plants
Wednesday July 30, 2025 1:00pm - 1:15pm CDT
As the adoption of grafting technology is increasing among growers to manage soilborne diseases in watermelon production and new rootstocks are being developed, more research is needed to test different combinations of rootstocks and scions to identify growth differences that may be influenced by rootstock-scion interactions. In this greenhouse pot study, we examined the impact of the interspecific hybrid squash rootstock ‘Camelforce’ (Cucurbita maxima × C. moschata) and the wild watermelon rootstock ‘Carolina Strongback’ (Citrullus amarus) on the growth and development performance of two seedless watermelon scion cultivars with contrasting fruit maturity characteristics. Two weeks after seeding, early-maturing ‘Citation’ and late-maturing ‘Miramonte’ seedless watermelon plants were grafted onto each rootstock, respectively, with non-grafted scions and rootstocks as controls. The plants were arranged in a randomized complete block design with five replications and three plants per experimental unit. Non-grafted ‘Camelforce’ showed the longest primary vine, while ‘Miramonte’/‘Camelforce’ displayed significantly higher total vine length than other treatments. Non-grafted ‘Carolina Strongback’ exhibited significantly more leaves, followed by ‘Miramonte’/‘Camelforce’ and non-grafted ‘Miramonte’. ‘Camelforce’ also exhibited significantly greater above and below-ground dry weights, followed by ‘Miramonte’/‘Camel force’, although it did not differ from non-grafted ‘Miramonte’. The dry weights of ‘Citation’/‘Camelforce’ were significantly higher than non-grafted ‘Citation’. The above-ground dry weight of ‘Citation’/‘Carolina Strongback’ was significantly lower than all groups, except for non-grafted ‘Carolina Strongback’. Similarly, the below-ground dry weight of ‘Citation’/‘Carolina Strongback’ was significantly lower than treatments, except for ‘Miramonte’/‘Carolina Strongback’ and non-grafted ‘Carolina Strongback’. All treatments showed male and female flowers by week 3 after transplanting. Both non-grafted rootstocks showed more female flowers than non-grafted and grafted scions. At week 4 ‘Miramonte’/‘Carolina Strongback’ had more female flowers than all other treatments, while non-grafted ‘Camelforce’ exhibited the lowest. No difference in female flowers between the grafted and non-grafted scion groups was observed. Non-grafted scions exhibited more male flowers than the respective grafted plants by week 3. No differences were observed in male flowers except ‘Citation’/‘Carolina Strongback’ with fewer than grafted and non-grafted scions by week 4. Week 3 female flowers results suggest that grafting can significantly affect plant growth, but has a limited impact on flowering. The ‘Miramonte’/ ‘Camelforce’ group exhibited vigorous growth without significantly affecting flowering. Further research is necessary to explore how different types of rootstocks may impact fruit development of watermelon scions of varying fruit development characteristics in commercial production.
Speakers
KL

Kendall Lindsay

University of Florida
NA
Co-authors
XZ

Xin Zhao

University of Florida
Wednesday July 30, 2025 1:00pm - 1:15pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1
  • Poster # csv
  • Funding Source Florida Specialty Crop Block Grant

1:15pm CDT

UG 1 - Accelerated Composting Of Spent Coffee Grounds For Horticultural Soil Amendments
Wednesday July 30, 2025 1:15pm - 1:30pm CDT
Coffee grounds are generated by home residents daily. The objective of this study is to use an accelerated home food scrap composter to encourage residents to reduce waste volume and weight and provide garden compost to produce garden produce and flowers. Two accelerated composters (Lomi and Vego) were evaluated both for their compost soil health, physical and chemical properties after processing the spent coffee grounds. Lomi processed food for 24h only and the Vego composter for 1, 5, and 10 days. Compost volume and weight increased as days of composting increased. Compost health, nutrients, pH, C/N Ratio were suitable for a horticultural growing amendment. Physical components were within normal parameters for a soil substrate. Finer particles were increased with greater days of composting as expected. Plant growth differences were measured. Compost incorporated at 10 % v/v increased petunia biomass. Accelerated composters reduced volume and weight of food scrapes while providing essential plant nutrients.
Speakers Co-authors
AH

Alessandro Holzapfel

Louisiana State University Agricultural Center
EB

Edward Bush

LSU AgCenter
WD

William Dunford

Louisiana State University
NA
Wednesday July 30, 2025 1:15pm - 1:30pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1
  • Poster # csv
  • Funding Option SCRI funded all or part of the research associated with this abstract

1:30pm CDT

UG 1 - Limonoid glucoside and dietary fiber co-treatment enhances salt tolerance, growth kinetics, antioxidant potential, and amino acid metabolism of Lactobacillus plantarum
Wednesday July 30, 2025 1:30pm - 1:45pm CDT
The probiotic strain Lactobacillus plantarum ATCC 8014 is an ideal model for studying prebiotic interactions primarily due to its demonstrated ability to metabolize a wide range of prebiotic compounds. Hence, this study examines the potential synergistic effects of limonoid glucoside (LG), a citrus-derived phytochemical, in combination with dietary fibers on growth kinetics, amino acid metabolism, biofilm formation, and bacterial performance under salt stress conditions. Treatments included de Man, Rogosa, and Sharpe Agar (MRS) media supplemented with various individual and combinations of LG and dietary fibers, pectin (PEC), and inulin (IN). Individual treatments contained 1 mg/mL of LG, PEC, and IN, while combination treatments used a 0.5 mg/mL concentration of each component. Among the tested NaCl salt concentrations of 1%, 2%, and 4%, concentrations below 2% showed sustained bacterial growth for all the combinations (LG: PEC/IN). Salt concentrations at 4 % negatively impacted the bacterial growth in all the treatments and control; however, an exponential rise in growth was observed after the 18th hour of incubation in PEC: LG, indicating a delayed lag phase. Such a pattern of growth at high salt stress possibly indicates that the co-treatment with LG and PEC could potentially maintain growth, help conserve energy demands, and display osmoregulatory effects to overcome salt stress. Additionally, differences in cell surface hydrophobicity between treatments suggest improved bacterial adhesion and biofilm formation. The LG treatment resulted in significantly increased hydrophobicity, indicating enhanced adhesion potential. IN, LG: PEC/IN treatments showed moderate hydrophobicity, comparable to the MRS control, while PEC exhibited the least. These findings are important, as higher cell surface hydrophobicity is crucial for providing protection against environmental stressors and supporting healthy microbial interactions. Amino acid profiling showed treatment-dependent changes, suggesting that LG and PEC/IN combinations affect amino acid metabolism significantly. Additionally, digested samples exhibited enhanced radical scavenging, indicating potential antioxidative synergy between digested LG and dietary fibers. By analyzing the effects of limonoid glucosides in combination with dietary fibers, this study highlights the potential synergistic benefits of these compounds in enhancing their functional properties and supporting a healthy gut microbiome.
Speakers
AS

Ananya Sharma

Texas A&M University
Co-authors
BP

Bhimanagouda Patil

Vegetable and Fruit Improvement Center, Texas A&M University
NA
DK

Deepak Kumar Jha

Texas A&M AgriLife
VD

Vikas Dadwal

Texas A&M University
I am experienced in phytochemical extraction and LC-MS metabolomics of pre and post-harvested fruits and vegetables. My research focuses on the development of plant-derived bioactive formulations and the assessment of their biological activity in vitro and in vivo. I am currently... Read More →
Wednesday July 30, 2025 1:30pm - 1:45pm CDT
Foster 2
  Oral presentation, Undergraduate Student 1

1:59pm CDT

WUM 2 - Oral Session
Wednesday July 30, 2025 1:59pm - 2:00pm CDT
Presiding/Moderator
Wednesday July 30, 2025 1:59pm - 2:00pm CDT
Foster 2

2:00pm CDT

WUM 2 - Assessing Drip Irrigation as an Alternative to Micro-sprinklers During Strawberry Establishment in Southern California – 2nd Season
Wednesday July 30, 2025 2:00pm - 2:15pm CDT
While approximately 80 to 90% of the sprinkler-applied water to a strawberry crop is lost through runoff, deep percolation and evaporation, all the strawberry fields in Ventura County are still irrigated with overhead sprinklers during crop establishment. Even though water use-efficiency for the in-season irrigation is on average high, the escalating regulatory pressure to achieve sustainable groundwater use in California, and therefore limiting water availability, will demand further efficiency. This study quantified differences in water use, yield, canopy coverage and root depth between drip tape (DT) and micro-sprinkler (MS) irrigation during crop establishment at a commercial field located in Oxnard, CA during the 2023-2024 and 2024-2025 growing seasons. This abstract shares the results of the second season. The treatments were applied during the first 42 days after planting, after which drip tape became the only irrigation method. The irrigation scheduling of the MS treatment was defined by the irrigator (grower standard), while the DT irrigation was guided by tensiometers and field observations. All other production practices remained the same. Each treatment was replicated four times in a randomized complete block design, with an area of 0.15 acre per plot (7 beds of 175ft long). Total water use during establishment was 74.4% greater for the MS treatment compared to DT (5.8 and 3.4 acre-in, respectively). Marketable yield up to March 31 was very similar between treatments (19,268 and 19,420 lb/acre for DT and MS, respectively) and not statistically significant (p-value = 0.9517). Although not statistically significant, canopy cover MS was 9 and 11% greater at 1 month and 3 months after planting, respectively. Root depth measured at 1 month after planting was very similar and not statistically different (p-value = 0.9496).
Speakers Co-authors
GF

Gina Ferrari

UC Cooperative Extension
NA
OD

Oleg Daugovish

University of California Cooperative Extension
RC

Raul Coronado

Good Farms
NA
Wednesday July 30, 2025 2:00pm - 2:15pm CDT
Foster 2

2:15pm CDT

WUM 2 - Evaluating OpenET against field scale surface renewal ET in walnuts
Wednesday July 30, 2025 2:15pm - 2:30pm CDT
Walnut is currently grown on over 400 thousand acres in California with the majority of production in the Northern San Joaquin Valley (NSJV). The recurring droughts and climate change in California will likely increase the uncertainty in water supply to walnuts and other specialty crops. Site-specific irrigation is critical to cope with these challenges. Knowing the water use of walnuts is crucial in optimizing irrigation management since it affects nut quality, and productivity. Unlike traditional methods, which are often limited by spatial coverage, high costs, and less precise crop coefficient values, satellite remote sensing offers a cost-effective, widely accessible solution. It enables large-scale evapotranspiration (ET) estimation with increasing adoption in irrigated agriculture, providing a valuable tool for water management. This study compares OpenET models, an open-source database providing ET estimates, against commercial in-situ surface renewal ET sensor. Utilizing OpenET platform provides a good opportunity for growers to improve water use efficiency. Such improvements could lead to the adoption of publicly available irrigation management tools and ensure healthier tree development, better resource utilization, and more resilient orchards in the face of climate change. Based on the data of 2024 season, the Satellite Irrigation Management Support (SIMS) model had the highest accuracy in estimating actual ET when compared to measurements from a commercial in-situ surface renewal system in the orchard, with a mean percent error (MPE) of -18.45%, and R² and mean absolute error (MAE) values of 0.88 and 0.03 inches/d⁻¹, respectively, followed by the Ensemble model. In contrast, the SSEBop model showed the lowest correlation with ETa, with an R² of 0.77 and a relatively high MAE of 0.06 inches, indicating a higher level of uncertainty in its estimates which could potentially lead to over-irrigation if adopted without correction. Based on these findings, growers can confidently incorporate the OpenET SIMS model into their irrigation scheduling, ultimately enhancing water use efficiency. However, further validation through replication over a second year and across multiple sites is essential to substantiate these findings.
Speakers
AM

Abdelmoneim Mohamed

Irrigation and Soils Advisor, University of California ANR
Wednesday July 30, 2025 2:15pm - 2:30pm CDT
Foster 2

2:30pm CDT

WUM 2 - Evaluation of the Effects of Treated Wastewater on the Growth of Rose-Scented Geranium and English Lavender
Wednesday July 30, 2025 2:30pm - 2:45pm CDT
Water scarcity is one of the major challenges facing the agricultural industry, necessitating the use of treated wastewater for irrigation purposes. However, not all crops can effectively utilize this water, as it may have negative effects on plant growth, including disruptions in nutrient uptake and photosynthesis. This study aimed to evaluate the effects of treated wastewater on the growth of rose-scented geranium (Pelargonium graveolens) and English lavender (Lavandula angustifolia). The experiment was conducted between 2022 and 2023 at the University of Fort Hare, Dikeni, South Africa. Two harvests were carried out in May 2023 (Harvest 1) and October 2023 (Harvest 2). Five irrigation treatments were applied to both geranium and lavender plants, consisting of treated wastewater from Dikeni town mixed with tap water at varying proportions: 0%, 25%, 50%, 75%, and 100% (v/v). Water and soil used were tested for nutritional composition. Treatments commenced four weeks after transplanting from cuttings, and the experiment followed a completely randomized block design with four replications across four blocks. The results showed no treatment differences in stem diameter, number of shoots, or plant height across treatments for both plant species during the two harvesting seasons. However, English lavender plants irrigated with 25% wastewater exhibited a slight increase in plant height at week 11, while geranium plants treated with 75% wastewater showed an increased plant height from week 5 until harvest at week 11. Additionally, plants receiving the 25% wastewater treatment produced the highest number of shoots from week 8 to week 11. These findings suggest that treated wastewater, both in its diluted and undiluted form, did not adversely affect plant growth. Therefore, it has the potential to serve as an alternative water and nutrients source for geranium and lavender plants, which farmers could utilize in collaboration with local municipalities to mitigate water scarcity challenges. However, further studies, particularly under open-field conditions, are needed to validate these results.
Speakers
SM

Sandile Manzi Ngwenya

University of Fort Hare
Co-authors
BK

Bahlebi Kibreab Eiasu

University of Fort Hare
NA
YT

Yandisa Tokota

University of Fort Hare
NA
Wednesday July 30, 2025 2:30pm - 2:45pm CDT
Foster 2
  Oral presentation, Water Utilization and Management 2

2:45pm CDT

WUM 2 - Evaluating Nutrient Remediation and Growth of Hibiscus moscheutos ‘Luna Pink Swirl’ in Modified Raingarden Medias
Wednesday July 30, 2025 2:45pm - 3:00pm CDT
Runoff containing excess nitrogen (N) and phosphorus (P) is detrimental to environmental and human health. Bioreactors are biological treatment systems that can be used to combat these problems, which often consist of a lined trench filled with a carbon-rich media (often woodchips) to promote biological remediation through denitrification and other processes. Various carbon-rich organic materials, such as woodchips and sugarcane bagasse (a byproduct of sugarcane production), can be used to fuel biological processes, whereas inorganic materials, such as expanded shale, can provide binding sites for P adsorption as well as physical stability within medias. Raingarden installations utilize similar concepts for trapping runoff water and remediating contaminants; however, the inclusion of ornamental plants provides aesthetic appeal, an important consideration in urban and suburban areas. Seven unique medias were evaluated to determine potential to a.) remediate N and P from runoff and b.) support plant life. An aged pine bark/sand media commonly used for landscape beds (bed mix; BM) served as the control. Organic carbon sources (woodchips (WC) and sugarcane bagasse (SB) to support bacterial communities) and several inorganic materials (including expanded shale (S) and activated aluminum (AA) to bind P) were blended with BM to provide potential enhancement of nutrient removal capabilities. Media blends were homogenized by hand before being transferred into media containers (MC; 2.36 L of substrate per container), wherein Hibiscus moscheutos ‘Luna Pink Swirl’ seedlings were transplanted. Pots were placed within plastic containers (leachate containers (LC)), which served as a collection receptacle for leachate. Simulated runoff water containing N and P was prepared and applied to each replicate, after which leachate was analyzed and collected as sub-samples. Simulated runoff applications were limited in the initial phase of the study (one application per week; three total) and intensified in the final phase (three applications per week; nine total), with all leachate volumes collected between applications. Health and growth of Hibiscus was assessed via SPAD readings, growth index, and destructive harvest at the termination of the study. While the growth of all Hibiscus replicates was generally equivalent between treatments, it was observed that BM amended with SB produced more shoot biomass. Additionally, leachate nutrient content and water chemistry dynamics were influenced by several of the investigated amendments.
Speakers
BS

Brianna Slade

Louisiana State University Agricultural Center
NA
Co-authors
DA

Damon Abdi

Louisiana State University Agricultural Center
JF

Jeb Fields

University of Florida
JK

Jeff Kuehny

Louisiana State University Agricultural Center
NA
JB

Jeffrey Beasley

University of North Carolina - Pembroke
NA
JP

John Pardue

Louisiana State University
NA
MH

M.P. Hayes

Louisiana State University
NA
MA

Mysha Ahmed

Louisiana State University
NA
Wednesday July 30, 2025 2:45pm - 3:00pm CDT
Foster 2
  Oral presentation, Water Utilization and Management 2

3:00pm CDT

WUM 2 - Evaluating Colorado Native Plants for Green Infrastructure: Survivability and Performance in Semi-Arid Bioretention and Green Roof Systems
Wednesday July 30, 2025 3:00pm - 3:15pm CDT
Green infrastructures, when implemented, need to consider the specifics of the local area and climate. The semi-arid climate along Colorado’s front range creates a challenge for plants in green infrastructure systems such as bioretention facilities and green roofs. These plants experience inundation and fast infiltration during rain events and long periods of hot and dry conditions between storms. To accommodate these periods of inundation and drought, Colorado native plants were evaluated due to their adaptation to the challenging conditions that occur in green infrastructure. The experiments in this study are intended to build upon existing work performed by our collaborators and funders Mile High Flood District, the City and County of Denver, and Colorado State University. We aim to improve green infrastructure facility design by using new bioretention media mixes, amended native soils with 5% compost (SSC) and amended native soils with biochar and zeolite (SBZ), green roof components/systems, new plant growth and establishment strategies, and lower irrigation regimes. In 2023 and 2024, data on plant survivability were collected from the bioretention facilities and the green roofs using 100 containerized and bare-root plants. The five native Colorado species selected were Agastache rupestris, Liatris ligulistylis, Amorpha canescens, Ratibida pinnata, and Schizachyrium scoparium. L. ligulistylis, in container, had a higher survival rate after one year on the green roof, on the other hand, it had a low survival rate after a year in the bioretention facilities. The bare-root L. ligulistylis had a higher survival rate, especially in the SSC media. A. canescens, bare-root, had a higher survival rate in the SSC media, while only 25% survived in the SBZ media. All other species overwintered and grew larger the second year on the green roof and bioretention facilities. The Colorado native plants tolerated challenging conditions better than the nonnative plants in the surrounding area.
Speakers
AS

Amanda Salerno

Colorado State University
Wednesday July 30, 2025 3:00pm - 3:15pm CDT
Foster 2

3:15pm CDT

WUM 2 - Partitioning Evapotranspiration in a Young Pistachio Orchard Using the Conditional Eddy Covariance (CEC) Method in the Mesilla Valley, NM
Wednesday July 30, 2025 3:15pm - 3:30pm CDT
Understanding the partitioning of evapotranspiration (ET) into soil evaporation and plant transpiration is critical for improving irrigation management in young orchards with limited canopy cover. This study focuses on partitioning ET in a 4-year-old drip irrigated pistachio orchard located in the Mesilla Valley, Southern New Mexico, using the Conditional Eddy Covariance (CEC) method. The orchard is equipped with a high-frequency eddy covariance system along with sensors to make meteorological measurements. The CEC approach was applied to identify and isolate flux contributions under specific atmospheric conditions, thereby separating transpiration-driven and evaporation-driven fluxes. The CEC separates fluxes using conditional sampling based on the hypothesis that when transpiration is dominant, CO₂ and H₂O fluxes should be highly correlated. Preliminary results show that the total ET values measured from June to August varied between 1.5 and 4.5 mm/day, with about 20% contributed through transpiration from the plants in the year 2024.
Speakers
UP

Uduwarage Perera

New Mexico State University
Co-authors
AS

A. Salim Bawazir

New Mexico State University
NA
AF

Alexander Fernald

New Mexico State University
NA
CC

Chris Chavez

New Mexico State University
NA
RH

Richard Heerema

New Mexico State University
NA
Wednesday July 30, 2025 3:15pm - 3:30pm CDT
Foster 2
  Oral presentation, Water Utilization and Management 2

3:30pm CDT

WUM 2 - Seasonal Water Use and Yield Productivity: Comparing Organic vs. Conventional Iceberg Lettuce with Different Irrigation Scheduling Strategies under Surface Drip Irrigation in Yuma, Arizona
Wednesday July 30, 2025 3:30pm - 3:45pm CDT
Lettuce (Lactuca sativa) is a key winter vegetable with significant consumptive water use in the Lower Colorado River Basin, especially in Yuma, AZ. Generally, lettuce requires about 300 – 400 mm to produce a desirable crop, which can vary significantly depending on irrigation method, soil type, field slope, temperatures, and planting window. However, the magnitude of the quantified differences in seasonal evapotranspiration and crop water productivity among different irrigation scheduling strategies under the subsurface drip irrigation method for organic vs. conventional iceberg lettuce production systems has not been sufficiently investigated. Field experiments were conducted in the fall 2024 growing season at the Valley Research Center at the University of Arizona Yuma Agricultural Center, Yuma, Arizona. This project was conducted in a one-acre field (half-acre organic field and the other half conventional field) under the subsurface drip irrigation method with two irrigation scheduling strategies (sensor-based irrigation (SI) and traditional irrigation (TI) based on growers' standard decision basis that is common in the Yuma area. The field was planted with the iceberg lettuce variety SVLD0023 on October 29th, 2024, on Gadsden clay loam soil. The fertilizer treatments imposed included (1) organic fertilizer, (2) combined biostimulant and organic fertilizer in an organic lettuce field, (3) nitrogen, and (4) combined biostimulant and nitrogen in a conventional lettuce field. Each treatment was replicated three times within each experimental block. Each experimental unit had three beds, and each bed was approximately 120 feet long and 3.5 feet wide, with a randomized complete block design. The objectives of this project include: (1) quantify and compare the seasonal iceberg lettuce evapotranspiration between organic and conventional iceberg lettuce production systems, (2) measure and compare the crop water productivity, and irrigation water use efficiency between two irrigation scheduling strategies for organic and conventional iceberg lettuce production systems, and (3) quantify and compare improvements in soil water retention under the combined application of biostimulant and organic fertilizers in organic lettuce versus the combined application of biostimulant and nitrogen in conventional lettuce. Data collection is currently in progress and will be analyzed in a manner consistent with the experimental design and the objectives of the study. Results will be presented with preliminary conclusions and directions for further research.
Speakers
AM

Ali Mohammed

University of Arizona/Yuma Ag Center
Dr. Ali Mohammed is an Assistant Professor and Extension Specialist in the School of Plant Sciences at the University of Arizona, based at the Yuma Agricultural Center. His work focuses on advancing sustainable organic agricultural practices in arid desert regions and holds statewide... Read More →
Wednesday July 30, 2025 3:30pm - 3:45pm CDT
Foster 2

3:59pm CDT

WCPM 2 - Oral Session
Wednesday July 30, 2025 3:59pm - 4:00pm CDT
Presiding/Moderator
CM

Chris Marble

University of Florida/IFAS
Wednesday July 30, 2025 3:59pm - 4:00pm CDT
Foster 2

4:00pm CDT

WCPM 2 - Effect of seed placement on the efficacy of rice hulls for control of 5 common nursery weed species
Wednesday July 30, 2025 4:00pm - 4:15pm CDT
Many types of mulch have been shown to reduce weed growth in container crops. In the past, mulch use in nurseries was not as feasible due to its high cost, but as costs of hand weeding increase, labor shortages become more severe, and environmental awareness rises, utilization of mulch has become more popular in recent years. Parboiled rice hulls are a waste product of the rice industry and are dry rice husks removed from rice grains. They are light weight, hydrophobic and make for an ideal mulch choice in container nursery production and have been previously evaluated for use as a weed management tool in container nurseries. However, research was typically conducted on a limited number of species and studies were performed in greenhouses or other protected structures. The objective of this research was to evaluate rice hulls for control of five common nursery species in an outdoor environment in Florida during the summer season. Nursery pots (1.7 L) were filled with a standard pinebark based potting soil and amended with standard control release fertilizers via incorporation. Upon filling, pots received one of three levels of rice hulls, 0 cm or the non-treated control, 1.3 cm, or 2.5 cm. To test efficacy, approximately 30 seeds of crabgrass (Digitaria sanguinalis), doveweed (Murdannia nudiflora), longstalk Phyllanthus (Phyllanthus tenellus), spotted spurge (Euphorbia maculata), or eclipta (Eclipta prostrata) were sown either above or below the rice hull mulch layers mentioned above. Data collected included weed coverage ratings (0 to 100%) at 4, 6, 8, 10, and 12 weeks after planting (WAP) and shoot dry weights at 12 WAP. Few differences were observed among the different depths of mulch when seeds were placed below. For seeds placed on top of the mulch, the higher 2.5 cm depth generally provided greater control. For all 5 weed species, control significantly increased when seeds were placed on top of the mulch compared to seeds beneath the mulch layer. Control, as measured by a percent reduction in shoot weight compared to the non-mulched control, ranged from 80 to 100% for seeds placed on top compared with decreases of 0 to 40% when seeds were placed below mulch. Overall, data suggest that because rice hulls are more effective on weed seeds placed on top of the mulch layer, growers would likely see increased efficacy when mulching as soon as possible after potting.
Speakers
CM

Chris Marble

University of Florida/IFAS
Co-authors
YK

Yuvraj Khamare

The University of Florida
Wednesday July 30, 2025 4:00pm - 4:15pm CDT
Foster 2
  Oral presentation, Weed Control and Pest Management 2

4:15pm CDT

WCPM 2 - Comparison of Postemergence Herbicide Efficacies Under Elevated and Normal Temperature for Controlling Large Crabgrass in Ornamental Production
Wednesday July 30, 2025 4:15pm - 4:30pm CDT
Elevated temperatures can intensify weed issues by accelerating weed growth and germination, especially for C4 species, due to their heat tolerance and efficient carbon fixations, making their control more challenging. Temperature also influences herbicide absorption, translocation, and metabolism. Large crabgrass (Digitaria sanguinalis), a highly problematic C4 weed, poses a significant threat to nursery and field-grown ornamentals under these conditions. This study aims to evaluate the efficacy of postemergence herbicides at different rates (0.5X, 1X, 2X) under normal and elevated temperatures for managing large crabgrass in ornamental production. Experiments were conducted in summer and fall 2024 using a growth chamber and an open hoop house setup. Large crabgrass seeds were sown in pots filled with standard substrate, irrigated, and placed in separate growth chambers where temperatures were maintained at normal (22–28°C) and at 2-5°C above the species’ optimum range (28-34°C). Once weeds reached the 3–6 leaf stage, they were transferred to an open hoop house for herbicide application. Postemergence herbicides such as topramezone and glyphosate were applied at 0.5X, 1X, and 2X rates using a CO₂ backpack sprayer. After the restricted entry interval, treated weeds were placed back to their respective growth chambers and maintained there for four weeks. A control treatment without herbicide application was maintained in both growth chambers. Weed control ratings were visually assessed at 1, 2, 3, and 4 weeks after treatment (WAT) using a 0–10 scale, where 0 = no control (green foliage, upright growth, no damage) and 10 = complete plant death (dark brown foliage, no green tissue, collapsed structure)). At 4 WAT, all weeds were harvested, and dry weight of aboveground parts of weed was recorded. The experiment followed a completely randomized design with eight replications per treatment and was repeated twice. Data were analyzed using ANOVA in SAS 9.4, and treatment means were separated using Fisher’s LSD test. The results showed that at 4 WAT glyphosate weed control efficacy at normal temperature was 88% which reduced to 73% under elevated temperature condition. Whereas the high temperature increased topramezone efficacy by 10%. The lowest amount of weed dry weight was observed at 2X rates in high temperature conditions, but no difference was observed among rates at normal temperature. Hence it can be concluded that with an increase in temperature, higher rates of the tested herbicides will be required for effective weed management in ornamentals.
Speakers
avatar for Supti Saha Mou

Supti Saha Mou

Michigan State University
Co-authors
DS

Debalina Saha

Michigan State University
Wednesday July 30, 2025 4:15pm - 4:30pm CDT
Foster 2

4:30pm CDT

WCPM 2 - Implementing Anaerobic Soil Disinfestation On-farm for the Management of Soilborne Pests and Pathogens in High Tunnel Production Systems
Wednesday July 30, 2025 4:30pm - 4:45pm CDT
Intensive cultivation in high tunnel production systems is leading to the emergence of soilborne pest and pathogen issues which over time can compromise crop productivity and profitability. Growers facing such challenges are in search of sustainable and effective solutions capable of suppressing soilborne pests and pathogens while preserving soil health. Anaerobic soil disinfestation (ASD) is a broad-spectrum pre-planting biological approach proposed for the management of soilborne issues affecting high-value specialty crops. ASD is applied by incorporating in the soil readily labile organic amendments as a carbon (C) source, tarping the soil with an impermeable film, and irrigating the soil to saturation. Although ASD already proved to be effective against a range of soilborne pests and pathogens across different US regions, crops, and production systems, its adoption at commercial scale remains relatively limited. Factors hindering the adoption of ASD include application cost, complexity of microbial driven processes, and lack of knowledge of the method, its implementation steps, and its efficacy and benefits. Capitalizing on over six years of research aimed at optimizing the ASD application method and its integration in high tunnel production systems in Pennsylvania, a series of on-farm ASD applications were conducted over the 2023 and 2024 growing season with the purpose of demonstrating the technology and let growers evaluate first-hand its viability and efficacy. The on-farm trials were conducted on selected farms growing vegetables and small-berries in high tunnels and employing both conventional and organic production methods. Raised-bed and broadcast application were tested along with the use of clear and black film. Wheat middlings alone or in combination with feed-grade sugarcane molasses were tested as C sources testing different soil incorporation equipment and methods. Depending on the specific crop system, and the grower planting schedule, ASD was applied between the end of the Spring (before a late high tunnel crop planting) and mid-October (after an early high tunnel crop). Parameters measured included soil redox potential, temperature, pH, electrical conductivity and mineral nitrogen. The level of anaerobiosis achieved varied depending on the amount of labile carbon applied, the type of tarp used, and the temperature levels achieved. High levels of cumulative soil redox potential were achieved and the use of clear film allowed to achieve relatively higher soil temperatures and cumulative redox potential levels in most on-farm applications. Additional demonstration efforts are needed to facilitate the adoption of ASD and demonstrate its efficacy.
Speakers
FD

Francesco Di Gioia

Pennsylvania State University
Co-authors
BG

Beth Gugino

Pennsylvania State University
NA
CR

Caterina Roman

Pennsylvania State University
NA
CS

Claudia Schmidt

Pennsylvania State University
NA
ER

Erin Rosskopf

USDA-ARS U.S. Horticultural Research Laboratory
NA
JO

Joe Ono-Raphel

Pennsylvania State University
NA
KD

Kathy Demchak

Pennsylvania State University
NA
LF

Leah Fronk

Penn State Extension
NA
MK

Mihail Kantor

Pennsylvania State University
NA
Wednesday July 30, 2025 4:30pm - 4:45pm CDT
Foster 2
  Oral presentation, Weed Control and Pest Management 2
  • Subject Weed Control and Pest Management
  • Funding Source USDA-NIFA, Organic Research and Extension Initiative Grant # 2021-51300-34914, the USDA NIFA Crop Protection and Pest Management Grant #2021-70006-3531, and the USDA-NIFA Organic Transition Program Grant #2024-51106-43166.

4:45pm CDT

WCPM 2 - Pest Management Using Insect Netting in Organic Chinese Cabbage Production
Wednesday July 30, 2025 4:45pm - 5:00pm CDT
Organic farming with its reliance on natural fertilizers, cover cropping, and crop rotation, presents a sustainable approach to food production. However, a significant challenge in organic production is insect and disease management. Organic insecticides often have limited efficacy, are expensive, and require repeated applications. Mesotunnels, medium-sized (36-40 inches tall) tunnels covered with insect netting, have emerged as a pest management tool for organic vegetable growers. This study evaluated the effectiveness of mesotunnel and low tunnel systems for pest management and season extension in organic Chinese cabbage (cultivar ‘Minuet’) production. The first season of this two-year study was conducted in Fall 2024 on certified organic land at Iowa State University Horticulture Research Station, Ames, IA. The experiment was set up as a randomized complete block design with four replications and following treatments: i) 85 g ExcludeNet insect netting, ii) 0.55 oz Agribon row cover, iii) 85 g ExcludeNet insect netting + OMRI-listed insecticide iv) OMRI-listed insecticide, and v) an untreated control, uncovered with no insecticide. Weekly pest surveillance focused on Brassica insect pests such as aphids, loopers, harlequin bugs, flea beetles, and their damage was recorded. Hobo data loggers recorded variations in light intensity, air and soil temperature, and relative humidity to observe microclimate variations in each treatment. At harvest, yield data was categorized as marketable or nonmarketable and graded according to USDA commercial standards. Among all treatments, the highest marketable produce was recorded in ExcludeNet + OMRI-listed insecticide treatment (42%), followed by Agribon row cover (31%) and the lowest in OMRI-listed insecticide (15%) during the first harvest. The earlier maturity in these treatments was likely due to warmer temperatures and improved humidity regulation under the tunnels. The insect abundance and their damage on leaves was significantly lower in ExcludeNet & ExcludeNet + OMRI-listed insecticide treatment. Integrating netting and row covers with limited use of organic insecticides provided the highest pest suppression, suggesting that physical barriers can effectively optimize pest management and enhance marketability in organic vegetable production systems. Findings from this study will inform growers and the research community about the feasibility of insect nettings as a sustainable pest management tool in organic specialty crop systems.
Speakers
SC

Smriti Chaudhary

Iowa State University
Co-authors
AN

Ajay Nair

Iowa State University
NA
Wednesday July 30, 2025 4:45pm - 5:00pm CDT
Foster 2

5:00pm CDT

WCPM 2 - Do Bicultures of Sunn Hemp with Sorghum Sudangrass Decrease Its Weed and Plant-Parasitic Nematode Suppression Efficacy?
Wednesday July 30, 2025 5:00pm - 5:15pm CDT
Sunn hemp (SH, Crotalaria juncea L.) is used in Florida strawberry production as an off-season summer legume cover crop to provide agroecosystem services such as weed and plant-parasitic nematode (PPN) suppression. Bicultures of SH with sorghum-sudangrass (SS) (Sorghum bicolor Moench × S. sudanense [Piper] Stapf) are of interest to reduce the cost of SH use. Our study objective was to determine whether bicultures retain the weed and PPN suppression benefits provided by SH grown in monoculture. A replacement series experiment was conducted in summer 2022 and repeated in 2023 in north-central Florida in a PPN-infested field. A factorial treatment arrangement of five SH:SS biculture proportions (100:0, 75:25, 50:50, 25:75, and 0:100) and three seeding rates (20, 40, and 60 lb/acre) were evaluated in a randomized complete block design with four replications. A no-cover crop, weedy control was also included in each block. Data were collected on cover crop biomass and carbon: nitrogen ratio (C:N), weed density, weed biomass, and PPN populations at eight weeks after planting. Bicultures consistently produced higher total cover crop biomass than the SH monoculture. Bicultures were as effective as the SH monoculture in reducing total weed density and resulted in either lower or equivalent total weed biomass. Averaged over year, only the 60 lb/acre seeding rate had a lower total weed density than the 20 lb/acre rate. In 2022, both the 40 and 60 lb/acre seeding rates resulted in lower weed biomass than the 20 lb/acre rate, while seeding rate had no significant effect on total weed biomass in 2023. Root-knot nematode populations in both years and sting nematode populations in 2022 were detected at levels too low for analysis. However, in 2023, the lowest and highest sting nematode populations occurred with the SH and SS monocultures, respectively; and sting nematode populations increased as the proportion of SS in the bicultures increased. Bicultures with ≤50% SS had significantly higher sting nematode populations than the weedy control. An increase in C:N was observed as SS proportion in bicultures increased. Thus, we conclude that SH/SS bicultures maintain the weed biomass suppression benefits of SH monocultures while increasing cover crop biomass. However, bicultures may lead to higher sting nematode populations, which will be of concern in organic strawberry production where soil fumigation is not permitted. Growers will also need to consider the effects of the higher C:N ratio of biculture residue on nitrogen fertilizer immobilization.
Speakers
JL

Jean-Maude Louizias

University of Florida
Co-authors
CC

Carlene Chase

University of Florida
GM

Gabriel Maltais-Landry

University of Florida
NA
JD

Johan Desaeger

University of Florda
NA
NB

Nathan Boyd

University of Florida
NA
RK

Rosalie Koenig

University of Florida
NA
Wednesday July 30, 2025 5:00pm - 5:15pm CDT
Foster 2

5:15pm CDT

WCPM 2 - Bed Architecture and Fumigation Strategies for Strawberries in Eastern NC
Wednesday July 30, 2025 5:15pm - 5:30pm CDT
Management of plant parasitic nematodes (PPNs), soil-borne diseases, and weeds in the strawberry industry is fundamental for every North Carolina grower, to achieve a successful production season. The prohibition of methyl bromide (MBr) in 2005 caused significant challenges for growers leading to the search for new and efficient strategies to suppress and control the impact of pests and diseases that affect strawberry growers. Hence, the objective of this project was to evaluate alternative fumigation strategies combined with new bed architectures for the control of soil-borne pests in strawberry production. Two experiments were conducted in North Carolina where, compact beds (24 inches wide by 12 inches tall) were compared to traditional North Carolina strawberry beds (30 inches wide by 6 inches tall). Both bed architecture designs were fumigated with either shank or drip-applied fumigants (Pic-Clor 60 and Pic-Clor 60 EC, respectively), with and without the inclusion of a soil surfactant for the drip fumigation treatments. In both studies, we measured strawberry plant biomass, volumetric water content in multiple points of the planting beds, plant parasitic nematodes (PPNs) and weed pressure before and after fumigation, plant reflectance indexes, bed compaction, and crop yield. Post-treatment results demonstrated that compact beds with both shank and drip-applied fumigants achieved comparable control of weeds and PPNs to traditional beds with shank fumigation. Bed compaction measures indicated that compact bed treatments present less compaction at 4-, 8- and 12-inch depths compared to the higher compaction observed in North Carolina’s traditional strawberry beds.
Speakers
JA

Josue Alarcon Mendoza

North Carolina State University, Horticultural Science
Co-authors
ET

Emmanuel Torres Quezada

North Carolina State University
Wednesday July 30, 2025 5:15pm - 5:30pm CDT
Foster 2

5:30pm CDT

WCPM 2 - Residual herbicide degradation on nursery trees following drift events
Wednesday July 30, 2025 5:30pm - 5:45pm CDT
Minnesota (MN) in the US ranks 2nd for corn silage production and 3rd for soybean production. Studies have shown that off-target movement (drift) from an unshielded sprayer, in row crops, can range from 1 to 16% of the target dose; however, boom height and wind can double or triple these doses. Pre-study 37 pesticide residue tests were conducted in five Minnesota nurseries in 2018. Nine different herbicides were detected in the 37 tests. Glyphosate (sold as Roundup Power Max 32% glyphosate) became the focus of 2019 studies, conducted at concentrations of 0, 6, 18 and 54% for glyphosate as drift events. Relating ppm’s disclosed in laboratory analyte foliar residue samples collected by various state agriculture departments after drift events to injury symptom, development, and growth reduction or time after application have ever been conducted for nursery crops. From personal communication with nursery owners experiencing neighbor’s drift event (MD; KY, OH, MI, FL) the average time from the drift event to recognizing problems, and sample collection is six weeks (6 weeks after drift event) (6 WADE). Slower metabolism of glyphosate at higher residues, explained our finding that the largest residues were detected in all, but one species/ treatments used with glyphosate, versus three other herbicides examined or in combinations. Higher residues meaning higher injury, does not occur with glyphosate. Potentially high residues mean slow metabolism for glyphosate, and thus less injury. In five cases glyphosate applied alone had no impact on growth measured as caliper over the season, even though glyphosate had the highest residue in three of the five. In only one case was caliper growth reduction highest with glyphosate (Syringa 18%) over the entire study. In terms of phytotoxicity rating, glyphosate caused no commercially unacceptable injury in four cases. Residues increased with all rates on the three species evaluated between 3 DADE and 6 WADE. With Acer freemanii and Tilia cordata residue levels declined slightly between 6 WADE to 14 WADE with all rates. Only, Syringa reticulata showed an increase in foliar residues between 6 WADE to 14 WADE. Since carry over injury the next year (or three) is a concern with all glyphosate applications to perennial plants, all trees were observed in 2020. None were commercially acceptable and in severe decline (regardless of rate) in spring 2020, only 6% glyphosate on Syringa survived to the fall , and by spring 2021 were dead.
Speakers
HM

Hannah Mathers

Mathers Environmental Science Services, LLC
Owner of Mathers Environmental Science Services, established 2010, active since 2015 resigning from Ohio State University, as Full Professor/ State Extension Nursery/ Landscape Extension Specialist 2000 to 2015. Previous to OH State University was Assistant Professor and Regional... Read More →
Co-authors
JB

James Beaver

Mathers Environmental Science Services, LLC
NA
Wednesday July 30, 2025 5:30pm - 5:45pm CDT
Foster 2
  Oral presentation, Weed Control and Pest Management 2
 
Thursday, July 31
 

7:59am CDT

PNM 3 - Oral Session
Thursday July 31, 2025 7:59am - 8:00am CDT
Presiding/Moderator
EM

Elena Maximo

University of Florida
Thursday July 31, 2025 7:59am - 8:00am CDT
Foster 2

8:00am CDT

PNM 3 - Evaluating impact of organic amendments on broccoli transplant production
Thursday July 31, 2025 8:00am - 8:15am CDT
Transplants provide an early start and help with better establishment and enhanced yield and quality in vegetable crops. For production of healthy and high-quality transplants, nutrient management is critical. There are several organic amendments available that can be directly added to the transplant growing media to provide essential nutrients for transplant growth and development. Some of the commonly used amendments include blood meal, bone meal, feather meal, fish emulsion, alfalfa meal, crab meal, shrimp meal, bat guano, etc., These organic amendments are obtained from dried animal blood or bone products which are rich in nutrients like Nitrogen, Phosphorus, and Potassium. Even though these amendments are available, there is lack of information on their efficacy and impact in organic transplant production. This greenhouse study conducted at Iowa State University, investigated the use of several amendments in broccoli transplant production. Treatments included: blood meal, bone meal, feather meal, fish emulsion, compost, conventional fertilizer, compost, and a no-fertilizer control. Plant height, stem diameter, SPAD (Soil Plant Analysis Development), dry biomass, and root length are the parameters we analyzed to determine the transplant growth. The findings show that the conventional fertilizer treatment had the tallest plant, with a mean height of 8.54 cm, followed by the blood meal with a height of 8.15 cm. No significant difference was observed between the conventional fertilizer and blood meal treatment. The highest mean stem diameter was found in the blood meal treatment, measuring 4.2 mm, followed by the feather meal treatment, which had a mean stem diameter of 3.65 mm. Blood meal and feather meal treatments were significantly different from each other. The plant biomass was high in the blood meal treatment at 3.36g. The blood and feather meal treatments differed significantly from the bone meal, compost, fish emulsion, and control treatments. Results from this study show that organic amendments can be successfully used to improve the transplant growth with a single application, potentially saving labor and cost. Keywords: transplants, organic amendments, SPAD, nutrients, blood meal, bone meal, feather meal.
Speakers
AT

Akshaya Thinakaran

Iowa State University
Co-authors
AN

Ajay Nair

Iowa State University
NA
Thursday July 31, 2025 8:00am - 8:15am CDT
Foster 2

8:15am CDT

PNM 3 - Effect of Planting Dates and Nitrogen Rates on Broccoli in North Carolina
Thursday July 31, 2025 8:15am - 8:30am CDT
Shifts in regional precipitation patterns and rising temperatures due to climate change have increased uncertainty surrounding optimal planting windows and nitrogen (N) management strategies for broccoli production in North Carolina (NC). In particular, growers face challenges in aligning planting schedules with ideal environmental conditions and adjusting N application rates to match crop demands under variable temperature regimes. Given that both air and soil temperatures directly influence crop nutrient uptake and development, this study aimed to evaluate the combined effects of planting date, nitrogen rate, and application timing on the growth and productivity of two commercially important broccoli cultivars: 'Eastern Crown' and 'Emerald Crown.' Field experiments were conducted at the Horticultural Crops Research Station of North Carolina State University in Clinton, NC. The first trial included four planting dates—early March, late March, early April, and late April—combined with three fertigation N rates of 120, 100, and 80 lb/acre. The second trial focused on two planting dates, late March and late April, combined with four pre-plant N application rates: 0%, 20%, 40%, and 60% of total recommended N. Across both trials, plant performance was assessed through measurements of canopy volume, aboveground biomass, leaf greenness via Normalized Difference Vegetation Index (NDVI), tissue nutrient content, and marketable yield. Results show that early planting dates were associated with lower accumulation of Growing Degree Days (GDD), which may have limited early crop development. In contrast, later planting dates generally experienced higher GDD accumulation, improving crop rate of growth. Higher N application rates were positively correlated with increased NDVI values, biomass, and yield. Reduced NDVI readings under lower N rates indicate a possible decline in plant vigor and photosynthetic activity. Additionally, strategic pre-plant N applications showed potential benefits in improving early plant establishment and nutrient status, particularly under later planting dates. Overall, our findings show the importance of optimizing both planting timing and N management strategies to improve broccoli productivity in NC. Tailoring N rates based on planting date and anticipated thermal conditions may contribute to more resilient production systems under changing climate conditions.
Speakers
TQ

Tomas Quezada Baez

North Carolina State University
Thursday July 31, 2025 8:15am - 8:30am CDT
Foster 2

8:30am CDT

PNM 3 - Evaluating the effect of SAFEROCK micronized for nutrient use efficiency, growth, and yield in tomato and sweet corn
Thursday July 31, 2025 8:30am - 8:45am CDT
SafeRock micronized is a 100% natural mineral soil enhancer and fertilizer booster mined from unique sandstone deposits in United Kingdom and contains a balanced blend of over 70 nutrients and clay minerals. A field experiment was conducted at Blackville SC to evaluate the ability of SafeRock micronized to improve nutrient use efficiency, growth and yield of tomato and sweet corn. The experiment comprised of seven treatments [normal farming practices (NFP), NFP SafeRock soil mix (50 and 75 kg/ha.), NFP SafeRock fertigation (50 and 75 kg/ha.), 80% N
Speakers
BJ

Bhupinder Jatana

Assistant Professor -Horticulture, Clemson University
Co-authors
TS

Tom Sanders III

Clemson University
NA
Thursday July 31, 2025 8:30am - 8:45am CDT
Foster 2
  Oral presentation, Plant Nutrient Management 3

8:45am CDT

PNM 3 - Evaluation of Critical Nitrogen Requirements for Commercial Tomato Production in MS
Thursday July 31, 2025 8:45am - 9:00am CDT
Ensuring nitrogen applications targeting the optimum rate is critical in tomato production. This is because nitrogen (N) requirements in tomato production may vary depending on the soil type, location, climate, and cultivation practices. Despite tomato being a major vegetable crop in MS, information on the N requirements for commercial production in the state is limited. Therefore, this study was conducted in an open field to determine the critical nitrogen rate for commercial tomato production under the northeast growing conditions of MS. The study evaluated six N application rates (0, 67, 101, 135, 202, and 269 kg ha-1) arranged in a randomized complete block design with four replicates per treatment. Red Duce variety of tomato was planted, and nitrogen was applied at 50% pre-plant (33-0-0) and 50% fertigation (15.5-0-0). Both potassium and phosphorus were the same for all treatments and applied at 100% pre-plant using 0-0-60 and 0-46-0 respectively. Preliminary results indicate that higher N application rates significantly increased total fruit yield, total marketable yield, fruit sizes, and total biomass. The highest total fruit yield (93.79 t ha-1), total marketable yield (71.58 t ha-1), large fruit yield (10.40 t ha-1), and total biomass (6.96 t ha-1) were observed at 269 kg N ha-1. Increasing N application rates significantly increased tissue N concentration. Therefore, the greater yield observed at higher N application rates was attributed to the consistently higher tissue N concentration from leaf samples collected at different weeks during the growing season. Based on the results obtained, the critical N application rate for open-field fresh-market tomato production in northeast MS could be up to 269 kg ha-1. However, follow-up studies are currently being conducted to validate these preliminary results.
Speakers
PK

Prakash Khanal

Mississippi State University
Co-authors
IA

Ibukun Ayankojo

Mississippi State University
Thursday July 31, 2025 8:45am - 9:00am CDT
Foster 2

9:00am CDT

PNM 3 - Determining the Phosphorus Fertilizer Application Threshold for Potato (Solanum tuberosum L.) Based on Nutritional Requirement in Subtropical Spodosol of Northeast Florida
Thursday July 31, 2025 9:00am - 9:15am CDT
Phosphorus (P) is a macronutrient essential for plant growth and yield; however, its availability in spodosols is often limited due to high acidity, low organic matter, and strong adsorption to soil minerals, particularly iron (Fe) and aluminum (Al) in tropic Spodosol in Northeast Florida. Efficient P management is essential for optimizing crop productivity while minimizing environmental risks. This study investigated the effects of different P fertilizer rates on Potato (Solanum tuberosum, L.) growth, yield, and nutrient dynamics at the UF/IFAS Hastings Agricultural Extension Center, Northeast Florida over two consecutive growing seasons (Spring 2022 and Sprin 2023). A randomized complete block design was employed, with five P rates of 0, 90, 135, 180, and 225 kg ha-1 of phosphorus pentoxide (P2O5) as triple superphosphate (TSP). The changes in plant growth, nutrient uptake, yield, as well as soil properties were determined to evaluate the effectiveness of P fertilization as plant growth performance and tuber yield. The results showed that P application significantly increased potato tuber yield compared to the control, with the highest yield observed at 225 kg ha-1 P2O5. Pearson correlation analysis indicated strong associations between plant growth, tuber yield, and nutrient accumulation. Principal component analysis (PCA) highlighted notable seasonal differences in tuber yield and soil characteristics. The study highlights the importance of site-specific P recommendation to synchronize nutrient availability with crop demand, particularly in nutrient-poor spodosols in Northeast Florida. These findings provide a scientific basis for establishing P fertilizer thresholds that balance crop productivity with environmental sustainability in agricultural systems.
Speakers
avatar for Md Jahidul Islam Shohag

Md Jahidul Islam Shohag

University of Florida
Co-authors
GL

Guodong Liu

University of Florida
Thursday July 31, 2025 9:00am - 9:15am CDT
Foster 2
  Oral presentation, Plant Nutrient Management 3
  • Subject Plant Nutrient Management
  • Poster # csv
  • Funding Source Florida Department of Agriculture and Consumer Services
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

9:15am CDT

PNM 3 - Effectiveness of Hydrogel Polymer Combined with Phosphorus on Snap Bean (Phaseolus vulgaris) Growth and Yield.
Thursday July 31, 2025 9:15am - 9:30am CDT
Phosphorus is well known for being a nutrient vital for plants, influencing key stages of plant cycle. However, its efficiency can be reduced by nutrient fixation processes or leaching, with challenging strategies to enhance phosphorus availability. Hydrogel polymers have been proposed as soil amendments to improve moisture retention and nutrient uptake. This study evaluates the impact of hydrogel polymer combined with different phosphorus rates on the growth and yield of Snap Bean (Phaseolus vulgaris). The experiment consists of six phosphorus rates of 0, 100, 150, 200, 250, and 300 lbs/A P2O5, and a hydrogel rate of 30 lbs/A. Treatments were arranged in a randomized complete block design with 4 replications per treatment, and 4 rows per replication. Growth parameters such as Plant Height, Leaf chlorophyll content, weekly tissue sampling. Yield parameters such as number of pods per plant, pod weight, pod yield were also assessed. Data analysis is performed using one-way ANOVA in R Studio, with post-hoc comparisons using Tukey’s HSD test at P
Speakers
EM

Elena Maximo

University of Florida
Co-authors
GL

Guodong Liu

University of Florida
MJ

Md Jahidul Islam Shohag

University of Florida
Thursday July 31, 2025 9:15am - 9:30am CDT
Foster 2
  Oral presentation, Plant Nutrient Management 3

9:30am CDT

PNM 3 - Enhancing Snap Bean (Phaseolus vulgaris L.) Growth and Yield through Liquid Oxygen and Phosphorus Fertigation Under Fiel
Thursday July 31, 2025 9:30am - 9:45am CDT
Snap bean (Phaseolus vulgaris L.) is a vital crop in Florida, but it is highly vulnerable to low-oxygen (O₂) stress in soil and phosphorus (P) deficiency, particularly in regions utilizing deep well irrigation. The objective of this study was to evaluate the effects of liquid O₂ and P fertigation on plant growth, pod yield, and nutrient uptake under field conditions. A Randomized Complete Block Design (RCBD) with four replications was employed, testing two rates of liquid O₂ (0 and 45 kg ha⁻¹ as hydrogen peroxide, H₂O₂) and five levels (0, 90, 135, 180, and 225 kg ha⁻¹) of liquid P as 0-54-0 phosphoric acid (H₃PO₄). Key variables assessed included plant growth, P uptake, pod yield, and soil properties to evaluate the effectiveness of fertigation in promoting plant performance and nutrient uptake. The results indicated that both liquid O₂ and P applications enhanced plant growth and pod yield, with the highest pod yield achieved from the combination of 45 kg ha⁻¹ of liquid O₂ and 225 kg ha⁻¹ of liquid P. Pearson correlation analysis revealed strong associations between plant growth, pod yield, and nutrient uptake. Principal component analysis (PCA) confirmed the significant effects of liquid O₂ and P fertigation on growth parameters and pod yield. This study concludes that liquid O₂ fertigation alleviates soil hypoxia, improves P use efficiency, and boosts crop productivity, offering a promising and cost-effective solution for optimizing snap bean cultivation.
Speakers
avatar for Md Jahidul Islam Shohag

Md Jahidul Islam Shohag

University of Florida
Co-authors
EM

Elena Maximo

University of Florida
GL

Guodong Liu

University of Florida
Thursday July 31, 2025 9:30am - 9:45am CDT
Foster 2
  Oral presentation, Plant Nutrient Management 3
  • Subject Plant Nutrient Management
  • Poster # csv
  • Funding Source Florida Department of Agriculture and Consumer Services
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

9:45am CDT

PNM 3 - Nutrient Solubility in Response to Root Zone pH for Soilless Plant Culture
Thursday July 31, 2025 9:45am - 10:00am CDT
The pH of the root zone plays a crucial role in influencing the solubility of nutrients within a soilless substrate or hydroponic solution, affecting their uptake into plant tissue. Our objective was to re-evaluate and update the understanding of how root zone pH affects nutrient solubility in soilless substrates and hydroponic systems using chemical equilibria software, laboratory testing, and a literature review. The aim was to provide horticulture practitioners with an understanding of the trends that should be considered when managing substrates and fertilization. Calcium and magnesium have decreasing solubility with increasing pH. However, dolomitic limestone added for neutralization of acidic substrates such as bark and peat increase Ca and Mg under alkaline conditions. Because potassium salts are usually the liming sources in hydroponics, Ca and Mg levels decrease at high pH. Phosphorus availability is limited primarily because of reactions with Ca at high pH. Results demonstrated substantial differences in micronutrient solubility between sulfate-based and chelated micronutrients. In hydroponic simulations (GEOCHEM-EZ), sulfate-based micronutrients rapidly became insoluble at higher pH (>6.0), whereas chelates, particularly Fe-EDDHA (ethylenediamine-N,N-bis(2-hydroxyphenylacetic acid), maintained solubility even under alkaline conditions (up to pH 10.0). Visual MINTEQ simulations highlighted the role of DOM in stabilizing micronutrient availability in soilless substrates through the formation of metal-organic complexes, especially for metal micronutrients Fe, Cu, and Zn. Laboratory measurements were consistent with chemical equilibrium simulations, demonstrating higher water-extractable micronutrient solubility with EDTA (ethylenediaminetetraacetic acid) chelates compared with sulfate forms, particularly at elevated pH. Fe and Mn solubility sharply declined with increasing substrate pH, whereas Zn and Cu solubility peaked at intermediate pH levels (5.5 to 6.5). Boron availability decreased as substrate pH increased, whereas Mo solubility increased under alkaline conditions. A review of trends in plant tissue micronutrient concentration at different pH levels in hydroponics and soilless substrates emphasized that translating micronutrient solubility to plant availability is complex. For example, absorption of cations by roots at very low pH may be impeded in soilless substrates hydroponics by a combination of growth inhibition, nutrient disorders, and direct pH effects of H concentration. Plant uptake involves numerous biological processes, including root exudation, microbial interactions, nutrient competition, redox conditions, and plant-specific nutrient strategies. Updated pH solubility charts were developed to help visualize micronutrient solubility and plant uptake under different scenarios that have practical implications for horticulture fertilizer management.
Speakers
PF

Paul Fisher

University of Florida
Co-authors
JH

Jinsheng Huang

University of Florida
NA
Thursday July 31, 2025 9:45am - 10:00am CDT
Foster 2
  Oral presentation, Plant Nutrient Management 3
  • Subject Plant Nutrient Management
  • Poster # csv
  • Funding Source Floriculture Research Alliance, USDA-ARS Floriculture and Nursery Research Initiative #58-3607-8-725, USDA NIFA multi-state NC1186, and HATCH FLA-ENH-005918

10:14am CDT

PGR - Oral Session
Thursday July 31, 2025 10:14am - 10:15am CDT
Presiding/Moderator
HG

Haramrit Gill

University of California Agriculture and Natural Resources
NA
Thursday July 31, 2025 10:14am - 10:15am CDT
Foster 2

10:15am CDT

PGR - Overcoming the Pre-Harvest Fruit Drop vs. Fruit Color Trade-off in 'Honeycrisp' Apples Using Combined Plant Growth Regul
Thursday July 31, 2025 10:15am - 10:30am CDT
Pre-harvest fruit drop (PFD) causes significant yield losses in commercially valuable apple cultivars like 'Honeycrisp'. Ethylene, the master regulator of various fruit ripening traits in climacteric fruits, accelerates pre-harvest fruit drop. While ethylene inhibitors such as aminoethoxyvinylglycine (AVG) effectively reduces PFD, it can negatively impact fruit color development, a process also regulated by the plant hormone ethylene. To address this trade-off, we evaluated various plant growth regulators (PGRs), such as Accede (1-aminocyclopropane-1-carboxylic acid (ACC)-10%), Motivate (ethephon-21.7%), ReTain (AVG-15%) and their combinations, in ‘Honeycrisp’ apples over two consecutive years (2023
Speakers Co-authors
KJ

khalil Jahed

Virginia Tech
SS

Sherif Sherif

Virginia Tech
Dr. Sherif M. Sherif is an Associate Professor at Virginia Tech, where he channels his fervent zeal for life sciences and educational outreach into his specialization in tree fruit physiology and molecular biology. His career is defined by a dedication to overcoming the biological... Read More →
Thursday July 31, 2025 10:15am - 10:30am CDT
Foster 2

10:30am CDT

PGR - Testing of 1-Aminocyclopropane-1-Carboxylic Acid (ACC) for Chemical Removal of Flowers of Young Orchard Trees Aiming at
Thursday July 31, 2025 10:30am - 10:45am CDT
Approximately 3-5% of perennial orchard trees are replanted annually. Bearing fruit on newly planted orchard trees is undesirable because fruits are strong carbohydrate sinks and compete with shoot growth, and thus limit early growth of orchard trees to fill their space. Therefore, manual removal of flowers and fruitlets from newly planted trees has been practiced. In almond, leaving nuts on young trees cannot just limit vegetative growth but also serve as harboring site for navel orange worm. Therefore, removal of flowers and fruitlets/nutlets via spray application of chemical thinners would be highly desirable to aim tree growth and reduce manual labor cost for tree defruiting. In a series of experiments in nectarines, almonds and walnuts in California, we investigated the effects of the application of ACC alone or in combination with S-abscisic acid (S-ABA), 6-benzyladenine (6-BA) on flower and fruitlet/nutlet removal. Results indicated that ACC by itself at 450-600 mg/L or in combination with 300 mg/L S-ABA or 400 mg/L 6-BA can effectively defruit young orchard trees and reduce manual labor cost. No phytotoxicity was observed in any of the trials.
Speakers
JR

Jozsef Racsko

Valent USA
Co-authors
PF

Poliana Francescatto

Valent BioSciences
NA
Thursday July 31, 2025 10:30am - 10:45am CDT
Foster 2

10:45am CDT

PGR - The effect of preharvest ethylene regulator treatments on ‘Ambrosia’ fruit drop and crack, maturity, quality and related
Thursday July 31, 2025 10:45am - 11:00am CDT
‘Ambrosia’ is an emerging apple cultivar recently introduced from British Columbia and knowledge of ripening and preharvest physiology is limited in the mid-Atlantic region. ‘Ambrosia’ must meet minimum quality and color marketability standards in the US though it requires a high temperature differential for full color development and has a short harvest period. Plant growth regulators, such as aminoethoxyvinylglicine (AVG) and 1-methylcyclopropene (1-MCP), can alter preharvest fruit drop, maturity and quality by hindering ethylene biosynthesis and perception, respectively. AVG has historically been used at several rates by mid-Atlantic growers with variable cultivar specific impacts. The objective of this study was to characterize and compare the effects of AVG and 1-MCP on preharvest fruit drop and crack, ethylene production, fruit physicochemical parameters, skin color, and transcript accumulation of ethylene and anthocyanin-related genes in 'Ambrosia' apples throughout on-the-tree ripening over two production seasons. Four treatments were established and applied based on manufacturer’s recommendations: full-rate AVG (Retain), half-rate AVG (Retain), 1-MCP (Harvista) and a control. Fruits were harvested at three maturity stages: 1 week before the anticipated commercial harvest (1WBCH), at the anticipated commercial harvest (CH), and one week after CH (CH 1). Our results showed that both AVG and 1-MCP treatments significantly reduced preharvest fruit drop and crack with respect to control fruit. While full-rate AVG treatment showed the most significant decrease in ethylene production and exhibited the greatest downregulation of ethylene biosynthesis and perception-related gene expression, it also negatively impacted red color and anthocyanin biosynthesis related gene expression and reached minimum 50% skin blush one week later than the other treatments. Half-rate AVG and 1-MCP treatments displayed an intermediate behavior between full-rate AVG and control fruit, delaying fruit maturity and the associated gene expression when compared to control, but without sacrificing skin blush development. This study contributes to understand how preharvest ethylene regulator treatments influence ‘Ambrosia’ apples grown under the mid-Atlantic environmental conditions.
Speakers
MF

Macarena Farcuh

University of Maryland
Co-authors
EJ

Emily Johnson

University of Maryland
NA
Thursday July 31, 2025 10:45am - 11:00am CDT
Foster 2
  Oral presentation, Plant Growth Regulation
  • Subject Plant Growth Regulation
  • Poster # csv
  • Funding Source State Horticultural Association of Pennsylvania and startup funds awarded to M.Farcuh, University of Maryland

11:00am CDT

PGR - Ethylene-Generating Compounds for Chemical Thinning: Comparative Evaluation of 1-Aminocyclopropane-1-Carboxylic Acid (AC
Thursday July 31, 2025 11:00am - 11:15am CDT
Ethylene plays a significant role in flower and fruitlet abscission. The use of compounds that generate ethylene for chemical thinning of fruit trees has been in the focus of research for decades. 1-aminocyclopropane-1-carboxylic acid (ACC) is a naturally occurring precursor to ethylene and has recently been registered as a chemical thinner in the US and elsewhere for stone fruit and apple. Ethephon has also been evaluated for chemical thinning of peaches but phytotoxicity and inconsistency in efficacy has hindered its commercial use. With the recent registration of ACC for chemical thinning of peaches, questions arose if similar efficacy can be achieved with the use of ethephon. The objective of the study reported here was to compare thinning efficacy and phytotoxicity of ACC (300 mg/L) and ethephon (428 mg/L; molar equivalent to 300 mg/L ACC) when used in peaches. A field experiment was conducted in Red Top peach variety in Oregon where the two compounds were sprayed to different set of trees every day from pink bud to petal fall to evaluate the effect of daily weather conditions on flower abscission. Phytotoxicty was also recorded in the experiment. ACC and ethephon showed similar thinning pattern throughout the flowering time. While no phytotoxicity was observed for ACC, severe leaf drop and gummosis occurred in the ethephon treatments. The presentation will discuss the potential reasons for the efficacy and difference in level of phytotoxicity between the two compounds.
Speakers
JR

Jozsef Racsko

Valent USA
Co-authors
PF

Poliana Francescatto

Valent BioSciences
NA
Thursday July 31, 2025 11:00am - 11:15am CDT
Foster 2

11:15am CDT

PGR - Quantifying the Effects of Prohexadione-calcium Foliar Spray and Substrate Drench Applications on Perennial Ornamental G
Thursday July 31, 2025 11:15am - 11:30am CDT
Our objective was to quantify prohexadione-calcium foliar spray or substrate drench applications to control growth of two perennial ornamental grass taxa. On 6 June 2024, 38-cell liner trays (126-mL individual cell volume) of Andropogon gerardii ‘Red October’ and Miscanthus sinensis ‘Fire Dragon’ were received from a commercial propagator. Liners were transplanted with one plant per 19.7-cm-diameter container (1.7 L volume) filled with a commercially available bark-based substrate comprised of (by vol.) 56.5% aged pine bark, 34.8% Canadian sphagnum peatmoss, and 8.7% perlite, dolomitic limestone, a nutrient starter charge, and a wetting agent. Plants were grown at bench-level in a glass-glazed greenhouse under supplemental and day-extension lighting provided by 1000-W light-emitting diode lamps from 0600 to 2200 HR (16-h photoperiod) with an air temperature set point of 20 °C. At 7 d after transplant, 10 single-plant replicates of each taxa were treated with a solution containing prohexadione-calcium and a water conditioning agent. Plants received either a foliar spray (vol. 0.2 L·m–2) solution containing deionized water (0 mg·L–1; untreated) or 250, 500, 750, 1,000, or 2,000 mg·L–1 prohexadione-calcium or a substrate drench of 296-mL aliquots of solution containing 0, 125, 250, 500, or 1,000 mg·L–1 prohexadione-calcium. At eight weeks after treatment, plant height, plant diameter, and shoot dry weight (SDW) were determined for all plants, and root dry weights (RDW) were determined for a sub-sample (n = 5) of each taxa from each substrate drench treatment. Foliar spray applications of 125 to 2,000 mg·L–1 prohexadione-calcium did not affect growth of either perennial ornamental grass taxa. Substrate drenches were effective in controlling growth of each perennial ornamental grass taxa although the magnitude of response varied among taxa. For example, compared to untreated plants, plant height, plant diameter, SDW, and RDW of ‘Red October’ Andropogon drenched with 125 to 1,000 mg·L–1 prohexadione-calcium decreased by 20% to 57% (19.7 to 55.9 cm), 0% to 43% (7.8 to 33.4 cm), 3.6 to 28.9 g (10% to 81%), and 10.4 to 28.5 g (27% to 74%), respectively. This research demonstrated substrate drench applications of prohexadione-calcium to be effective for controlling growth, while further research is warranted to determine optimal concentrations for foliar spray applications. We suggest substrate drench applications of 125 to 500 mg·L–1 prohexadione-calcium as an initial range for trials to control growth of ornamental perennial grasses.
Speakers
avatar for Garrett Owen

Garrett Owen

Extension Specialist and Assistant Professor of Sustainable Greenhouse & Nursery Systems, The Ohio State University
Dr. W. Garrett Owen is Assistant Professor and Extension Specialist of Sustainable Greenhouse and Nursery Production Systems with primary responsibilities in teaching (20%), research (30%), and Extension (50%) in the Department of Horticulture and Crop Science at The Ohio State University... Read More →
Co-authors
LW

Lark Wuetcher

The Ohio State University
NA
Thursday July 31, 2025 11:15am - 11:30am CDT
Foster 2
  Oral presentation, Plant Growth Regulation
  • Subject Plant Growth Regulation
  • Poster # csv
  • Funding Source We thank Fine Americas, Inc. for the plant growth regulator and financial support; Sun Gro Horticulture for soilless substrate; and J.R. Peters, Inc. for fertilizer.

11:30am CDT

PGR - Evaluating the Impact of Foliar Indole-3-butyric Acid on the Adventitious Rooting of Ornamental Woody Nursery Taxa
Thursday July 31, 2025 11:30am - 11:45am CDT
The propagation of ornamental woody nursery liners (young plants) is primarily through stem cuttings to maintain genotypic and phenotypic uniformity. The application of rooting hormones is common practice in stem cutting propagation to hasten, promote uniformity, and enhance the quality of adventitious rooting. Rooting hormones are traditionally applied by dipping cuttings into solutions or powders. However, these methods increase labor costs, cutting stress, and opportunities for contamination. Foliar applications of rooting hormones would improve production efficiency, reduce cutting contamination and stress, and allow for multiple hormone applications after sticking. Thus, there is a critical need to identify the optimal rates of foliar rooting hormones to improve and maximize adventitious rooting in woody nursery taxa. Therefore, this study aimed to quantify the impact of foliar rooting hormones on root growth and development of ornamental woody nursery cuttings. Unrooted stem cuttings of four ornamental nursery taxa were obtained from commercial nurseries and individually inserted into 6.4-cm diameter containers filled with a propagation substrate. Cuttings were placed in a diffused glass-glazed greenhouse under a propagation environment of fixed 4-mil clear construction film. The greenhouse was set at 20 °C air temperature, 80% relative humidity, and ambient daylight supplemented with ≈120 µmol·m–2·s–1 delivered from light-emitting diode arrays from 0600 to 2200 hr. At 1 d after sticking, cuttings were sprayed with a solution containing clear tap water and 0, 500, 1,000, 1,500, 3,000, or 6,000 mg·L–1 20% indole-3-butyric acid (IBA) at a volume of 1.89 L·m–2. After 42 d, data was collected including callusing and rooting percentage, stem length and caliper, leaf area, and shoot and root dry mass. In general, increasing IBA foliar application concentration improved rooting success and uniformity to different magnitudes among species. For example, rooting percentage of Chamaecyparis increased by 67% as foliar IBA concentrations increased from 0 to 6,000 mg·L–1 IBA. The results of this study determined the optimal rates of foliar IBA application across an array of woody taxa to hasten adventitious rooting and improve quality for ornamental woody nursery liner production.
Speakers
OL

Olivia Liebing

The Ohio State University
Co-authors
GO

Garrett Owen

The Ohio State University
Thursday July 31, 2025 11:30am - 11:45am CDT
Foster 2
  Oral presentation, Plant Growth Regulation
  • Subject Plant Growth Regulation
  • Poster # csv
  • Funding Source The Ohio State University College of Food, Agricultural, and Environmental Sciences - New Investigators Grant; USDA Agricultural Research Service - Floriculture & Nursery Research Initiative 5082-21000-001105S

11:45am CDT

PGR - Night Interruption UV-A Radiation Influences Plant Architecture and Biomass Accumulation of Potted Spearmint and Greek O
Thursday July 31, 2025 11:45am - 12:00pm CDT
A high-quality containerized culinary herb is one that is proportional to its container with a compact growth habit and stems or leaves that are not excessively elongated. However, chemical plant growth regulators (PGRs) used to modify plant growth, increase branching, or reduce stem elongation are not labeled for use on culinary herbs. A novel option for reducing stem elongation could be exposure to night-interruption (NI) ultraviolet (UV) radiation. Therefore, our objectives were to 1) quantify the influence of UV-A radiation on the growth, stem elongation, and relative chlorophyll concentration of containerized spearmint (Mentha spicata) and Greek oregano (Origanum vulgare var. hirtum) and 2) determine the most effective duration and sequence of exposure. Rooted cuttings were transplanted into 15-cm containers and placed in a greenhouse at 23 °C and under a 16-h long-day (LD) provided by LED supplemental lighting (SL) or under a 9-h short-day (SD) and 6-h of NI lighting providing 385 nm of UV-A radiation at an intensity of 20 µmol∙m–2∙s–1 for 4 weeks. The 10 treatments included: LD entire time, LD 2 week-UV 2 weeks, LD 1.5 weeks-UV 2.5 weeks, LD 2 weeks-UV 2 weeks, LD 2 week-UV 1 weeks-LD 1 week, UV entire time, UV 2 weeks-LD 2 weeks, UV 2.5 weeks-LD 1.5 weeks, UV 2 weeks-LD 2 weeks, or UV 2 week-LD 1 week-UV 1 week. Exposure to SD UV-A NI for 4 weeks, resulted in a ≈ 25 and 29% reduction in height of Greek oregano and spearmint, respectively. Furthermore, when LDs were the last treatment spearmint plants received, they had a higher chlorophyll content than those exposed to UV-A. Our results indicate that UV-A NI lighting for spearmint and Greek oregano may be an option for height control in controlled environments.
Speakers
RE

Rose Elbert

Michigan State University
Co-authors
RL

Roberto Lopez

Michigan State University
NA
Thursday July 31, 2025 11:45am - 12:00pm CDT
Foster 2
  Oral presentation, Plant Growth Regulation
  • Subject Plant Growth Regulation
  • Poster # csv
  • Funding Source USDA SCRI
  • Funding Option SCRI funded all or part of the research associated with this abstract

12:00pm CDT

PGR - Ethephon Drench Efficacy is Not Affected in Sphagnum Peat-Based Substrates Amended with Perlite, Aged Pine Bark, or Wood
Thursday July 31, 2025 12:00pm - 12:15pm CDT
Greenhouse substrates are soilless mixes formulated by blending organic and inorganic components at varying proportions. Substrate properties are known to influence the efficacy of plant growth regulators applied as a substrate drench. Ethephon is a plant growth regulator used in floriculture production to control stem elongation, increase lateral branching, and manipulate flowering. Ethephon substrate drenches have been shown to control growth in annual bedding plants and herbaceous perennials; however, there is a lack of research examining how substrate composition influences the efficacy of ethephon substrate drenches. Therefore, the objective of this research was to evaluate ethephon drench efficacy in peat-based substrates amended with perlite, aged pine bark, or wood fiber. Lantana (Lantana camara L. ‘BandoleroTM Pink’ and ‘New Gold’) were transplanted into 15.2-cm containers filled with one of six peat-based substrates amended (by vol.) with 20% or 40% perlite, aged pine bark, or wood fiber. After 10 d, eight single-plant replicates received a drench of 150 mL aliquots of solution containing 0, 25, 50, 75, 100, or 200 mg·L–1 ethephon. Plants were grown in a glass-glazed greenhouse for 5 weeks after drench before growth data, including plant height, plant diameter, shoot dry weight (SDW), and root dry weight (RDW) were determined. Increasing concentrations of ethephon decreased plant height, plant diameter, SDW, and RDW of both cultivars of lantana. Substrate components and formulation had varied effects on plant growth. For example, in Expt. 1, plant height was unaffected by formulation but the component × concentration interaction was significant. When substrates were formulated with perlite, aged pine bark, or wood fiber plants were 23% (3.9 cm), 13% (2.0 cm), and 18% (3.1 cm) shorter, respectively, as concentrations increased from 0 to 200 mg·L–1 ethephon, compared to untreated plants. In Expt. 2, neither component or formulation significantly influenced plant height, and when drenched with 200 mg·L–1 ethephon plant diameter was 42% (15.8 cm) less than untreated plants. In Expt. 1, SDW was not influenced by formulation but the component × concentration interaction was significant. For instance, compared to untreated plants, SDW of plants grown in substrates containing perlite, aged pine bark, or wood fiber were 42% (3.8 g), 23% (1.7 g), and 35% (2.7 g) smaller, respectively, as concentrations increased from 0 to 200 mg·L–1 ethephon. Overall, ethephon substrate drench efficacy was not reduced by the incorporation of perlite, pine bark, or wood fiber in peat-based substrates.
Speakers
WR

William Rich

The Ohio State University
Co-authors
GO

Garrett Owen

The Ohio State University
Thursday July 31, 2025 12:00pm - 12:15pm CDT
Foster 2

12:15pm CDT

PGR - Evaluating the Effect of PEG-Induced Drought Stress on Seed Germination in Ornamental Plant Species
Thursday July 31, 2025 12:15pm - 12:30pm CDT
Polyethylene glycol (PEG) is widely used to simulate drought stress in plant germination studies due to its ability to induce osmotic stress without being absorbed by plant tissues. This study investigates the effects of PEG-induced drought stress on the seed germination of various ornamental plant species, including Celosia argentea, Petunia hybrida, Rudbeckia hirta, Salvia splendens, and Zinnia elegans. Seeds are treated with different concentrations of PEG-6000 (0%, 5%, 10%, and 15%) to mimic varying levels of water deficit. Germination trials are conducted under controlled environmental conditions using LED lights with a 16/8-hour photoperiod to simulate light/dark conditions, and an optimum temperature of 70-75°F (21-23°C) to support ideal germination and seedling growth. Current seed research trials are underway with aims to evaluate germination-related traits under stress, including germination percentage, mean germination time, germination rate, and seedling vigor. These traits are used to assess the early-stage drought tolerance of ornamental plants. Understanding how different species respond to water stress at the germination stage is crucial for the ornamental plant industry, as it supports the selection and development of more resilient varieties suited for landscapes and markets increasingly affected by water scarcity.
Speakers
HG

Haramrit Gill

University of California Agriculture and Natural Resources
NA
Co-authors
SB

Santosh Bhandari

University of California Agriculture and Natural Resources
NA
Thursday July 31, 2025 12:15pm - 12:30pm CDT
Foster 2
  Oral presentation, Plant Growth Regulation

1:44pm CDT

GG 2 - Oral Session
Thursday July 31, 2025 1:44pm - 1:45pm CDT
Presiding/Moderator
SY

Shengrui Yao

New Mexico State University
Thursday July 31, 2025 1:44pm - 1:45pm CDT
Foster 2

1:45pm CDT

GG 2 - Jujube germplasm metabolomic study of fruit and seeds in New Mexico and western Texas
Thursday July 31, 2025 1:45pm - 2:00pm CDT
To fully evaluate jujube germplasm, we sampled sour jujubes both from Las Cruces, NM and western Texas to examine their fruit and seed metabolomic profiles to facilitate further employment of those jujube germplasm trees. Samples were taken from the NMSU campus and Tornillo/Fabens, TX which had both the wild type and middle types (cross between wild ones and cultivars). Jujube germplasm fruit metabolomic profile reveals that jujube cultivar samples were similar to germplasm samples from Texas. Sour jujube samples in NM were separated from sour jujube from TX. Sour jujube in TX were mingled together with Cross in TX. So-called Cross and sour jujube were arbitrary classifications. Without cultivars, germplasm was separated by location NM vs TX, not by sour jujube or Cross. For significant compounds, there were only 110 significant different compounds between TX sour jujube vs Cross, while Cross vs NM sour jujube, TX sour jujube vs NM sour jujube or TX vs NM, had over 700. TXS and Cross group overlaid and NM group was totally separated from the other two groups. TX samples had significantly higher contents of large numbers of amino acids and derivatives. More compounds were identified from seed samples and their grouping/PCA results were similar to fruit metabolomic results. Cross samples were mixed together with TX sour jujubes and NM sour jujubes were separated from TX samples. New Mexico samples in Las Crues near graduate student housing area were planted at similar time which could be from one nursery, closed related to each other. Texas germplasm was the result of human selection, not the original sour jujubes but cross between sour jujubes or sour jujube and cultivar-like germplasm. The dominant triterpenes were different between fruit and seeds. In fruit flesh, pomolic acid was the dominant one with Honeyjar as the highest, followed by rutundic acid, Cleanothic acid, 2,3,23-Trihydroxyurs-12-en-28-oic acid, 2,3,23-Trihydroxyolean-12-en-28-oic acid, madasiatic acid, which were higher for NM samples than TX samples. In seeds, the dominant triterpenes were oleanolic acid, mangiferotic acid, momordicoside I aglycone, 3,13,15-trihydroxyolenonane-12-one, jujubogenin, and pomolic acid. The contents of the first three metabolites were equivalent and much higher than the rest, ranging from 0.5X108 to 1.5x108 depending on germplasm. Pomolic acid was much lower in seeds than in fruit. The data contained over 1600 metabolites in fruit and over 2000 for seeds which would be good references for future utilization of those jujube germplasm for horticultural or pharmaceutical purposes.
Speakers
SY

Shengrui Yao

New Mexico State University
Thursday July 31, 2025 1:45pm - 2:00pm CDT
Foster 2

2:00pm CDT

GG 2 - Unraveling the Genetic Architecture of Key Traits for Prunus Crop Improvement
Thursday July 31, 2025 2:00pm - 2:15pm CDT
The accumulated genetic, genomic, and breeding data for Prunus species is often underutilized in breeding applications. This study examines 25 years of curated Prunus data in the Genome Database for Rosaceae (GDR, rosaceae.org) to uncover the genetic architecture of key traits, and provide actionable insights for Prunus breeding. The curated dataset includes 177 genetic maps, primarily for almond, apricot, peach, and sweet cherry, and 28,971 trait-associated loci. Most of the trait associations (72.4%) were from genome-wide association studies (GWAS), 18.7% from quantitative trait loci (QTL), and 8.9% from Mendelian trait loci. We identified 17 potential QTL hotspots for fruit morphology, fruit quality, and disease resistance, as well as 17 syntenic regions among peach, sweet cherry, and almond. These findings provide valuable resources for tool development for Prunus breeding, particularly for complex polyploid genomes and less-studied species.
Speakers
avatar for Michael Itam

Michael Itam

Clemson University
Co-authors
CC

Chun-Haui Cheng

Washington State University
NA
DM

Dorrie Main

Washington State University
KB

Katheryn Buble

Washington State University
NA
KG

Ksenija Gasic

Clemson University
PZ

Ping Zheng

Washington State University
NA
SJ

Sook Jung

Washington State University
NA
TL

Taien Lee

Washington State University
NA
Thursday July 31, 2025 2:00pm - 2:15pm CDT
Foster 2

2:15pm CDT

GG 2 - Pyrus Pangenome Enables Identification of Genes Involved in Adaptation to Niche Environments
Thursday July 31, 2025 2:15pm - 2:30pm CDT
Pears (genus Pyrus) are one of the most widely cultivated temperate fruit. Both abiotic and biotic stress, however, can be harsh constraints on pear cultivation; in America pear production has nearly ceased in the Eastern half of the nation, and in Europe extreme weather has become a growing threat to production, especially in Southern growing regions. Currently, high-quality reference genomes exist for the most widely cultivated Pyrus species, but little genomic information is available on ornamental, less cultivated, and wild Pyrus species. These species inhabit a wide range of climates across Eurasia, exhibiting diverse physiological adaptations to disease, high temperature, and water stress, while also showing variation in fruiting physiology and tree architecture. Discovery of genomic features responsible for this wide functional diversity could be applied to accelerating the genetic improvement of commercially cultivated Pyrus species. In order to characterize the genetic diversity within Pyrus, Nanopore whole genome DNA sequencing has been completed on 24 Pyrus accessions collected from the National Clonal Germplasm Repository, enabling highly contiguous (median N50 ~30Mb) and complete (median ~99% BUSCO assessed completeness), telomere-to-telomere assemblies with Hifiasm. Ab initio gene prediction via the BRAKER pipeline followed by comparative analysis with OrthoFinder has been used to find biome specific genes, while synteny analysis via MCScanX allows for the exploration of structural alterations in the evolution of Pyrus. These newly characterized Pyrus accessions represent an expansion of genomic resources to aid in the development of more resilient pears for the future.
Speakers Co-authors
AD

Amit Dhingra

Texas A
NA
KE

Kate Evans

Washington State University
NA
Thursday July 31, 2025 2:15pm - 2:30pm CDT
Foster 2

2:30pm CDT

GG 2 - Analysis of Genomic Variation through Whole-Genome Sequencing in Korean Peach Genetic Resources
Thursday July 31, 2025 2:30pm - 2:45pm CDT
Peach (Prunus persica (L.) Batsch) is a member of the genus Prunus within the Rosaceae family and represents one of the most extensively cultivated temperate deciduous fruit crops, ranking after apples and pears in global production. Due to its diploid genome (2n = 16) and relatively small genome size (~230 Mb), peach serves as a model species for fruit tree genome research. In this study, we performed whole-genome sequencing (WGS) on 445 peach genetic resources using the Illumina NovaSeq 6000 platform at a sequencing depth of 15´ coverage. Single nucleotide polymorphisms (SNPs) were identified from the WGS data and used to establish a core collection of peach genetic resources. Additionally, these SNPs will be utilized in a genome-wide association study (GWAS) to investigate key agronomic traits, including fruit shape, pollen fertility, flower morphology, maturity timing and so on. SNP filtration was conducted based on the following criteria: (1) SNPs with a missing rate exceeding 30% were removed, and (2) SNPs with a minor allele frequency (MAF) below 0.05 were excluded. As a result, 944,670 high-confidence SNPs were identified across the peach genetic resources. Based on this dataset, we established a core collection consisting of 150 accessions that retained over 99% of the total genetic diversity observed within the 445 peach genetic resources. Furthermore, we developed a high-resolution melting (HRM) marker derived from WGS-identified SNPs, which enables differentiation between round and flat peach fruit shapes. The SNP regions that can distinguish the fruit shape (round and flat shape) identified in this study were confirmed to be the same regions as the results of previously reported papers. Collectively, we successfully constructed a peach core collection through WGS analysis and developed a HRM marker for fruit shape classification. Also, our results produced in this study should be valuable for peach breeding program, identifying of agriculturally important genes, GWAS analyses, and further genomic studies in peach.
Speakers
NJ

Namhee Jeong

National Institute of Horticultural and Herbal Science, RDA
Thursday July 31, 2025 2:30pm - 2:45pm CDT
Foster 2

2:45pm CDT

GG 2 - Population Structure Analysis in Peach and Development of KASP Markers using SNPs generated by Capture-Seq technology
Thursday July 31, 2025 2:45pm - 3:00pm CDT
Although peach production worldwide has been increasing for decades, peach production in the United States continues to decline in the face of changing climate, disease pressures, and reduced consumption. Novel and diverse germplasm is required to improve peach breeding efforts with the goal of developing new cultivars better adapted to these challenges. Unfortunately, current peach SNP genotyping platforms are expensive and need to be outsourced to specialized laboratories. The purpose of this project is to use SNPs generated using Capture-Seq technology to evaluate the diversity of potential new sources of breeding material in comparison with germplasm from different regions of the world. In addition, our goal is to create a panel of SNP-based markers that can be used in-house for future studies. Capture-Seq technology yielded 134,424 SNPs when comparing P. persica (221 genotypes) and related Prunus species (29 genotypes). A PCA from these SNPs yielded different clusters representing Asian, Australian, European, and North American germplasm. AMOVA indicated that, among P. persica samples, 21.3% of the genetic variation was between regions with 78.7% of the variation present within regions. STRUCTURE analysis showed differences between regional groups, where the Asian group composition was different to the other regions, North American and European group composition were similar to each other, and the Australian group composition had a large percentage of genotypes sharing a group mostly present in Asia. This study confirms that Australia’s peach populations could be a valuable source of novel germplasm to bolster worldwide peach breeding efforts. Furthermore, a panel of informative SNP markers can be converted into KASP markers, which can be used in-house for numerous applications, including genetic fingerprinting, MAS, GWAS, among others.
Speakers
CM

Caitlin McCann

University of Georgia
Co-authors
BT

Bruce Topp

University of Queensland
NA
DC

Dario Chavez

University of Georgia
NA
DR

Dougal Russel

Queensland Government
NA
JC

Jose Chaparro

University of Florida
NA
RI

Rachel Itle

University of Georgia
NA
Thursday July 31, 2025 2:45pm - 3:00pm CDT
Foster 2

3:00pm CDT

GG 2 - Unlocking the Citrus Code: 'Hamlin' Genome Sequencing for Breeding Program Advancement in Florida
Thursday July 31, 2025 3:00pm - 3:15pm CDT
‘Hamlin 1-4-1’ sweet orange (Citrus sinensis L. Osbeck) is one of the major varieties cultivated in Florida and is of relevant importance for the orange juice industry as an early maturing variety. While this cultivar does not produce juice of sufficient quality to meet USDA Grade A orange juice standards, it performs relatively well in semitropical climates characterized by high temperatures and humidity levels. To provide the bioinformatics tools required to support the genetic improvement of modern citrus varieties, we present the de novo and fully phased ‘Hamlin’ genome. The DNA of the plant was sequenced using two different platforms. PacBio technology was adopted to generate long reads sequencing, while Oxford Nanopore was employed to produce ultra-long reads. Hi-C technique was used to capture chromosome conformation and facilitate the correct assembly of contigs into two haplotypes. RNA samples were collected from five different tissues (leaves, petals, ovaries, peel, and bark) and sequenced with the Illumina platform. These RNA sequences enabled the identification and annotation of as many functional genes as possible. The results of this study will provide the genomic information required to compare the ‘Hamlin 1-4-1 genome with the more commonly grown industry standard ‘Valencia’ and to investigate the differences between the genomes of these two clonally derived sweet oranges. These data will also aid in comparing budlines of Hamlin and other sweet orange accessions that appear to be HLB tolerant. This research will facilitate the detection of DNA variants related to traits of interest and their integration in new germplasm resources. In addition, it will allow breeders to get further insights into mutations that may have occurred to new budlines originating from ‘Hamlin’.
Speakers
GL

Giuseppe Lana

University of Florida
Co-authors
FJ

Frederick Jr. Gmitter

University of Florida
NA
JC

John Chater

University of Florida
Thursday July 31, 2025 3:00pm - 3:15pm CDT
Foster 2

3:15pm CDT

GG 2 - Investigating patterns of aneuploidy in potatoes using the R Package Qploidy.
Thursday July 31, 2025 3:15pm - 3:30pm CDT
Aneuploidy refers to a condition in which a cell or organism that has an abnormal chromosome number compared to the base chromosome number. This can cause gene dosage imbalances and a potential decrease in fitness. Most potato (Solanum tuberosum) cultivars are tetraploid (2n=4x=48) with a base chromosome number of 12. In this study, we analyzed 1,014 potato genotypes, 422 from two autotetraploid bi-parental full-sib populations and 592 from a diversity panel. We used allele SNP fluorescent intensity data for each individual to determine their ploidy and identify aneuploid individuals using the R package Qploidy. This package estimates the copy number by evaluating the standardized B allele frequencies (BAF) distributions across a sample, chromosome, or chromosome arm. Within the mapping populations, 41% of the members were aneuploids, compared to only 17% of the in the diversity panel; with an average of 27% aneuploidy level across all individuals included in the study. However, the frequency of aneuploidy for any given chromosome was 3%. As a measure of fitness, we compared 19 phenotypic traits related to tuber yield and quality in one of the full-sib mapping populations. There were significant differences between aneuploid and euploid family members for six traits. Aneuploid genotypes had significantly lower total tuber weight/plant, marketable tuber weight/plant, non-marketable tuber number/plant, tuber density, and overall appearance, while having higher percentage of tubers with heat sprouts compared to euploid family members. Chromosome additions were more common than chromosome losses in aneuploid individuals accounting for 57% and 39% of the aneuploid chromosomes, respectively. By analyzing this large potato genotypic dataset (most autotetraploids), we gained a better understanding of patterns of aneuploidy and their impact on crop performance in polyploid crops.
Speakers Co-authors
Thursday July 31, 2025 3:15pm - 3:30pm CDT
Foster 2

3:59pm CDT

PLAST - Oral Session
Thursday July 31, 2025 3:59pm - 4:00pm CDT
Presiding/Moderator
avatar for Jianying Wang

Jianying Wang

Washington State University
Thursday July 31, 2025 3:59pm - 4:00pm CDT
Foster 2

4:00pm CDT

PLAST - Evaluation of Mulch Types on Yield and Quality of Tomato and Lettuce
Thursday July 31, 2025 4:00pm - 4:15pm CDT
High tunnels (HT) are utilized by vegetable growers to extend the growing season and improve the yield and quality of crops. However, concerns about the sustainability of HT soil health can have direct effects on yield and profitability. Recently, growers and researchers have expressed concerns related to soil moisture in high tunnels and potential negative consequences to soil health. The use of non-permeable polyethylene tarps has become popular for urban and small-scale vegetable farmers to manage weeds through occultation. Similar tarps could also be used as mulch, but little is known about how this will affect crop productivity compared to other mulch types. The goal of this study was to examine silage tarps and other mulch types in HTs and the open field to determine how this practice may affect yield and quality of tomato and lettuce. Tomato and lettuce trials were conducted from 2022 to 2024 in HTs and open-field plots to determine the effect of various mulch treatments (bareground, black plastic film, black woven fabric, paper mulch, white silage tarp and black silage tarp) on tomato and lettuce yield and quality. For tomato, harvesting occurred weekly to monitor total, marketable, and non-marketable yield. Texture and organoleptic quality were assessed from 3 harvests across the two years. In 2023, lettuce were harvested and yield data were collected. Leaf color and water content were also assessed. In the HT tomato trials, mulch had the greatest effects on yield during the early season and the application of white silage tarp and plastic film mulch increased early marketable fruit yield by 58% compared to the bareground treatment. Mulch type had no significant effect on tomato or lettuce on the postharvest qualities that were assessed. Our data indicate that silage tarps may be an effective mulch, particularly for HT growers that are looking to conserve soil moisture and ultimately improve soil health. Yield and quality of tomato and lettuce were not penalized when tarps were utilized, and in some cases, yield was improved. As growers continue to adopt practices that help sustain or improve soil health, it is likely that silage tarps and other mulch types can be effective at maintaining crop productivity
Speakers
CB

Claire Barnhart

Kansas State University
Co-authors
CR

Cary Rivard

Kansas State University
EP

Eleni Pliakoni

Kansas State University
PT

Peter Tomlinson

Kansas State University
NA
Thursday July 31, 2025 4:00pm - 4:15pm CDT
Foster 2
  Oral presentation, Plasticulture
  • Subject Plasticulture
  • Poster # csv
  • Funding Source K-State Research and Extension Center for Sustainable Agriculture and Alternative Crops 2023 and Kansas State University GRIP Project

4:15pm CDT

PLAST - Mulch Technologies to Reduce Agriculture Plastic Waste Generation in Strawberry Cultivation
Thursday July 31, 2025 4:15pm - 4:30pm CDT
Polyethylene (PE) mulch is a valuable tool that suppresses weeds, optimizes the soil and canopy microclimate, and enhances efficient production of quality fruits and vegetables. However, PE mulch is not biodegradable and limited cost-effective waste management options lead to large amounts of used PE mulch being annually landfilled, stockpiled on farms, burned, and sometimes buried in soil. The objective of this presentation is to review the status of alternative mulch technologies that have the potential to reduce plastic waste generation in strawberry (Fragaria × ananassa) cultivation with an emphasis on soil-biodegradable plastic mulch (BDM), hydromulch, and cellulose-based film. Several commercial BDM products have been available in the market since the 1990s and are made using a blend of fossil-fuel derived and biobased ingredients. Trials conducted in Washington State show strawberry yield and fruit quality are comparable when plants are grown with black PE mulch or BDM. Green BDM deteriorates rapidly depending on environmental conditions. Rapid deterioration may lead to increased weed pressure and reduced production similar to bare ground cultivation. Hydromulch is a sprayable mulch alternative that can be formulated with ingredients that meet the requirements for certified organic production in North America, whereas commercially available BDMs do not meet these requirements. Trials carried out in Washington State and North Dakota demonstrated hydromulch maintains strawberry yield and fruit quality. Hydromulch formulations with guar gum demonstrate superior mechanical properties relative to formulations without or with other tackifiers. Reduced weed suppression, the logistics of sourcing hydromulch feedstock, lack of specialized application equipment, and high material and application costs are current barriers to this emerging technology. Cellulose-based film, such as lignocellulose film, is another fully biodegradable and emerging alternative to PE mulch and can be made with ingredients suitable for certified organic production. Field trials with cellulose-based film are limited, but current findings show high levels of biodegradability and maintenance of crop growth. Growers, crop consultants, and marketers should consider biodegradable mulch alternatives to reduce plastic waste generation and persistent plastic pollution in agricultural and environmental settings, particularly if recycling is not available or a cost-effective option.
Speakers
MG

Makonya Givemore Munashe

Washington State University
Co-authors
AW

Aidan Williams

Washington State University
AD

Andrew Durado

Montana State University
NA
BW

Ben Weiss

Washington State University
Ben is from Philadelphia and has been interested in food systems since an early age. He attended his first natural products expo before kindergarten because his parents worked in the natural foods industry. During late adolescence, he started a prolific tomato garden that blossomed... Read More →
CM

Carol Miles

Washington State University
DG

Deirdre Griffin LaHue

Washington State University
NA
DB

Dilpreet Bajwa

Montana State University
NA
GG

Greta Gramig

North Dakota State University
NA
HD

Haishun Du

University of Wisconsin-Madison
NA
LW

Lisa Wasko DeVetter

Washington State University
Lisa Wasko DeVetter joined Washington State University in 2014 and leads the small fruit horticulture program at the Northwestern Washington Research and Extension Center in Mount Vernon, Washington. She has developed a diverse research and extension program with an emphasis on maximizing... Read More →
NS

Nataliya Shcherbatyuk

Washington State University
NA
NG

Nayab Gull

Washington State University
Nayab is a Ph.D. student in the crop and soil science department Washington state University. She received her masters degree in Crop Cultivation and Farming Systems from China Agricultural University.
SP

Suzette Pedroso Galinato

Washington State University
NA
WA

Waqas Ahmad

North Dakota State University
NA
XP

Xuejun Pan

University of Wisconsin-Madison
NA
Thursday July 31, 2025 4:15pm - 4:30pm CDT
Foster 2
  Oral presentation, Plasticulture
  • Subject Plasticulture
  • Poster # csv
  • Funding Source Funding was provided by the USDA Specialty Crops Research Initiative Award 2022-51181-38325, Agriculture and Food Research Initiative Award 2023-68016-38933, and Organic Research and Extension Initiative 2021-51300-34909 from the USDA National Institute of Food and Agriculture. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. Additional support was provided by NIFA Hatch projects 7003737, 7001317, and W5188 Multi-State Project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA.
  • Funding Option SCRI funded all or part of the research associated with this abstract

4:30pm CDT

PLAST - Increased Profitability and Sustainability in Vegetable Production through Plastic Mulch Management
Thursday July 31, 2025 4:30pm - 4:45pm CDT
An estimated two million tons of plastic mulch films (PMFs) are used in horticultural production worldwide due to the benefits these films provide that may lead to yield increases. Despite the high usage rates of PMFs, there are many environmental drawbacks to the implementation of plastic mulches. To investigate potential mitigation strategies, two plastic and two biodegradable plastic films were used for multiple consecutive growing seasons. Soil conditions, plant yield and mulch durability were used to evaluate the soundness of reusing mulch films for multiple growing seasons. There were no significant differences between mulch types for the first season, with the highest yield being 307.23 pounds of tomatoes and the lowest yield being 235.62 pounds of tomatoes per 50 row feet. There were significant yield differences between mulch types for the second season, where each cabbage head produced on plastic mulches weighed an average of 3.68 pounds while each cabbage head produced on biodegradable mulches weighed between 1.22 and 1.84 pounds. The yield differences observed in season two may be in part due to increased soil penetration resistance under the biodegradable mulches, which had to be replaced at the end of the first season. The biodegradable mulches had to be replaced at the end of the first season due to their extreme degradation, but they were replaced at unfavorably high soil moisture conditions which led to considerable compaction, and higher penetration resistance, in those plots. Biodegradable mulched plots exhibited more than 50% bare ground at the end of the first growing season while plastic mulched plots both exhibited less than 10% bare ground. In addition to their in-field degradation, biodegradable films are also significantly weaker materials than PMFs, with average tensile strengths of 2.02 N and 2.91 N at the end of season one, compared to the average tensile strength of 4.56 N of conventional polyethylene. These results suggest that biodegradable PMFs may not be a good option for use over multiple seasons. Further research is needed to determine the optimum management practices for increased profitability PMFs in vegetable production systems while also improving environmental friendliness.
Speakers
MH

Melanie Hill

Auburn University
Co-authors
PB

Paul Bartley

Auburn University
NA
RH

Robert Harriman

The Ohio State University
NA
WF

Wheeler Foshee

Auburn University
NA
Thursday July 31, 2025 4:30pm - 4:45pm CDT
Foster 2

4:45pm CDT

PLAST - Life Cycle Assessment Insights into Environmental Trade-offs and End-of-Life Strategies for Soil-Biodegradable Mulch Films
Thursday July 31, 2025 4:45pm - 5:00pm CDT
Mulch films play a crucial role in enhancing crop yields and suppressing weeds; however, conventional plastic mulch films (PEMs) contribute significantly to environmental burdens, particularly at the end-of-life (EOL) stage. In response, soil-biodegradable mulch films (BDMs) have emerged as an alternative, offering potential benefits in reducing waste and emissions. This study conducts a cradle-to-grave life cycle assessment (LCA) of BDMs (PBAT/PLA, 30/70 PBAT/TPS, 70/30 PBAT/TPS blends) compared to PEMs (LDPE) across ten environmental impact categories in the production of 1 kg of strawberries per hectare in California, USA. Multiple EOL scenarios were evaluated, including soil-biodegradation, anaerobic digestion, and composting for BDMs, and landfill (with and without energy recovery) for PEMs. Results indicated that during the manufacturing stage, BDMs exhibited the lowest fossil fuel depletion, with the 30/70 PBAT/PLA achieving a 72% reduction; however, all BDMs had higher global warming potential (GWP). The highest environmental burdens in manufacturing were associated with BDM-PBAT/PLA due to its high energy requirements. During the mulch use stage, all BDMs consistently outperformed PEMs slightly in key impact categories such as GWP (reducing emissions by approximately 4.7%), acidification, and smog formation. No significant differences were observed among BDMs in this stage, suggesting that primary environmental distinctions arise from manufacturing and EOL scenarios rather than field application. The EOL stage significantly influenced the overall sustainability of mulch films. In terms of fossil fuel consumption, carbon emissions, and human toxicity potential, the most favorable scenarios were BDM-soil-biodegradation (-2.65 kg CO2-eq) and BDM-anaerobic digestion (-20.9 kg CO2-eq), both of which also reduced ecotoxicity (approximately -51 CTUe) and carcinogenic effects while minimizing fossil fuel depletion (approximately -1.72 MJ). In contrast, BDM-composting resulted in higher acidification (up to 0.344 kg SO2 eq) and smog formation, making it a less favorable option. PEMs, even under energy recovery scenarios, exhibited higher impacts and lacked the benefits of biodegradability, emphasizing the sustainability advantages of BDMs. When considering the full cradle-to-grave life cycle, BDMs demonstrated superior environmental performance under optimal EOL strategies. Although PEMs had a lower manufacturing footprint, their EOL challenges negated these benefits. Among BDMs, PBAT/TPS blends, particularly the 30/70 PBAT/TPS, exhibited the most balanced performance, offering reduced manufacturing burdens alongside excellent EOL outcomes. This study provides a detailed analysis of the environmental benefits and trade-offs of BDMs. The findings, along with nuanced recommendations, support the transition toward more sustainable mulch film applications.
Speakers
avatar for Oluwatunmise Israel Dada

Oluwatunmise Israel Dada

Graduate Research Assistant, Washington State University
A Ph.D. Candidate passionate about the natural environment and sustainable solutions.
Co-authors
LY

Liang Yu

Kentucky State University
NA
TC

Ting Chi

Washington State University
Thursday July 31, 2025 4:45pm - 5:00pm CDT
Foster 2
  Oral presentation, Plasticulture
  • Subject Plasticulture
  • Poster # csv
  • Funding Source USDA National Institute of Food and Agriculture, Specialty Crops Research Initiative Award 2022-51181-38325
  • Funding Option SCRI funded all or part of the research associated with this abstract

5:00pm CDT

PLAST - Beyond Plastic: Biobased Mulch Maintains Plant Growth with Rapid Degradation
Thursday July 31, 2025 5:00pm - 5:15pm CDT
Lignocellulosic film is a biodegradable alternative to traditional, non-biodegradable polyethylene (PE) mulch, and is made with fully biobased feedstocks. The biobased composition of lignocellulose film renders it suitable for certified organic production in the United States and Canada, whereas soil-biodegradable plastic mulches are not permitted given they are not made with fully biobased feedstocks. However, information regarding the horticultural performance of lignocellulose film as a mulch is lacking. The objective of this study was to address this knowledge gap by comparing the functionality of lignocellulosic film to other mulch treatments using raspberry grown in a greenhouse. ‘Cascade Premier’ tissue culture transplants were planted in 12.7 x 12.7 cm (1.89 L) pots and grown for 112 days. Mulch treatments were placed over the surface of the media and around the base of the plants before being arranged in a randomized complete block design with eight replicates. Treatments included PE, soil-biodegradable mulch (starch-based, PBAT copolyester), paper mulch, lignocellulosic film, lignocellulosic film with biochar, cellulose film, and no mulch. Mulch treatment did not significantly impact overall plant growth throughout the trial, although variations in soil temperature were observed with lignocellulosic film having the highest reported temperature overall. Following the greenhouse experiment, new mulch samples were subjected to a soil biodegradation assay whereby samples were buried in two contrasting climates in Washington (i.e., warm-summer Mediterranean and cold semi-arid climates) with four replicates per location. Lignocellulosic film made with a hot press and lignocellulosic film with biochar and a hot press were added as additional treatments. Mesh bags containing the mulch samples were collected and analyzed for visual breakdown. Differences in breakdown were observed across mulch treatments with cellulose film being the most degraded, PE mulch being the least degraded, and lignocellulose being intermediate. After 3 months, lignocellulose had degraded by approximately 50% in both locations. Results to date indicate films made with lignocellulose maintain plant growth relative to PE mulch and biodegrade rapidly once incorporated into the soil. Future work should focus on scaling up lignocellulose material generation so they may be evaluated in open-field settings.
Speakers
AW

Aidan Williams

Washington State University
Co-authors
HD

Haishun Du

University of Wisconsin-Madison
NA
LW

Lisa Wasko DeVetter

Washington State University
Lisa Wasko DeVetter joined Washington State University in 2014 and leads the small fruit horticulture program at the Northwestern Washington Research and Extension Center in Mount Vernon, Washington. She has developed a diverse research and extension program with an emphasis on maximizing... Read More →
TL

Teng Li

University of Maryland
NA
XP

Xuejun Pan

University of Wisconsin-Madison
NA
YY

Yuan Yao

Yale University
NA
Thursday July 31, 2025 5:00pm - 5:15pm CDT
Foster 2
  Oral presentation, Plasticulture

5:15pm CDT

PLAST - Beyond Disposal: A Comparative Life Cycle Assessment of End-of-Life Strategies for Agricultural Plastic Mulch
Thursday July 31, 2025 5:15pm - 5:30pm CDT
While agricultural plastic mulch significantly enhances crop yields, its widespread use generates substantial plastic waste, raising serious environmental concerns. Traditional disposal methods such as landfilling and incineration not only contribute to greenhouse gas emissions but also result in valuable resource losses. To address these challenges, this study performs a systematic life cycle assessment (LCA) comparing five end-of-life (EOL) strategies for polyethylene (PE) mulch films in strawberry cultivation: conventional landfilling, incineration, pyrolysis-based conversion, wood-plastic composite (WPC) production, and asphalt modification. A gate-to-gate LCA framework was adopted, with system boundaries spanning from on-farm mulch collection to final material or energy recovery. Environmental impacts were evaluated using the TRACI 2.1 methodology, encompassing global warming potential (GWP), cumulative energy demand, air/water pollution, land occupation, resource recovery efficiency, microplastic leakage risks, and human health impacts. Results reveal distinct trade-offs among the EOL pathways. Pyrolysis emerges as the optimal energy recovery strategy, reducing fossil fuel dependence by converting 85% of plastic waste into syngas while mitigating microplastic release. WPC production demonstrates superior material circularity, repurposing 92% of waste into durable construction materials with a 40-year service life. Asphalt modification offers the lowest GWP (1.2 kg CO₂-eq/kg plastic) and reduces virgin polymer demand by 30%, though its long-term microplastic leaching requires further investigation. In contrast, landfilling and incineration exhibit 60-75% higher life cycle emissions and fail to recover material value. This study provides the first comprehensive comparison of agricultural plastic waste management strategies that integrates both energy recovery and material upcycling paradigms. By extending traditional LCA boundaries to include microplastic pollution risks and long-term degradation effects, the findings offer actionable insights for policymakers to prioritize scalable, resource-efficient solutions that align with circular economy principles in agri-plastic management.
Speakers
avatar for Jianying Wang

Jianying Wang

Washington State University
Co-authors
LY

Liang Yu

Kentucky State University
NA
TC

Ting Chi

Washington State University
Thursday July 31, 2025 5:15pm - 5:30pm CDT
Foster 2
  Oral presentation, Plasticulture
  • Subject Plasticulture
  • Poster # csv
  • Funding Source USDA SCRI 2022-51181-38325
  • Funding Option SCRI funded all or part of the research associated with this abstract

5:30pm CDT

PLAST - Two-Year Evaluation of Biodegradable Plastic Mulches for Winter Strawberry Production in Florida: Mulch Deterioration, Plant Growth, Yield, and Fruit Quality
Thursday July 31, 2025 5:30pm - 5:45pm CDT
Biodegradable plastic mulches (BDMs) have been introduced as a sustainable alternative to polyethylene (PE) mulches, which can contribute to plastic contamination and incur high removal and disposal costs. The objective of this study was to evaluate the performance and suitability of BDMs for winter strawberry production in Florida. We conducted field experiments using ‘Florida Brilliance’ short-day strawberry during the 2023–2024 (Season 1) and 2024–2025 (Season 2) winter seasons in West Central Florida. Five mulch treatments were evaluated: black PE mulch, black Mater-Bi® BDM (black MB–BDM), black Ecovio® BDM (black EV–BDM), white Mater-Bi® BDM (white MB–BDM), and white Ecovio® BDM (white EV–BDM ). Mater-Bi® is primarily starch-based but also contains polyester resins such as polybutylene adipate terephthalate (PBAT) and polycaprolactone. By contrast, Ecovio® is predominantly resin-based, comprising PBAT, polylactic acid, and other biodegradable polymers. The film thickness was 27.9 μm for the black PE and 22.9 μm for all BDMs. Season-dependent trends were observed in some results. In Season 1, all BDMs exhibited minimal deterioration with only minor tearing, and there was no significant difference in marketable yield compared to the black PE. In Season 2, all BDMs developed splits or tears within a month after transplanting, with the black EV–BDM and both white BDMs showing higher deterioration rates than the black PE. Orthogonal contrast analysis was used to assess BDM performance based on color and composition. Compared to the black PE, marketable yield was reduced by 28% for the black BDMs (34.0 vs. 26.5 t·ha–1) and by 8% for the white BDMs (34.0 vs. 31.3 t·ha–1). The white BDMs produced 18% higher yield than the black BDMs (31.3 vs. 26.5 t·ha–1), whereas no significant difference was found between the MB–BDMs and the EV–BDMs (28.8 vs. 29.1 t·ha–1). Soluble solid content remained unaffected by mulch treatment across both seasons and all growth stages. These results suggest that the performance of BDMs depends on seasonal weather conditions, mulch color, and composition, presenting challenges for commercial adoption. Enhancing the reliability and adoption of BDMs may require optimization of color, material composition, and film thickness.
Speakers
avatar for Hadi Ghasemi

Hadi Ghasemi

PhD Graduate Assistant, University of Florida
🌱 Passionate about CEA. 🪻 Love saffron and have expertise in growing it. 🍓 Strawberry physiology and photosynthesis.
Co-authors
LW

Lisa Wasko DeVetter

Washington State University
Lisa Wasko DeVetter joined Washington State University in 2014 and leads the small fruit horticulture program at the Northwestern Washington Research and Extension Center in Mount Vernon, Washington. She has developed a diverse research and extension program with an emphasis on maximizing... Read More →
NB

Nathan Boyd

University of Florida
NA
SA

Shinsuke Agehara

University of Florida
Thursday July 31, 2025 5:30pm - 5:45pm CDT
Foster 2
 
Friday, August 1
 

7:59am CDT

HEMP - Oral Session
Friday August 1, 2025 7:59am - 8:00am CDT
Presiding/Moderator
BP

Brian Poel

Fluence
Friday August 1, 2025 7:59am - 8:00am CDT
Foster 2

8:00am CDT

HEMP - Differential effects of macro- and micronutrients on secondary metabolite production in drug-type (medical) cannabis
Friday August 1, 2025 8:00am - 8:15am CDT
The medical potential of cannabis (Cannabis sativa L.) is based on the complex chemical profile, comprising hundreds of secondary metabolites including cannabinoids, terpenoids and flavonoids. Cultivation conditions were demonstrated to affect plant development, function and production of secondary metabolites in cannabis. Understanding regulation of plant response to environmental conditions is key for development of optimal chemical profile for modern medicine. We have recently demonstrated sensitivity of the secondary metabolite profile in medical cannabis to mineral nutrition, with considerable responses to N, P, and K nutrition. Therefore, knowledge on the cannabis plant response to fertigation schemes is essential for the optimization of cultivation for production of high quality standardized material for the medical market, as well as for development of plant products containing specific desirable phytochemical profiles. In the talk, we will discuss our recent results concerning the potential of additional macronutrients and micronutrients to regulate plant development and the profile of active secondary metabolites in ‘drug-type’ medical cannabis. In pot experiments under controlled conditions, we demonstrated differential induction of changes in the cannabinoid and terpene profiles in ‘drug-type’ medical cannabis also by Ca, Mg, Zn and Mn. Furthermore, rate of uptake and deposition in the plants of individual macronutrients and micronutrients changes between the vegetative and the reproductive developmental stages, and along the reproductive phase.
Speakers
NB

Nirit Bernstein

Volcani Center
Friday August 1, 2025 8:00am - 8:15am CDT
Foster 2

8:15am CDT

HEMP - Evaluation of High and Low Red-content Lighting During the Vegetative Phase of Cannabis Production
Friday August 1, 2025 8:15am - 8:30am CDT
As a short-day crop, Cannabis sativa, benefits from early-phase cultivation under long days to increase vegetative growth before transitioning to a generative flower phase. Previously, this long-day or “veg” phase has occurred under ceramic metal-halide lamps due to their relative increased blue content compared to the spectra of high-pressure sodium lamps used during the short-day or “flower” phase. Increased relative blue light during young plant production is desired due to the promotion of plant compactness and root development. Due to the relative efficacy of red diodes to blue, there is a benefit to maximizing red content without affecting plant performance. While many spectral recommendations arise from research in leafy greens and ornamentals, cannabis is cultivated under intensities three-fold greater, 600 µmol·m-2·s-1 during the long-day phase to 200 µmol·m-2·s-1 in leafy greens. Therefore, due to the high light intensity, it may be possible to produce optimal young plant quality under relatively low blue content. To test this, three cannabis cultivars rooted for 14 days were transplanted into 2-gallon coco coir bags and grown under long days with spectral treatments for an additional 14 days before finishing in a 12-hour short-day common environment. During the long-day phase, plants were exposed to high (~80%) or low (~40%) red at an average intensity of 500 µmol·m-2·s-1. Plant height at transfer to short days was similar regardless of light treatment. Additionally, final plant height and total flower yield after transfer into short days in a common environment was also similar. Therefore, it is beneficial to cultivate cannabis plants during long days under a high-red spectrum to minimize lighting cost while avoiding any negative morphology effects.
Speakers
BP

Brian Poel

Fluence
Co-authors
DH

Dave Hawley

Fluence
NA
Friday August 1, 2025 8:15am - 8:30am CDT
Foster 2

8:30am CDT

HEMP - Can Induced Water-deficit Stress Act As a Catalyst For Maximizing Cannabis Sativa Production and Secondary Metabolite Levels?
Friday August 1, 2025 8:30am - 8:45am CDT
Cannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the Cannabaceae family and produces economically important secondary metabolites called cannabinoids. According to the literature, controlled or induced water-deficit stress can increase secondary metabolite concentration in some essential oil-producing plants. Therefore, induced water-deficit stress (DS) may be an effective technique to maximize cannabinoid yield. This study investigated how different frequencies of induced water-deficit stress during the flowering stage affect cannabinoid yield and cannabis development compared to well-irrigated controls. By exploring the optimal stress conditions conducive to maximizing cannabinoid production, our study aims to offer strategic insights to inform cultivation practices and optimize cannabinoid production. This research contributes to advancing our understanding of cannabis cultivation techniques and may ultimately enhance the efficiency and efficacy of cannabinoid production on a commercial scale. Our hypothesis posits that induced water-deficit stress enhances plant secondary metabolism by modulating physiological responses. The treatments consisted of four frequencies of water-deficit stress periods during the cannabis flowering stage: WS0 – no stress, WS1 – one period of water-deficit stress, WS2 – two periods of water-deficit stress, and WS3 – three periods of water-deficit stress. The experiment was conducted using clones of the Heidi cultivar, which were randomly placed in the controlled-environment growth units. Weekly plant parameters included plant height, stem diameter, NDVI, chlorophyll content, photosynthetic efficiency, and stomatal conductance. After-harvest parameters included biomass partitioning, yield mass, bucked biomass, trichome density, and cannabinoid and terpenes levels. This is an ongoing study, but the preliminary data analysis shows interesting results regarding higher trichome density with no penalty for bucked biomass in the treatments under one period of water-deficit stress (WS1).
Speakers
avatar for Jose Leme

Jose Leme

Assistant Professor, Southern Illinois University
The Cannabis Biology and Cultivation Systems lab focuses on areas of controlled environment agriculture, plant stress, secondary metabolites, nutraceutical/pharmaceutical biology, and growing practices. The research team aims to determine how to leverage environmental controls (light... Read More →
Co-authors
AA

Aldwin Anterola

Southern Illinois University
NA
DO

Dila Ozev

University of Bonn
NA
FD

Fardad Didaran

University of Tehran
NA
JW

Jennifer Weber

Southern Illinois University
NA
SS

Shiksha Sharma

Southern Illinois University
NA
Friday August 1, 2025 8:30am - 8:45am CDT
Foster 2

8:45am CDT

HEMP - Beyond Static Spectrum: The Promises and Pitfalls of Dynamic Lighting for Cannabis Cultivation
Friday August 1, 2025 8:45am - 9:00am CDT
Dynamic lighting has recently evolved from theoretical research to commercial viability. As the cannabis industry faces increasing margin pressure, optimization techniques that enhance product quality while maintaining cost efficiency are essential for cultivation. But is dynamic lighting a silver bullet for enhanced cannabis cultivation? In this session Dr. Hawley will present research on UV, blue, red, and far-red light as it pertains to cannabis inflorescence quality and yield. New results will be presented that will inform how growers light their crops to maintain or exceed their current quality and yield while improving their lighting efficacy by 15% and reducing their lighting operational costs by 19%.
Speakers
DH

David Hawley

Fluence
In his role as Fluence’s Principal Scientist, Dr. David Hawley steers the company’s global Cannabis Crop Sciences program, encompassing photobiological research, data analytics, and cultivation consultation. Fluence’s research has identified specific and diverse lighting strategies... Read More →
Friday August 1, 2025 8:45am - 9:00am CDT
Foster 2

9:59am CDT

POM 3 - Oral Session
Friday August 1, 2025 9:59am - 10:00am CDT
Presiding/Moderator
FN

Franz Niederholzer

University of California Cooperative Extension
Friday August 1, 2025 9:59am - 10:00am CDT
Foster 2

10:00am CDT

POM 3 - Phosphorus Uptake and Partitioning in Young Peach Trees
Friday August 1, 2025 10:00am - 10:15am CDT
Phosphorus (P) is an essential plant nutrient required for growth. However, it is a finite natural resource that can cause environmental issues when overused in agriculture. Due to challenging soil relationships, the availability and uptake of P can be challenging to predict. Furthermore, soil tests can be misleading for guiding management decisions regarding P fertilizer applications. The objective of this study was to evaluate the influence of soil P levels on the uptake and partitioning of P in young peach trees. We planted a new peach orchard at the Musser Fruit Research Center near Clemson, SC. This orchard contained a widely variable range of P in the soil. Representative trees were removed in August and January and all plant parts were subjected to P analysis. Soil test P levels had no clear relationship with total plant P uptake or partitioning patterns and are likely a poor reference point for P fertilizer management decisions in peach orchards.
Speakers
avatar for Noah Willsea

Noah Willsea

Clemson University
Co-authors
JC

Juan Carlos Melgar

Clemson University
NA
Friday August 1, 2025 10:00am - 10:15am CDT
Foster 2
  Oral presentation, Pomology 3

10:15am CDT

POM 3 - Understanding the Role of Nutrient Leaching in Young Peach Trees: Implications for Growth and Development in Orchard Man
Friday August 1, 2025 10:15am - 10:30am CDT
Nutrient leaching remains a significant environmental concern due to its adverse effects on soil quality, groundwater contamination, human health, and the eutrophication of aquatic ecosystems. The urgency of addressing this issue has escalated with the increasing frequency and intensity of climate variability and extreme weather events, which exacerbate nutrient losses from agricultural systems. For this study, three peach cultivars (Prunus persica L.), including 'Crimson Joy', 'Liberty Joy', and 'Rich Joy', were planted at Auburn University's Research and Extension Center in Chilton, Clanton, Alabama, on February 23, 2024, using a Randomized Complete Block Design (RCBD) with four blocks. Each block consists of 9 peach trees, totaling 36 trees. The aim of this study is to evaluate the extent of nutrient leaching on the growth of three distinct young peach cultivars, using recommended fertilization rate (by The Southeastern peach guide). A soil sampling method is used to compare the nutrient levels at selected depths. The soil depths are 25.4 and 50.8 cm within and 76.2 and 101.6 cm below the peach tree root zone. The collected soil samples are analyzed for Nitrate Nitrogen (NO3-N (mg/kg)), Ammonium nitrogen (NH4-N (mg/kg)) and pH. Additionally, the young peach trees are assessed by measuring plant height (cm), trunk cross-sectional area (cm2) and annual leaf nitrogen analysis (%). The preliminary results of this study, which is still in progress indicate that both depth and cultivar significantly influence NH4-N concentrations over time, while rainfall events and cultivar interactions affect NO3-N leaching dynamics. Although pH levels changed, they did not differ statistically. Each cultivar exhibited TCSA increases and height growth, with all cultivars maintaining leaf nitrogen levels within the optimum range. This study will help the Alabama peach growers to understand the impacts of nutrient leaching and utilize better alternatives that can help to not only get maximum profit economically but also ensure that the environment is safe and sound for the current and future generations.
Speakers
WN

Winfred Nziku

Auburn University
Co-authors
AG

Audrey Gamble

Auburn University
NA
BC

Bernardo Chaves-Cordoba

Auburn University
NA
DC

Dario Chavez

University of Georgia
NA
EV

Edgar Vinson

Auburn University
JC

Juan Carlos Melgar

Clemson University
NA
MR

Melba Ruth Salazar Gutierrez

Auburn University
NA
Friday August 1, 2025 10:15am - 10:30am CDT
Foster 2
  Oral presentation, Pomology 3

10:30am CDT

POM 3 - Rapid and quantitative analysis of leaf calcium using X-ray fluorescence scanning
Friday August 1, 2025 10:30am - 10:45am CDT
Development of control measures for nutrient-related apple fruit disorders like bitter pit requires a better understanding of calcium metabolism in the tree. Limiting this understanding is a rapid and accurate method for measuring the concentration of calcium in various plant organs including the leaves. Optimally, measuring calcium levels could be done at the single leaf level, or better yet for whole leaves at a resolution of sub-millimeter dimensions. We report on the use of X-ray fluorescence for apple leaf tissue to quantitatively measure leaf calcium tissue. Using 7-mm diameter discs taken from Granny Smith apple leaves mid-season, we were able to demonstrate that the emission data from a scanning Bruker X-ray fluorescence instrument could be easily transformed to predict accurate and precise calcium levels in apple leaf tissue. If similar relationships can be determined for other minerals, this type of analysis will enable the quantitation of multiple minerals within minutes. Provided software analysis of the samples can be automated, the turn-around time for tissue analysis would be on the order of one or two hours for hundreds of samples
Speakers
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Co-authors
CM

Claudia Moggia

University of Talca
GL

Gustavo Lobos

University of Talca
MP

Marcelo Pirinoli

University of Talca
NA
RC

Ricardo Cabeza

University of Talca
NA
SR

Sebastian Romero-Bravo

University of Talca
NA
Friday August 1, 2025 10:30am - 10:45am CDT
Foster 2
  Oral presentation, Pomology 3
  • Subject Pomology
  • Poster # csv
  • Funding Source FONDECYT

10:45am CDT

POM 3 - Horticultural Performance and Physiological Responses of a Mature Peach Orchard to Mulch and Poultry Litter Application.
Friday August 1, 2025 10:45am - 11:00am CDT
Plant nutrient acquisition and responses to different fertilization programs in fruit orchards oftentimes depend on many biotic or abiotic factors or a combination of both, including tree or orchard age and nutrient source. This study aims to evaluate the effect of topically applied, slow-release source of essential nutrients from mulch mixed with poultry litter (PLM) or without (M) and fast-release growers’ standard rate of mineral fertilizer on an eight-year-old replanted orchard containing ‘Juneprince’ and ‘Scarletprince’ peach trees in South Carolina. Soil health indicators such as soil moisture and nutritional status, and tree health parameters, including yield and fruit quality (specifically on the incidence of diseases and physiological disorders), were evaluated over two years. The results showed that soil organic matter and cation exchange capacity were significantly higher in PLM across both years of this study when compared with other treatments. Both PLM and M treatments had higher moisture retention than the grower’s standard. However, yields between the two cultivars used in this study varied considerably in response to treatment and years. Yield of ‘Juneprince’ trees treated with GS was significantly higher in the first year of this study when compared with PLM and M but did not significantly differ in the second year. In contrast, yield of ‘Scarletprince’ trees treated with PLM and M were consistently higher numerically when compared with GS in both years. PLM significantly reduced bacterial spot incidence and severity in both cultivars across the study years. This result suggested that amending the soil through surface covering with mulch mixed with poultry litter provides the nutritional requirement, increases yield and significantly lower disease pressure and improves the quality of the marketable fruit in a mature orchard.
Speakers
AI

Ayodeji Idowu

Clemson University
I am a third-year Ph.D. student working on improving soil health and ecosystem conservation through rigorous enhancement of soil organic matter and strategic soil nutrient management for agricultural and environmental sustainability. To achieve this, I am testing different soil amendment... Read More →
Co-authors
JC

Juan Carlos Melgar

Clemson University
NA
Friday August 1, 2025 10:45am - 11:00am CDT
Foster 2
  Oral presentation, Pomology 3
  • Subject Pomology
  • Poster # csv
  • Funding Source United States Department of Agriculture - Sustainable Agriculture Research and Education (USDA-SARE)

11:00am CDT

POM 3 - Cutting Propagation of Southwest Peach Seedlings
Friday August 1, 2025 11:00am - 11:15am CDT
Southwest Native American Tribes, such as the Navajo, Hopi, and other Pueblo, have grown peaches since at least the early 1600’s, making them a nutritionally and culturally important food source. Historically, peach propagation was primarily done by seed, with only one written account of stem cutting propagation occurring within the Zuni Pueblo. Little is known about the effectiveness of different propagation methods for rapidly replicating Southwest seedling accessions and maintaining parent tree genetics. The purpose of this study was to evaluate the rooting success of softwood and hardwood cuttings taken from Southwest peach seedling accessions originating from five locations within the Navajo and Hopi Tribal communities. All cuttings were collected from mature trees established in Thatcher, UT. Two trials of cuttings were conducted in July and November of 2024. Each seedling tree had 18 cuttings per trial. Trial one had softwood and hardwood cuttings and trial two had only hardwood cuttings. Each cutting was measured 12 cm long, had at least three nodes planted into the potting media. Trial one cuttings had the first 3 node leaves cut in half, with all other leaves removed. Cutting treatments included a control (no treatment), Hormodin 1, and Hormodin 2, with six replications for each treatment. All cuttings were placed under a mist system with controlled temperature and relative humidity. Rooting success was assessed over three months by visually evaluating live tissue and new growth. Rooting success was dependent upon seedling parent location, treatment, time of year, as analyzed by analysis of variance. Cuttings from Canyon del Muerto had greater rooting success compared with those from Navajo Mountain. The Hopi and Navajo Mountain cuttings had decreased rooting success as the Hormodin concentrations increased. Canyon del Muerto cuttings benefited from Hormodin 1 treatment. The results of this study will help determine the potential of Southwest peach seedlings for replication as a viable rootstock for the fruit industry.
Speakers
RW

Reagan Wytsalucy

Utah State University
Native American food crops of the Southwest.
Co-authors
BL

Bud Lopez

New Mexico State University
NA
KL

Kevin Lombard

New Mexico State Univeristy
Kevin Lombard is a Professor of Horticulture and Research Director of the New Mexico State University Agricultural Science Center at Farmington, a 250 acre research farm uniquely located on the Navajo Nation. His research encompasses specialty crop evaluations including grapes, fruit... Read More →
YS

Youping Sun

Utah State University
Friday August 1, 2025 11:00am - 11:15am CDT
Foster 2
  Oral presentation, Pomology 3
  • Subject Pomology
  • Poster # csv
  • Funding Source San Juan County Extension with Utah State University and New Mexico State University Agriculture Science Center

11:15am CDT

POM 3 - Root-to-leaf ratio contributes to drought recovery and resilience in northern highbush blueberry genotypes
Friday August 1, 2025 11:15am - 11:30am CDT
Plant canopy and root architecture characteristics play a crucial role in water uptake and plant growth. Blueberry roots are shallow and lack dense root hairs, making them more susceptible to drought than most fruit crop species. In northern highbush blueberry (Vaccinium corymbosum), differences in root size, root architecture, and root-to-leaf balance may impact drought resilience, particularly in maintaining photosynthesis (A) under limited soil moisture. However, this has not yet been examined in this species. This study evaluated drought resilience in two V. corymbosum genotypes with contrasting canopy and root architecture characteristics (Bluecrop, low root-to-leaf ratio; Draper, high root-to-leaf ratio). During the pre-drying period, both genotypes exhibited similar A and stem water potential (Ψ stem). Irrigation water was withheld to initiate drought conditions. By day 4 of withholding water, Bluecrop showed a decline in A, but its Ψ stem did not significantly change until day 6. In contrast, Draper did not show a decline in A or Ψ stem until day 6 of withholding water. Both cultivars reached a critical threshold of stomatal conductance (gs) (90% reduction from well-watered conditions) on day 10 of withholding water. Following re-watering, Ψ stem recovered immediately in both cultivars on day 2 of re-watering (day 12 of withholding water). However, the recovered Ψ stem of Bluecrop did not correspond with any increase in gs until day 14 of rewatering (day 24 of withholding water). Meanwhile, Draper exhibited a much higher gs and A than Bluecrop throughout the post-rewatering period, suggesting that Bluecrop is more sensitive to drought conditions than Draper. Post-experiment analysis confirmed that Draper had a larger root-to-leaf ratio than Bluecrop, which likely enhanced water absorption and supported faster recovery following rehydration. No significant difference in root architecture characteristics were found between these two cultivars, considering the number of root tips, root length, and surface area per root diameter at different levels (< 0.25 mm, 0.25- 0.5 mm, and >0.5 mm). These results suggest that root-to-leaf ratio and root-to-leaf hydraulic conductance play a central role in determining drought resilience in V. corymbosum genotypes. Future studies will be carried out to confirm this finding across a greater number of genotypes.
Speakers
SL

Sheng-yang Li

Michigan State University
Co-authors
JV

Josh VanderWeide

Michigan State University
Friday August 1, 2025 11:15am - 11:30am CDT
Foster 2
  Oral presentation, Pomology 3

11:30am CDT

POM 3 - A review of progress and obstacles on a path towards consistent production of high quality ‘Improved French’ prune crop
Friday August 1, 2025 11:30am - 11:45am CDT
Consistent production (CP) of quality crops supports economic sustainability of individual farms and maintains market share for a larger industry. For the last 30 years, annual prune production in California has been highly variable, ranging from < 1 to >3 dried tons per acre. ‘Improved French’ is the primary cultivar grown in California. This variety, like most plums, often requires thinning to maintain CP and grower income where fruit size (count per pound) is positively correlated to fruit quality and price to the grower. Mechanical shaking at reference date, ~45 days after full bloom, is now a common thinning practice although questions have been raised regarding the potential benefit to earlier thinning a phantom practice that would have to be chemical thinning. However, in the last two decades, extreme bloom weather can virtually eliminate a prune crop if warm weather precedes bloom. This talk reviews work by University of California researchers towards improving CP through 1) better understanding of the weather related factors contributing to very low fruit set in prunes, 2) testing ‘Improved French’ prune fruit growth development to determine if and when source limited fruit growth occurs, and 3) identifying chemical thinning practices, materials, and rates to remove flowers before fruit set. Early bloom years are those with risk of crop loss due to extended and wet or extremely warm (>27oC) bloom weather conditions. [The risk of freezing damage is relatively low for prunes in CA given usual March bloom timing.] Relative prune fruit growth rate studies revealed resource limited fruit growth occurs ahead of current thinning timings (reference date), so earlier thinning may improve fruit growth and possibly yield compared to the current thinning practice. Finally, potassium thiosulfate (KTS) applied 2x at 1-2% (v/v) shows promise in thinning prunes. Lime sulfur and fish oil can be effective but is inconsistent in bloom thinning. Obstacles to CP of ‘Improved French’ prune include difficulty identifying practices to quantify bloom strength and help grower with the decision to thin or not. In addition, no ‘rescue’ spray has been found to improve fruit set in years with early, risky, bloom. Grower testing of the current research results awaits successful overcoming of the two obstacles listed above.
Speakers
FN

Franz Niederholzer

University of California Cooperative Extension
Co-authors
LM

Luke Milliron

University of California Agriculture Natural Resources
RR

Richard Rosecrance

California State University, Chico
NA
SC

Sara Castro

UC Davis
NA
TD

Ted DeJong

UC Davis
Friday August 1, 2025 11:30am - 11:45am CDT
Foster 2
  Oral presentation, Pomology 3
  • Subject Pomology
  • Poster # csv
  • Funding Source California Prune Board

11:59am CDT

QUAL - Oral Session
Friday August 1, 2025 11:59am - 12:00pm CDT
Presiding/Moderator
DL

Daniel Leiva

Research Assistant, Louisiana State University
Friday August 1, 2025 11:59am - 12:00pm CDT
Foster 2

12:00pm CDT

QUAL - First Season Crop Yield, Soil Chemical Properties, and Heavy Metal Accumulation Responses to Soil Amendments in Organically Grown Sweetpotato
Friday August 1, 2025 12:00pm - 12:15pm CDT
An on-farm field trial was conducted to investigate the feasibility of applying commonly used soil amendments to reduce the accumulation of arsenic (As), cadmium (Cd), and lead (Pb) in sweetpotato (Ipomoea batatas L.) storage roots. The cultivar ‘Beauregard’ was grown in an organically managed field with natural As, Cd, and Pb levels. The following soil amendments were applied: agricultural lime (AGL) (1 t·ac−1), gypsum (GYP) (1 t·ac−1), biochar (BIO) (1 t·ac−1), and silicon provided as wollastonite (WOL) (2.5 t·ac−1). Agricultural lime and WOL increased soil pH and calcium levels, ranging from 14% to 25% and 16% to 90%, respectively. Soil amendments were not associated with storage root yield variation. Wollastonite and BIO were associated with a trend for reduced As, Cd, and Pb in storage roots. These first-season study results suggest that WOL and BIO are potentially useful for follow-up studies to reduce the bioavailability and subsequent uptake of As, Cd, and Pb accumulation in sweetpotato under organic production systems.
Speakers
avatar for Mae Ann Bravo

Mae Ann Bravo

Graduate Student Assistant, Louisiana State University
Co-authors
AV

Arthur Villordon

LSU Agcenter
BT

Brenda Tubana

Louisiana State University
NA
DL

Don La Bonte

Louisiana State Univ
NA
LH

Lorin Harvey

Mississippi State University
NA
Friday August 1, 2025 12:00pm - 12:15pm CDT
Foster 2

12:15pm CDT

QUAL - Pathogen Species Causing Brown Rot of Peaches in Illinois and Efficacy of Fungicides for Managing the Disease
Friday August 1, 2025 12:15pm - 12:30pm CDT
Pathogen Species Causing Brown Rot of Peaches in Illinois and Efficacy of Fungicides for Managing the Disease Harrison Seitz1, Andrew N. Miller2, and Mohammad Babadoost1 1Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA; and 2Illinois Natural History Survey, University of Illinois, Champaign, IL 61820, USA Brown rot is one of the important diseases of peaches in Illinois. This study was conducted to: (i) identify pathogen species causing brown rot disease in commercial peach orchards in Illinois, and (ii) evaluate the efficacy of fungicides for managing the disease. Results of orchard surveys showed 9 of 14, 4 of 8, and 6 of 13 orchards had symptomatic brown rot fruits in 2020, 2021, and 2022, respectively. No blossom blight or shoot blight was observed. Symptomatic fruits were collected from eight peach cultivars throughout the state and the associated fungi were isolated. Based on the cultural characteristics and sequences of the ITS region, 127 of 129 collected isolates were identified as Monilinia fructicola, and two isolates as M. laxa. Fungicide sensitivity of M. fructicola isolates was conducted in the laboratory against azoxystrobin, captan, fenhexamid, trifloxystrobin, penthiopyrad, difenoconazole cyprodinil, fluopyram tebuconazole, fluopyram trifloxystrobin, fluxapyroxad pyraclostrobin, propiconazole, and thiophanate-methyl. The EC50 of azoxystrobin, captan, and penthiopyrad for the colony development of the isolates were significantly (P = 0.05) higher than the other fungicides tested. Field trials were conducted on ‘Redhaven’ and ‘Contender’ peaches for two years to evaluate efficacy of the commercial product of the above-mentioned fungicides. Trees were sprayed with fungicides at 10- and 14-day intervals. Luna Experience 3.34SC (fluopyram tebuconazole), Luna Sensation 4.20SC (fluopyram trifloxystrobin), and Merivon 4.18SC (fluxapyroxad pyraclostrobin) were the most effective fungicides for managing brown rot and other summer diseases of peach.
Speakers
MB

Mohammad Babadoost

University of Illinois
Co-authors
AM

Andrew Miller

University of Illinois
NA
HS

Harrison Seitz

University of Illinois
NA
Friday August 1, 2025 12:15pm - 12:30pm CDT
Foster 2

12:30pm CDT

QUAL - Harnessing Plasma-Activated Water: Impact of Processing and Storage Time on Pea Microgreen Productivity and Nutritional Quality
Friday August 1, 2025 12:30pm - 12:45pm CDT
Plasma-activated water (PAW) is a novel irrigation medium enriched with reactive oxygen and nitrogen species (RONS) which significantly modify the physiochemical properties of water, yet their inherent instability is influenced by processing parameters such as plasma exposure time and storage conditions. Although studies have demonstrated PAW’s promising effects on seed germination and plant growth, knowledge regarding its application to enhance microgreen production is limited. Therefore, the objective of this study was to elucidate the effects of PAW processing time and storage duration on the PAW chemical properties and on the yield and quality of pea (Pisum sativum L.) microgreens. Tap water was treated using a gliding arc plasma generator (200 mA, 2.6–2.7 kV) provided by Plasma Licensing Authority Inc. (New York, NY) for 30, 60, 90, and 120 minutes. Controls included untreated tap water and tap water supplemented with 100 ppm NH₄NO₃. PAW properties- pH, EC, NO₃-N, NO₂-N, and H₂O₂- were analyzed immediately after treatment and at 5, 24, 48, 72, and 96-hours post-treatment. PAW was applied to pea microgreens in two experimental sets: (1) within 12-hour post-generation and (2) after 72-hour of storage at ambient temperature. PAW initially exhibited lowered pH in all treatments; but after 96 hours in the first experiment, pH of the 30-minute treatment increased to 7.68±0.01 compared to tap water (7.62±0.01), while in the second experiment, all treatments remained lower. NO3-N increased with processing time and storage duration compared to tap water. Fresh weight of microgreens receiving immediately generated PAW exceeded that of tap water (979.48±26.88 g/m²) except for the 120-minute treatment (864.48±66.08 g/m²). After 72 hours of storage, all PAW treatments improved fresh weight compared to tap water, with 60-min and 90-min treatments exceeding NH₄NO₃ by 4.06% and 6.11%, respectively. Shoot length decreased slightly with 120-min PAW (-1.06%) compared to tap water in the first experiment but increased across all treatments in the second. Storing 120-min PAW for 72 hours raised total N pea shoot content (7.66±0.079%) compared to tap water (7.48±0.136%), while Ca levels increased in all PAW treatments relative to tap water and NH₄NO₃. These findings suggest that PAW processing and storage time influence reactive species and nitrogen levels, impacting microgreen yield and quality. Overall, these results highlight the potential dual function of PAW in the sustainable production of microgreens: i) as sanitizer and/or as eustressor immediately post-generation and ii) as a synthetic nitrogen fertilizer replacement after storage.
Speakers
SS

Sanchita Saha

The Pennsylvania State University
Co-authors
FD

Francesco Di Gioia

Pennsylvania State University
Friday August 1, 2025 12:30pm - 12:45pm CDT
Foster 2
  Oral presentation, Produce Quality Safety and Health Properties
  • Subject Produce Quality Safety and Health Properties
  • Poster # csv
  • Funding Source The research was funded by the Pennsylvania Department of Agriculture Specialty Crop Block Grants No. C940001529 “Developing Plasma Agriculture Solutions to Improve Vegetable Yield and Quality” and was supported by Plasma Licensing Authority Inc. that provided us with the plasma generator, and by the Strategic Networks and Initiatives Program (SNIP) “Developing the Penn State Interdisciplinary Initiative on Plasma Agriculture”, funded by the Penn State College of Agricultural Sciences. FD's contribution was funded by the USDA National Institute of Food and Agriculture and Hatch Appropriations under Project #PEN05002, Accession #7007517.

12:45pm CDT

QUAL - Postbiotic Biopolymers as Natural Antimicrobial Emulsifiers for Post-Harvest Preservation and Food Safety
Friday August 1, 2025 12:45pm - 1:00pm CDT
Ensuring food safety and extending shelf life remain key challenges in post-harvest management of fresh produce. This study investigates the potential of postbiotic biopolymers, specifically Lactobacillus-derived exopolysaccharide (EPS), as a natural and sustainable emulsifier for stabilizing the bioactive antimicrobial volatiles limonene, and eugenol in oil-in-water nanoemulsions. The developed EPS-based nanoemulsion exhibited strong antimicrobial activity against Escherichia coli, Listeria monocytogenes, and Salmonella Poona, achieving >3.0 log reductions on fresh lettuce through immersion and spray applications, while preserving sensory attributes such as texture, color, and taste. Additionally, the nanoemulsion demonstrated a bacteriostatic effect, achieving up to a 3.17 log CFU/cm² biofilm reduction on stainless steel and plastic surfaces (p < 0.05), and a 3-log reduction of Salmonella and E. coli on tomato and blueberry fruit surfaces, along with a 4-log reduction of L. monocytogenes on the soft cheese queso fresco. Stability assessments confirmed that the emulsions remained highly effective across diverse environmental conditions, including temperatures of −20 to 70°C, pH 2–9, and salinity levels from 1%–30%, making them well-suited for real-world agricultural and food processing applications. The synergistic action of EPS and bioactive volatiles not only prolonged shelf life and reduced microbial colonization but also provided a non-toxic, biodegradable alternative to synthetic preservatives, addressing sustainability concerns in food safety and post-harvest preservation. These findings highlight EPS-based nanoemulsions as a promising alternative for enhancing food safety, reducing microbial risks, and supporting sustainable post-harvest practices across fresh produce, dairy, and food-contact surfaces. This work was supported by USDA-NIFA-2024-51181-43464 through the Vegetable and Fruit Improvement Center of Texas A
Speakers
SB

SANGEETA BALYAN

Graduate Student, Texas A & M
Co-authors
DK

Deepak Kumar Jha

Texas A&M AgriLife
VD

Vikas Dadwal

Texas A&M University
I am experienced in phytochemical extraction and LC-MS metabolomics of pre and post-harvested fruits and vegetables. My research focuses on the development of plant-derived bioactive formulations and the assessment of their biological activity in vitro and in vivo. I am currently... Read More →
Friday August 1, 2025 12:45pm - 1:00pm CDT
Foster 2
  Oral presentation, Produce Quality Safety and Health Properties
  • Subject Produce Quality Safety and Health Properties
  • Poster # csv
  • Funding Source This work was supported by USDA-NIFA-2024-51181-43464 through the Vegetable and Fruit Improvement Center of Texas A&M University and partially funded by the Texas A&M Institute for Advancing Health through Agriculture

1:00pm CDT

QUAL - Assessing the Impact of Biological Soil Amendments of Animal Origin on Radish Microbial Safety, Soil Properties, and Quality
Friday August 1, 2025 1:00pm - 1:15pm CDT
The need to shift toward sustainable food production systems has forced farmers to explore alternative practices, such as the use of biological soil amendments of animal origin (BSAAOs), to fulfill consumer-driven requirements. However, concerns due to food safety regulations restrict the use of BSAAOs on crops consumed raw. The present study evaluated the effects of untreated BSAAOs, raw poultry litter (PL), cow manure (CM), and no BSAAO on soil fertility, microbial contamination indicators, and marketable yields in radish (Raphanus sativus) production over two growing seasons at the LSU AgCenter Burden Botanical Gardens. In the agronomic assessment, radish marketable yields were significantly enhanced by PL application, with an average yield of 8.13 lb. per 3.66 m² plot compared to 4.24 lb. in CM-treated plots and 4.62 lb. in no BSAAO amended plots. Moreover, the method of manure application further influenced radish yields, with tilled PL (PLT) plots yielding 9.45 lb. per plot versus 6.81 lb. in non-till (PLNT) plots. Poultry litter application sustained higher levels of nitrogen through the production cycle as well, during Year 1 only, in comparison to CM and no BSAAO application. The evaluation of the safety of raw manure application focused on indicator organisms. In Year 1, soil samples from PL-treated plots exhibited significantly higher Escherichia coli (E. coli) levels (1.81 and 1.75 log CFU/g, respectively, for tilled and not tilled plots) relative to no BSAAO and CM-amended plots (≤1 to 1.01 log CFU/g), while harvested radish samples from PL-amended plots registered 0.8 log CFU/radish compared to non-detectable levels (≤0.75 log CFU/radish) for CM and no BSAAO-amended plots. In Year 2, soil samples from cow manure tilled (CMT) plots had much higher E. coli counts at first (3.23 log CFU/g in Week 1), but these dropped to 1.17 log CFU/g by Week 3, while other treatments kept their counts steady around or below 1 log CFU/g. Results obtained in the present study demonstrate that raw poultry litter substantially improved radish yields by supplying richer nutrient inputs; it also elevated soil and produces microbial contamination indicator levels relative to cow manure and conventional chemical fertilizer. Although radish surface contamination by E. coli remained low, the observed trends highlight the necessity for optimized manure application practices, particularly regarding incorporation methods and waiting periods, to mitigate potential food safety risks and prevent foodborne illness outbreaks.
Speakers
DL

Daniel Leiva

Research Assistant, Louisiana State University
Co-authors
AA

Achyut Adhikari

Louisiana State University AgCenter
NA
KF

Kathryn Fontenot

LSU AgCenter
LF

Lisa Fultz

United Stated Department of Agriculture
NA
Friday August 1, 2025 1:00pm - 1:15pm CDT
Foster 2
  Oral presentation, Produce Quality Safety and Health Properties

1:44pm CDT

POM 4 - Oral Session
Friday August 1, 2025 1:44pm - 1:45pm CDT
Presiding/Moderator
avatar for Brent Black

Brent Black

Utah State University
NA
Friday August 1, 2025 1:44pm - 1:45pm CDT
Foster 2

1:45pm CDT

POM 4 - Adjusting the carbohydrate balance sub-models for use in Washington State
Friday August 1, 2025 1:45pm - 2:00pm CDT
Precision crop load management is important for increasing yield, fruit size, and quality of apple production, particularly for the ‘Honeycrisp’ variety, which is highly susceptible to biennial bearing and difficult to thin. The MaluSim model, developed at Cornell University, can be used to guide crop load management by modeling carbohydrate balance to optimize fruitlet thinning. However, this model was developed using tree performance in New York and may not account for higher temperatures in Washington State, which may affect canopy growth and development. The objective of this study was to assess early season canopy growth and evaluate carbon partitioning of Honeycrisp apples grown in Washington state climatic conditions. The first experiment compared the canopy growth of Honeycrisp topworked in 2016 onto a ‘Granny Smith’ planting with M.9T337 as a rootstock. The topworked trees were trained to single, double, or triple-axis trees. The second experiment assessed carbon partitioning of Honeycrisp trees conducted under single, double, and triple leader(s) training systems. Training systems significantly impacted shoot length and the number of shoots. Single-axis trees had significantly longer shoot lengths and higher shoot numbers than the double and triple-axis training systems. Canopy imaging was also used to assess canopy infill and light interception. The second experiment weighed the biomass partitioning of the various tree parts (spurs, 1-year growth, leaves, branches, roots, rootstock, inter-stem, and fruits). Below-ground portions of all training systems accounted for more biomass than above-ground portions. There was smaller wood and a greater proportion of spur buds in the double and triple-axis trees compared to single-axis trees. The differences in carbon partitioning and canopy development among different training systems compared to the types of trees used when developing the MaluSim model may affect how the MaluSim model performs.
Speakers
HA

Harriet Ampofo

Washington State University-TFREC
Co-authors
LK

Lee Kalcsits

Washington State University-TFREC
NA
Friday August 1, 2025 1:45pm - 2:00pm CDT
Foster 2
  Oral presentation, Pomology 4
  • Subject Pomology
  • Poster # csv
  • Funding Source USDA-SCBGP

2:00pm CDT

POM 4 - Improving Apple Crop Load Management Using Digital Tools: Matching Data Collection With Accurate Treatment Application
Friday August 1, 2025 2:00pm - 2:15pm CDT
Multiple digital imaging companies can gather apple orchard data to map flower bud load, flower cluster number and fruitlet number at the tree level. However quickly matching photographic survey data to maps which can accurately guide management decisions remains challenging. Over the past year, Cornell University has partnered with several companies which can collect and apply survey data to guide precision pruning, precision blossom thinning, and precision fruitlet thinning in high density apple orchards of Western New York. Photographs and surveys from companies which collect single tree information provided the highest resolution information to guide precision sprayers. Precision sprayers were able to successfully apply treatments to the tree level, but the survey data first needed to be transformed into task maps which defined unique tree positions using the same GPS system used to collect the data and then control the sprayer to avoid an offset. Improving orchard management using digital tools may help improve crop load management but the success of this effort depends on the treatment resolution (section of row vs individual tree) as well as when crop load was modified, such as pruning, blossom, and or fruitlet timing.
Speakers
avatar for Brian Lawrence

Brian Lawrence

Cornell University
Co-authors
TR

Terence Robinson

Cornell University
NA
YJ

Yu Jiang

Cornell University
Friday August 1, 2025 2:00pm - 2:15pm CDT
Foster 2
  Oral presentation, Pomology 4

2:15pm CDT

POM 4 - High relative growth rates during early fruit development in apple are facilitated by processes that promote cell production
Friday August 1, 2025 2:15pm - 2:30pm CDT
Fruit growth across various stages of development determines the extent of final fruit size, and is therefore of high economic value. The two main processes that mediate growth, cell production and cell expansion, contribute differentially to growth across various stages of fruit development. In apple, growth during early fruit development is largely facilitated by cell production. Mid and late stages of fruit development display growth mediated mostly by cell expansion. Mechanisms that regulate these processes and consequently the growth rates of fruit are not well understood. The objective of this study was to determine the molecular processes associated with higher relative growth rates (RGR) in the apple fruit during different stages of development. To address this objective, we exploited the inherent natural variation in RGR within a population of ‘Empire’ fruit. These evaluations were performed during early and mid-fruit development stages. At each stage, change in fruit size (volume) was determined across a 4 d interval to obtain the RGR. The population of fruit was grouped into two classes based on their RGR values: High and Low RGR. RNA-sequencing analyses were performed to compare the transcriptomes of the two classes of fruit. During early fruit development, the High RGR class fruit displayed 1.46-fold greater growth rates than the Low RGR fruit. Transcriptome analysis indicated that genes associated with cell division processes such as HISTONES, CYCLINS and CYCLIN DEPENDENT KINASES were more abundant in fruit with higher RGR. During mid-fruit development, fruit displayed relatively lower RGR. Yet the High and Low RGR fruit classes differed by 1.76-fold. However, only a limited set of genes, mostly associated with oxidation-reduction processes, were differentially expressed across these two groups. Overall, these data suggest that processes that regulate cell division control the growth rates of apple fruit during early fruit development.
Speakers
AM

Anish Malladi

University of Georgia
Co-authors
BR

Bayleigh Roussel

University of Georgia
Friday August 1, 2025 2:15pm - 2:30pm CDT
Foster 2
  Oral presentation, Pomology 4

2:30pm CDT

POM 4 - Rethinking Pollination and Crop Load Management: Multi-Year Evaluation of Insect-Exclusion Netting in Apple Orchards
Friday August 1, 2025 2:30pm - 2:45pm CDT
Thinning remains a critical yet challenging practice in apple production, especially for organic systems where chemical tools are limited. Since 2017, our research has evaluated the use of insect-exclusion netting as a sustainable, non-chemical strategy to simultaneously manage crop load and reduce pest pressure. What began as small-scale trials has expanded to commercial-scale implementation in Michigan, supported by USDA funding. Over the course of eight years, we evaluated netting on a diverse suite of cultivars including 'Gala', 'Fuji', 'SweeTango’, Liberty’ and 'Honeycrisp' in multiple locations. Canopies were enclosed at varying bloom stages, and results consistently demonstrated that netting significantly reduced fruit set in proportion to the amount of open bloom at the time of application. Cultivar-specific responses were observed: Gala’ and 'Fuji' achieved optimal crop loads when netted between 25% and 50% bloom, while ‘SweeTango’ and 'Honeycrisp' often required higher percentage of open bloom. In several trials, netted trees yielded commercial fruit numbers without requiring hand or chemical thinning. Fruit size and quality remained similar to conventional treatments, while seed content was reduced without negatively impacting fruit development. In many cases, trees netted at the ‘pink’ phenology stage produced adequate fruit set of low seed-content fruit despite the exclusion of pollinators. Importantly, molecular genotyping using 16 SNP markers revealed that seed parentage in netted canopies overlapped with known pollinizer genotypes. This ruled out self-pollination and suggested that wind, rather than insects, facilitated cross-pollination under netted conditions. Additionally, fruit retention was linked to seed content at the population level, not the individual fruit level, further supporting the viability of non-insect pollination under netting. In 2024, we implemented nets in commercial orchards in Michigan to validate scalability. Netting treatments at 30% and 60% King Bloom produced yields and fruit quality metrics comparable to conventional thinning. Pest monitoring confirmed male codling moth exclusion and reduced San Jose scale captures, though woolly apple aphid and flower thrips populations increased under nets given the exclusion of natural predators Collectively, our results indicate that exclusion netting is a promising, multifunctional tool for organic and sustainable apple production. Beyond thinning and pest control, netting systems enable effective crop set through wind-mediated pollination, challenging traditional assumptions of insect dependency. Adoption of this system may require revised orchard design to optimize pollen flow, but offers significant environmental benefits, including reduced chemical inputs, support for pollinator conservation, and improved climate resilience in Midwestern apple orchards.
Speakers
ME

Mokhles Elsysy

Michigan State University
Co-authors
TE

Todd Einhorn

Michigan State University
NA
Friday August 1, 2025 2:30pm - 2:45pm CDT
Foster 2
  Oral presentation, Pomology 4
  • Subject Pomology
  • Poster # csv
  • Funding Source SARE

2:45pm CDT

POM 4 - Fruit Doubling in Montmorency Tart Cherry (Prunus cerasus)
Friday August 1, 2025 2:45pm - 3:00pm CDT
Heat or drought stress during critical stages of bud differentiation can result in the formation of double pistils in many stone fruits. If these flowers are pollinated, the resulting malformed “double” or “twin” fruit are not marketable. The frequency of double fruit in Utah tart cherry orchards has increased in recent years. To determine the critical time of flower bud formation for doubling, whole-tree treatments of induced heat stress were imposed for one-week intervals from July to September in 2022 and 2023. The resulting doubling incidence was observed in the following spring and at harvest. There were seasonal differences in the frequency of doubled fruit between years, but the highest incidence of doubling in both years resulted from induced heat stress at 4 to 6 weeks after harvest. Understanding this timing is important in targeting alleviation strategies such as evaporative cooling or spray-on orchard protectants.
Speakers
avatar for Brent Black

Brent Black

Utah State University
NA
Co-authors
JM

Josette Moon

Utah State University
NA
Friday August 1, 2025 2:45pm - 3:00pm CDT
Foster 2
  Oral presentation, Pomology 4
  • Subject Pomology
  • Poster # csv
  • Funding Source Utah Department of Agriculture and Food Specialty Crop Block Grant

3:00pm CDT

POM 4 - Diversity and Abundance of Insect Pollinators in Pawpaw (Asimina triloba), an Emerging Commercial Fruit Crop
Friday August 1, 2025 3:00pm - 3:15pm CDT
Pawpaw (Asimina triloba) is a temperate, deciduous fruit tree native to North America and a member of the custard apple family (Annonaceae). It produces unique, flavorful fruit with a taste resembling mango, banana, and pineapple, making it valuable for various value-added products such as jams, bread, ice cream, and brandy. Pawpaw fruits are also rich in nutrients, vitamins, and antioxidants, contributing to their increasing commercial potential. However, fruit sets in some cultivars may be limited due to the protogynous and self-incompatible nature of pawpaw flowers, which require external pollinators for successful fertilization. Unlike many fruit-bearing species, pawpaw has been thought to be pollinated by beetles and flies rather than bees. However, their abundance and role in pollination remain understudied. This study aims to identify and quantify insect visitors to flowering and non-flowering branches of two pawpaw cultivars, ‘Sunflower’ and ‘Susquehanna’. Ten trees of each cultivar were selected, and a completely randomized design was implemented setting up 20 wire cage traps coated with tangle-trap adhesive (The ORTHO Group, Marysville, OH 43040) on flowering and 20 on non-flowering branches. After an 18-day flowering period, the traps were collected, and captured insects were identified to the level of order and their abundance recorded. In 2024, 20 traps were placed at control sites outside the orchard (roadsides and fields), and nine days of direct observations were conducted to document insect activity on male and female flower stages. Data were analyzed using RStudio v.2023.09.1 (Posit, PBC, Boston, MA) and subjected to an ANOVA, with flowers, cultivars, and years as treatment factors, followed by Least Significant Difference (LSD) means separation. Results from 2023 indicate a significantly higher abundance (p-value< 0.0001***) of Coleopteran insects on both flowering and non-flowering branches of ‘Sunflower’ and ‘Susquehanna’ compared to 2024. In 2023 Coleoptera was significantly higher in the flowering branch (p-value 0.0255*) and had an interaction of cultivar and flowers (p-value 0.04767*) while in 2024 Diptera was substantially higher in the non-flowering branches (p-value 0.04009 *) This study provides valuable insights into the pollination ecology of pawpaw, aiding in the development of strategies to enhance fruit set and improve commercial production as the species transitions into broader commercialization.
Speakers
ST

Subas Thapa Magar

Kentucky State University
A major change happened when the tragedy of Corona hit the world. Hi, I am Subas Thapa Magar, a graduate research assistant under the supervisor Dr. Kirk Pomper. I am currently working on Pawpaw (Asimina Triloba), the native fruit tree of the United States of America, and my research... Read More →
Co-authors
JL

Jeremy Lowe

Kentucky State University
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
SC

Sheri Crabtree

Kentucky State University
Friday August 1, 2025 3:00pm - 3:15pm CDT
Foster 2
  Oral presentation, Pomology 4
 


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