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Tuesday, July 29
 

12:15pm CDT

BnB - Biochar-absorbed Nutrients from Anaerobic Digestion Affect the Growth and Quality of Lactuca sativa ‘Newham’
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Biochar, a carbon-rich material produced from biomass pyrolysis, has potential to absorb and provide mineral nutrients for plant growth. Anaerobic digestion is a process where microbes decompose organic material without oxygen in sealed vessels to produce digestate and biogas. In this study, biochar was utilized to sequester mineral nutrients during anaerobic digestion of vegetable waste and used to deliver nutrients to a growing leafy green vegetable. Fresh vegetable biomass was used as a feedstock in a novel 80 L cartridge anaerobic digester in Spring 2024. Biochar was placed in the digester liquid effluent for a minimum of 30 days to absorb nutrients liberated by the digestion process. Biochar was analyzed for nitrogen, phosphorus, and potassium content to determine plant culture treatment levels. A plant culture greenhouse experiment (lat. 40.51ºN; Normal, IL) was conducted in Fall 2024. Seeds of lettuce (Lactuca sativa ‘Newham’) were grown to transplant size. One week after seedling emergence, a soluble 20N-4.4P-16.6K fertilizer was applied to seedlings every seven days at a rate of 100 mg/L N. Approximately three weeks after emergence, individual plants were transferred to 3.8 L plastic pots filled with soilless media. Fertility treatments were maintained at 70 mg/L N using the following ratios of commercial 20N-4.4P-16.6K Fertilizer:Biochar;100%:0%, 90%:10%, 80%:20%, 70%:30%, and 60%:40% by weight, respectively, as a media-incorporated, preplant application. Pots were arranged in a completely randomized block design. During plant growth, media Electrical Conductivity (EC) and leaf Soil Plant Analysis Development (SPAD) were measured every five days. Media EC decreased (P≤0.001) and leaf SPAD increased (P≤0.001) across all treatments during growth. Lettuce was harvested at 50 days post-seeding when it reached marketable size. At harvest, shoot and root fresh mass were measured, and fresh tissue was dried at 60ºC for dry mass determination. For lettuce, total biomass, shoot FM, root FM, shoot DM, and root DM at harvest all decreased, and then increased quadratically (P≤0.001) as Fertilizer:Biochar ratios changed from 100%:0% to 70%:30%. The highest lettuce per plant total biomass was 176.92 g per plant under 100% commercial fertilizer. Harvest EC increased linearly (P≤0.001) and SPAD decreased, then increasing quadratically (P≤0.001) as Fertilizer:Biochar ratios changed from 100%:0% to 60%:40%. Observed Fertilizer:Biochar response patterns indicate a delayed nutrient release from biochar over time. Additional research on the timing of nutrient release from biochar and possible uses as a commercial fertilizer for vegetable crop production continues to be warranted.
Speakers
DK

David Kopsell

Illinois State University
Co-authors
AG

Abbie Grosage

Illinois State University
NA
CM

Caden Miller

Illinois State University
NA
LY

Liangcheng Yang

Illinois State University
NA
MM

Md. Mahadi Hasan

Illinois State University
NA
TY

Tuba Yasmin Lubna

Illinois State University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Bioenergy and Co-Products
  • Funding Source USDA-NIFA Capacity Building Grants for Non-land Grant Colleges of Agriculture Program (NLGCA)

12:15pm CDT

BnB - Effects of Different Seed Treatments on Germination of Advanced Guayule Lines
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Guayule, Parthenium argentatum Gray, is a perennial shrub native to the Chihuahuan desert of Mexico and Texas. It is known for its latex and rubber production, which are viable alternatives to Hevea rubber. However, guayule seeds have low germination rate, which doesn’t meet commercial requirements. Guayule seeds have a germination rate between 55% and 87%, but this is highly dependent upon priming and soil temperature. Newer guayule lines have been developed, but the germination rate still varies. There is very little literature on successful priming methods in guayule seeds, most of which have been performed on historic lines in the 1980’s and 1990’s. To determine how newer lines are affected by different priming methods, we treated seeds with seven priming treatments and a control, then studied germination rates over 14 days. The treatments included: treatment 1 – control; Treatment 2 – Presoaking in 25% PEG, 10-4 M gibberellic acid, 0.05% KNO3, and 0.1% thiram fungicide adjusted to a pH of 8 with Ca(OH)2 and soaked for 4 days; Treatment 3 – Mildly scarified with fine sandpaper; Treatment 4 – Treatment 2 scarification; Treatment 5 –DI water soaking for 6 hours; Treatment 6 – Soaking with 75 ppm NAA and IAA, 1M KH2PO4 for 24 hours; Treatment 7 – Soaking in a DI water/ 0.1 M KNO3 solution for 12 hours; and lastly treatment 8 – Soaking in DI water for 8 hours, followed by soaking them for 2 hours in a solution of equal parts gibberellic acid (200 ppm) and 0.25% NaOCl. There were significant effects of treatment, line, and interactions of line and treatment on seed germination. Scarification had the overall highest germination percentage, followed by soaking in DI water for 6 hours and the control. The lowest germination was seen in lines soaked in DI water, followed by a bleach and gibberellic acid solution. Line E008 showed the highest germination rate, followed by E001, E009, E002, and E010. The interaction effects were highly variable, but indicated that each line was affected differently by the seed treatments. These results show that treatments to improve germination may need to be tailored to specific lines to optimize germination in guayule.
Speakers
PD

Phiala Drake

Texas Tech University
Co-authors
CS

Catherine Simpson

Texas Tech University
KC

Katrina Cornish

USDA ALARC
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Bioenergy and Co-Products
  • Funding Source U.S. National Science Foundation ​ under Cooperative Agreement No. 2330145

12:15pm CDT

BnB - Greenhouse Evaluation of Three Newly Developed Guayule Lines
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Guayule (Parthenium argentatum Gray) has been produced as an alternative natural rubber crop in the U.S. for over 100 years with varying success. Selecting and breeding for higher yielding rubber and latex has been a priority for establishing guayule as a feasible alternative rubber and latex source, but many environmental conditions affect yields. Previous research has shown regional variability which means regional evaluation of guayule should be conducted for optimal regional line selection. Readily available lines, AZ1 - AZ4, and 11591, among others, were evaluated in the Southern High Plains over 20 years ago. Since then, newer lines have been developed with higher latex yields and quality. Therefore, an experiment was designed to evaluate three of these lines (E002, E003, E004) in greenhouse conditions. Growth parameters, health ratings, canopy temperatures, and bark thickness were measured regularly. Lines E003 and E004 had significantly larger canopy circumferences and plant height than line E002, however, plant health rankings were higher in E003 followed by E002, then E004 lines. Trunk diameter was also greater in E003 and E004 lines, but bark width did not significantly vary among treatments. Bark parenchyma cells are the site of rubber particle production, and therefore as bark thickness increases, rubber may also increase. These results indicate that while the E003 and E004 lines are larger, the rubber production may be higher in E002 in comparison. Many other factors continue to be examined, but these preliminary results further illustrate that line selection is essential, and overall biomass may not be the best factor for selecting high yielding lines.
Speakers
CS

Catherine Simpson

Texas Tech University
NA
Co-authors
KC

Katrina Cornish

USDA ALARC
NA
PD

Phiala Drake

Texas Tech University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Bioenergy and Co-Products
  • Funding Source U.S. National Science Foundation ​ under Cooperative Agreement No. 2330145

12:15pm CDT

CHEX - IPM Extension Program Development in Expanding Commercial Nursery and Green Industries
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Rutgers Cooperative Extension is committed to developing and delivering integrated pest management (IPM) related programming for the commercial nursery, professional landscape industries, and their customer base, home horticulturalists. Here the tactics utilized to develop and deliver IPM programming are highlighted in efforts to assist horticulture professionals navigating an ever-tightening academic personnel climate. The Extension Cycle approach of stakeholder needs assessments, securing funding, educational program development with deliverables, and evaluation has led to significant impacts. In the last year alone, over 300 surveyed stakeholders, representing over 6K acres managed, indicated that they gained knowledge about IPM practices and science-based tools, and nearly 100% plan to adopt these practices, with early-adopters realizing cost savings/profits over $1M. This is important due to size of these industries as the 2019 Census of Horticultural Specialties, indicated $13.8B in sales for floriculture, nursery, and specialty crop goods, while supporting the $153B landscape industry, which provides over 1M jobs nationally (NALP). New Jersey currently ranks fifth in the nation for nursery stock sales ($296M) and third in potted flowering plants ($77.5M), locally supporting around 10K jobs and driving $950M in annual economic impacts (Sullivan & Schilling, 2020). US ornamental industries have unique needs in terms of IPM due to the shear complexity of pests associated with the number of crops grown and diversity of systems. Needs assessments were required to identify key IPM program targets and were compiled through post-program /twilight surveys, state-wide ornamental IPM and native plant needs assessments, and communication with stakeholders. Notable pests emerged and led to the development of educational programs coupled with deliverables including the Rutgers Pest Scouting Guides –Growing Degree-Days, Redheaded Flea Beetle - Predictions, [pictorial] Reference Guide to Common Boxwood Pests and Diseases, Oomycete Material Options, Diagnostic Education Series, and IPM Essentials for Ornamentals. In the last year over 250 education sessions on IPM related topics have been delivered to 15K stakeholders by Rutgers Nursery working group personnel, encompassing over 50 academic, grower/manager, home horticulture, and regulatory groups. Multiple streams of funding were secured to support this work (over $400K in 2024). Dissemination of information has taken many forms; hardcopy monthly newsletters, digitally through listservs and Rutgers Plant and Pest Advisory blog, and bi-weekly in-season lunch hour webinars that address contemporary IPM related topics. Taken together, personnel limited programs are often the norm, thus creatively leveraging the Extension Cycle and homing in on key needs has led to significant impacts.
Speakers
avatar for Timothy Waller

Timothy Waller

Rutgers, The State University of New Jersey
Timothy J Waller, Ph.D. is serving in his fourth year as the Commercial Nursery Agent of Rutgers Cooperative Extension in southern NJ, based out of Cumberland County. Tim has a Ph.D. in Plant Biology with a focus on fungal plant pathology and has been a licensed pesticide applicator... Read More →
Co-authors
WE

William Errickson

Rutgers University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Commercial Horticulture 1

12:15pm CDT

CHMG - Consumer Preferences for Poinsettia Cultivars in South Mississippi in 2024
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
2024 marked Mississippi State University’s South Mississippi Branch Experiment Station’s fourth year to host a Poinsettia Open House. The goal of this annual event was to ascertain the affinity tendencies among retail consumers for poinsettias available in the US market. In addition, five new pre-release varieties not currently offered in any market were included. Results are intended to be shared within the industry. This event took place over a two-day period the first week of December (December 6, 2024 and December 7, 2024). The use of available poinsettias in various floral display arrangements provided participants with ideas for using these versatile plants in holiday inspired settings. Attendees were asked to complete a consumer preference survey in exchange for a complementary poinsettia to take home. The survey design asked for ‘favorites’ within the categories of traditional red, pink, white, novelty, and pre-release varieties. In addition, participants were asked to list their top 3 favorites regardless of the category. Overall, 60 total cultivars across these 5 categories were displayed in small groups of 6-8 plants each. Top votes getters within the 5 categories included: Reds – Christmas Feelings Merlot and Christmas Bells, Pinks – Maren and Jubilee Pink, Whites – Alpina White and Frozen, and Novelties – Superba New Glitter and Premium Ice Crystal. The entries within the pre-release category were identified as numbered selections. The top favorite within this category was a variegated dark pink/cream medium-height plant with large bracts. This new cultivar was also chosen as one of the overall participant favorites joining Superba New Glitter and Alpina White.
Speakers
SL

Scott Langlois

Miss State University
Co-authors
AB

Anthony Bowden

University of Arkansas
BP

Ben Posadas

Mississippi State University
NA
CC

Christine Coker

Mississippi State University
HW

Haley Williams

Mississippi State University
JR

Jenny Ryals

Mississippi State University
JD

Jim DelPrince

Mississippi State University
NA
PR

Patricia R. Knight

Mississippi State University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CHMG - Developing the SEED Garden – Expanding Capacity for Experiential Learning and Demonstrations at the Texas A&M University Leach Teaching Gardens
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
The Leach Teaching Gardens, the first phase of The Gardens at Texas A
Speakers
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Consumer Horticulture and Master Gardeners
  • Funding Source Texas A&M AgriLife

12:15pm CDT

CHMG - KY Farm Launch: A Beginning Farmer Accelerator Program for Kentucky
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
KY Farm Launch is a new beginning farmer training program designed in broad collaboration with organizations working with beginning farmers in Kentucky. This includes University of Kentucky (UK) Cooperative Extension and academic departments, the Kentucky Center for Ag and Rural Development (KCARD), the Kentucky Sheep and Goat Development Organization (KSGDO), and an advisory board of non-profit agencies, beginning farmers, and experienced farmer leaders in Kentucky. The project team implementing the program is a partnership between UK Cooperative Extension, UK's Food Connection, UK Department of Horticulture, UK's Beef Team, KCARD and the KSGDO. This intensive program meets once per week for 9 months in Lexington area, based at UK's Horticulture Research Farm and Organic Farming Unit, UK's Animal Research Unit, and other privately-owned farms supporting the program. Our goal is for program participants to begin farming commercially within one year of completing the program. The program consists of full day of workshops each week focused on production knowledge, hands-on skill building, and farm business development. All participants complete a 'Common Curriculum' of business and market planning, financial record keeping, risk management, and farmland evaluation and assessment (~8 weeks). The bulk of the program is divided into two tracks that participants select based on their primary commercial enterprise: the Sustainable Vegetable Small Fruit Track or the Diversified Livestock Track. The 'Track-Based Programming' (~24 weeks) is applied, hands on training in essential aspects of commercial production of diversified vegetables and small fruits (Veg Small Fruit Track) or sheep, goats, small flock poultry, and small beef cattle herds (Livestock Track). Additionally, each participant works with the team to develop individualized mentoring plans to help fill any gaps in their knowledge and interests. For farmers without access to land, this program is also supporting the development of a beginning farmer incubator program in a partnership between UK and private landowners. This individually-tailored, cohort-based, hands-on approach is designed to accelerate participants progress to successful commercial farming at goal levels developed through a facilitated mentoring process. The program is capped at 30 farms (individuals or farm family units) per cohort cycle. The program will run for at least two consecutive cohort cycles (2025 and 2026). This poster will focus on the curriculum, learning objectives, methods and preliminary outcomes of the Veg Small Fruit track at the mid-way point through our first cohort.
Speakers
KJ

Krista Jacobsen

University of Kentucky
Co-authors
KV

Katie VanValin

University of Kentucky
NA
KD

Kristi Durbin

University of Kentucky
NA
MW

Mark Williams

University of Kentucky
NA
SG

Spencer Guinn

Kentucky Center for Agriculture
NA
VH

Viktor Halmos

University of Kentucky
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Consumer Horticulture and Master Gardeners

12:15pm CDT

CHMG - Rutgers Master Gardener Program Survey: Volunteers, Leaders, Peers Produce Unexpected Results
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Rutgers Master Gardeners (RMG), a 45-year-old program with over 2,000 active volunteers, assist the Agriculture and Natural Resources Department of Rutgers Cooperative Extension in the implementation of its goals. The Extension’s goals include the provision of accurate, research-based, practical information regarding horticulture best practices to extended community of New Jersey. The program evolved organically since its inception and there has been no statewide research that examined the program’s operation and impact. In July of 2024, an extensive survey was sent to 2250 volunteers categorized “active” in the master database. This survey, Volunteer (1), solicited information regarding the volunteers’ motivation for serving, their understanding of the program’s operation, training and funding and tested some future program ideas. A second survey, Volunteer (2), followed. This survey clarified or further explored items from Volunteer (1) and added an impact assessment. A third survey, Rutgers Staff/Faculty, aimed to determine alignment of staff/faculty perception with those of the volunteers as well as to examine the staff/faculty perception of the program’s operation, training and funding. A fourth survey, National, was distributed through the Extension Master Gardener list serve to staff and faculty administering each state’s Master Gardener Program. It examined the alignment of Rutgers program with the national program to obtain a deeper understanding of Rutgers work. All data collected should be considered perception and opinion data. There was both alignment and misalignment were found within and between all surveys. Some unexpected data reveal potential anti-volunteer bias and lack of program leadership clarity. On-going focus group sessions and further surveys and workshops continue to clarify trends. Trends identified, include suggest restructuring of program staffing, enhancing strategic planning and focusing on professional development and support of volunteer coordinators. Data informs the initiation of a strategic planning initiative for the statewide program.
Speakers
avatar for Ruth Carll

Ruth Carll

State Leader, Consumer Horticulture, Rutgers
Ruth Carll serves as the State Leader of Consumer Horticulture and Master Gardener Programs for Rutgers University. She is an innovative leader in the botanical education field. After earning her Master of Botany from Arizona State University, she led the education and volunteer program... Read More →
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CITR 1 - Advances in digital phenotyping and data management using open-source Citrus Genome Database, Breeding Information Management System, and Field Book in citrus breeding programs
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Citrus is one of the most widely grown fruit crops worldwide with a total of $2.98 billion in productivity in the U.S. However, the citrus industry has been significantly affected by Huanglongbing (HLB) which caused as much as 90% production loss in Florida. Efficient phenotyping and data management will aid breeding selection and hasten the development of high yielding citrus varieties with durable resistance to HLB. The Citrus Genome Database (CGD, www.citrusgenomedb.org), an integrated web-based community database for citrus genomic, genetic, and breeding research, offers the Breeding Information and Management System (BIMS) for efficient public or private data management. BIMS is compatible with Field Book, an Android app which allows breeders to collect data on phones or tablets. In addition, the compliance of BIMS and Field Book with the Breeding API (BrAPI) enables seamless data transfer between them. This transition to electronic data collection and data management using BIMS has boosted the efficiency of the University of Florida citrus breeding program. Under the 2022-funded SCRI-project ‘Advanced National Database Resources for Specialty Crop Research and Improvement’, we aim to collaborate with citrus breeding programs across the U.S., offering in-person or webinar training on BIMS and Field Book, while sharing updates on these tools with citrus breeders.
Speakers
RT

Ronald Tapia

University of Florida/IFAS Citrus Research and Education Center
Co-authors
BE

Bryan Ellerbrock

Clemson University
NA
DM

Dorrie Main

Washington State University
FG

Frederick G. Gmitter Jr.

University of Florida/IFAS Citrus Research and Education Center
NA
JH

Jodi Humann

Washington State University
SJ

Sook Jung

Washington State University
NA
TL

Taein Lee

Washington State University
NA
TR

Trevor Rife

Clemson University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CITR 1 - Impacts of Organic Amendments on HLB-affected Citrus Tree Growth and Root Development
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Nutrient availability is an essential aspect of plant growth and development. Sandy soils have low nutrient-holding capacities. Huanglongbing (HLB) trees have lower fine root density and thus decreased capacity to uptake nutrients. Organic amendments have been known to increase nutrient-holding capacities in sandy soils and provide additional nutrients. The objectives of this study were to 1) determine the influences of organic amendments on citrus production on sandy soil, and 2) assess the potential of organic amendments to alleviate the negative impacts of HLB. The study was conducted over six months in a greenhouse setting at the Citrus Research and Education Center in Lake Alfred Florida using Valenica sweet orange on Swingle citrumelo rootstock grown on Candler fine sand. Treatments of compost (5% and 10% by volume) and peat moss (5 and 10% by volume) and a control composed of Candler fine sand without amendment were applied to a total of 50 trees, 25 of which were HLB-affected and the other 25 were healthy trees. The following variables were measured: tree diameter, tree height, leaf chlorophyll content, soil nutrient content, tissue nutrient content, soil organic matter content, and biomass and nutrient accumulation. Organic amendments improved soil organic matter content by 20 to 40%. Organic amendments also increased plant and root growth parameters and demonstrate an ability to alleviate the negative impacts of HLB. Our research will eventually be followed up by field studies to validate the greenhouse observations.
Speakers
TT

Therese Thompson

University of Florida
Co-authors
DK

Davie Kadyampakeni

University of Florida
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source USDA
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

12:15pm CDT

CITR 1 - Incorporation of biochar produced from citrus branches improves soil water holding capacity and nutrient cycling
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Senescence of branches or twig dieback due to tree age or disease in mature citrus orchards (>8 years old) is common. Moreover, strategic pruning and hedging are standard practices to sustain tree health and yield. As a result of these practices, a large amount of biomass without economic value is either left in the field, mulched into the soil, or disposed with minimum control on nutrient recycling. This study aimed to use this biomass as feedstock for biochar production. We pyrolyzed this material to produce biochar with modest amounts of macro and micronutrient content. This citrus-based biochar was applied a fixed rate to return the equivalent of 10 kg N ha-1, and contrasted with the other commercially available biochars. Preliminary data indicates that all biochars were capable of increasing soil moisture retention compared with the control. Fertility analysis of each biochar indicates diverse availability of macro and micronutrients that can complement current fertilization programs. Moreover, biochar incorporation as soil health management strategy may increase the recalcitrant pool of carbon in the soil, with its accompanied benefits. Future research aims to evaluate soil microbial respiration affected by biochar application.
Speakers
HR

Hemanth Reddy

Texas A
NA
Co-authors
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Citrus Crops 1
  • Poster # csv

12:15pm CDT

CITR 1 - Soil Microbiome Response to Cover Cropping in HLB-Affected Citrus Groves: Insights from a Three-Year Field Study in Southeast Florida
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Huanglongbing (HLB), or citrus greening, has severely impacted citrus production in Florida, with no known cure currently available. Growers have increasingly adopted soil-focused management strategies, including cover cropping, to improve soil health and support tree resilience. In the Indian River District, where sandy soils with low fertility and organic matter prevail, the potential for cover crops to influence the soil microbiome remains underexplored. To investigate this, a three-year field trial was established in collaboration with a commercial grower and the University of Florida, IFAS, Indian River Research and Education Center, in Ft. Pierce. The trial included ‘Star Ruby’ grapefruit trees grafted on ‘US-942’, ‘Bearss’ lemon trees grafted on ‘Sour Orange’, and ‘OLL’ sweet orange trees grafted on ‘US-942’ rootstock. A randomized complete block design was used to compare conventional management (no cover crops) to a cover crop system, with species planted biannually and terminated at the end of each season. Soil microbiome composition and diversity were assessed seasonally through high-throughput sequencing, alongside measurements of soil nutrients, organic matter, moisture, and temperature. Early results suggest that the use of cover crops may influence soil microbial communities, with some differences observed between treatments over the three-year period. These include modest shifts in microbial diversity and structure, although the functional significance of these changes remains unclear. These findings suggest that cover crops may influence the soil microbiome in HLB-affected citrus groves, potentially contributing to improved soil conditions and offering a possible strategy to support long-term sustainability in southeast Florida citrus production systems.
Speakers
avatar for Lorenzo Rossi

Lorenzo Rossi

Assistant Professor, Texas A&M University
Dr. Rossi’s research program focuses on understanding the responses of horticultural crops to environmental stresses, with the goal of developing environmentally sound and effective management strategies. He is a horticulturist with expertise in plant stress physiology, plant biology... Read More →
Co-authors
JF

John-Paul Fox

University of Florida
LH

Lukas Hallman

University of Florida
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Citrus Crops 1
  • Subject Citrus Crops
  • Funding Source USDA NIFA MultiState project #S1070

12:15pm CDT

CITR 1 - Understanding Fruit Retention in Sweet Orange (Citrus sinensis L. Osbeck) Under HLB-Induced Premature Drop Conditions
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Fruit retention is a critical factor for citrus growers, as fruit that remains on the tree to be harvested is the fruit that gets paid for. The challenge has been exacerbated by Huanglongbing (HLB), a disease that significantly increases premature fruit drop, making it essential to identify the key factors influencing fruit retention. In this study, the relationship between fruit size, leaf count, seed viability, and fruit detachment force (FDF) was investigated to better understand what contributes to fruit retention on the tree. A total of 30 fruit per tree in four trees were tagged,fruit size and leaf count on the fruiting branch were recorded and monitored over five weeks., In the second week, Ethephon (150 ppm) was sprayed to induce faster fruit drop. Weekly measurements were taken to track fruit drop. At the end of the study period, the remaining fruit were removed along with their stems to measure FDF, viable seed count, and aborted seed count. The findings indicate a positive correlation between the fruit’s FDF and leaf count, suggesting that fruit with more supporting foliage are more likely to remain attached. Additionally, a negative correlation between the number of aborted seeds and FDF was observed, implying that fruit with more aborted seeds are also more susceptible to detachment. These findings were strengthened by comparing the average values of leaves and aborted seeds between the fruit that had to be manually removed and the fruit that dropped prematurely. The prematurely dropped fruit had a significantly lower number of leaves (9 vs. 16.4 average) and a higher aborted seed number (1.9 vs. 0.9 average). These insights should motivate growers to find therapies that promote leaf growth for a more profitable orchard. The study also points to a need for further investigation into the causes of seed abortion to get a clearer picture of the basic mechanism causing HLB-induced fruit drop.
Speakers
JH

Johann Hiller

University of Florida
Co-authors
TL

Taylor Livingston

University of Florida
NA
TV

Tripti Vashisth

University of Florida
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Citrus Crops 1

12:15pm CDT

FLOR - Comparing rooting hormones for semi hardwood cuttings propagation of Puakenikeni (Fagraea berteriana)
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Puakenikeni (Fagraea berteroana) is a popular flower in lei making in Hawaii, especially during graduation season and mothers day, which coincide with the flowering period. Most of the propagation is done via air layers, to promote early flowering. Propagation via rooted cuttings has been reported in the literature, however, there are is no information in regards to best practice, and it is a common question asked to the the University of Hawaii at Manoa Cooperative Extension Services. This trial used different powder rooting hormones to compare their effectiveness in propagating semi hardwood cuttings of puakenikeni. Cuttings were dipped in the rooting hormone, and the lower 2 inches of each cutting was inserted in a mix of 50% perlite and 50% vermiculite and placed in a mist bench for 2 months. Three treatments were compared (no rooting hormone, and 0.1%, 0.3% and 0.8% IBA). A total of 80 cutings were used, or 20 cuttings per treatment. After two months the control treatment with no rooting hormone had only 10% rooting. The 0.1% IBA and 0.3% IBA had 30% and 10% rooted cuttings, respectively. The 0.8% IBA treatment had 85% rooting success. Results from this trial indicate that a rooting hormone with 0.8% IBA should be used when rooting semi hardwood cuttings of puakenkeni (Fagraea berteroana). Future trials may be performed to compare other concentrations and types of rooting hormones (powder, liquid and gel), in order to achieve higher rooting percentage rates.
Speakers
AR

Alberto Ricordi

University of Hawaii at Manoa
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Floriculture
  • Subject Propagation
  • Poster # csv
  • Funding Source UHM Cooperative Extension and Lei Poinaole Project

12:15pm CDT

FLOR - Effects of Indole-3-butyric acid (IBA) on Agastache ‘Coronado Red’ Stem Cuttings
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
The growing demand for sustainable landscapes and eco-friendly practices has significantly influenced consumer interest in native plant species. Agastache spp. is a xeric species naturally acclimated to the Rocky Mountain region and is valued for its ornamental flowering and exceptional landscape resilience, particularly in arid environments. However, one challenge is the limited availability of saleable plants due to challenges during asexual propagation, in particular, successful adventitious rooting of cuttings. Therefore, a study was conducted to evaluate the impact of different indole-3-butyric acid (IBA) rooting hormone concentrations on root initiation and development of Agastache ‘Coronado Red’. A total of 72 uniform stem cuttings were collected and 18 stems were treated with one of four experimental treatments that included, control (water), 1000, 3000, and 8000 ppm IBA concentrations. Cuttings were stuck in 72-cell trays and placed on a propagation misting bench, using a randomized complete block design. Destructive data collection occurred on a bi-weekly basis, with three collections. The experiment was repeated over the course of two production seasons. Two weeks after sticking, while callus initiation was present on cuttings for all treatments, an increase in both the number of primary roots and root length was observed as the concentration of IBA increased. For example, the number of primary roots was greatest for cuttings provided the IBA concentration of 8000 ppm (16) compared to the control (0.2) and IBA concentrations of 1000 (3.4) and 3000 ppm (6.3). Similarly, length of the longest root was 1891%, 144%, and 62% greater under the IBA concentration of 8000 ppm compared to the control and IBA concentrations of 1000 and 3000 ppm, respectively. Stem length and the number of nodes were similar for all treatments. These results indicate that higher IBA concentrations promote root initiation and growth as early as two weeks after sticking for Agastache ‘Coronado Red’. While results from the present study indicate that an IBA concentration of 8000 ppm is optimal for early establishment of Agastache ‘Coronado Red’ cuttings, future research is required to determine propagation strategies for other cultivars.
Speakers
CT

Cienna Tonini

Colorado State University
NA
Co-authors
CT

Chad T. Miller

Colorado State University
NA
JC

Joshua Craver

Colorado State University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Floriculture
  • Funding Source Colorado Horticulture Research and Education Foundation

12:15pm CDT

FLOR - Evaluation of nitrogen, phosphorus and potassium deficiency in ornamental crop species using a digital phenotyping system
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Plant growth and development depends on essential macronutrients such as nitrogen (N), phosphorus (P), and potassium (K), and deficiencies in these nutrients result in significant physiological and morphological changes. Leaf biochemical and biophysical properties influence light absorption and reflectance across various wavelengths, providing insights into canopy health. Advancements in high-throughput (HT) digital phenotyping technologies, including high-resolution scanning and multispectral imaging, have improved plant health assessment and monitoring. The TraitFinder, a digital phenotyping system developed by Phenospex, is equipped with two PlantEye-600 multispectral 3D laser scanners that generate three-dimensional plant models while capturing multispectral data. The system directs light in green (G), blue (B), red (R), and near-infrared (NIR) wavelengths onto the plant canopy and captures the reflected signals, which are then used to compute vegetation indices for plant health evaluation. This study utilized the TraitFinder system to determine reference values for vegetation indices associated with healthy plants and those deficient in N, P, and K. Four ornamental species—coleus (Solenostemon scutellarioides), marigold (Tagetes patula), petunia (Petunia × hybrida), and celosia (Celosia plumosa)—were evaluated over time. The experiment followed a randomized complete block design with eight replications and four nutrient treatments: a complete Hoagland’s solution and three modified versions, each lacking one macronutrient (N, P, or K). Morphological traits, such as biomass, showed reduced plant growth under nutrient-deficient conditions. Spectral data revealed common trends in nutrient-deficient plants, including decreased Green Leaf Index (GLI) and Normalized Difference Vegetation Index (NDVI) and increased Normalized Pigment Chlorophyll Ratio Index (NPCI) and Plant Senescence Reflectance Index (PSRI) compared to controls. In healthy plants, GLI ranged from 0.2 to 0.35, NDVI from 0.4 to 0.75, NPCI from 0.10 to 0.45, and PSRI from 0.07 to 0.25. However, species-specific responses to nutrient deficiencies were also observed. This study highlights the distinct morphological and physiological responses of ornamental species to macronutrient deficiencies and demonstrates the effectiveness of digital phenotyping using the TraitFinder system for tracking plant health over time. The findings emphasize the potential of HT digital phenotyping which could enhance ornamental crop management.
Speakers
JM

JANHAVI MAURYA

The Ohio state University
Co-authors
JQ

Juan Quijia Pillajo

The Ohio State University
LC

Laura Chapin

The Ohio State University
MJ

Michelle Jones

The Ohio State University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Floriculture
  • Subject Floriculture
  • Funding Source D.C. Kiplinger Endowment

12:15pm CDT

FLOR - Fertilizer Rate and Phosphorus Dose Influences Growth Promotion and Phosphorus Absorption in Lalrise Vita-Treated Marigolds and Pansies
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
High fertilizer costs and environmental impacts are growing concerns in modern agriculture, and the floriculture industry is increasingly adopting the use of biostimulants to enhance plant growth, stress tolerance, and nutrient use efficiency. Despite their potential, the effectiveness and specificity of many biostimulants remain poorly understood. This study evaluated the growth-promoting effects of Lalrise Vita, a commercial biostimulant containing the phosphate solubilizing bacteria Bacillus velezensis. Lalrise Vita's impact on plant growth and health was evaluated using Tagetes patula ‘Durango Yellow’ (French marigolds) and Viola × wittrockiana ‘Clear Yellow’ (pansies). The first experiment evaluated how fertilizer at increasing concentrations affected the plant responses to the biostimulant. A water-soluble fertilizer delivered 50, 150, 300, or 600 mg·L–1 nitrogen (N) from 15N–2.2P–12.5K–2.9Ca–1.2Mg applied once weekly with 100 mL of solution per pot. Lalrise Vita was applied once at transplant at 0.25 g per plant as a substrate drench of 100 mL of solution per pot, with untreated plants as controls for both experiments. The second experiment focused on phosphorus (P) fertilization, with plants receiving increasing P concentrations of 2.5, 5, 10, or 20 mg·L–1 from KH₂PO₄ once a week along with the fertilizer. Plants were fertilized weekly with 100 mL of solution delivering 200 mg·L–1 N from 15N–0P–12.5K–2.9Ca–1.2Mg. Plants were scanned using the Trait Finder (Phenospex), and digital biomass, normalized pigment chlorophyll ratio index, plant senescence reflectance index, and HUE were used to identify differences in plant growth and health. In marigolds, Lalrise Vita improved digital biomass and plant health across all fertilizer rates compared to untreated plants. The greatest differences were observed at lower fertilizer concentrations (50 and 150 mg·L–1 N). Significant improvements were observed in pansies at 50 mg·L–1 N, including increased biomass and improved health indicators. Marigold plants treated with Lalrise Vita at 5 and 10 mg·L–1 P had greater growth, although spectral variables indicated greener foliage at higher P rates in untreated plants. There were no significant differences in pansies between treated and untreated plants, suggesting that Lalrise Vita-treated plants receiving 2.5 mg·L–1 P performed comparably to those receiving 20 mg·L–1 P without Lalrise Vita. Excessive fertilizer (300 and 600 mg·L–1 N) reduced Lalrise Vita’s benefits, as phosphate-solubilizing bacteria are less effective when P is not limiting. However, Lalrise Vita can be used to grow quality crops with reduced fertilizer applications.
Speakers
AR

Ashly Ramos

The Ohio State University
Co-authors
AE

Alexandra Espinoza

The Ohio State University
GO

Garrett Owen

The Ohio State University
JQ

Juan Quijia

The Ohio State University
NA
MJ

Michelle Jones

The Ohio State University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Floriculture
  • Poster # csv
  • Funding Source OSU DC Kiplinger Endowment

12:15pm CDT

FLOR - Growth and development of Uniconazole treated Easter lily bulbs
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Easter lilies (Lilium longiflorum) cultivated in the greenhouse industry are often treated with plant growth regulators (PGRs) to control their height by reducing stem length. A greenhouse study was conducted to examine the effects of uniconazole “Sumagic” on the growth of a new cultivar of Easter lilies ‘White Spring’. Treatments consisted of five bulb sizes based on circumference (12/14, 14/16, 16/18, 18/20, 20/22 cm) and uniconazole rates of 0, 2.5, and 5.0 mg•liter-1 with eight replications. Data collection consisted of stem height measured weekly, days until anthesis, number of flowers, and phytotoxicity rating. The results of the study showed a relationship between the uniconazole treatments and plant height, control plants had a greater average height (57.8 cm) than the bulbs soaked in 2.5 and 5.0 mg•liter-1 uniconazole, (33.5 cm) and (23.8 cm), respectively. Plant heights were also influenced by bulb size as they increased in height with increase in bulb circumference. The average number of days until anthesis increased with smaller bulb circumference, as anthesis was delayed. Days until anthesis were also affected by the uniconazole treatments, the bulbs treated with 2.5 and 5.0 mg•liter-1 solutions took approximately 6 and 8 days longer, respectively. Phototoxicity effects displayed as chlorosis on leaf tips and margins occurred in both the 2.5 and 5.0 mg•liter-1 solutions with symptoms more prominent in larger bulb sizes.
Speakers
MM

Michael Maurer

Stephen F Austin State University
Co-authors
LK

Luke Keene

Stephen F. Austin State University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Floriculture

12:15pm CDT

FLOR - Influence of peat-based substrate pH and plant species on rhizosphere microbial communities
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Soil pH shapes rhizosphere microbial structure and diversity, influencing nutrient cycling, plant growth, and ecosystem health. However, the effects of pH on the microbiome in greenhouse-grown ornamental plants in peat-based soilless substrates are less well understood. This study examined the impact of substrate pH and plant species on rhizosphere bacterial communities to see how the interaction of these factors influenced microbial diversity. A two-factor experiment with substrate pH (4.5, 5.5, 6.2, and 7) and plant species (geranium, marigold, petunia, and tomato) was conducted in a greenhouse with six replicates per treatment (n=6) in a random complete block design. Substrate-only controls were included at each pH level to evaluate plant species influence on the bacterial community. The peat-based substrate pH was adjusted with dolomitic limestone. Plants were fertilized at each irrigation with 100 µg·g-1 N from 15-5-15-Ca-Mg fertilizer. After eight weeks of growth, the ornamental plants were fully flowering, and the tomato plants were beginning to bud. At that time, plant morphology and vegetative indices were evaluated by 3D image analysis, vegetive tissue and substrate leachate were evaluated for nutrient content, and rhizosphere samples were collected to evaluate bacterial composition and diversity by 16S amplicon sequencing. Plant species differentially modified the substrate pH from the starting levels. Geranium and marigold acidified the substrate, tomato tended to increase substrate pH, and petunia maintained pH close to the initial values. Shannon diversity indices indicated that bacterial diversity varied across the pH and plant species treatments. At pH 4.5, geranium, marigold, and tomato plants reduced the diversity relative to the no plant control. Geranium and tomato plants also reduced diversity at pH 5.5, while petunia plants at 5.5 exhibited the highest diversity across all plant species (p=0.0159). Beta diversity analysis identified pH as the dominant factor explaining 53.7% in Bray-Curtis dissimilarity and 68.5% in the weighted UniFrac distance metrics (both p
Speakers
LC

Laura Chapin

The Ohio State University
Co-authors
MJ

Michelle Jones

The Ohio State University
SN

Sachin Naik

The Ohio State University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Floriculture
  • Subject Floriculture
  • Funding Source NIFA Agriculture and Food Research Initiative, 2022-67013-36489

12:15pm CDT

FRBR - 'CaroRes' and 'EveRes' Series: New Disease-Tolerant Cultivars for Southeastern Peach Production
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Peach (Prunus persica) production in the southeastern United States, particularly in South Carolina, is challenged by high humidity, variable chill accumulation, and persistent disease pressure – especially from bacterial spot (Xanthomonas campestris pv. pruni) adn brown rot (Monilinia spp.). In response, Clemson University reestablished its peach breeding program in 2008 with the goal of developing fresh-market cultivars adapted to this region’s demanding conditions. After more than a decade of field evaluation and selection, three new cultivars have been released under the ‘CaroRes’ and ‘EveRes’ series: ‘CaroRes Wonder’ (USPP 36,072), ‘EveRes Ruby’ (USPP 36,112), and ‘CaroRes Delight’ (USPP approved). The ‘CaroRes’ name signifies both Clemson’s South Carolina origin and disease resistance, while ‘EveRes’ identifies cultivars that combine disease tolerance with a slow-softening phenotype, allowing fruit to remain firm on the tree longer than standard melting types. All three cultivars are vigorous, productive, and well-suited to southeastern U.S. orchard systems. The cultivars exhibit high fruit tolerance to bacterial spot. In replicated trials, they demonstrated reliable productivity and good pack-out percentages with minimal split pit incidence. The fruits are large, round, and clingstone, with light pubescence and a high percentage of red skin blush, ranging from 80% to 95%. All cultivars have yellow melting flesh, though ‘EveRes Ruby’ exhibits a firm, crisp texture at harvest that gradually softens over several days at room temperature, enhancing postharvest handling and shelf life. ‘CaroRes Wonder’ and ‘CaroRes Delight’ ripen in the early season, while ‘EveRes Ruby’ ripens in the early to midseason. Across all three cultivars, fruit quality evaluations consistently rated flavor, texture, and appearance highly. These releases provide new options for growers seeking disease-tolerant cultivars with excellent fruit quality, reliable performance, and adaptability to the environmental challenges of the Southeast. Their unique combination of field resilience, consumer appeal, and handling advantages makes them strong candidates for fresh-market production. Trees are available through licensed TN nurseries, including Cumberland Valley Nurseries and Vaughan Nursery.
Speakers
avatar for Ksenija Gasic

Ksenija Gasic

Clemson University
Co-authors
JM

John Mark Lawton

Clemson University
NA
RB

Ralph Burrell

Clemson University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding
  • Subject Fruit Breeding
  • Poster # csv
  • Funding Source NIFA/USDA-SC-1700568

12:15pm CDT

FRBR - All Gain: Establishing propagation protocols for use of Rubus Allegheniensis in breeding
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
The production of blackberry, a valuable fruit crop, is primarily limited to USDA Zones 5 and higher. However, wild species such as Rubus allegheniensis, R. alumnus, and R. rosa are widespread from Zones 5 through Zone 3a in Minnesota and Wisconsin. Native species are a valuable, untapped genetic resource, which could provide both hardiness for production in colder areas with short growing seasons and resilience to pests, diseases, and climate change for warmer areas. The use of native species for breeding has been limited as little research has been conducted to date. To access the utility of this germplasm, propagation protocols developed for them will need to be established. Because sexual and vegetative propagation requirements for the genus Rubus vary widely depending on taxa and genotype, this research aims to evaluate protocols specific to these species. Canes were collected from 24 northern locations to test rooting of softwood cuttings with either IBA and NAAm auxin treatments in early spring and again in summer. Additional canes were collected from one location to test the rooting of dormant cuttings using IBA and bottom heat to stimulate root growth. Results indicate that the suitability of softwood cuttings for propagation is dependent on the section of the genus, with samples over 30% of samples from section Procumbentes (dewberries) rooting, while sections Arguti, Canadenses, Rubus (highbush blackberries), Setosi (bristleberries), or Ideaobatus (raspberries) all had under 10% rooting rates. In addition, seeds were collected from 5 locations to test germination protocols. Seeds were divided between two stratification treatments(20°C control and a six-month at 4°C). Within those, seeds received either no scarification, physical scarification, 30 minute acid scarification, or 3 hour acid scarification treatments. The 30 minute acid scarified seeds were also divided between a control and aqueous smoke solution treatment. Results from germination tests indicated that long acidic scarification treatments do not result in increased germination at six months.
Speakers
MP

Mike Patrick

University of Minnesota
Co-authors
ET

Erin Treiber

University of Minnesota
NA
MC

Matthew Clark

University of Minnesota
NA
SK

Sarah Kostick

University of Minnestoa
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding
  • Subject Fruit Breeding
  • Poster # csv
  • Funding Source Minnesota Department of Agriculture Specialty Crop Block Grant

12:15pm CDT

FRBR - Assessment of the papaya ringspot virus diversity in South Florida
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Papaya ringspot virus (PRSV) is a devastating pathogen found throughout the tropics and subtropics. Understanding PRSV diversity and evolution is crucial for formulating timely management strategies and breeding resistant cultivars. The molecular epidemiology of PRSV-P was investigated by analyzing the nucleotide sequences of the coat protein gene of different PRSV isolates worldwide, including the sequence data generated by collecting infected samples from South Florida. PRSV samples were collected from different papaya growing regions in Homestead and the wild papaya plantations surrounding Lake Okeechobee. Distinct PRSV clades were identified, corresponding to the U.S. and South American region, Southeast Asia (excluding India), and specifically India. The sequences obtained in the present study showed a close correlation with the aligned with the sequences from Cuba, Mexico, Jamaica, Guatemala, Venezuela, and previous sequences of the Florida isolates. The estimates of PRSV diversity showed an increase in the viral diversity, as the majority of the papaya growing regions are forming a separate clade. However, the evolution is slow, which could be due to the implementation of control strategies and the cultivation of resistant transgenic papayas.
Speakers
SJ

Sumit Jangra

University of Flroida
Co-authors
JS

Jugpreet Singh

University of Florida
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding

12:15pm CDT

FRBR - Identifying an effective method of evaluating blackberry chilling requirement for endodormancy release in Arkansas
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Lack of winter chill accumulation is a major limiting factor for blackberry production in regions with mild or absent winters. For blackberry buds to release endodormancy they must be exposed to a range of temperatures between 0-7°C for a period of time that is genotype-specific. Insufficient chill in blackberries results in incomplete bud development, irregular budbreak, extended flowering periods, nonsynchronous fruit set, and lower fruit yields. The University of Arkansas Division of Agriculture (UADA) Fruit Research Station, located in Clarksville, Arkansas, experiences 1000 hours of annual chill each year, inhibiting the identification of low chill germplasm in the Arkansas Fruit Breeding Program. This study compares two methodologies for determining chilling requirements in six blackberry cultivars (Von, Natchez, Navaho, Ouachita, Sweet-Ark® Ponca, and A-2491T). Long-cane blackberry plants were exposed to controlled artificial chilling in a cooler, with removal at six weekly intervals from 0 to 840 hours, followed by exposure to forcing conditions in a heated greenhouse. Concurrently, field-grown plants of the same cultivars were exposed to naturally occurring chill hours in the field with six stem cuttings per genotype collected biweekly and forced under a mist bed in a heated greenhouse. The number of buds broken, reproductive laterals, and open flowers were recorded on a weekly basis for both methods. Results indicated that both methods effectively differentiated chilling requirements across cultivars, with budbreak and flowering progressing more rapidly and synchronously once plants reached their cultivar-specific chilling requirement. This study aims to identify an effective, repeatable method of evaluating blackberry chilling requirement in Arkansas to inform breeding decisions and identify low chill material in UADA blackberry germplasm.
Speakers
RB

Rhys Brock

University of Arkansas
I am a graduate research assistant of the University of Arkansas Fruit Breeding Program, pursuing my MS in Plant Breeding and Genetics. My research focuses on investigating the diversity of chilling requirement within the University of Arkansas blackberry germplasm.
Co-authors
CJ

Carmen Johns

University of Arkansas
MW

Margaret Worthington

University of Arkansas
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding

12:15pm CDT

FRBR - Identifying Frost-tolerant Southern Highbush Blueberry Cultivars For Alabama
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Blueberry is the second major fruit crop in Alabama, followed by peach. However, blueberry production in Alabama is marginal compared to neighboring states such as Florida, Georgia, and North Carolina. Alabama growers still rely on old rabbiteye cultivars due to a lack of access to southern highbush blueberries (SHB) that are adaptable to the region. SHBs are valued for their early maturation and fruit quality but have not been widely adopted in Alabama due to their susceptibility to spring frost and other possible biotic or abiotic stresses. This project aims to introduce frost-tolerant SHB cultivars in Alabama through multi-environment cultivar evaluation. A total of 12 cultivars and 25 advanced selections of SHB have been evaluated in two locations in Alabama: the E.V. Smith Research Center (EV) in Central Alabama and the Brewton Agricultural Research Unit (BW) in South Alabama. Plants have been evaluated for 50% bloom, average berry weight (g), yield per plant (g), Brix (%), titratable acidity (TA%), and firmness (g/mm). Data from 2024 revealed that San Joaquin (5,035 g) and NC5289 (3,798 g) had the highest average yield at EV. At BW site, TH-2976 (3,375 g) and NC5289 (2,984 g) showed the highest yield. Colossus and Patrecia produced the largest average berry weight for both locations. In the BW, Colossus had an average berry weight of 4.72 g and Patrecia 3.8 g. At the EV location, both Colossus and Patrecia had an average of 3.0 g berry weight. For Brix, FL12-213A (14.7) and NC5326 (14.2) had the highest values at EV, while NC5326 (14.2) and Sentinel (13.8) had the highest values at the BW location. Overall, late-blooming selections such as NC5314 and NC5289 were identified as more suitable for Alabama to mitigate spring frost damage. The frost-tolerant cultivars identified through this project will significantly benefit small to mid-sized growers in Alabama by ensuring stable fruit production.
Speakers
SP

Sakshi Pathania

Auburn University
Co-authors
HA

Hudson Ashrafi

North Carolina State University
NA
JS

Jessica Spencer

North Carolina State University, Horticultural Science
NA
PM

Patricio Munoz

University of Florida
NA
SR

Sushan Ru

Auburn University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding

12:15pm CDT

FRBR - Self-Fertility in Macadamia: Genetic Variation and Mechanisms
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Macadamia is a commercially valuable nut crop, but the global industry is predominantly based on self-infertile cultivars. Pollinator scarcity can result in reduced nut set and lower yields. The development of self-fertile cultivars provides an opportunity to produce consistent yield in the absence of pollinisers and pollinators and may enhance orchard profitability. While previous studies identified varying degrees of self-fertility in macadamia, a comprehensive understanding of genetic diversity within a broad range of germplasm and the mechanisms of this trait remain unexplored. This study involved 11 commercial cultivars to identify genetic variation and mechanisms associated with self-fertility. An outcross pollen exclusion method was employed to assess self fertility. The percentage ratio of self- to open-pollinated nuts were calculated as a measure of self-fertility. To investigate the mechanisms underlying self-fertility, spatial separation of male and female reproductive organs as well as site of pollen tube growth inhibition have been examined. Pistil length, stamen length and stigma-anther distance were measured over two flowering seasons. Fluorescence microscopy was used to observe the pollen tube growth after self-pollinations. The self-fertility ranged between 17%-65% for six cultivars (‘HAES 741’, ‘HAES 791’, ‘A38’, ‘UQM40’, ‘K’, ‘Q’), which were identified as self-fertile. Five cultivars (‘HAES 344’, ‘A16’. ‘Daddow’, ‘D4’, ‘A268’) were identified as self-infertile. Pistil length of self-fertile cultivars ranged from 12.5 to 14 mm. Except for ‘A268’, which had significantly shorter pistils (8.5 mm), the pistil length of the self-infertile cultivars ranged from 11.5 mm to 14.5 mm. Stigma-anther distance was not associated with self-fertility. It was found that all cultivars exhibited similar stigma-anther distance, ranging from of 6 to 8 mm. There was non significant difference between the self-fertile and self-infertile groups for morphological floral traits. Pollen tube was inhibited in the upper third of style in self-incompatible pollinations. This inhibition indicated gametophytic self-incompatibility in macadamia. Further study will use a diverse collection of macadamia germplasm, including commercial cultivars, breeding lines and wild accessions to estimate heritability of self-fertility. Additionally, differential expressed genes in the pistils of self-fertile and self-infertile cultivars will be examined to understand the molecular mechanism regulating the trait. This research will assist in breeding of high-yielding, self-fertile cultivars for future plantations, ultimately supporting the sustainability of macadamia industry.
Speakers
PK

Palakdeep Kaur

Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD 4072, Australia
Co-authors
BT

Bruce Topp

Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD 4072, Australia
NA
KM

Ky Mathews

Department of Primary Industries, Maroochy Research Facility, Nambour, QLD 4560, Australia
NA
MC

max Cowan

Department of Primary Industries, Maroochy Research Facility, Nambour, QLD 4560, Australia
NA
MA

Mobashwer Alam

Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD 4072, Australia
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding

12:15pm CDT

FRBR - Utilizing SPME-GCMS to Investigate Aroma Components of Black, Purple, and Red Raspberry (Rubus occidentalis, R. idaeus x occidentalis, and R. idaeus)
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Black raspberry (Rubus occidentalis) and purple raspberry (R. idaeus x occidentalis) are fruits known for their pleasant and distinctive flavors. However, unlike their more popular cousin red raspberry (R. idaeus), little is known about what defines the characteristic aromas of black and purple raspberry. Using SPME-GCMS, the chemical compounds in the headspace of pureed black raspberry (4 genotypes), purple raspberry (5 genotypes), and red raspberry (2 genotypes) were collected and identified through a combination of gas chromatography and mass spectrometry. Chemical concentrations were calculated by integrating the area under the chromatogram curve, and calibrated with an internal standard of 2-octanol. By utilizing a random forest model, chemicals that were the most important for distinguishing fruit types (red vs. black vs. purple) were determined. Of the top 30 chemicals, 12 chemicals were described on Flavornet as detectable by a human nose in gas chromatography-olfactometry (GC-O), and the typical aroma described. Those 12 chemicals were then evaluated individually for significant differences across types (red, black, and purple raspberry). Among the chemicals that differed significantly across species were D-Carvone (caraway aroma), 1,3,8-p-Menthatriene (turpentine aroma); β-Ionone (violet, flower, raspberry, and seaweed aromas); α-Ionone (violet aroma); 2-Hexenol (leaf, green, wine, and fruit aromas); and α-Terpineol (oil, anise, and mint aromas). These results have implications for breeding new black and purple cultivars with the expected characteristic flavor. This instrumental approach also allows for tracking these aromas in breeding populations when utilizing interspecific hybridization to broaden the black raspberry germplasm in breeding.
Speakers
LW

Lena Wilson

Cornell University
Co-authors
CW

Courtney Weber

Cornell University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding

12:15pm CDT

FRBR - Validation of Diagnostic Markers for Seedlessness and Flower Sex in Diverse Muscadinia and Vitis Grapes
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
The Vitis genus consists of two subgenera, Vitis and Muscadinia, which are both cultivated for wine, juice and fresh consumption. Fresh market breeding programs for grapes (V. vinifera) and muscadines (M. rotundifolia) aim to develop stenospermocarpic seedless and perfect-flowered cultivars. Despite chromosome differences and genetic divergence between the Vitis (2n=38) and Muscadinia (2=40) subgenera, stenospermocarpic seedlessness from V. vinifera was successfully introgressed into M. rotundifolia through conventional breeding. Currently, molecular markers for stenospermocarpy have not been developed or validated in Muscadinia and diverse Vitis accessions. Before the divergence of the Vitis and Muscadinia subgenera, two mutations occurred in perfect flowered ancestors, leading to male and female sterility and resulting dioecy in wild plants from both subgenera. Kompetitive Allele Specific PCR (KASP) markers have been recently developed targeting the known causal mutations resulting in male sterility (VviINP1) and stenospermocarpy (VviAGL11) in Vitis. The markers named, seedless_Arg197Leu_site56.fas and female_INP_indel_site56.fas, were evaluated for their diagnostic potential across Vitis and Muscadinia species through sequence alignments with published genomes. The predictive ability of these markers was assessed using a validation panel from the University of Arkansas Division of Agriculture Fruit Breeding program, which included 918 seedlings from Vitis x Muscadinia hybrid breeding populations. Additionally, a range of diverse Vitis and Muscadinia accessions with known phenotypes were included to enhance understanding of marker effectiveness, including cultivars, selections, and wild material from USDA germplasm repositories and public and private breeding programs. In total, 209 diverse accessions were assessed with the seedless marker, and 320 accessions were evaluated with the flower sex marker. The evaluation for seedlessness and flower sex took place over 2023 and 2024. Excluding incomplete phenotype and genotype data, the stenospermocarpic marker (seedless_Arg197Leu_site56.fas) accurately predicted seedlessness in 921 of 924 entries. Additionally, 148 out of 203 seedlings that did not produce fruit in both growing seasons were predicted to be stenospermocarpic. A t-test comparing vine caliper between seeded and seedless material (P =0.178) showed no difference in vigor between the seeded and seedless vine and suggested that the high number of seedless vines with no fruit may be caused by sterility factors linked to the introgressed Vitis stenospermocarpy locus. The flower sex marker (female_INP_indel_site56.fas) correctly predicted flower sex in 1,137 of 1,138 entries. Overall, the KASP markers showed outstanding predictive performance, achieving accuracy rates of 99.9% for flower sex and 99.7% for seedlessness across Vitis, Muscadinia, and hybrid germplasm.
Speakers
IV

Isabella Vaughn

University of Arkansas
Co-authors
CJ

Carmen Johns

University of Arkansas
CZ

Cheng Zou

Cornell University
NA
CH

Claire Heinitz

USDA ARS
NA
LN

Lacy Nelson

University of Arkansas
NA
LC

Lance Cadle-Davidson

USDA-ARS Grape Genetics Research Unit
NA
MW

Margaret Worthington

University of Arkansas
QS

Qi Sun

Cornell University
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding
  • Subject Fruit Breeding
  • Poster # csv
  • Funding Source This work was funded by VitisGen3, which was provided by Specialty Crop Research Initiative Competitive Grant, Award No. 2022-51181-38240, of the USDA National Institute of Food and Agriculture.
  • Funding Option SCRI funded all or part of the research associated with this abstract

12:15pm CDT

FRBR - ‘Ga. 6-1-269’, A Bronze, Fresh-Fruit Muscadine Grape with Hermaphroditic Flowers and Large Berries.
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
'Ga. 6-1-269' is an hermaphroditic muscadine grape (Vitis rotundifolia) with bronze berries that was released by the University of Georgia (UGA) College of Agriculture and Environmental Sciences. Yields of ‘Ga. 6-1-269’ in Tifton, GA and Citra, FL trials were similar to other popular fresh-market muscadine cultivars. Berry size is very large (15-16 g) and similar to the cultivars 'Ga. 6-2-26' (Paulk®) and 'Supreme'. Fruit rot susceptibility of ‘Ga. 6-1-269’ was less than the bronze check cultivar 'Ga. 1-1-48' (Hall®) and similar to the black check cultivars 'Ga. 6-2-26' and 'Supreme'. Harvest time of ‘Ga. 6-1-269’ was about 5 days after 'Ga. 1-1-48' and about a week before 'Ga. 6-2-26' and 'Supreme'. ‘Ga. 6-1-269’ is recommended as an early-midseason fresh market bronze muscadine cultivar.
Speakers
PC

Patrick Conner

Professor, University of Georgia
Co-authors
AS

Ali Sarkhosh

University of Florida
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Fruit Breeding

12:15pm CDT

PNM 1 - Effect of different levels of magnesium fertilization on carotenoid accumulation in three varieties of kale grown hydroponically
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Kale (Brassica oleracea L. var. acephala) is a highly nutritious cruciferous vegetable valued for its abundant phytochemicals, particularly carotenoids such as β-carotene, lutein, and zeaxanthin. These compounds play crucial roles in human health by acting as antioxidants and precursors to vitamin A, aiding in the prevention of non-communicable diseases such as cancer, cardiovascular disorders, and age-related macular degeneration. Despite the health benefits of carotenoids, their concentration in kale can be significantly influenced by environmental and agronomic factors, particularly nutrient availability. Magnesium (Mg), an essential macronutrient and a core component of the chlorophyll molecule, plays a pivotal role in photosynthesis, enzyme activation, and nutrient translocation. While its role in general plant metabolism is well established, the impact of magnesium fertilization on carotenoid accumulation in kale remains underexplored. This study investigates the effect of different magnesium fertilization rates (12.3 mg/L, 24.6 mg/L, 36.9 mg/L, and 49.2 mg/L) on the accumulation of carotenoids and elemental nutrients in three kale cultivars—Darkibor, Mamba, and Red Russian—under hydroponic conditions. A split-plot experimental design will be implemented in a greenhouse using an NFT (Nutrient Film Technique) system. The research will evaluate chlorophyll (‘a’ and ‘b’) contents in the leaves, fresh and dry biomass, crop height, crown diameters, elemental nutrient concentrations using the ICP mass spectroscopy method, and carotenoid levels through HPLC analysis. The results will be statistically analyzed using ANOVA in RStudio. This study seeks to identify optimal magnesium application rates that enhance the nutritional quality of kale, providing insights into sustainable fertilization strategies for maximizing the health benefits of this functional crop.
Speakers
avatar for Alex Kofi

Alex Kofi

Graduate Research Assistance, Kentucky State University
Graduate Research AssistantResearch interest: Controlled Environment Agriculture especially in hydroponics
Co-authors
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

PNM 1 - Effects of Magnesium Fertilization on Vitamin C Accumulation in Watercress Grown Hydroponically
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Watercress (Nasturtium officinale L.) is a leafy green vegetable, that is a member of the Brassicaceae family. It provides a rich and bioavailable source of vitamin C, significantly higher than many fruits and vegetables, including oranges. Vitamin C is associated with numerous health benefits, such as antioxidant protection, immune system support, enhanced collagen production, improved iron absorption, improved eye health and lowered risks of chronic diseases such as cardiovascular disease and cancer. Thus, consuming adequate amounts of vitamin C daily is important for human health. A 100g of watercress provides 62mg (103% Recommended Dietary Allowance) of vitamin C. In humans, vitamin C takes part in various physiological processes; however, due to the functional loss of the gene coding for L-gulonolactone oxidase, humans cannot synthesize vitamin C and must rely primarily on plant-based foods for their needs. For this reason, increasing the vitamin C content of crops is essential for supporting human health. Some studies have shown potential effects of fertilization, particularly magnesium fertilization on vitamin C content. Therefore, this study aims to contribute to optimization of nutrient management in hydroponics to enhance nutritional value. The research was conducted using a split-plot design. Four magnesium fertilization rates (100 mg/l, 150 mg/l, 200 mg/l and 250 mg/l) were applied to watercress in Nutrient Film Technique (NFT) hydroponic system under controlled environment, ensuring desirable nutrient application, temperature, light, pH and electrical conductivity. Spectrophotometric methods were used to quantify Vitamin C. The preliminary findings indicate an increase in Vitamin C content with an increase in magnesium application rates, with the highest rate maximizing its accumulation. These results contribute to understanding the effects of magnesium fertilization on vitamin C synthesis in watercress and will help guide farmers and producers in optimizing vitamin C content during production to support human dietary needs and health. Keywords: Watercress, Vitamin C, Magnesium Fertilization, Hydroponics, Nutrient Optimization
Speakers
MM

Marie Merci UWIMBABAZI

Kentucky State University
Co-authors
CT

Cora Teets

Kentucky State University
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

PNM 1 - Impact of Nitrogen Sources and Rates on Tea (Camellia sinensis) Transplant Growth and Soil pH in A Silty Upland Soil
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Two nitrogen (N) sources – synthetic urea ammonium sulfate (UAS, 33-0-0) and organic bloodmeal (BM, 13-0-0) were evaluated for growing tea transplants in southeastern Louisiana. In Expt. 1, UAS and BM were applied at three rates (150, 250, and 350 lbs. N/A/year) to a 1-year-old tea field. Expt. 2 evaluated five treatments by substituting UAS with BM at 0%, 25%, 50%, 75%, and 100%, all at 350 lbs. N/A/year, applied to a 2-year-old tea field. N applications were divided into seven applications and applied every 40 days from April to October in 2023 and 2024 for a total of 11 applications. Despite periodic pruning to encourage branching for the building of a plucking table, plant growth metrics, such as leaf greenness and size index were similar across treatments in both experiments. However, tissue nitrogen concentration (N%) was consistently higher in BM-treated plants compared to UAS-treated plants at all application rates in Experiment 1. The highest tissue N% was also observed in the 100% BM treatment in Experiment 2. One year after treatment initiation, plants fertilized with UAS exhibited faster recovery after pruning compared to those treated with BM, though this difference diminished over time. In both experiments, plots treated with blood meal or more than 75% blood meal replacement resulted in less acidification compared with those treated with UAS. Further research on leaf quality (e.g., health-promoting compounds) and a more comprehensive evaluation of soil microbial activity are required to refine recommendations for nitrogen application rates and sources for tea production.
Speakers
AH

Autumn Hilderbrand

Louisiana State University
NA
Co-authors
JW

Jim Wang

Louisiana State University
NA
YC

Yan Chen

LSU AgCenter
ZX

Zhimin Xu

Louisiana State University School of Nutrition and Food Sciences
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Nutrient Management 2

12:15pm CDT

PNM 1 - Multi-Species Cover Crop With Legumes Consistently Produced High Biomass Compared To Mustard Only Blend Or Grass Only In California Walnut Orchard
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Planting a cover crop in orchard middles can have many benefits for the health of the trees. In California, improved water infiltration, especially during the winter rainy season, is a common motivation for cover cropping, and the benefit to soil health through increased biological activity resulting from biomass incorporation is also gaining renewed attention. Cover crops can also play a part in nutrient management by “mining” and holding existing nutrients, especially nitrogen, at the end of the season and, when legumes are included, by direct sequestration of nitrogen from the atmosphere through the symbiosis of their roots with nitrogen-fixing bacteria. Different cover crop types can contribute in different ways to these outcomes, but benefits depend on successful establishment and a significant amount of biomass being produced. We evaluated three common cover crop types over three years in a walnut orchard in northwestern San Joaquin valley with a heavy clay-loam soil. Treatments included a pure stand of winter triticale, a ‘pollinator’ mustard mix (multiple Brassica spp. plus Raphanus sativus [Daikon]), and a multi-species mix including grain rye and triticale, mustards (Sinapis alba and Raphanus sativus), and legumes (Vicia faba, Vicia sativa, Lathyrus oleraceus). Treatments were planted with a seed drill in two adjacent orchard middles at two parts of the orchard in early Nov. 2022, 2023, and 2024 and depended on rain for germination and growth. In 2023 and 2024, unplanted controls were maintained in rows adjacent to the trial rows. Sampling plots were defined at five representative points in the planted rows. Just before cover crop termination early in the April following each planting, above ground vegetation was sampled using a one-meter quadrat. While triticale and the mustard blend produced above ground dry biomass at 0.13 to 0.28 and 0.04 to 0.27 kg/sq. m, respectively, the multi-species mix consistently produced three to five times that amount, ranging from 0.53 to 0.68 kg/sq. m. Analysis of soil sampled one week before cover crop termination in 2025 did not show significant differences in organic matter, N, P, and K content, but samples of chopped cover crop taken at termination showed plant N content twice as high in the multi-mix treatment than in the others. This trial has highlighted the resilience of planting a diverse plant mix in comparison to more homogeneous cover crop types, and the advantage of including legumes when N may be a limiting factor to cover crop biomass growth.
Speakers
avatar for Kamyar Aram

Kamyar Aram

Specialty Crops Advisor, UC Agriculture and Natural Resources
UC Cooperative Extension Advisor for Alameda and Contra Costa Counties, serving production horticulture. Current work focuses on IPM and biocontrol, cover crops, and irrigation management.
Co-authors
TJ

Tom Johnson

seeds for bees, Project apis m.
NA
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Nutrient Management 2

12:15pm CDT

PNM 1 - The Effect of Magnesium Fertilization on Glucosinolate Accumulation and Leaf Biomass in a Cress Variety
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
The leafy greens have generated significant worldwide interest due to their nutritional quality. Cress is among the leafy greens that are known for their nutrient-dense, phytonutrient content. Upland cress (Barbarea verna) is among the Cress varieties with tender greens known for their vibrant flavor and impressive nutritional profile. It possesses high metabolic activity, which enables it to synthesize a rich variety of phytonutrients. Magnesium, as a macronutrient, is known for influencing the biosynthesis of plant metabolites, including the glucosinolates biosynthesis pathway. The high accumulation of glucosinolate in upland cress could not only increase its nutritional quality but also market demand. The study assessed by supplementing magnesium fertilization rate on upland cress grown under a Nutrient Film Technique (NFT) system to identify the magnesium concentration that led to maximum accumulation of glucosinolate and biomass in upland cress. The split plot design was used, where four treatments of magnesium fertilization rates (100 mg/l, 150 mg/l, 200 mg/l, and 250 mg/l) were used. All treatments were under a controlled environment, ensuring nutrient application, temperature, light, pH, and electrical conductivity are in a good range required for Upland cress. After two weeks of germination, the Upland cress reached its maturity stage, and samples for leaf biomass were collected, and High-performance liquid Chromatography (HPLC) was employed for glucosinolate analysis. Preliminary findings showed that the magnesium fertilization rate of 150 mg/l and 200 mg/l stimulated high accumulation of leaf biomass and glucosinolate in upland cress, and the research is still ongoing. Keywords: Upland cress, Magnesium fertilization, leaf biomass, nutritional quality, Hydroponics.
Speakers
EM

Eric Murwanashyaka

kentucky state university
Co-authors
RK

Richard Kabanda

Kentucky State University
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Tuesday July 29, 2025 12:15pm - 1:00pm CDT
Empire AB

1:00pm CDT

CE 1 - A Comparative Analysis of Root Morphology in Various Spinach (Spinacea oleraceae) Varieties: Field vs Hydroponic Growth
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Spinach (Spinacea oleraceae) is one of several vegetable crops commercially grown in Controlled Environment Agriculture (CEA). Recent research has indicated differences in root morphology and architecture among various crops may influence performance and environmental stressor tolerance. Specifically, fast-growing cultivars have shorter life cycles, develop more extensive and specialized root systems with increased root order differentiation in response to their growth systems. Contrastingly, slow-growing cultivars have greater heat-tolerance and produce simpler root systems with less root-order differentiation and fibrous roots. These root traits may be exploited to increase yield, promote efficient nutrient use, and mitigate environmental stressors. Novel studies have revealed an ideal root system archetype, known as “Subsoil Foraging Ideotype (SFI)”, wherein a well-penetrating taproot is able to easily maintain nutrient and water flow while optimizing resource efficient root development. We designed an experiment to measure differences between various spinach cultivars in CEA systems to reveal morphological variation which can aid in the development of SFI architecture cultivars. Our experiment utilized two growth systems located in a walk-in greenhouse, standalone NFT hydroponic systems with two inch net pots and expanded clay aggregate media, and one gallon pots to replicate field conditions. Seeds from fast-growing “Income”, medium-growing “Darkside” and slow-growing “El-Majestic” cultivars were transplanted into different growing systems. Supplemental light totalling 200 µmole/m2/s with 12 hour photoperiod and periodic fertigation was used. Harvests were conducted on days 15, 30, and 45, with the collection of leaf and root biomass. Collected data included scanning of root systems in WinRhizo software, measuring length, root volume, root diameter, surface area, classification by diameter, forks, tips, and crossing numbers. On day 45, four plants per cultivar were selected for anatomical analysis through microscopy evaluation and underwent JB4 embedding protocol. Anatomical analysis of five root orders included diameter, vascular development, and rhizodermis characteristics. Preliminary results suggest there is an interaction effect between cultivar, growth system, and developmental stage on spinach root morphology. All cultivars developed more extensive, specialized root systems under hydroponic conditions versus field counterparts where extensive root development was limited to fast-growing cultivar. Root diameter was greater in fast-growing cultivars, with decreased root order diversification of slow and medium-growth cultivars. These results affirm previous research which links root morphology to growth systems and environmental conditions. Cultivars which possess resilient traits may be further developed in conjunction with SFI archetypes. Such advances would further nutrient use efficiency and shrink growth cycles in CEA.
Speakers
DC

Deniz Camli-Saunders

University of Rhode Island
Co-authors
CV

Camilo Villouta

University of Rhode Island
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 4

1:00pm CDT

CE 1 - Co-Culturing Lettuce and Freshwater Prawns in Three Production Systems: A Comparative Study of Coupled Aquaponics, De-coupled Aquaponics, and Aquaculture+Hydroponics
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
This study evaluated the growth and yield performance of two lettuce cultivars (Lactuca sativa L. cv. Green Forest’ and ‘Rouxai’) and freshwater prawn (Macrobrachium rosenbergii) across three production systems: coupled aquaponics (CA), de-coupled aquaponics (DA), and aquaculture hydroponics (AH). In the CA systems, water continuously recirculated between a hydroponic tank (HT), aquaculture tank (AT), and biofilter. In the DA systems, the HT remained separate from the AT and biofilter, but water was occasionally transferred from the AT to the HT. The AT and biofilter were also separated from the HT in AH systems, with no solution intermixing. Our study, conducted in a high tunnel supplied with municipal water (Texas A
Speakers
SC

Seunghyun Choi

Postdoctoral research associate, Texas A&M Agrilife Research
Co-authors
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 4

1:00pm CDT

CE 1 - End-of-Production Lighting Effects on Spinach (Spinacia oleracea) Growth and Nutritional Quality
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Spinach is a commonly grown greenhouse crop, thus production practices have been honed over the years. However, additional strategies to improve production efficiency, yield, appearance, and nutritional quality are needed to continue improving the long-term feasibility of production. Light is a major environmental factor that influences the growth and quality of spinach, however, adding supplemental light is energy intensive and can be costly. A strategy to minimize electrical use while increasing crop quality is end-of-production (EOP) lighting where the light provided to the plant is altered just before harvest and not throughout production. The objective of this research was to determine the effect of EOP light intensity, quality, and duration on the growth and post-harvest characteristics of spinach ‘Space’ (Spinacia oleracea). Propagation was 14 days; then seedlings were transplanted into deep-water culture systems and grown for an additional 21 days in a common greenhouse environment. During production, HPS supplemental lighting was used to create a 16-h photoperiod and a daily light integral of 9.0 ± 2.3 mol·m−2·s−1. Seven, 3, and 1 day before harvest, broad-spectrum white LEDs [17:40:43 R:G:B] provided additional light intensities of 0 (control), 100, 200, 400, and 600 µmol·m−2·s−1, and red/blue LEDs [53:1:46 R:G:B] provided 200 µmol·m−2·s−1 for a 16-h photoperiod. At harvest, growth and morphology including fresh and dry mass, plant height and width, and leaf number and nutritional quality including B and C vitamin and carotenoid concentrations were quantified. Post-harvest evaluation was conducted for 3 weeks; fresh mass, color, and nutritional quality were quantified and qualitative appearance data was collected. These results provide insight on how to effectively leverage light intensity, quality, and duration at the EOP to improve the growth and nutritional quality of hydroponic greenhouse-grown spinach.
Speakers
JF

Jacob Friedrich

University of Tennessee Knoxville
Co-authors
GO

Garrett Owen

The Ohio State University
KW

Kellie Walters

University of Tennessee Knoxville
NA
SA

Sarah Armstrong

University of Tennessee Knoxville
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 4
  • Poster # csv
  • Funding Source This work is supported by the Agriculture and Food Research Initiative, project award no. 2023-68013-39640, from the U.S. Department of Agriculture’s National Institute of Food and Agriculture.

1:00pm CDT

CE 1 - Impacts of far-red supplementation and substitution treatments on the morphology and growth of three Brassica microgreens
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Microgreens are produced in controlled environments under relatively low light intensities (
Speakers
AP

Anthony Percival

Colorado State University
Co-authors
JC

Joshua Craver

Colorado State University
OF

Oliver Fulton

Colorado State University
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

CE 1 - Using Manure-Amended Media to Grow Lettuce in a CEA System
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Livestock and poultry industries have expanded significantly to meet the food requirements of the rapidly growing global population resulting in the generation of almost 1.4 billion tons of manure annually in the United States. To establish innovative approaches to decrease these manure stockpiles, we investigated the potential of using composted manures as effective nutrient-rich amendments for vegetable crops in a controlled environment agriculture (CEA) system. Lettuce (Lactuca sativa L. in the Asteraceae family), one of the most important leafy vegetables, grows well in the field or a CEA system. Furthermore, it can grow under passive or active hydroponic systems. Therefore, in 2024, a project was established to evaluate an innovative solution for the application of composted manures for horticultural crops and their interaction with soil properties in controlled environment conditions in a greenhouse at the Derrickson Agricultural Complex (DAC) at Morehead State University, Kentucky. Three types of composted manures (sheep, cow, and horse) with and without zeolite (crystalline hydrated aluminosilicate) were added in various proportions to a commercial media (Pro-Mix BX All Purpose Growing Mix) and used as the growing media for seedlings of two Romaine lettuce cultivars (Salvius and Amadeus) in six-inch pots, sub-irrigated, and grown until harvesting in the greenhouse. Data on plant morphological and physiological characteristics were collected. The lettuce heads were harvested after two months, and fresh and dried yields were determined. The leaf tissue and media were analyzed for chemical nutrients. The initial results of the first season show significant differences among cultivars and media.
Speakers
AR

Alireza Rahemi

Morehead State University
Co-authors
AS

Amy S. Collick

Morehead State University
NA
CR

Caroline Roush

Morehead State University
NA
HM

Hudson M. Cox

Morehead State University
NA
LN

Larry N. Goble

Morehead State University
NA
RC

Ricky C. Young

Morehead State University
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

ORGH 1 - Heart-shaped Tomatoes: Identifying Varieties with High Fruit Quality and Yield in a Short Growing Season
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Tomatoes are a highly prized crop all over the U.S, but consumers are seeking more flavorful and high-quality produce. Tomatoes are also popular with growers and consumers in colder regions as well. Short and cold growing seasons provide a significant challenge to tomato production while most breeding and research on tomatoes has been done in warmer climates. In an effort to provide research relevant to growers and breeders in colder regions, this project conducts a germplasm screening of over 80 heart-shaped, indeterminate tomato varieties, many of which were bred in colder regions, in order to provide information on specialty, open-pollinated tomatoes that may be better suited for a shorter season. These tomatoes are characterized by their large size, heart-shape, fleshiness, and fewer seeds. Varieties were obtained from a number of seed catalogs as well as from the USDA Germplasm Repository. In the first year of the project, the varieties were screened for yield, earliness and fruit size measurements as well as dry matter, Brix, pH, and titratable acidity. Genotypes were planted in an augmented design and managed organically in an unheated high tunnel. Based on this year of data, some of the high yielding varieties with the mentioned satisfactory quality traits included ‘German Red Strawberry’, ‘Cuore di Toro’, ‘Russian 117’, ‘Upstate Oxheart’, and a GRIN variety ‘G32329’. None of the varieties exceeded acceptable levels of acidity, but there was much variability in both yield and Brix within the experiment. We also found that a number of varieties categorized as “heart-shaped” were not morphologically heart-shaped. This experiment will be conducted over another growing season in order to collect further data and insights on this project.
Speakers
SF

Svetlana Frost

Montana State University
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 2

1:00pm CDT

ORGH 1 - Impact of Biochar Amendment on Growth and Root-Associated Microbial Communities in Day-Neutral Strawberries
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Strawberry (Fragaria × ananassa) production has grown significantly in recent decades, increasing demand for specialty crops. Despite improvements in management practices and breeding, maintaining soil health and minimizing environmental impact remain a challenge for agricultural systems. Biochar production and application have been proposed as one effective strategy to mitigate climate change, improve soil health, and promote plant growth. This study, done at the University of Maryland Eastern Shore (Princess Anne, MD), investigated the effect of biochar on the growth and root associated microbial communities of two selected day-neutral strawberries, Monterey and Seascape, under greenhouse conditions. A pot experiment was conducted with three treatments: No biochar (control), 5% biochar, and 10% biochar. Growth parameters including plant height, number of leaves, number of flowers, runner production, and chlorophyll content were measured at 30, 60 and 90 days after transplanting. The 5% biochar treatment enhanced leaf development, runner production, and yield in Monterey, whereas biochar at 10% improved chlorophyll content in both cultivars. The microbial analysis revealed higher Amplicon Sequence Variants (ASVs) with 10% biochar. Cyanobacteria and Proteobacteria were the dominant microbial taxa across all treatments at the phylum level, with no significant differences between biochar treated groups and control. Alpha diversity metrics showed no significant differences (p > 0.05) between treatments, whereas the beta diversity showed a slight shift in microbial community composition in the biochar amendments. A more diverse microbial community was observed in the control group, nevertheless, the biochar amendment demonstrated a more stable and functionally enriched microbiome. These findings contribute to the growing body of knowledge on biochar’s role in optimizing crop production and supporting sustainable farming practices.
Speakers
EA

Erasmus Aduteye

University Of Maryland Eastern Sh
Co-authors
ND

Naveen Dixit

University of MD Eastern Shore
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 2
  • Subject Organic
  • Poster # csv
  • Funding Source University of Maryland Eastern Shore Extension

1:00pm CDT

ORGH 1 - Impacts of Local Effective Microorganisms on Organic Vegetable Systems in Georgia: Soil Nitrogen Dynamics, Crop Yields and Produce Quality
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
As our understanding of the microbiome’s importance to agriculture improves, questions surrounding effective microorganism inoculants as crop treatments continue to arise. These products purportedly increase nutrient bioavailability and enhance plant growth. However, these vary in contents by producer and can be costly, especially given the inconsistent results on their efficacy. Local effective microorganisms (LEM) are alternative formulations produced with local forest litter and carbon sources that can be produced at scale on-site by farmers using local ingredients. LEM application has previously been shown to alter the dynamics of nitrogen availability and soil microbial populations when added to soil amendments, and may influence crop quality and microbial community resilience. However, long-term research on its relevance to organic vegetable rotations is still in its early stages. This project sought to determine the impacts of LEM application on the yield and quality of organic vegetable crops, and to compare soil nitrogen dynamics of these systems to a controlled laboratory incubation. In the field component, a vegetable rotation consisting of kale, carrot, or crimson clover (spring) and green beans (fall) was grown at three different organic farms in the Georgia Piedmont region for two years, and received either a control, low, or high LEM application rate twice per year. Soil samples were taken periodically for inorganic nitrogen, and at harvest total and marketable yields were taken. Subsamples of each harvested plot were juiced and analyzed for sugar content via %Brix. The laboratory incubation was performed on samples obtained from each plot from the field study, which were incubated for 120 days at a standard water content. Each microcosm received either no treatment or an LEM treatment analogous to its respective field plot at time zero. Sulfuric acid traps were used to measure ammonia volatilization, and samples were periodically taken from each microcosm to be analyzed for inorganic nitrogen and pH. At Day 28 of incubation, the average total inorganic nitrogen across treatments was 5.68 ppm. At the end of incubation, the average pH across all treatments was 5.97. The average %Brix for the beans across all locations was 3.5; plants that received high LEM showed higher Brix in plots where crimson clover was the winter crop in two of the three locations, and in plots where kale was the winter crop in one of the locations. These results indicate that LEM may influence produce quality metrics in some common vegetable crops.
Speakers
MJ

Micah Jones

University of Georgia
Co-authors
DF

Dorcas Franklin

University of Georgia
NA
KC

Kate Cassity-Duffey

University of Georgia
MU

Muhammad Usman Khalid

University of Georgia
NA
SN

Sultan Nawaz

University of Georgia
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 2
  • Subject Organic
  • Poster # csv
  • Funding Source SARE

1:00pm CDT

ORGH 1 - Influence of planting date and fertilizer rate on organic water spinach in high tunnel conditions
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Water spinach (Ipomoea aquatica) is classified as a noxious weed by the USDA. However, it remains a popular vegetable in Southeast Asia. Water spinach thrives in warm, moist conditions. Demand for water spinach is increasing in the state of Georgia. The present study assesses the effects of planting date and organic fertilization rate on water spinach yield. The study was conducted in an organically certified high tunnel at the University of Georgia Tifton Campus. Organic fertilizer was applied and incorporated into the soil before planting. Water spinach seedlings were grown in a greenhouse. Within the high tunnel, plants were cultivated on drip-irrigated raised beds (1.8 m apart center to center), covered with white-on-black film mulch. Plants were grown in two rows per bed, with 45 cm between rows and 30 cm between plants. The experimental design was a split-plot arrangement with three replications. The main plots consisted of three planting dates—T1 (16 May), T2 (9 July), and T3 (3 Oct.)—while the subplots included four fertilization rates: 0, 56, 112, and 224 kg/ha of nitrogen (N) applied as organic fertilizer. Plants (shoots) were harvested by cutting 5 cm above the soil surface: four times for T1, three times for T2, and once for T3, with the final harvest conducted on 22 Nov. Shoot fresh weights (FW) were recorded. Results showed that cumulative shoot FW was highest at the earliest planting date (T1; 2.77 kg/m²), followed by T2 (2.36 kg/m²), and lowest at the latest planting date (T3; 0.062 kg/m²). The reduced cumulative shoot fresh weight observed in the latest planting suggests that cooler fall conditions significantly limited plant growth. Regarding the fertilizer rate, the cumulative shoot FW was highest at the 224 kg/ha N fertilization rate (2.03 kg/m²) and lowest at 0 kg/ha N (1.41 kg/m²). However, the relatively high shoot growth at 0 kg/ha N is notable and may indicate the presence of confounding factors. Plants exhibited vigorous shoot and root development, including the formation of adventitious shoots, which likely allowed them to explore soil beyond the experimental plot boundaries (the separation between plots was 30 cm). Visually, plants appeared to grow more actively when provided with ample soil moisture and organic fertilizer. In conclusion, the optimal planting window for water spinach in South Georgia appears to be from April to July. The effect of fertilizer rate on water spinach growth could not be conclusively determined from this study.
Speakers
JC

Juan Carlos Diaz Perez

University of Georgia
Co-authors
DP

Dharma Pitchay

Agricultural Sciences and Engineering, Tennessee State University
GG

Gunawati Gunawan

University of Georgia
TM

Ted McAvoy

University of Georgia
NA
TC

Timothy Coolong

University of Georgia
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

ORGH 1 - Natural Variation and Genome-Wide Association Mapping of Photosynthetic Traits in USDA Spinach Germplasm Under Organic Production
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Sustainable agricultural practices are essential to mitigating the impact of climate-induced stresses on crop production. Enhancing photosynthetic efficiency is a key strategy to boost yield, productivity, and resilience to stress, especially in organic farming. This study aimed to identify natural variation in leaf photosynthesis and uncover key genetic regulators of physiological and molecular responses in USDA spinach (Spinacia oleracea) germplasm under organic cultivation. 314 USDA organic spinach accessions and commercial checks were planted in an augmented design within an organic field in Uvalde, Texas. Gas exchange traits—including CO2 assimilation rate (A), carboxylation efficiency (CE), CO2 concentration in leaf air spaces (Ci), transpiration (E), CO2 efflux, stomatal conductance (gsw), and water use efficiency (WUEi)—as well as chlorophyll fluorescence metrics such as the efficiency of energy harvesting by oxidized (open) PSII reaction centers (Fv’/Fm’), quantum yield of PSII, electron transport rate, non-photochemical quenching, and photochemical quenching were measured using the LI-6800 portable photosynthesis system. The genome-wide association study (GWAS) was conducted on photosynthesis traits in 299 spinach accessions using 50,873 SNPs. Several SNP markers associated with different traits and candidate genes were identified. Our findings emphasize the value of combining high-throughput photosynthesis measurements with GWAS to reveal the genetic basis of photosynthetic variation in crop species.
Speakers Co-authors
AS

Ainong Shi

University of Arkasnas
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 2
  • Subject Organic
  • Poster # csv
  • Funding Source the Organic Agriculture Research and Extension Initiative (OREI USDA-NIFA) Federal Award # 2022-51300-37886
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

1:00pm CDT

ORGH 1 - On-Farm Participatory Research to Determine Organic Nutrient and Microbial Spray Efficacy in Increasing Disease Resistance
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Obtaining sufficient nutrients and fighting foliar diseases caused by pathogens such as Alternaria and Cercospora remain an on-going problem for many carrot (Daucus carota) growers. Spraying leaves with mixtures containing soluble nutrient sources and beneficial microbes have potential to help address these issues, but the benefits may depend on the responsiveness of individual carrot genotypes. To test this hypothesis, a field trial was conducted on an organic farm in northern Indiana using three diverse carrot genotypes (Napoli, Nb3999, and Bolero). Each genotype was sprayed with a mixture of four commercial products commonly used by many organic farmers in the area, or left untreated as a control. Soil samples were collected midseason and changes in soil chemical and biological properties were quantified using standard practices. The incidence and severity of foliar diseases was also evaluated visually during the growing season and leaf samples were collected for quantification of leaf microbiomes. At harvest, total shoot and root biomass was determined and carrot taproot samples were collected for analysis of nutritional quality and endophytic microbiomes. Preliminary results indicated that foliar diseases were not particularly problematic during the 2024 growing on this farm. The carrot genotypes varied significantly with respect to above and belowground biomass, however, the treatments did not have any effect on these parameters. There were also no differences in the impact of the foliar sprays on soil parameters, which was expected. We suspect that the lack of any effect of the foliar sprays on carrot biomass was due to the absence of disease pressure at this site, where changes induced in leaf and root microbiomes could have helped mitigate any disease pressure.
Speakers
KQ

Kayla Quintero

Purdue University
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 2
  • Subject Organic
  • Poster # csv
  • Funding Source Carrot Improvement for Organic Agriculture (CIOA)
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

1:00pm CDT

ORGH 1 - Optimizing the use of dry media amendments to enhance vegetable transplant growth in organic production systems
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Vegetable transplant production is a critical phase that enhances the efficiency, sustainability, and profitability of vegetable cropping systems. In the U.S. Midwest, many organic vegetable growers produce their own transplants due to the limited commercial availability of certified organic transplants. A major constraint in this process is managing nutrient availability, which can compromise transplant quality. While a variety of organic amendments exist, there is limited empirical data on their comparative effectiveness and application strategies in transplant production. This two-year greenhouse study evaluated the effects of selected organic amendments on the growth, root system architecture, and nutritional composition of pepper (Capsicum annuum) transplants grown in 25-cell trays. The experiment was arranged in a randomized complete block design with four replications. Treatments included three dry organic fertilizers bone meal (3-15-0), blood meal (12-0-0), and feather meal (12-0-0) mixed at recommended label rates with growing media at the time of seeding. Additionally, treatments included a liquid fish emulsion (5-1-1 AquaPower™), compost amendment, conventional synthetic (15-5-15 Peters Excel®), and a no-fertilizer control. Data was collected on plant height, stem diameter, plant biomass, tissue nutrient content, and chlorophyll content of leaves. Root and shoot biomass were also collected followed by analysis of root architecture using WinRhizoTM software. Synthetic fertilizer treatment resulted in the highest plant biomass and height, followed closely by blood meal, with no statistically significant difference between the two in either year. Root surface area and volume were greatest in plants treated with feather meal, followed by those receiving blood meal. Weekly EC and pH data collected on the growth medium leachate samples showed that there was a strong negative correlation between them that is when EC was high, pH was low across the treatments, with compost treatment having the highest EC and pH overall. These findings underscore the influence of organic fertilizer source on pepper transplant growth and nutrient status, contributing to improved organic transplant production practices and enhanced transplant quality for vegetable growers.
Speakers
SC

Smriti Chaudhary

Iowa State University
Co-authors
AN

Ajay Nair

Iowa State University of Science and Technology
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

ORGH 1 - The tomato organic management and improvement (TOMI) project: helping tomato growers better manage foliar diseases
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Tomato (Solanum lycopersicum) growers report that foliar diseases are their biggest production challenge. The tomato organic management and improvement (TOMI) project was launched in 2014 with support from the NIFA-OREI program to address this challenge. We have applied a transdisciplinary approach, integrating studies aimed at 1) increasing biocontrol efficacy, 2) understanding mechanisms controlling induced systemic resistance (ISR), and 3) developing new varieties using a participatory breeding approach. In our biocontrol studies, we learned that combining products with different modes of action was not effective, however, if applied early and often, some biocontrol agents can reduce disease across diverse locations and years. Potting media and composts containing residues with high carbon to nitrogen ratios can increase survival and efficacy of a soil-applied biocontrol agent. In our ISR studies, we learned that not all tomato genotypes are responsive to this form of disease control. The most responsive are wild relatives (Solanum pimpinellifolium), which release distinct compounds from their roots to signal and support soil microbes with biocontrol capabilities. ISR responsiveness is associated with upregulation of brassinosteroid and phenylpropanoid pathways, and grafting appears to promote ISR activity. Identifying genetic markers will improve selection for this trait. Finally in our breeding program, we learned that engaging growers in the selection process aids in the development of new varieties with the most desirable set of traits. We made significant gains in advancing populations and some advanced lines are being considered for release by seed companies, though resistance in many populations is not effective in all ecoregions. Regionally-focused breeding programs are likely to be more effective in developing varieties best adapted to local environmental conditions and disease complexes. We are continuing to work closely with growers to advance these efforts. More information about our project and resources for growers can be found on our website: https://eorganic.info/tomi.
Speakers
LH

Lori Hoagland

Purdue Univ - West Lafayette, IN
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 2
  • Subject Organic
  • Poster # csv
  • Funding Source NIFA-OREI
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

1:00pm CDT

TECH 1 - A Smartfarm System in Idle Facilities
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
The number of empty houses in South Korea is expected to surge due to rapid population decline. The problem is being overcame through remodeling and leasing in other countries. However, those businesses are not productive and sustainable. Considering climate change and the decline in the agricultural population, we would suggest a project to use empty houses for agricultural purposes. Smartfarm technology allows us to grow crops anywhere, thus farming in the apartments is possible. In particular, since most apartments in South Korea are complexed, large-scale cultivation is possible. We expect economic effect (sales revenue, local food market, job creation, real estate value, distribution process), social effect (connection between cities and rural areas, food security, local communities) and environmental effect (carbon reduction, sustainability, urban green space) from this project. Taking advantage of the characteristics, we believe that it is necessary to proactively conduct a study on a smart value chain system that connects cultivation, storage, retail, and restaurants.
Speakers
DS

Dong Sub Kim

Kongju national university
Co-authors
CS

Chan Saem Gil

Kongju national university
NA
HK

Hye Kyoung Jahng

Chungbuk National University
NA
HC

Hyo-Gil Choi

Kongju national university
NA
SK

Seok - Kyu Jung

Kongju national university
NA
SH

Seong Heo

Kongju national university
NA
SK

Si-Yong Kang

Kongju national university
NA
TH

Tae Hwa Kim

Kongju national university
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Technology Applications in Horticulture 1
  • Subject Technology Applications in Horticulture
  • Poster # csv
  • Funding Source Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry(IPET) and Korea Smart Farm R&D Foundation(KosFarm) through Smart Farm Innovation Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs(MAFRA) and Ministry of Science and ICT(MSIT), Rural Development Administration(RDA)(RS-2024-00400011)

1:00pm CDT

TECH 1 - An AI-enhanced Soft Robotic System for Selective Strawberry Harvesting
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Strawberry harvesting is a labor-intensive task that requires careful, selective picking to avoid damaging delicate fruit. To address labor shortages and enhance harvesting efficiency, we propose an AI-enhanced soft robotic system capable of autonomous and selective strawberry harvesting. Specifically, the system incorporates advanced computer vision algorithms, leveraging the Grounding DINO model, to detect and localize ripe strawberries with high accuracy. A compliant soft robotic gripper, guided by real-time perception, then gently harvests only the target fruits, minimizing potential damage to adjacent berries and plants. Experimental results demonstrate that the system achieves a ripe strawberry detection accuracy of 88% and a harvesting success rate of 66.67%. This integrated approach offers a scalable, precise, and labor-efficient solution for modern strawberry production.
Speakers
DC

Dong Chen

Assistant Professor, Mississippi State University
Co-authors
CO

Cheng Ouyang

Mississippi State University
NA
JL

Jiajia Li

Michigan State University
NA
MU

Moeen Ul Islam

Mississippi State University
NA
QZ

Qianwen Zhang

Truck Crops Branch Experiment Station
XQ

Xinda Qi

Michigan State University
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TECH 1 - Effects of Non-Thermal Plasma on Seed Germination Metrics of Borage
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Seed germination may be affected by various abiotic and biotic stressors, resulting in significantly reduced crop yields and resource use efficiency, thus posing challenges to food production for the growing global population. Therefore, several studies have focused on employing diverse mechanical and chemical treatments to improve seed germination. Non-thermal plasma (NTP) is an emerging technology for enhancing seed germination and plant growth. This study evaluated the effects of NTP on the germination properties of borage (Borago officinalis L.) seeds. Plasma was generated using a custom-made surface-barrier-discharge (SBD) device, operating at a frequency of 5 kHz and voltage of 1.3 kVpp. Seeds were exposed to plasma for 0.5, 1, and 2 minutes, whereas untreated seeds served as the control. Each treatment was replicated three times, with each replication divided into two subunits, containing 25 seeds per experimental unit. Germination was monitored under controlled conditions (20 °C temperature, 16 hours photoperiod, 200 µmol/m2/s PAR radiation provided by fluorescent lights and 74% relative humidity) in a PGR-15 Conviron plant growth chamber for 10 days. Germination was assessed daily, and key germination parameters were analyzed, including final germination percentage (GP), mean germination time (MGT), mean germination rate (MGR), coefficient of variation of germination time (CVt), germination index (GI), and time to 50% germination (t50). Plasma treatment for 0.5 and 1 minute significantly (p < 0.05) increased germination percentage (GP) (52 ± 5.37% and 50.67 ± 2.46%, respectively) compared to the control (34.67 ± 3.96%). MGT decreased in all plasma-treated groups, with the shortest MGT (3.94 ± 0.13 days) observed for 0.5 minutes compared to 4.91 ± 0.29 days for the control, suggesting faster germination. Plasma treatment significantly enhanced MGR, with shorter exposure time leading to accelerated germination. All plasma-treated groups reached 50% germination faster than the control, suggesting improved seed vigor. The GI of plasma-treated seeds was consistently higher than the control, indicating greater germination uniformity. These findings highlight the potential of non-thermal plasma treatment to enhance key factors for improved yield and crop resource use efficiency.
Speakers
SS

Sanchita Saha

The Pennsylvania State University
Co-authors
CA

Calixto Alvarado

The Pennsylvania State University
NA
CM

Colby Mathews

The Pennsylvania State University
NA
FD

Francesco Di Gioia

Pennsylvania State University
JB

Jada Bernitt

The Pennsylvania State University
NA
SK

Sean knecht

The Pennsylvania State University
NA
SB

Sven Bilen

The Pennsylvania State University
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Technology Applications in Horticulture 1
  • Subject Technology Applications in Horticulture
  • Poster # csv
  • Funding Source The research was funded by the Pennsylvania Department of Agriculture Specialty Crop Block Grants No. C940001529 “Developing Plasma Agriculture Solutions to Improve Vegetable Yield and Quality” and was supported by the Strategic Networks and Initiatives Program (SNIP) “Developing the Penn State Interdisciplinary Initiative on Plasma Agriculture”, funded by the Penn State College of Agricultural Sciences. FD's contribution was funded by the USDA National Institute of Food and Agriculture and Hatch Appropriations under Project #PEN05002, Accession #7007517.

1:00pm CDT

TECH 1 - Establishing a Small-Scale Agrivoltaic Research and Demonstration Plot on the Navajo Nation: Evaluating PV Panel Shading Effects on Radish Growth
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Agrivoltaic systems, which integrate photovoltaic (PV) panels with agricultural production, offer potential benefits for sustainable land use but remain underexplored in many regions. A needs assessment survey conducted among stakeholders on the Navajo Nation identified both an interest in agrivoltaics and a lack of understanding regarding its implementation and impacts. In response, a pilot study was established to evaluate the effects of PV panel shading on microclimatic conditions and radish (Raphanus sativus) productivity in a controlled small-scale agrivoltaic system. This study aimed to generate preliminary data to inform future agrivoltaic research and applications. The experiment utilized open-bottomed grow boxes filled with a 2:1 mixture of commercially available peat-perlite growing medium and organic mushroom compost, placed over field soil. Radish seeds were sown under four different PV panel treatments and a full-sunlight control, with environmental parameters—including air temperature and light intensity—monitored throughout a 46-day growth period. At harvest, crop yield and soil characteristics, including nutrient content, electrical conductivity, pH, and heavy metal concentrations, were analyzed. Morphological parameters such as total root biomass and the proportion of marketable roots (≥16 mm in diameter) were also assessed. Results indicated that PV panel shading reduced radish productivity, with shaded treatments exhibiting lower root biomass and yield compared to the full-sunlight control. These findings suggest that shading effects from PV panels may negatively impact radish growth under fall seasonal conditions. This study provides critical baseline data for optimizing agrivoltaic system designs based on crop selection and environmental conditions, contributing to broader research on sustainable agricultural practices in arid and semi-arid regions.
Speakers
EM

Emiliano McLane

New Mexico State University
Emiliano McLane (Newe) is from the Tosawihi clan of the Te-Moak Tribe of Western Shoshone and was primarily raised on the South Fork Indian Reservation near Jiggs, Nevada. He is also a descendant of the Pomo, Wailaki, and Nomlaki tribes and spent part of his upbringing on his father’s... Read More →
Co-authors
CV

Ciro Velasco-Cruz

New Mexico State University
NA
DE

Don Edgar

New Mexico State University
NA
KL

Kevin Lombard

New Mexico State University
Kevin Lombard is a Professor of Horticulture and Research Director of the New Mexico State University Agricultural Science Center at Farmington, a 250 acre research farm uniquely located on the Navajo Nation. His research encompasses specialty crop evaluations including grapes, fruit... Read More →
OL

Olga Lavrova

New Mexico State University
NA
SN

Shannon Norris Parish

New Mexico State University
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Technology Applications in Horticulture 1

1:00pm CDT

TECH 1 - Large Format Aeroponics System (LFAS) to Study Differential Drought Response in Geneva Rootstocks
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Major U.S. apple producing regions include Washington, Michigan, and New York for an aggregate of 10 billion pounds of apples/year. While most apple orchards in the Western region feature irrigation systems, large portions of acreage in the Midwest and Eastern regions rely on rainfall and are sensitive to lengthy dry periods during the growing season. These dry periods have been shown to cause fruit drop and drastically reduce fruit size. Apple rootstocks represent the interface between soil and scion and are a critical component of water relations in orchards. Understanding rootstock response to drought is a key component of securing apple production in vulnerable regions. In this study, we utilized a large format aeroponics system (LFAS) with integrated sensors for light, root moisture, and caliper to conduct an exploratory experiment on the utility of the LFAS to study rootstock behavior during drought in real time. The LFAS contained 12 trees of 6 rootstock genotypes grafted with ‘Honeycrisp’. Each tree was suspended into the LFAS with a collar and the roots sprayed with a pH-balanced nutrient solution at intervals of 30” on/1’30” off. Trees were grown from June-November in a heated greenhouse (24oC) with supplemental lighting. We selected three rootstocks (G.890, G.935, G.969) to monitor before, during, and after drought using LiCor-600 porometer readings. Prior to treatment, we selected four newly expanded leaves at the top of the tree and two mature leaves in the mid-section, labelling them N1-N4 (newer leaves) and O1-O2 (older leaves). Baseline porometer readings were collected at 4:30 AM and then at 12:00 PM under full sun and supplemental light. Subsequently, solution misting was shut down from 12:00 PM to 4:00 PM on November 11th, 2022. In aeroponics, solution film provided by misting and reserve moisture in the roots are all that is available for evapotranspiration. As a result, the drought treatment was almost instant. Roots appeared dry and both apical sections of the trees and mature leaves wilted. After 2 weeks, leaf damage was recorded. Post-drought, N1-N4 and O1-O2 leaves were measured again with the porometer, as well as two “Drought Damaged” leaves (DD1-DD2). Statistical analysis indicated significant Drought*Genotype effects (p < .0001). Dendrometer readings showed the differential response to drought of each rootstock in real time. This experiment demonstrated that aeroponics is a viable method for assessing drought tolerance of rootstock-scion combinations, and showed drought tolerance of the rootstocks was G.935 > G.890 > G.969.
Speakers
DU

Davis Upchurch

Cornell University
Co-authors
GF

Gennaro Fazio

USDA-ARS, Cornell
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TECH 1 - Reliability Assessment of Agricultural Sensors Using Algae Coverage Analysis: Implications for Data Quality and Crop Yield Monitoring
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Modern agricultural systems, notably hydroponics, utilize digital technologies and precision farming techniques for automated, sensor-driven cultivation. These systems apply real-time environmental monitoring to enhance plant growth parameters and operational efficiency. Nevertheless, sensor inaccuracies can undermine data integrity due to malfunctions or environmental influences. Thus, this is required to obtain comprehensive environmental analysis and precise data management protocols to maintain system integrity. This study assessed the sensors' reliability by evaluating algal coverage metrics in hydroponic tomato cultivation. A total of one hundred seventeen sensors designed to measure pH, temperature, humidity, and electrical conductivity (EC) were deployed within a greenhouse environment. Following a duration of three months, only 39 sensors were classified into two separate categories: 22 demonstrating considerable algal coverage (≥90%) and 17 exhibiting minimal coverage (~10%). Sensors characterized by high algal coverage recorded substantially increased substrate humidity (85.6% vs. 41.9%) and EC values (774 µS/cm vs. 331 µS/cm) in contrast to their counterparts with lowered algae coverage. However, tomato yields did not significantly change between the two categories, indicating that the plants accommodate the changing environmental conditions. These results suggest that algal coverage may serve as an indirect metric for evaluating localized environmental parameters—particularly humidity and EC levels—and may also hold potential value in assessing the reliability of sensor data.
Speakers
avatar for Saksonita Khoeurn

Saksonita Khoeurn

BigDataLabs Co., Ltd.
NA
Co-authors
DS

Dong Sub Kim

Kongju national university
EL

Eunji Lee

Kongju national university
NA
HK

Hye Kyoung Jahng

Chungbuk National University
NA
JJ

Jaehyuk Jeon

eney Co., Ltd.
NA
JY

Ji Yun Yun

Kongju national university
NA
NP

Noyeon Park

Chungbuk National University
NA
SJ

Seungback Jung

WHYBIZ Corp.
NA
WC

Wan-Sup Cho

Chungbuk National University
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Technology Applications in Horticulture 1
  • Subject Technology Applications in Horticulture
  • Poster # csv
  • Funding Source Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry(IPET) and Korea Smart Farm R&D Foundation(KosFarm) through Smart Farm Innovation Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs(MAFRA) and Ministry of Science and ICT(MSIT), Rural Development Administration(RDA)(RS-2024-00400011)

1:00pm CDT

TECH 1 - UAV Remote Sensing for Western Mayhaw Flower Intensity Assessment
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
UAV Remote Sensing for Western Mayhaw Flower Intensity Assessment Presenting Author: Austin Fruge’ Co-Authors: Dr. Cengiz Koparan, Dr. Donald M Johnson, Dr. Amanda McWhirt Abstract. Western Mayhaw (Crataegus opaca) is an emerging economically important fruit in the genus Crataegus due to increased consumption, expanded marketing, and improved cultivars. Further research is needed to expand technology-driven management strategies and investigate its potential as an economical crop for rural and urban landowners in the Southeastern United States. The current methodology for estimating flowering intensity assessment in Western mayhaws is performed with visual observation in the field. However, this methodology is time-consuming, labor-intensive, and subjective. Given the need for a precise methodology for flowering intensity monitoring in Western mayhaws, we developed an open-source image-based phenotyping workflow from Unmanned Aerial Vehicle (UAV) captured images. A subset of Western mayhaw selections were evaluated for blooming intensity during the spring of 2025 in a private orchard near El Dorado, Arkansas. RGB images of Western mayhaw trees during the early flowering stage were collected using a DJI Mavic 3 Enterprise UAV mounted with an RGB digital camera. Each image was processed using an open-source image processing software to estimate the number of flowers. To evaluate the accuracy of this method, the flowering intensity was evaluated through visual flower counting and a visual scale, and compared to image-based flower estimation. Flowering intensity estimated with image segmentation showed a strong correlation with visual flower counting (r= 0.858, p < 0.001), indicating that an increase in visual flower count can be explained with segmented pixel count for any random image. Flower estimation with image segmentation is accurate and provides a standard method, however, it could be time-consuming due to the large image dataset. A semi-automated or fully automated image processing workflow could be developed to increase the efficiency of image processing.
Speakers
AF

Austin Fruge

University of Arkansas
Co-authors
AM

Amanda Mcwhirt

University of Arkansas
CK

Cengiz Koparan

University of Arkansas
NA
DJ

Donald Johnson

University of Arkansas
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 1 - Assessment of the Effects of Modified Polyamine Metabolism on Phenotypic Plasticity and Structure-Function Coordination of Tomato Plants Under Deficient Irrigation
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
The development of genotypes resilient to abiotic stresses like drought has a pivotal role over the impacts of climate extremes on global food security. Recent studies suggest that modulating the activity of polyamine oxidases (PAOs) in crop species could enhance stress tolerance. Given the limited data on how altered PAOs expression (either overexpression or silencing) affects physiological and anatomical traits that may contribute to plant resilience, the aim of the present study is to investigate phenotypic variability - focusing on leaf structure and function - in tomato genotypes with modified polyamine metabolism under deficient irrigation. Three tomato genotypes were cultivated in a greenhouse, one reference (cv. Moneymaker) and two transgenic lines of the reference genotype with silenced expression of the genes encoding the homologous enzymes SlPAO3 and SlPAO4 of PAO. Two irrigation regimes were applied: Control (100% field capacity, FC) and Drought (20% FC). Following the development of new leaves under drought conditions (long-term acclimation), anatomical features of the leaves and petioles, as well as gas exchange parameters, were assessed. Results showed that, regardless of genotype, water deficit led to a reduction in leaf area, reflecting a decrease in transpiring surface, an adaptation of leaf function under limited water availability. The slpao3 plants, regardless of the irrigation regime, exhibited a smaller total vascular tissue area per petiole cross-section compared to both the reference genotype and the slpao4 plants. However, during acclimation to drought stress, an increase in vascular tissue area was observed only in the slpao3 plants. These findings align with previous studies highlighting the involvement of polyamines in xylem differentiation processes. Long-term acclimation resulted in a reduction in xylem vessel size and an increase in vessel density, irrespective of genotype, indicating an adjustment of hydraulic traits to meet the water demands of acclimated leaves. Petiole vascular characteristics, leaf area, and gas exchange parameters, independent of genotype and treatment, showed strong correlations among them. These results confirm a coordination presence between hydraulic function and photosynthetic response. Further research is needed to clarify how this coordination influences water transport and use efficiency, and to explore whether modulation of PAOs activity could serve as an effective strategy to enhance plant resilience to environmental stresses.
Speakers
IK

Ioannis Karapanos

University of Florida / North Florida Research and Education Center
Co-authors
AB

Aliki Boura

Crop Science Athens Attica Greece, Agricultural University of Athens
AK

Andriana Karachaliou

Crop Science Athens Attica Greece, Agricultural University of Athens
DN

Dimosthenis Nikolopoulos

Crop Science Athens Attica Greece, Agricultural University of Athens
EK

Evangelos Kyrkoulis

Crop Science Athens Attica Greece, Agricultural University of Athens
GL

Georgios Liakopoulos

Crop Science Athens Attica Greece, Agricultural University of Athens
PB

Panagiota Bresta

Crop Science Athens Attica Greece, Agricultural University of Athens
PT

Paraskevi Tavladoraki

Department of Science, University Roma Tre
SC

Styliani Chorianopoulou

Crop Science Athens Attica Greece, Agricultural University of Athens
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 1 - Correlation Analysis of Short-day Onion Bulb Characteristics as Indicators of Firmness to Reduce Bulb Bruising from Mechanical Harvesting
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Short-day onions are grown for fresh market in the southern region of the US, and they require around 10 to 12 hours of photoperiod or 12 to 14 hours of nighttime to thrive. These onions tend to produce fewer, thinner layers and have higher moisture contents, making them susceptible to bruising. When short-day onions are harvested by machines, a small percentage of them are damaged and the rest experience bruising during the harvesting process. This leads to a focus on bulb firmness and how it can affect the reduction of bruise damage. The current study seeks to identify bulb characteristics that affect bulb firmness and their relation to the overall bruise damage in short-day onions. For this, early and main season maturity varieties were used and several bulb characteristics including bulb weight (gm), height (cm), width (cm), number of rings, firmness, growing point (single versus multiple centers), and total soluble solids (°Brix) were measured. Correlation among bulb traits was analyzed using correlation in R corrr package. In the early season, there was correlation between bulb weight and bulb width (r=0.920343), firmness and single center (r=0.504853), and single center and ring number (r=0.609349). There was no significant correlation between bulb firmness and the remaining bulb traits. A negative correlation was observed between firmness and moisture content (r=- 0.412323) and firmness and ring number (r=- 0.5084) in the main maturity season trial, but not enough to significantly impact the bulb firmness. The results suggested that in early maturity bulbs, a single center directly correlated with firmer bulbs, but in the main maturity season, firmness had a negative correlation with moisture content and ring number. Results will be validated with the second season trial during the 2024-2025 season.
Speakers Co-authors
CC

Christopher Cramer

New Mexico State Unimersity
NA
LK

Lakhvir Kaur

Texas A
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 1

1:00pm CDT

VCM 1 - Evaluating diverse sweetpotato breeding lines in western Washington
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Orange-fleshed sweetpotato (Ipomoea batatas) cultivars with moist, sweet texture are the most popular type of sweetpotatoes grown in the United States, while elsewhere in the world, white, yellow, and purple-fleshed types with firm, dry texture are more popular. There is a market opportunity in the United States for diverse sweetpotato cultivars, however, their yield tends to be low as they have not received the same breeding attention as orange-fleshed cultivars. In this experiment, six advanced breeding lines from the USDA, representing a diversity of flesh colors, textures, sweetness, and firmness, were compared to cvs. Covington (standard) and Cascade (new release). The experiment was carried out in a certified organic field in Mount Vernon, WA in 2024 and is being repeated in 2025. Each plot included 15 plants with 4 replicate blocks. In 2024, sweetpotatoes were planted on 4 June in raised beds covered with black polyethylene mulch and with drip irrigation. Harvest was by hand on 7 Oct., roots were sorted by size, and damage by wireworms (Agriotes lineatus, A. obscurus, Limonius canus), the most significant insect pest impacting sweetpotatoes in this region, was assessed using the WDS severity index ( 0 = no scars, 1 = 1-5 scars, 2 = 6-10 scars, 4 = >10 scars). Without accounting for wireworm damage, yield (US No. 1 and US No. 2) of ‘Covington’ (orange, soft flesh) was highest (18.2 t/ha) followed by USDA-22-009 (13.8 t/ha, yellow flesh), and ‘Cascade’ (white, firm flesh) had low yield (6.7 t/ha) followed by purple-fleshed breeding lines USDA-17-090 (4.1 t/ha) and USDA-16-051 (3.1 t/ha). Orange-fleshed breeding lines USDA-16-031 (3.8 t/ha) and USDA-19-036 (1.7 t/ha) and yellow-fleshed USDA-23-039 (0.5 t/ha) all had low yield. ‘Cascade’ was the most wireworm resistant (WDS severity index 1.61) followed by USDA-16-051 (2.33), while ‘Covington’ and USDA-22-009 were the most susceptible (3.67 and 3.54, respectively). The breeding lines USDA-16-031, USDA-17-090, USDA-19-036, and USDA-23-039 had similar ratings, ranging from 2.83 to 3.39. While yields of ‘Cascade’ (white, firm flesh) and USDA-16-051 (purple flesh) were less than ‘Covington’, farmers may be interested in growing these niche cultivars for their wireworm resistance and to cater to consumer interests.
Speakers
JW

Jordan White

Washington State University
Co-authors
CM

Carol Miles

Washington State University
LS

Laura Schulz

Washington State University
PW

Phillip Wadl

USDA, ARS, U.S. Vegetable Laboratory
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 1 - Garlic Cultivar Trial and Allicin Content Analysis in Oklahoma
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Garlic (Allium sativum) is a cool season and flavorful vegetable crop that has a long history in culinary, horticultural, medicinal uses. Oklahoma is a state characterized by diverse weather and soil types, but there is an economic opportunity for garlic production with optimal planting techniques and planting recommended cultivars. The number of farmers producing garlic increased from 28 to 44 between 2017 and 2022. This trial was conducted at the Cimarron Valley Research Station with Oklahoma State University in Perkins, Oklahoma and evaluated ten cultivars grown using drip tape irrigation, no mulch, and a no-spray approach to weed and pest control. Total graded marketable yield and allicin content were recorded during the 2023-2024 and 2024-2025 growing season for softneck and hardneck cultivars including: ‘California Early’, ‘Chesnock Red’, ‘Duganski’, ‘German Extra Hardy’, ‘German White Stiffneck’, ‘Inchellium Red’, ‘Music’, ‘Purple Glazer’, ‘Romanian Red’, and ’Sicilian Artichoke.’ In 2024, the top performing cultivars were ‘German Extra Hardy’ and ‘Inchellium Red’. These were significantly higher yields that ‘Duganski’. No other statistical differences were observed. Their respective marketable yields were 2,704 and 2,538 pounds per acre. In addition, we recorded allicin content for each cultivar using a spectrophometric assay. Allicin is one of the most important thiosulfates in garlic and its presence adds market value for those interested in the nutritional and health benefits of garlic. We observed statistically significant differences among the cultivar entries. ‘California Early’ and ‘Duganski’ had a significantly higher allicin content at .66 grams and .59 grams per 100 g sample than ‘German White Stiffneck’ at .49 grams per 100 g sample. Additional research of allicin content among different cultivars of garlic is needed to guide consumers and healthcare professionals in selecting the best cultivar for fresh market consumption.
Speakers
VW

Victor Ware

Oklahoma State University
NA
Co-authors
TM

Tyler Mason

Oklahoma State University
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 1

1:00pm CDT

VCM 1 - Organic pest management of broccoli using mesotunnels in the Midwest
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Broccoli, scientifically known as Brassica oleracea var. italica, was first cultivated in the 17th century. Based on 2022 USDA Ag census, in the United States, broccoli is grown on 96,600 acreage. Broccoli is damaged by more than 25 insect pests. Some pests are region/place specific and some are common across the world like aphids, mites, etc., Pests like imported cabbage worms, cabbage loopers, and diamondback moths have a destructive effect on the crop in different growth stages of the crop. Organic pesticides to manage these pests have mixed results and account for increased labor and costs. Mesotunnels could serve as a viable pest management approach within organic production systems. “Meso” means “middle,” so mesotunnels are medium-size tunnels – taller than low tunnels and shorter than high tunnels. A breathable nylonmesh fabric (brand names include ProtekNet and ExcludeNet) that resembles window screen are placed over the mesotunnel. The fabric acts as a barrier for insect pests and prevents their entry and disease-carrying pathogens. These nylon nets are breathable, durable, and light permissible which can also protect from extreme weather conditions like hail damage. This study includes the following three treatments: 1) Mesotunnel with proteknet, 2) Organic insecticides, and 3) Control (no mesotunnel or organic insecticides). Mesotunnel treatment resulted in less pest damage (No.of holes/plant) with a mean 14 when compared to organic insecticides which had mean damage of 22. A significant difference was observed in pest damage between the treatments. Regarding marketable yield, the highest was observed in the Mesotunnel treatment. However, no significant difference was found between the treatments. This study aims to identify the most effective method of pest management in broccoli production while minimizing the environmental impact through reduced reliance on pesticides. Keywords: mesotunnels, proteknet, excludenet, organic pesticides
Speakers
AT

Akshaya Thinakaran

Iowa State University
Co-authors
AN

Ajay Nair

Iowa State University
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 1 - Screening for Cucumber with Disease and Pest Resistance and Consumer-Defined Visual Appeal in the Virgin Islands
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Matching breeding target with consumer preference is the goal of many crop production methods. But this is normally a difficult challenge when the selected disease- and pest-resistant materials do not meet the consumers’ product preference. In this report, we highlight the efforts we are making to deliver cucumber lines with consumer-preferred visual traits and that have combined resistance to melonworm, leaf miner, aphids, and Downy mildew. After a series screening of 100 lines from the USDA, 36 showed at least 40% resistance to leafminer and melonworm in 2022 and 2023. Of these, we have evaluated 16 against lines with varying degrees of resistance and six susceptible lines against three commercial varieties commonly grown in the Virgin Islands. All the 25 cucumber lines were exposed to open natural infestation by leaf miner, melonworm, aphids and downy mildew in the open field and scored for resistance based on visual ratings designed for each infestation. We used a mathematical model that considered multiyear and multilocation performances to rank the lines for combined resistances. Unfortunately, the top three resistant lines were not among the top five visually appealing fruit types on a consumer preference scale based on a survey of 245 respondents. We report on the prospects and anticipated timeline for the development of cucumber plants with the preferred fruit type and disease and pest resistance traits. Our study has the potential to deliver cucumber varieties that are highly adapted to the Caribbean abiotic and biotic stresses and that have significantly reduced pesticide footprints. Key words: Consumer preference, cucumber, pest resistance, screening, trait
Speakers
JE

Jessica Ewer

University of the Virgin Islands
Co-authors
AD

Amy Dreves

University of the Virgin Islands
NA
HA

Henry Awika

University of the Virgin Islands
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 1

1:00pm CDT

VCM 1 - Stop, Dry, Lignify: Simulated Pre-harvest Water Deprivation Increases Continuity of Lignified Periderm Tissue and Reduces Postharvest Weight Loss in Sweetpotato Storage Roots
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
A greenhouse culture system simulated in-ground curing by imposing pre-harvest water deprivation treatments in sweetpotato. Virus-tested sweetpotato cv ‘Beauregard’ cuttings were planted at the following staggered times relative to the control (0d): 10 days (10d) and five days (5d) earlier, respectively. Water deprivation treatments were applied 10d and 5d pre-harvest. The first study terminated experiments when the control plants attained 30 days of growth. Storage root sections 1-2 mm thick were obtained from the broadest part of the storage root, stained with phloroglucinol, followed by image capture with a microscope. Digital image analysis was performed by measuring the area of the continuous lignified zone (CLZ) below the phellogen layer and calculating % CLZ after measuring the total area of the cross-section. Simulated WD at 10d and 5d was associated with a six- and three-fold increase in % CLZ, respectively. None of the control SRs showed 100% CLZ, while 50% of SRs subjected to 10d WD showed an intact ring of lignified tissue below the phellogen layer. Storage roots from control plants consistently showed breaks in the phellem tissue relative to SRs subjected to WD treatments. In the second study, plants were grown for up to 50 days, and harvested SRs were kept in ambient conditions for weight loss measurements. Water deprivation was associated with reduced weight loss in SRs relative to SRs grown in well-watered conditions. These findings can lead to follow-up work to validate increased skin durability with pre-harvest, in-ground curing in production areas where postharvest curing facilities aren’t available.
Speakers
avatar for Arthur Villordon

Arthur Villordon

Professor, Louisiana State University AgCenter
No roots left behind! The sweetpotato's root system architecture integrates internal, environmental, and management determinants of storage root yield, quality, and shape attributes. Root architectural adaptations to biotic and abiotic stress variables provide insights about traits... Read More →
Co-authors
MA

Mae Ann Bravo

Louisiana State University
MB

Marissa Barbosa

Lousiana State University
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 1

1:00pm CDT

VCM 1 - Sweetpotato Cutting Length Influence on Production in the USVI
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Sweetpotato (Ipomoea batatas) is a tuberous root crop and an important agricultural staple in the U.S. Virgin Islands. Due to its year-round production potential, drought tolerance, adaptability to calcareous soils, and cultural significance, a more efficient land-use and cropping system is needed. The objective of this study was to investigate the impact of sweetpotato cutting length on yield per linear foot on five cultivars, ‘Bonita’, ‘Bellevue’, ‘GA-11’, ‘Murasaki’ and ‘Sakura-40’. Actively growing vines from five cultivars were propagated using 6–8-inch terminal cuttings and 3-foot cuttings. An 8-inch drip irrigation system was used for both treatments. After 130 days, harvest data were collected on number of tuberous roots, weight, and marketable yield. Results showed that Bonita, Bellevue, and Murasaki produced higher yields (kg/ft) when planted with 6–8-inch cuttings, whereas ‘GA-11’ and ‘Sakura-40’ yielded more with 3-foot cuttings. Similarly, ‘Sakura-40’, ‘Bonita’, and ‘Bellevue’ had greater numbers of marketable and total roots per foot with 6–8-inch cuttings, while ‘Murasaki’ and ‘GA-11’ performed better in both categories with 3-foot cuttings. These findings indicate that optimal cutting length is variety-specific, with certain cultivars favoring shorter cuttings for higher yield and root number, while others benefit from longer cuttings. This research was funded by a USDA Mutistate Hatch Grant.
Speakers
IC

Imhotep Charles

University of te Virgin Islands
NA
Co-authors
SJ

Samuel Joseph

University of the Virgins Islands
NA
TZ

Thomas Zimmerman

University of the Virgin Islands School of Agriculture
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 1

1:00pm CDT

VCM 1 - Tradeoffs of Rowcover Management Approach on Winter Spinach Crop in Unheated High Tunnels in Cold Climates
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
In the northeastern US, cold-tolerant crops such as spinach are an important source of winter income for high tunnel growers. Supplemental rowcovers are used on crops inside unheated high tunnels to expose plants to optimal temperature ranges for longer periods of each day and to provide greater protection from injurious low temperatures. Rowcover management impacts temperature, light availability, humidity, pest populations, crop yield, and labor requiremenst. Some growers actively manage rowcovers, removing them daily to optimize light exposure and then reapplying them in the evening and on cloudy or very cold days. This is thought to provide a yield benefit due to increased crop light availability, but it is labor intensive and experienced growers often make decisions about removal based on a combination of current conditions and forecasted weather. Therefore, some growers opt for a “set it and forget it” approach, where layers are applied once and then only removed as needed, rather than daily. Here we describe the tradeoffs of “active” rowcover management, where rowcovers are removed during the day and replaced nightly, compared to “passive” management, where rowcover is removed only for harvest and regular maintenance. We conducted a replicated trial in two experimental years in an unheated high tunnel to investigate the effects of rowcover management on winter spinach yield, pest abundance, and abiotic environmental factors including growing degree hour accumulation. We also compared our observations with those from commercial tunnels managed by three experienced growers in the northeast. In both experimental years, growing-degree hour accumulation and the cumulative amount of time below freezing did not differ significantly between active and passive management. However, active rowcover management did result in greater light accumulation, lower relative humidity, and increased spinach yields in one experimental year compared with passive management.
Speakers
avatar for Becky Sideman

Becky Sideman

ASHS Board Member-At-Large
Co-authors
LH

Lilly Hartman

University of New Hampshire
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 1
  • Subject Vegetable Crops Management
  • Poster # csv
  • Funding Source Funded by NIFA-SCRI 2021-07737 and the NH Agricultural Experiment Station
  • Funding Option SCRI funded all or part of the research associated with this abstract

1:00pm CDT

VCM 1 - Using washed-up semi-decomposed seagrass in sweet potato cultivation
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Seagrass that washes ashore is an abundant, traditional amendment for crop production on Yap Islands, yet its potential for modern sweetpotato (Ipomoea batatas) cultivation remains largely unexplored. Traditionally, semi-decomposed seagrass is incorporated to create raised beds where sweetpotato is grown for several years. While compost and manure can be effective amendments to improve the conditions of the highly weathered, acidic volcanic soils of the Yap Islands, they are costly, labor intensive, and in short supply. By utilizing an abundant, free resource, this project seeks to improve crop yields and extend the productive life of agricultural lands. This study includes two field experiments that compare semi-decomposed seagrass with conventional commercial fertilizer for two sweetpotato cultivars in two production systems, raised-beds and grow-bags. Experiment 1 utilizes raised beds established on three farm sites with an improved sweetpotato cultivar, where treatments include medium and high application rates (by weight, approximately 40 and 60 kg N ha-1, respectively) of semi-decomposed seagrass and a control treatment using commercial fertilizer (10N-20P-20K) at N rate equivalent to the medium seagrass application. Each treatment is randomized in a complete block design, with six replicates per treatment. Experiment 2 is a split-plot design and compares a traditional vining and a modern sweetpotato cultivar (main plot treatment) in container cultivation using 10-gallon grow bags filled with a soil mix amended with seagrass at medium and high ratios compared to a commercial fertilizer control at N rate equivalent to the medium seagrass application (split-plot treatment). For both experiments, the seagrass is collected, washed, and piled to reduce salinity to acceptable thresholds, with leachate EC measured weekly. Seagrass is then shredded and piled to accelerate decomposition. Analysis results of semi-decomposed seaweed are 15% dry weight with 1.8% N on average, and application rates are 21 g and 32 g per sq foot of soil for N application rates of 40 and 60 kg.ha-1, respectively. Community buy-in and trust building are being achieved through collaboration with island-based agricultural educators and meetings with traditional leaders. This research has the potential to enhance sweet potato production on the Yap Islands, improve resource management, and provide an affordable soil amendment option for local farmers. The project promotes environmental stewardship and contributes to cleaner shorelines and sustainable agricultural practices. The findings will offer critical insights for local agricultural educators and policymakers in optimizing land use and advancing low-cost, high-yield cultivation methods in resource-limited settings.
Speakers
CM

Carol Miles

Washington State University
Co-authors
SY

Steven Young-Uhk

College of Micronesia-FSM
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 1 - Advancing American Elderberry: Developing Innovative Value Added Products
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
American elderberry (Sambucus nigra subspecies canadensis) is native to much of North America. The fruit are soft, small, intensely purple drupes, containing three to five seeds. Berries exhibit some anti-viral properties and were found to slow the progression of dementia in animal studies. European elderberries (Sambucus nigra subspecies nigra) have long been used to make phytochemical-rich value-added products, in contrast to the underutilized American subspecies. American elderberry offers potential for products with more color stability as the berries have more cyanidin-3-sambuboside-5-glucoside and acylated anthocyanins than European elderberry. Developing innovative, cost effective, concentrated and portable products such as powders are desired for creating value added products. Successful microencapsulation of elderberry into powders was achieved by spray drying elderberry juice or pomace extract with soy protein isolate or tapioca starch as drying carriers. Anthocyanin profiles in the encapsulated powders were similar to those in juice but concentration was increased by 3-fold. Acylated anthocyanins made up 77% and cyanidin-3-sambuboside-5-glucoside, 17% of the total anthocyanins. Flavonoids delivered in powders encapsulated with soy protein concentrate were doubled in bioaccessibility compared to juice. Fermentation of juice with Saccharomyces cerevisiae yeast decreased soluble sugars and increased spray drying efficiency 20-25%. Fermented elderberry juice spray dried with pea protein or yeast as drying carriers showed increased phenolic content and bioaccessibility. This production strategy offers an efficient way to deliver stable, phytochemically enriched, value-added products from American elderberry for multiple food applications.
Speakers
avatar for Penelope Perkins-Veazie

Penelope Perkins-Veazie

Professor, NCSU
Penelope Perkins-Veazie, PhD, is a horticulture professor at the Plants for Human Health Institute. She studies postharvest physiology, which evaluates the best ways to keep fruits and vegetables edible after harvest. Her work includes measuring phytochemicals in produce at various... Read More →
Co-authors
AT

Andrew Thomas

University of Missouri
NA
JX

Jia Xiong

North Carolina State University
NA
KR

Karisma Ravichandran

University of Missouri
NA
KK

Kiruba Krishnaswamy

University of Missouri
NA
MG

Michael Greenlief

University of Missouri
NA
SS

S Silva

NCSU
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Viticulture and Small Fruits 1
  • Subject Viticulture and Small Fruits
  • Funding Source USDA-NIFA Specialty Crop Research Initiative Award number 2021-51181-35860
  • Funding Option SCRI funded all or part of the research associated with this abstract

1:00pm CDT

VSF 1 - Advancing Texas Horticulture: Bridging History and Innovation in Horticultural Crop Physiology at Texas A&M
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Texas has a strong history in fruit crop production, supported by its range of climates, soil types, and a long record of innovation. Grape growing began in the 1600s with Spanish missionaries and became globally important thanks to T.V. Munson, a horticulturist from Denison, Texas. His work in rootstock breeding helped save European vineyards during the phylloxera crisis. Today, Texas is a major producer of grapes, as well as pecans, peaches, citrus, and many small fruits. These crops all face challenges related to extreme weather events, soil conditions, and long-term sustainability. To help address these issues, the Horticultural Crop Physiology program at Texas A
Speakers
avatar for Lorenzo Rossi

Lorenzo Rossi

Assistant Professor, Texas A&M University
Dr. Rossi’s research program focuses on understanding the responses of horticultural crops to environmental stresses, with the goal of developing environmentally sound and effective management strategies. He is a horticulturist with expertise in plant stress physiology, plant biology... Read More →
Co-authors
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 1 - Growth and Yield Response of Two Native Aronia Species Grown in Different Ecoregions
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Aronia berry, also known as chokeberry, are rich in antioxidants that promote cardiovascular health, immune function and overall well-being. Beyond their nutritional benefits, they also serve as attractive ornamental landscape plants, providing wildlife provisioning and contributing to ecosystem services. However, not much is currently known about the plant growth and fruit quality of aronia berry in the southeastern US, and how different regions may affect plant growth characteristics. The main objective of this study was to establish plantings in two locations in Georgia and assess native aronia species for plant growth and fruit quality characteristics. The growth and yield of two native species, black chokeberry (Aronia melanocarpa) and red chokeberry (Aronia arbutifolia), were assessed during the 2024 growing season at two agroecological regions in Georgia: Blairsville (Zone 7a, Blue Ridge region) and Griffin (Zone 8a, Piedmont region). Field plots, consisting of three-year-old plants, were established at both locations in 2021. Plant measurements included: growth index, stem diameter at 100 cm height, yield (kg), berry size of the equator (mm), berry weight (g), dry berry weight (g), total soluble solids (°Brix), pH, and total titratable acidity (%TTA). Black chokeberry exhibited significantly higher values (p ≤0.05) for growth and yield in Blairsville than in Griffin. Total soluble solids were not different across locations. Similarly, red chokeberry had higher growth index, stem diameter, and yield in Blairsville, yet location had no effect on berry weight. Individual berry size was not affected by location for both black and red chokeberry. These findings suggest that even within adjacent eco-regions, aronia species show different growth and berry production, and that plants grown in northern regions exhibit larger size and bear more berries. This has implications for ornamental landscapes and restoration habitat practices. This work will be used as part of a larger work to examine the potential of aronia as an alternative fruit crop in the southeast.
Speakers
TA

Taiwo Addesyian

University of Georgia
NA
Co-authors
LL

Leynar Leyton

University of Florida
RI

Rachel Itle

University of Georgia
NA
SP

Svoboda Pennisi

University of Georgia
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 1 - Harvesting Sunlight Twice: Integrating Strawberries into Agrivoltaic Systems
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
The integration of solar energy production with agriculture, known as agrivoltaics, presents a promising solution to address land-use competition while boosting perennial crop productivity. This study evaluated the feasibility and impact of agrivoltaic systems on strawberry (Fragaria × ananassa) production in Iowa. The objectives included assessing plant growth, fruit yield and quality, microclimatic modifications, and economic viability under photovoltaic (PV) panel shading. We hypothesized that partial shading from PV panels would mitigate heat stress, reduce water demand, and enhance fruit quality without significantly compromising yield. A replicated field trial of two June-bearing strawberry cultivars in 2024 and 2025 compared plants under a standard rotating 5-ft PV configuration with traditional open-field production. Environmental parameters such as light intensity and temperature were monitored to quantify microclimatic changes. Additionally, plant physiological responses and post-harvest disease incidence were analyzed. Future economic assessments will determine profitability and trade-offs associated with energy co-production. Findings from this research informed best management practices for integrating agrivoltaics in specialty crop production, particularly in temperate regions. In 2024, berries were larger in weight and size under the panels compared to the open-field plots. Post-harvest assessment indicated that the increase in size did not correlate with a reduced shelf life. Overall, this study found a favorable effect of intermittent shading on strawberry production in the Midwest.
Speakers
LA

Laith Alomari

Iowa State University
NA
Co-authors
BD

Brooke Dietsch

Iowa State University
NA
SS

Suzanne Slack

Iowa State University
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 1 - Strawberries That Last: Smarter Nutrition and Longer Seasons for Small Farms on the Delmarva
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
This study presents the outcomes of the NE-SARE Professional Development Program, which was designed to enhance the capacity of farmers and agricultural service providers in the area of nutrition management for June Bearer (JB) and Day Neutral Strawberry (DNS) cultivars. Through seven targeted workshops including the UMES Small Farm Conference and the NE-SARE workshop on fertilizer strategies participants gained practical skills in fertilizer injector use, dose calculations, and the benefits of liquid vs. granular fertilizers. A total of 87 participants attended, with 82 identifying as Black and 46 as women. Key innovations included the distribution of over $7,000 worth of strawberry plants, overcoming access barriers for small-scale and BIPOC growers. Educational sessions transformed participant confidence, with post-survey data showing a shift from 40% reporting "no knowledge" to 70% achieving "advanced" proficiency in nutrition management. Participants reported the ability to perform fertilization calculations, use injectors, and understand fertilizer types. These workshops promoted sustainable practices by encouraging injector-based applications to reduce nutrient loss and pollution. This initiative not only advanced technical knowledge but also equipped growers to make environmentally sound, cost-effective decisions tailored to their operations. The outcomes reveal increased regional engagement and strengthened capacity among underserved farmers, empowering them to adopt sustainable techniques in strawberry cultivation on the Delmarva Peninsula.
Speakers
ND

Naveen Dixit

University of MD Eastern Shore
NA
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 1 - Tracking Pine Bark Decomposition in the Southern Highbush Blueberry Rhizosphere
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Growing southern highbush blueberries (SHB, Vaccinium corymbosum interspecific hybrids) in Florida’s sandy soils presents significant challenges due to their low water retention and limited fertility. Milled pine bark is a widely used soil amendment for SHB cultivation, as it enhances moisture retention, nutrient-holding capacity, soil structure, and maintains an optimal pH (approximately 4.0–5.0) for the crop. However, the physical and chemical characteristics of pine bark, particularly its decomposition dynamics within the SHB rhizosphere, remain poorly understood. This study investigates how pine bark decomposition affects SHB plant growth and productivity. SHB cultivars ‘Optimus’ and ‘Farthing’ were grown in raised beds amended with either aged or fresh pine bark. Plants were irrigated and fertigated via a drip system following commercial production practices. Beginning in June 2024, we measured plant volume, vertically projected leaf area, leaf nutrient concentrations, and yield. In addition, soil and pine bark samples were collected and analyzed. Our findings indicate that plants grown in aged pine bark produced greater above-ground biomass and exhibited higher macronutrient concentrations than those grown in fresh pine bark. The carbon-to-nitrogen (C:N) ratio differed between treatments at the start of the experiment, but fresh pine bark has changed more than aged pine bark since the start of the experiment. These results suggest that fresh pine bark may immobilize nutrients in the SHB rhizosphere, limiting plant growth. A deeper understanding of pine bark decomposition in SHB farms will support the development of sustainable fertilization strategies and improve long-term plant health and productivity.
Speakers
PL

Paige Larsen

University of Florida
NA
Co-authors
GN

Gerardo Nunez

University of Florida
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Viticulture and Small Fruits 1
  • Subject Viticulture and Small Fruits
  • Funding Source Florida Department of Agriculture and Consumer Services - Office of Agricultural Water Policy (Contract # 29958)

1:00pm CDT

VSF 1 - Understanding the Spatial and Temporal Regulation of Ripening in Blueberries
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Blueberries (Vaccinium species) belong to the Ericaceae family and are recognized for their antioxidant content and associated health-promoting properties. A key challenge in blueberry production is their asynchronous ripening with fruits on the same plant often maturing at different times. This leads to multiple harvests, increased production costs, and reduced overall fruit quality. Fruit ripening is a coordinated process wherein there is an increase in sugar concentration, decrease in acidity and accumulation of pigments such as anthocyanins. Blueberry fruit are classified as exhibiting atypical ripening behavior with an increase in respiration and ethylene during ripening. However, ethylene production is not autocatalytic and is under developmental regulation. Previous work from blueberry and other fruits have suggested a role of other hormones such as abscisic acid (ABA), auxin and jasmonates in fruit ripening. In this study, the cultivar Powderblue (Vaccinium ashei) was used to characterize the progression of ripening across multiple tissue types and developmental stages by examining the expression of key ripening-related genes. Fruits were harvested at five developmental stages including ripening. Ethylene was measured from these fruits to identify the stages that coincided with ripening initiation. For each stage, 9 tissue types were collected via dissection to obtain spatial resolution. RNA will be extracted from these tissues for qRT-PCR analyses to determine the expression of a focused-set of ripening related genes. For this study, a total of 37 genes were selected based on a previous RNA-Sequencing study that displayed a differential pattern in their transcript abundance during ripening. A subset of these genes is related to sugar, acid, anthocyanin, phenylpropanoid, photosynthesis, and cell wall metabolism. Further genes related to phytohormone metabolism related to ethylene, ABA, auxin, and jasmonates were included. Also, genes belonging to the NAC and MADS-box family of transcription factors were included. Based on the gene expression analysis, hormones including ABA, auxin and jasmonates will be quantified from a subset of these samples. This study will provide insights into the spatial context of molecular and hormone regulation. The knowledge from this study may help breeding programs aimed at developing cultivars exhibiting synchronous ripening, and the potential development of growth regulators to control ripening in the field.
Speakers
AS

Ashley Sorrow

University of Georgia
Co-authors
AM

Anish Malladi

University of Georgia
RS

Ranveer Singh

University of Georgia
NA
SN

Savithri Nambeesan

University of Georgia
Tuesday July 29, 2025 1:00pm - 1:45pm CDT
Empire AB
 
Wednesday, July 30
 

9:00am CDT

UG 1 - Effect of Temperature, Smoke, and Chemical Treatment on Germination of White Sage
Wednesday July 30, 2025 9:00am - 10:00am CDT
White sage, Salvia apiana, is native to coastal areas of southern California and northern Baja California. Leaves and stems of white sage are ceremonially burned in many indigenous communities, and the plant has additional culinary and medicinal importance. While white sage is not endangered, over-harvest of wild sage is of concern for conservation efforts. Propagation of white sage is generally accomplished by seed; however, germination is poor with rates ranging from 10 to 25 percent. This project evaluated application of heat and smoke treatment for their impact on germination. Temperature and time of exposure were found to significantly impact germination with treatment at 70C and 85C leading to increased germination at 21 days. Application of smoke originating from charcoal and burned sage did not impact rate of germination in seed not exposed to heat treatments.
Speakers
SS

Sam Sarratt

University of Nebraska-Lincoln
NA
Co-authors
CE

Christian Elowsky

University of Nebraska-Lincoln
NA
RS

Ronald Stephenson

Plant Sciences Hall
Wednesday July 30, 2025 9:00am - 10:00am CDT
Empire AB

9:00am CDT

UG 1 - Genome Size Estimation of Eight Thimbleberry (Rubus parviflorus) Populations Across the United States
Wednesday July 30, 2025 9:00am - 10:00am CDT
Thimbleberry (Rubus parviflorus) is a shrub native to the western temperate regions of North America, from Alaska to California and the Rocky Mountains. Despite its ecological importance and broad native range, genomic information for this species remains extremely limited. So far, only one flow cytometry data point is available for thimbleberry, reporting a haploid nuclear DNA content (C value) of 0.28 pg, which suggests a haploid genome size of approximately 270 Mb. The Rubus genus exhibits a broad range of ploidy levels, such as from diploid to dodecaploid (2n = 2x = 14 to 2n = 12x = 84) for blackberries (R. argutus). Genome size variation has also been documented in other shrub species. In plants such as Chrysanthemum indicum, Linum suffruticosum, and Lycoris radiata, changes in ploidy levels have been associated with environmental stress. Given the wide native distribution of thimbleberry, we hypothesized that genome size variation may exist among different thimbleberry populations. The aim of this study was to estimate the genome size of eight thimbleberry populations collected from various locations across the United States, including the Northwest, Midwest, Southwest, and Rocky Mountains, using a flow cytometer. The findings from this study provide baseline genomic data that can support future research efforts, such as the construction of a reference genome and investigations into ploidy variation and its potential relationship with environmental stress tolerance.
Speakers
ST

Stirling Todd

Utah Valley University
Co-authors
CH

Carl Hjelmen

Utah Valley University
NA
MR

Michael Rotter

Utah Valley University
NA
YL

Yuya Liang

Utah Valley University
NA
Wednesday July 30, 2025 9:00am - 10:00am CDT
Empire AB

9:00am CDT

UG 1 - Genomic exploration of WUS and BBM in challenging-to-culture blueberry varieties
Wednesday July 30, 2025 9:00am - 10:00am CDT
Blueberry breeding faces substantial challenges in efficiently producing plants with desired traits through traditional methods, limiting the industry's ability to meet increasing demands. Tissue culture (TC) and regeneration methods, while promising, are hindered by high costs, cultivar-specific outcomes, and unpredictability. Recent research has identified the significant roles of morphogenic genes such as WUSCHEL (WUS) and BABY BOOM (BBM) in shoot regeneration, early shoot formation, and transformation processes. This study investigates the gene morphology and function of native BBM and WUS genes in various Vaccinium corymbosum (highbush blueberry) varieties exhibiting different levels of tissue culture recalcitrance. Previously, we identified the locations of WUS and two BBM gene variants (BBM1 and BBM2) in blueberry transcriptome data, followed by primer design, PCR, and Sanger sequencing. BBM variants and WUS were analyzed in blueberry varieties ‘ONeal’ and ‘Legacy,’ which are capable of propagation via TC, and ‘Pinnacle,’ ‘NC 4499,’ and ‘NC5271,’ which lack transcriptome data and are known to be recalcitrant to TC. Initial WUS sequencing revealed a three-nucleotide deletion in the hard-to-culture varieties ‘NC4499,’ ‘NC5271,’ and ‘Pinnacle,’ resulting in the loss of a single amino acid. Additionally, two non-synonymous SNPs with high mutation rates were identified in ‘Pinnacle’ and ‘NC5271.’ To further investigate the impact of these genetic differences, we conducted protein sequence analysis to determine how the codon deletion in WUS affects protein structure. This could uncover potential alterations in protein folding or function, which may contribute to reduced regenerative capacity in recalcitrant varieties. We are currently working to extract RNA from the five blueberry varieties at different growth phases for cDNA synthesis and qPCR to investigate gene expression levels and copy number. This project aims to address a critical gap in blueberry cultivation by uncovering the genetic basis for propagation challenges in high-value varieties. By focusing on the WUS and BBM genes, the study seeks to identify genetic variations contributing to TC resistance, informing future genetic and breeding strategies.
Speakers
EW

Ellen White

North Carolina State University
NA
Co-authors
HA

Hudson Ashrafi

North Carolina State University
NA
Wednesday July 30, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 1
  • Poster # csv
  • Funding Source NCBiotech and NC State University Genetics and Genomics Academy

9:00am CDT

UG 1 - Horticulture-Based Organic Agroforestry to Prevent Soil Erosion and Forest Fire, and to Keep the Humans and Mother Earth Healthy
Wednesday July 30, 2025 9:00am - 10:00am CDT
Agroforestry is the intentional integration of trees and shrubs into crop and animal farming systems to create environmental, economic, and social benefits. It helps prevent soil erosion by stabilizing the soil with tree roots, reducing wind and water runoff, and protecting the soil. Trees also mitigate forest fire risks by creating buffer zones and improving soil health by adding organic matter, enhancing water retention, and promoting beneficial microbial activity, which boosts soil fertility and Soil erosion and forest fires are significant global conservation challenges. This sustainable approach fosters synergies between plant and animal species to enhance biodiversity, prevent soil erosion, improve soil health, and increase farm productivity. The objective of this research is to evaluate the effects of a horticulture-based agroforestry system in preventing wind erosion, enhancing soil nutrients, improving soil quality, and increasing crop yields. Horticulture-based agroforestry refers to land-use systems where herbs, shrubs, and trees are grown for food on the same land unit without livestock. It is a mixed cropping system with annuals, biennials, and perennials, providing financial, environmental, and health benefits. This long-lasting conservation farming system includes cereals, fruits, vegetables, tubers, nuts, legumes, spices, and condiments. This agroforestry system will provide carbohydrates, protein, minerals, vitamins, anthocyanins, and flavonoids to the farming community. It is practiced in Asia and Africa for the economic sustainability of small farmers and the provision of nutritious foods. In our farming system, annual and biennial vegetables, muscadines, blueberries, corn, legumes, cover crops, ginger, cocoyam, and turmeric are raised. This agroforestry research program is the first of its kind in the nation, focusing on horticultural crops where pecans are tree crops. Five acres of land on Memphis Silt Loam soil were divided into five blocks for pecans, vegetables, muscadines, blueberries, spices, and condiments. The tree rows are widely spaced, with a plant-to-plant distance of 13.7 meters and a row-to-row distance of 27.4 meters, to prevent forest fires. Inter-cropping, inter-planting, mixed cropping, and cover cropping are practiced with organic manures for nutrient management and soil erosion prevention. Randomized complete blocks (RCBD) and completely randomized designs (CRD) were used to raise perennial crops with organic treatments. The C-factor research technology, developed by Alcorn State University in collaboration with USDA scientists, evaluates growth parameters including upper and lower biomass development once every 15 to 20 days. Soil samples at 15cm deep are collected every year to assess soil health, promoting economic and environmental sustainability.
Speakers
ET

Ellis Terrett

Alcorn State University
Co-authors
GP

Girish Panicker

Alcorn State University
Dr. Girish Kumar Panicker, Professor of Plant and Soil Sciences and Director of Conservation Research, at Alcorn State University, Mississippi, is an authority on cover and management (C-factor) research on horticultural crops and is an internationally renowned agricultural scientist... Read More →
WM

Willis Mims

Alcorn State University
NA
Wednesday July 30, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 1
  • Poster # csv
  • Funding Source Alcorn State University

9:00am CDT

UG 1 - Seed Propagation of Blue Elderberry (Sambucus cerulea)
Wednesday July 30, 2025 9:00am - 10:00am CDT
Blue Elderberry (Sambucus cerulea) is a fruiting plant native to Western North America, ranging from British Columbia to Northern Mexico, and from Oklahoma to California. Like the black-fruited S. nigra and S. canadensis, it has the potential to be an important alternative crop. However, S. cerulea is more difficult to asexually propagate, and requirements for seed germination are not well understood. Seeds from 5 Utah selections and 2 commercial black-fruited cultivars were collected during the 2023 and 2024 harvests to determine optimum stratification requirements. No germination occurred before 12 weeks of stratification at 2°C for any genotypes tested. Optimum stratification period differed among genotypes and ranged from 18 to 24 weeks. Germination rates also varied by genotype ranging from 13% for a wild-collected population to 63% for a selection established in a common experimental orchard. Possible correlation between seed stratification requirement and other phenological development indicators (time of budbreak, bloom and fruit ripening) is still being determined. Information on propagation and climatic adaptation will be important in developing S. cerulea as an alternative crop and identifying suitable locations for commercial production.
Speakers
SS

Stephanie Spencer

Utah State University
Co-authors
BB

Brent Black

Utah State University
KK

Kaylee Kowallis

Utah State University
NA
LY

Lauren Yardley

Utah State University
NA
Wednesday July 30, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 1
  • Poster # csv
  • Funding Source Utah State University

9:00am CDT

UG 1 - Soil Composition Effects on Growth and Physiological Performance of Dragon Fruit (Selenicereus undatus) Under Greenhouse Conditions
Wednesday July 30, 2025 9:00am - 10:00am CDT
Selenicereus undatus (dragon fruit) is a high-value crop experiencing increasing global demand, yet limited research exists on how soil composition influences its growth and physiological performance. This study evaluated the effects of five distinct soil substrates on two dragon fruit varieties under controlled greenhouse conditions. 120 plants were arranged in a completely randomized design with five replications per treatment. The five soil treatments included Native South Texas Soil, 100% Sandy Soil, Sandy Soil 25% Sphagnum, Sandy Soil 50% Sphagnum, and 100% Sphagnum. Initial and final analyses of soil pH, salinity, texture, and nutrient content were conducted to monitor soil dynamics. Shoot length was measured at 0, 30, 60, and 90 days, while root biomass and architecture were assessed at harvest. Physiological traits, including chlorophyll concentration and total biomass, were also recorded at the conclusion of the experiment. Results revealed that soil composition influenced both above- and below-ground growth, with soils amended with sphagnum showing moderate increases in shoot elongation and chlorophyll content compared to unamended sandy and native soils. Root development varied across treatments, though no statistically significant differences in total biomass were detected. Variations in soil pH and organic matter content appeared to correlate with some physiological markers, suggesting a complex interaction between soil properties and plant response. While additional studies are needed to explore long-term productivity and fruit quality, these findings provide preliminary insights into how soil amendments may influence early-stage development of dragon fruit. This work contributes to ongoing efforts to optimize substrate management for dragon fruit cultivation, particularly in regions with poor native soils, such as South Texas.
Speakers Co-authors
LR

Lorenzo Rossi

Texas A
Dr. Rossi’s research program focuses on understanding the responses of horticultural crops to environmental stresses, with the goal of developing environmentally sound and effective management strategies. He is a horticulturist with expertise in plant stress physiology, plant biology... Read More →
Wednesday July 30, 2025 9:00am - 10:00am CDT
Empire AB

10:30am CDT

UG 2 - Achieving Faster Pepper Hybridization Potential by Light Intensity and Duration Manipulation
Wednesday July 30, 2025 10:30am - 11:40am CDT
Chile peppers (Capsicum spp.) have traditional breeding cycles requiring hybridizations and then multiple generations of backcrossing and/or inbreeding, over several years, to establish genetically stable cultivars. Accelerating breeding cycles is essential to address global agricultural and industrial demands. Light conditions, specifically photoperiod length and luminous intensity, play a crucial role in plant development and serve as key factors in optimizing breeding efficiency. This study examines the effects of manipulating photoperiod and light intensity on four C. annuum genotypes: NuMex Lotalutein, NuMex Odyssey, NuMex Las Cruces, and Early Jalapeno. There were 5 plants per genotype per treatment, one treatment being normal greenhouse conditions and another a growth chamber with LED grow lights. Plants were monitored for key growth and developmental metrics, including germination speed and rates, and days to flowering. The measurements were recorded and analyzed using Tukey’s HSD in R (⍺ = 0.05). Results indicate that manipulating light duration and intensity promotes faster germination and earlier flowering, potentially expediting hybridization processes within breeding programs. By reducing generation time, these findings suggest that targeted light treatments can significantly accelerate cultivar development, facilitating more efficient breeding strategies for Capsicum improvement.
Speakers
UM

Uriel Martinez

New Mexico State University
Co-authors
AV

Amanda Velazco

New Mexico State University
NA
DN

Dennis Nicuh Lozada

New Mexico State University
SN

Shahab Nourbakhsh

New Mexico State University
Wednesday July 30, 2025 10:30am - 11:40am CDT
Empire AB

10:30am CDT

UG 2 - Comparative Observation of Trichome Type and Density in Solanum spp. Using Scanning Electron Microscopy
Wednesday July 30, 2025 10:30am - 11:40am CDT
Trichomes play vital roles in plant defense and environmental regulation, including protection from UV rays and herbivores, as well as helping to manage surface temperature and reduce water loss. There are two main types of trichomes, glandular, which produce secondary metabolites such as alkaloids, flavonoids, and terpenes, and non-glandular, which are primarily involved in physical protection and regulation. This study aims to document and compare trichome type, density, and morphology in both wild and cultivated Solanum (tomato) species. Two wild species and two cultivated varieties were grown in a greenhouse, and leaf samples are collected during the vegetative growth stage. Small circular cuttings from mature leaves are air dried and examined under a Tescan Vega3 scanning electron microscope (SEM). Data was collected on trichome type, ratio, density, and morphology for each variety. This comparative analysis provides foundational information about trichome structure across tomato species, which may relate to differences in environmental adaptability or natural pest resistance.
Speakers
DP

Dalton Palmer

Utah Valley University
Co-authors
JR

Jeremy Redd

Utah Valley University
NA
YL

Yu-YA Liang

Utah Valley
NA
Wednesday July 30, 2025 10:30am - 11:40am CDT
Empire AB

10:30am CDT

UG 2 - Evaluating the Effect of Selenium Biofortification on Beta-Carotene Accumulation in Upland Cress Grown in a Greenhouse
Wednesday July 30, 2025 10:30am - 11:40am CDT
Nutrient biofortification through micronutrient supplementation offers a practical approach to enhancing the nutritional profile of leafy vegetables while promoting sustainable food production. Selenium (Se), a vital trace element for human health, plays a significant role in plant metabolism and the synthesis of antioxidant compounds such as beta-carotene. However, just a few research has explored selenium's effect on beta-carotene accumulation in upland cress (Barbarea verna) under controlled greenhouse conditions. This ongoing study evaluates the effect of selenium biofortification on beta-carotene accumulation in upland cress using a deep-water hydroponic system. The experiment is laid out in a randomized complete design with a control and three selenium treatment levels (0, 1.0, 2.0, and 4.0 mg Se·L⁻¹), corresponding to control, low, medium, and high concentrations. Sodium selenate serves as the selenium source. Key growth parameters and environmental conditions such as pH and electrical conductivity (EC) of the nutrient solution are monitored throughout the cultivation period. Half Strength Hoagland solution is being used as a nutrient solution for this research. Plant samples will be analyzed to determine the relationship between selenium uptake and beta-carotene content using spectrophotometric analysis. This research aims to identify the optimal selenium concentration that enhances beta-carotene synthesis without compromising plant growth or yield. Preliminary observations suggest a dose-dependent physiological response to selenium supplementation, which may contribute to biofortification strategies for functional leafy greens. Findings from this study will inform controlled environment agriculture practices and support the development of nutrient-enriched crops for improved human health. Keywords: Selenium biofortification, Beta-carotene, Upland cress, Hydroponics, Controlled environment, Greenhouse production, Functional foods, Nutritional enhancement.
Speakers
AA

Adekunle Adeyeye

Kentucky State University, Frankfort, KY
Co-authors
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Wednesday July 30, 2025 10:30am - 11:40am CDT
Empire AB

10:30am CDT

UG 2 - Identifying Heat Tolerant Root Stocks Out of Eight Different Tomato Cultivars Grown at Elevated Temperatures
Wednesday July 30, 2025 10:30am - 11:40am CDT
Identifying Heat Tolerant Root Stocks Out of Eight Different Tomato Cultivars Grown at Elevated Temperatures Samantha Buie, Courtney Dekalb-Myers, Shivani Kathi, Justin Moss Department of Horticulture and Landscape Architecture, Stillwater, Oklahoma, 74078 *Presenting author- Samantha.buie10@okstate.edu Heat stress is a common challenge when it comes to tomato production in Oklahoma. High temperatures affect different physiological and biochemical functions in tomato plants, thereby affecting plant growth, yield, and fruit development. The current study aims to identify the heat tolerant tomatoes and their potential for root stocks out of eight tomato varieties. Different varieties of tomatoes include ‘Celebrity’, ‘Florida 91’, and trial varieties ‘NC 2/270’, ‘NC 2/271’, and ‘NC 2/272’ which are considered heat tolerant, while ‘Homestead’ and ‘Rutgers’ are considered heat sensitive. These varieties were replicated four times in completely randomized design in two different growth chambers. One growth chamber was maintained at an optimum temperature (26 °C/ 19 °C Day/night) and the other growth chamber was maintained at elevated temperature conditions (36 °C/ 26 °C). Data was collected on plant height, number of leaves, side shoots, flowers, and fruits, and plant and root biomass at harvest. The heat tolerant tomato cultivars had better plant and root characteristics than that of heat sensitive tomatoes. Identification of superior root stocks will help researchers use them in combination with better performing scions in future studies to develop heat-tolerant grafted tomato plants with improved growth and yield.
Speakers
SB

Samantha Buie

Oklahoma State University
Co-authors
CD

Courtney DeKalb Myers

Oklahoma State University
JM

Justin Moss

Horticulture and Landscape Architecture
NA
SK

Shivani Kathi

Oklahoma State University
Wednesday July 30, 2025 10:30am - 11:40am CDT
Empire AB

10:30am CDT

UG 2 - Reducing leaching during short-cycle, plant production reduces phosphorus losses
Wednesday July 30, 2025 10:30am - 11:40am CDT
Short-cycle, plant production requires frequent irrigation and readily available nutrients to support plant growth. However, excess irrigation can lead to higher leach volumes and nutrient losses that can slow plant growth while increasing offsite nutrient movement into sensitive areas. The objective of this study was to evaluate the effect reducing leaching has on P losses during coleus (Plectranthus scutellarioides) production over a four-week production cycle. Coleus plugs were grown in 3-quart containers filled with a 3 pinebark:1 peat:1 vermiculite substrate amended with 0.3 kg m–3 N and P2O5 kg m-3. Irrigation was applied at 298 mL (1.05 cm) during a single two-minute cycle per day as a standard treatment versus coleus grown under a sensor-controlled irrigation system that ceased application once leaching occurred. Leachate was captured in one plant per replication for weekly measurement of pH, EC, and DRP. No differences in coleus growth index or shoot and root biomasses at 4 weeks after planting (WAP) occurred between coleus following either irrigation regimen. However, coleus subject to the sensor-controlled irrigation resulted in reductions in cumulative irrigation and leachate volumes of 40% and 69%, respectively, that led to a 45% reduction in cumulative DRP leached compared to coleus following the standard timed irrigation regimen. Limiting irrigation to reduce the incidence and volume of leaching can significantly decrease DRP losses without sacrificing coleus growth.
Speakers
EB

Evelyn Boakye

UNCP
I am an undergraduate student
Co-authors
BR

Braydann Revels

UNCP
I’m an undergraduate student majoring in biology with a biomedical emphasis.
Wednesday July 30, 2025 10:30am - 11:40am CDT
Empire AB

10:30am CDT

UG 2 - Solarization of substrate using a solar oven to reduce weed seed competition
Wednesday July 30, 2025 10:30am - 11:40am CDT
Weed seed contamination of substrates often leads to weeds competing with the desired plant species for essential resources. The objective of this research was to evaluate if a solar oven provides a simple sustainable, solarization method for reducing weed competition in small batches of contaminated pine-bark based substrate. In the laboratory, petri dishes containing 100 weed seeds per plate that were subjected to 15 or 30-min heat treatments at 45, 60, and 75°C using a electric oven. Non-heat-treated seeds served as controls. Heat treated seeds and controls were then germinated in a growth chamber over twenty-one days. Weed seeds that were heat treated at higher temperatures for 30-min resulted in declining germination rates, while weed seeds exposed to corresponding temperatures for 15-min exhibited less efficacy in reducing germination. Based on the laboratory results, a field experiment was setup to evaluate solarization of a weed seed contaminated substrate using a solar oven. Substrates were mixed with 100 weed seeds per replication and subjected to >65°C for 30-min. Substrates were placed under greenhouse conditions to measure seed emergence. Weed seed emergence declined after solarization, however, performance of the solar oven was dependent on environmental fluctuations.
Speakers
BR

Braydann Revels

UNC-Pembroke
I’m an undergraduate student majoring in biology with a biomedical emphasis.
Co-authors
EB

Evelyn Boakye

UNCP
I am an undergraduate student
Wednesday July 30, 2025 10:30am - 11:40am CDT
Empire AB

10:30am CDT

UG 2 - Variety Trial of Hydroponic Brassica oleracea (kale) Across Different Systems and Natural Light Exposure
Wednesday July 30, 2025 10:30am - 11:40am CDT
The aim of this project is to take seven different cultivars of Brassica oleracea (kale) and quantify their performance based on morphological and yield data and compare the results when planted in either west or east facing greenhouses and either Deep Water Culture (DWC) or Nutrient Film Technique (NFT) hydroponic systems. Two identical greenhouses were set up with both a DWC and NFT inside, one facing east and one west. Seedlings were started in rockwool under the same conditions for 21 days in both greenhouses, then moved to either the DWC or NFFT system and left to grow for 25 days in similar fertilizer and pH levels. When comparing consumable plant mass (leaves) across these variables, the plants in the east facing bay had a 38% increased size compared to the plants in the west facing, the plants in the DWC had a 29% increase compared to the NFT. The varieties KX-1 and Oldenbor were the top performers across several categories including low size, high weight, and uniformity.
Speakers
DV

Devin Vitello

University of Tennessee Knoxville
I am an undergraduate researcher under Dr. Carl Sams here at The University of Tennessee Knoxville. I am majoring in Plant Sciences with a concentration in Horticulture and Production, and I have a minor in Food and Agriculture Business.
Wednesday July 30, 2025 10:30am - 11:40am CDT
Empire AB
  Poster, Undergraduate Student 2
  • Poster # csv
  • Funding Source AURA

12:15pm CDT

CE 2 - Active Aeration Increases Biomass by Enhancing Water Uptake in Greenhouse Hydroponic Lettuce and Influences Efficacy of a Calcium-Mobilizing Biostimulant
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Tipburn is a physiological disorder that compromises the appearance and marketability of lettuce (Lactuca sativa) by causing necrosis and curling at leaf margins. It is problematic in controlled-environment hydroponic systems, where calcium demand outpaces calcium supply. Previous research demonstrated that nutrient solution application of a calcium-mobilizing biostimulant curbed lettuce tipburn. However, it remained unclear whether its efficacy depended on nutrient solution dissolved oxygen (DO), which is crucial for water and nutrient uptake. We evaluated effects of this calcium-mobilizing biostimulant and the DO level on growth and tipburn severity of hydroponic butterhead lettuce ‘Nancy’. Seeds were planted in a temperature-controlled greenhouse under a 24-hour photoperiod with supplemental white red light-emitting diodes providing a mean photosynthetic photon flux density of 300 μmol⋅m–2⋅s–1. After 11 days, seedlings were transplanted into deep-water-culture trays in the same greenhouse at 22.7 ± 4.1 °C air temperature and 23.6% ± 5.1% relative humidity, with the same continuous supplemental lighting. In a randomized complete block design with three blocks, plants were grown in nine nutrient solution treatments per block, which were combinations of three biostimulant concentrations (0, 0.25, and 0.5 mL⋅L–1) and three DO levels (2.9, 3.5, and 4.0 mg⋅L–1). The low DO level was achieved without active aeration, whereas the medium and high DO levels were achieved with active aeration by adjusting airflow rates. Plants were evaluated on 21 and 28 days after transplant (DAT). On 21 DAT, plant growth benefited from active aeration but was unaffected by the biostimulant. Compared with no active aeration, active aeration increased shoot fresh mass by 127% to 139% and moisture content by 5% to 7%, but did not affect shoot dry mass. This indicates that growth promotion was attributed to increases in both relative and absolute water uptake, but not whole-plant photosynthesis, despite increased canopy size. The biostimulant decreased the percent of tipburn-affected leaves from 33% to 12% under the medium DO level. On 28 DAT, shoot fresh mass, shoot dry mass, moisture content, and canopy size had similar trends to those on 21 DAT. The high biostimulant concentration decreased the tipburn rating by 36% and the number of tipburn-affected leaves by 50% without active aeration, but not with it, indicating that the biostimulant was more efficacious under root hypoxia than under sufficient DO levels. We conclude that active aeration enhances lettuce water uptake and shoot fresh mass, whereas the biostimulant moderately controls tipburn at lower DO levels.
Speakers
JR

Jonathan Ries

University of Delaware
Co-authors
QM

Qingwu Meng

University of Delaware
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 2
  • Poster # csv
  • Funding Source This work is supported by the Urban, Indoor, and Emerging Agriculture Program (project award no. 2023-70019-39371) from the U.S. Department of Agriculture’s National Institute of Food and Agriculture. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and should not be construed to represent any official USDA or U.S. Government determination or policy.

12:15pm CDT

CE 2 - Effect of Constant Air Flow on Lettuce Morphology and Yield in Vertical Farming Systems
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Lettuce is the most cultivated leafy vegetable under vertical farming due to its short growth period and stable market demand. However, lettuce production in those systems often faces challenges such as unequal temperature inside cultivation room and benches, which causes uneven growth, leading to cultivation delays and yield loss. In this study, we investigated the effect of constant air flow on lettuce production in vertical farming to observe changes in morphology and yield. The aim of this study was to improve leaf lettuce production in vertical farms. Leaf lettuces were grown at Advanced Plant Factory Research Center, Meiji university under temperature of 22°C, relative humidity of 60%, under white LED light at a PPFD of 140–200 µmol·m⁻²·s⁻¹ for 16 hours. The hydroponic solution was supplied to the seedlings by the deep flow technique at an EC of 1.6 dS·cm⁻¹, pH of 6.0±0.5. For the air flow treatment, air tubes were placed above the seedlings, a constant wind speed of 0.1-0.2 m/s for 24 hours was supplied to seedlings. At 10, 20, and 30 days after transplanting (DAT), lettuces from both the control and air flow treatment groups were selected for stomata evaluation using a non-destructive equipment. At 30 DAT, yield evaluation, including fresh weight, leaf count, and morphology change such as leaf thickness, was also conducted. We found that the constant air flow treatment increased the fresh weight and leaf count of the lettuces. Furthermore, an increase in leaf hardness was observed. Moreover, we found that the number of stomata tended to be higher when the fresh weight was greater. This tendency was particularly strong in the air flow group which proved that it improved the growth and morphological characteristics of lettuce, suggesting its potential for enhancing vertical farming productivity.
Speakers
JS

Junjira Satitmunnaithum

Specially-appointed lecturer, Organization for the Strategic Coordination of Research and Intellectual Properties, Meiji University , Japan
Co-authors
CI

Chiharu Inoue

Daiwa house industry
NA
SO

Sakurako Otani

Meiji university
TO

Takao Otsuka

Daiwa house industry
NA
TI

Takashi Ikeda

Meiji university
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CE 2 - Exploring Hydroponic Head Lettuce Performance: Deep Water Culture vs. Vertical Production Systems
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Hydroponic farming has emerged as a promising solution to global food security challenges, with the market projected to reach $12 billion by 2025. Two commonly used systems are floating raft deep water culture (DWC) and vertical nutrient film technique (NFT) production systems. Despite their popularity, limited research exists comparing water use efficiency and Photosystem II (PSII) performance between these systems. This study begins to address this gap by evaluating the growth of Butterhead lettuce in both DWC and vertical NFT systems within a controlled greenhouse environment. PSII physiological parameters were assessed using a handheld fluorometer (MultispeQ Version 2 – PhotosynQ). Crop-level water use efficiency was also compared between systems. As expected, preliminary results indicated that the DWC system was more efficient in its water use than the vertical NFT system (p < 0.05), after adjusting for baseline water loss prior to plant introduction. More importantly, lettuce grown in the DWC system exhibited a higher mean PSII efficiency (Fv’/Fm’) and greater wet biomass compared to those grown in a vertical system. This study is among the first to directly compare PSII-related traits across hydroponic system designs, providing novel insights into how production method influences plant physiological performance. These findings suggest that DWC systems may offer superior performance, improved water use efficiency, and higher yields, making them a more sustainable and effective alternative for hydroponic lettuce production compared to vertical NFT systems.
Speakers
IS

Isaiah Spiegelberg

University of Wyoming
Co-authors
CG

Carmela Guadagno

University of Wyoming
NA
SD

Sarah Doyle

University of Wyoming
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 2
  • Poster # csv
  • Funding Source NSF

12:15pm CDT

CE 2 - Growth and Nutrient Dynamics in Salanova Lettuce: Evaluating Biofortification Potential in Field and Deep-Water Systems
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Enhancing the nutritional quality of leafy greens is a key goal in horticultural research, with biofortification strategies offering potential benefits for human health. This study examines the effects of foliar ascorbic acid (vitamin C) applications on the growth and nutrient composition of Salanova lettuce (Lactuca sativa) in both deep-water hydroponic and field production systems. Lettuce plants were subjected to four treatment concentrations (0, 500, 750, and 1000 ppm ascorbic acid), applied as foliar sprays at two and four days before harvest. Growth parameters, including biomass accumulation over time and final fresh weight, were recorded for both systems. Chlorophyll content before and after treatment applications were compared to assess potential physiological responses. Vitamin C content analysis is ongoing to determine potential biofortification effects. Results indicated no significant negative impacts on plant width among treatments in both field and deep-water systems, or on final biomass among treatments in the field. A block effect was observed in the final weight for the deep-water system, suggesting environmental or positional influences within the setup. Chlorophyll content in the deep-water system was higher in the 0 and1000 ppm treatments and lowest in the 750ppm treatment, showing that there may be slight impacts on lettuce quality. However, no impacts of treatment on chlorophylls were seen in field trials. These results show that ascorbic acid sprays up to 1000 ppm do not negatively impact lettuce quality. Further analysis will determine whether short-term nutrient enhancement through biofortification is viable. These findings contribute to understanding nutrient management strategies for hydroponic and field-grown lettuce and their potential role in sustainable production systems.
Speakers
KS

Kaitlyn Stubbs

Texas Tech University
Co-authors
AR

Abishkar Regmi

Texas Tech University
CC

Carlos Carpio

New Mexico State University
NA
CS

Catherine Simpson

Texas Tech University
CW

Chenggang Wang

Texas Tech University
NA
KV

Katerina Vargas

Texas Tech University
LT

Leslie Thompson

Texas Tech University
NA
NM

Naima Moustaid-Moussa

Texas Tech University
NA
SS

Sukhbir Singh

Texas Tech University
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 2
  • Poster # csv
  • Funding Source Texas Tech University Pursuit of Prime

12:15pm CDT

CE 2 - The Water Vapor-pressure Deficit Regulates Growth of Culinary Herbs and Lettuce in Controlled Environments
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
This experiment aims to quantify the effects of the water vapor-pressure deficit (VPD) on growth and quality of culinary herbs and lettuce grown inside controlled environments. We postulated that growth will be inhibited at very low and very high VPDs when all other environmental factors are kept constant. We sowed seeds of holy basil (Ocimum tenuiflorum), chamomile (Matricaria chamomilla), lettuce (Lactuca sativa ‘Rex’), mint (Mentha spicata), and lemon balm (Melissa officinalis) in plug trays containing a peat-based soilless substrate. Seedlings grew inside refrigerated chambers at 23°C, with 24-h photoperiod at a photosynthetic photon flux density of 160 µmol∙m–2∙s–1, at five VPD setpoints (kPa): 0.3, 0.7, 1.1, 1.5, and 1.9. We fertigated plants with reverse-osmosis water at a constant fertility of 60 mg N∙L–1. We measured the total leaf area, leaf number, stem length, fresh shoot biomass, and dry shoot biomass, and monitored leaves for tip-burn symptoms. Preliminary results indicate that leaf area and fresh mass of most species were lower at a VPD of 0.3 kPa than at ≥0.7 kPa. In some species (e.g., mint), plants were shortest at the lowest VPD tested, whereas in others (e.g., chamomile), stem length decreased as the VPD increased. These results indicate the importance of managing VPD in controlled environments and especially the potential decreases in growth under low a VPD (i.e., high relative humidity).
Speakers
AT

Avery Tucker

Michigan State University
Co-authors
ER

Erik Runkle

Michigan State University
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

MKEC - Beyond Plastic: Consumer Awareness as a Catalyst for Biodegradable Plastic Acceptance in Agriculture
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Soil-biodegradable plastic mulches (BDMs) are an alternative to non-biodegradable plastic mulch films. BDMs reduce agricultural plastic waste generation while maintaining many of the horticultural benefits of traditional mulch film. Yet, many farmers are reluctant to adopt BDMs. Previous research suggests that this hesitancy stems, in part, from farmers’ belief that consumers may be concerned about the unsightly plastic fragments left behind as BDMs biodegrade. Some farmers fear these remnants could be viewed by consumers as indicative of “bad farming” practices. However, consumers’ actual perceptions have not been evaluated. The objective of this research was to explore consumers’ perceptions of BDM fragments in farm fields. A rapid market assessment (RMA) paired with exploratory interviews were conducted with consumers at a farmer’s market in Northwest Washington State in Winter 2024. Of the 50 people who completed the farmers’ market RMA, 51% and 14% disagreed or strongly disagreed, respectively, that growing produce is associated with plastic use. Moreover, approximately 80% of the respondents indicated they did not think plastic use was essential to modern agriculture. Most respondents agreed (51%) or strongly agreed (12%) that farm visual cleanliness is important and influences their willingness to support a farm with their business. All respondents (100%) did not like seeing plastic waste on farms. Nearly all (96%) expressed a preference for biodegradable alternatives to non-biodegradable agricultural plastics, which is noteworthy given that 66% of consumers had prior experience or knowledge of biodegradable plastics. These findings, along with the exploratory interview results, suggest that consumers are more accepting of soil-biodegradable plastic scraps in farm fields, especially when they are aware the material is biodegradable and used as an eco-friendly alternative to non-biodegradable plastics.
Speakers
AW

Aidan Williams

Washington State University
Co-authors
JG

Jessica Goldberger

Washington State University
NA
LW

Lisa Wasko DeVetter

Washington State University
Lisa Wasko DeVetter joined Washington State University in 2014 and leads the small fruit horticulture program at the Northwestern Washington Research and Extension Center in Mount Vernon, Washington. She has developed a diverse research and extension program with an emphasis on maximizing... Read More →
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Marketing and Economics
  • Subject Marketing and Economics
  • Poster # csv
  • Funding Source USDA Agriculture and Food Research Initiative Competitive Grants Program Foundational and Applied Science Program

12:15pm CDT

MKEC - Comparing Conventional In-ground Tomato Production to Hydroponic Tomato Systems.
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
The rapid increase in the global population has led to a decline in land availability for agricultural production, resulting in food insecurity and exacerbating environmental challenges such as soil degradation, deforestation, and biodiversity loss. In-ground farming faces growing challenges, including declining soil fertility, water scarcity, and vulnerability to climate change, along with issues like soil erosion, nutrient depletion, and heavy reliance on chemical inputs. In response, hydroponics has emerged as a sustainable alternative, enabling year-round production, efficient resource utilization, and cultivation in non-arable areas. This study compares the growth performance, fruit quality and production costs of tomato grown hydroponically and in a in-ground system. For the inground system, there is a total of 20 tomato plants per plot, each of size 10 × 10ft, with three replications arranged in a completely randomized design. For hydroponic system, 30 tomato plants will be grown on bags filled with three different substrates (coconut coir, perlite, and pro mix), and bags were randomly placed inside one tunnel of size 32ft × 8ft, with three replications. Two indeterminate tomato varieties Early Girl Hybrid and Fourth of July Hybrid will be grown one after another using open drip hydroponic system and in in-ground system. Throughout the experiment, morphological traits such as plant height, leaf number, leaf area, relative chlorophyll content, fresh and dry biomass, stem diameter, and overall plant yield will be evaluated. Phytochemical parameters including total soluble solids, titratable acidity, lycopene content, phenolic compounds, and vitamin C levels will be assessed, economic parameters such as cost of labor, input supplies and net profit will be analyzed, and consumers acceptance testing by sensory analysis will be performed. This research aims to provide insights into the economic and agronomic viability of hydroponics compared to in-ground production. It is hypothesized that hydroponically grown tomatoes will exhibit comparable or superior nutritional quality while offering advantages such as higher yields, uniform fruit size, economically and sustainable sound production. The findings will support stakeholders in selecting optimal cultivation systems to enhance food production efficiency in response to global agricultural challenges.
Speakers
MJ

Mona Joshi

Kentucky State University
Co-authors
JH

James Howard

Kentucky State University
NA
MC

Milon chowdhury

Kentucky State University
SD

Siddhartha Dasgupta

Kentucky State University
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Marketing and Economics

12:15pm CDT

MKEC - Comparing Production And Market Acceptance Of Salad Greens And Cruciferous Vegetables Grown In Hydroponics Versus Tradit
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Hydroponic farming has gained increasing attention as a sustainable alternative to conventional soil-based agriculture, offering efficient resource utilization and enhanced crop production. This study evaluates the agronomic performance, economic viability, and market acceptance of hydroponic versus traditional in-ground cultivation for salad greens and cruciferous vegetables. The research examines key factors including crop yield, nutrient uptake efficiency, water consumption, production costs, and consumer preferences. Growth parameters such as biomass accumulation, nutrient absorption rates, and plant health indicators were assessed to compare the effectiveness of each system. Economic analysis includes production expenditures, labor requirements, and potential profitability, while market acceptance is evaluated through consumer surveys and purchasing trends. Results indicate that hydroponic systems demonstrate advantages in resource efficiency, faster growth rates, and potential for year-round production, making them a viable option for beginning and limited-resource farmers. However, challenges such as initial investment costs and market adaptation remain significant barriers to widespread adoption. Consumer perception studies reveal varying levels of acceptance based on factors such as taste, texture, and sustainability awareness. This research provides crucial insights into optimizing hydroponic farming strategies to enhance productivity, economic sustainability, and consumer engagement. Findings contribute to the broader discourse on sustainable agriculture, addressing the challenges faced by emerging farmers and exploring innovative solutions for improving food security and environmental stewardship. The study underscores the importance of integrating market-driven approaches with technological advancements to support the transition towards efficient and economically viable hydroponic production systems.
Speakers
AA

Amer Adawi

Kentucky State University
Co-authors
SD

Siddhartha Dasgupta

Kentucky State University
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Marketing and Economics

12:15pm CDT

MKEC - Consumer Willingness to Pay and Sensory Evaluation of Organic and Conventional Strawberries Grown in a Substrate-Based Tabletop Hydroponic System
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
The consumer demand has largely promoted organic strawberry production in the U.S. over the past two decades. The farm gate value of organic strawberries increased from $80 million in 2008 to $300 million in 2019, and organic strawberries accounted for about 25% of the 2022 U.S. strawberry sales. While Florida represents a smaller share than California, its organic acreage grew from less than 20 acres in 2008 to over 600 acres in 2019. Recent advances in agricultural technology have played an important role in improving strawberry yields and quality. The tabletop hydroponic system is among the new systems introduced to strawberry production with the aim of reducing labor requirements, enhancing input efficiency, and improving disease, pest, and weed management. However, no study has examined its impact on strawberry fruit regarding sensory attributes and consumer attitudes toward such a system for organic and conventional strawberry production. This study was conducted to assess consumers’ willingness to pay (WTP) for conventional and organic strawberries harvested from the tabletop hydroponic system under two scenarios, i.e., based on the blind sensory evaluation vs. after revealing the production method information (organic or conventional and tabletop hydroponic system). The consumer sensory evaluation was conducted at the University of Florida campus, with 61 participants. Participants first blind tasted four strawberry samples, including two organically grown and the other two conventionally produced, all from a tabletop growing system established for an on-farm trial. They evaluated sensory attributes using a just-about-right (JAR) scale and a hedonic scale, and reported their WTP before and after tasting the strawberry samples. Subsequently, participants were informed about organic and tabletop production methods used to produce the strawberries and asked to re-evaluate their WTP. Results indicate that consumers had a higher WTP for organic strawberries than their conventional counterparts, both before and after tasting. After being informed about which samples were organic, 28% of participants reported no additional WTP, 47% were willing to pay up to $1 more, and 22% were willing to pay $1 to $2 more, with an average premium of $0.79. These findings remained consistent after controlling for sensory attributes and demographics. When knowing the berries were produced in a tabletop system, the average WTP for organic strawberries increased by $0.73, while it increased by $0.56 for conventional strawberries. In addition, participants tended to associate organic strawberry samples with higher sensory evaluation scores and link lower sensory scores to conventional strawberry samples.
Speakers
XZ

Xin Zhao

University of Florida
Co-authors
CS

Charles Sims

University of Florida
NA
CH

Chenxi Hu

University of Florida
NA
WE

Wael Elwakil

Extension Agent II, University of Florida
YJ

Yifan Jing

University of Florida
NA
ZG

Zhifeng Gao

University of Florida
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Marketing and Economics

12:15pm CDT

MKEC - Decoding Consumer Preferences: The Impact of Wine Label Design and Packaging
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
This research investigates consumer perceptions and attitudes towards disease-resistant wine varieties, focusing on labeling, communication strategies, and barriers to adoption. Utilizing qualitative data from 500 participants, the study identifies key motivations for wine purchasing using the combination of latent Dirichlet allocation and thematic analysis. The findings reveal that consumers generally welcome the concept of disease-resistant wines, yet express concerns about terminology and the potential impact on wine quality. Effective communication strategies, emphasizing positive attributes and transparent information on labels, are crucial for fostering consumer trust. Additionally, barriers such as choice overload and fear of unfamiliar products highlight the need for targeted marketing initiatives. This study provides valuable insights for marketers in the wine industry, facilitating the introduction of sustainable wine varieties in a competitive marketplace.
Speakers
CY

Chengyan Yue

University of Minnesota-Twin Cities (Minneapolis, MN)
Co-authors
GS

Garrett Steede

University of Minnesota
NA
KG

Karina Gallardo

Washington State University
NA
MC

Matthew Clark

University of Minnesota
NA
UP

Uma Parasuram

University of Minnesota
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Marketing and Economics
  • Subject Marketing and Economics
  • Poster # csv
  • Funding Option SCRI funded all or part of the research associated with this abstract

12:15pm CDT

O/LT - Characterizing Phenotypic Variation in Ratibida columnifera Through Field and Greenhouse Studies
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Characterizing phenotypic variation in Ratibida columnifera (Nutt.) Woot.
Speakers Co-authors
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Ornamentals/Landscape and Turf

12:15pm CDT

O/LT - Evaluating Drought Responses in Four Native Ornamental Species from High-Elevation Desert Regions
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Due to low annual precipitation and high elevation, many residential areas in the Intermountain West region are classified as high-elevation deserts, necessitating the incorporation of drought-tolerant plants in residential landscapes. However, due to the short growing season and low winter temperatures, many commercial water-wise plants often fail to thrive in this region. Native plants from the Intermountain West could serve as low-water-use landscaping alternatives, but their drought tolerance remains unclear. The objectives of this study were to determine the effects of decreased medium volumetric water contents on the plant growth and physiology of four native ornamental plant species, including Asclepias speciosa (showy milkweed), Hedysarum boreale (Utah sweetvetch), Penstemon eriantherus (fuzzytongue penstemon), and Penstemon grandiflorus (shell-leaf beardtongue), in a greenhouse. Twenty-four plants of each species were randomly assigned to an automated irrigation system, and the substrate volumetric water content was maintained at 0.40 (control) or 0.20 m3⋅m−3 (drought) for 50 days. The decrease in substrate volumetric water content resulted in increased proportion of leaves visibly wilted across the four plant species and also impaired their visual quality. However, H. boreale, P. eriantherus, and P. grandiflorus receiving drought treatment where the substrate volumetric water content was 0.20 m³·m⁻³ still showed acceptable visual quality, while A. speciosa exhibited an unacceptable visual quality. However, the reduction in substrate volumetric water content from 0.40 to 0.20 m³·m⁻³ decreased relative plant growth index of A. speciosa and H. boreale by 19% and 17%, respectively, at the termination of the experiment. In addition, the total leaf area of H. boreale, P. eriantherus, and P. grandiflorus decreased from the control to the drought treatment, while the drought treatments reduced the dry weight of leaves of A. speciosa, H. boreale, and P. grandiflorus. To acclimate to the drought stress, A. speciosa and H. boreale modified their specific leaf area. In addition, H. boreale also increased its root to shoot ratio to adapt to water stress. Drought stress impaired the visual quality and growth of all four high-elevation desert ornamental species in this research. Penstemon eriantherus and P. grandiflorus maintained better visual quality and growth, making them more suitable for low water-use landscaping. Asclepias speciosa showed greater sensitivity to reduced water content despite its morphological adaptations to drought.
Speakers
DC

David Claypool

University of Wyoming
Co-authors
JC

Ji-Jhong Chen

University of Wyoming
RG

Robert Greenwald

Eastern Wyoming College
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Ornamentals/Landscape and Turf
  • Subject Ornamentals Landscape and Turf
  • Poster # csv
  • Funding Source University of Wyoming University of Wyoming College of Agriculture, Life Sciences, & Natural Resources, Office of Research and Economic Development Designated, Department of Plant Sciences. United States Department of Agriculture National Institute of Food and Agriculture (NIFA) Plant Sciences Hatch Funds (WYO-631-22)

12:15pm CDT

O/LT - Evaluating the Suitability of Bermudagrass and Zoysiagrass for Golf Tee Use in the Chicago Area
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Warm-season (C₄) turfgrass species such as bermudagrass (Cynodon spp.) and zoysiagrass (Zoysia spp.) exhibit superior recuperative potential compared to cool-season species. Excessive divot injury on cool-season turfgrass tees during peak golf play in summer can be problematic in Chicago, IL. Newer bermudagrasses and zoysiagrass are adaptable to northern climates, given improved cold tolerance. Improved cold tolerance and recuperative potential of newer bermudagrass and zoysiagrass suggest their possible use as a golf tee surface in temperate regions. This study evaluated newer cold-tolerant warm-season cultivars for use on golf course tees and was conducted in Lemont, IL. The RCBD study utilized eight treatments with five replications. Treatments included four bermudagrass cultivars (Tahoma 31, OKC3920, OSU15x9, OSU1629) and four zoysiagrass cultivars (Meyer, Innovation, DALZ1701, DALZ1808). Area Under the Progress Curve (AUPC) was used to integrate seasonal performance across timepoints. Bermudagrass entries Tahoma 31, OKC3920, and OSU1629 demonstrated significantly superior establishment rates, lateral spread, and turfgrass cover. In 2024, average turfgrass cover for the four bermudagrasses was 60% versus 21.1% for the four zoysiagrasses. All bermudagrasses retained acceptable green color ratings (≥6) by mid-October, with OSU15x9 showing extended fall green color retention. Meyer and DALZ1808 zoysiagrass were the earliest to break dormancy on Apr 29, 2025. Continued evaluation of cold-tolerant warm-season turfgrasses for winter survival is necessary prior to their use in northern climates.
Speakers
SS

Shehbaz Singh

Manager of Turfgrass research, Chicago District Golf Association
Co-authors
DS

Derek Settle

Chicago District Golf Association
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

O/LT - Influence of mulch application and planting depth on growth of Magnolia grandiflora 'Kay Parris'.
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
The effects of planting trees at improper depths have been clearly identified for many species. Improper planting often leads to reduced growth, reduced aesthetic value and increased mortality. Magnolia grandiflora is an evergreen species native to coastal states from North Carolina, south to Florida and west to Texas. The cultivar 'Kay Parris' is gaining popularity and is widely cultivated in the southeastern U.S. for its compact form and beauty in the landscape. On 18 December, 2023, asexually propagated 'Kay Parris' magnolia in 8.7L containers were transplanted to a field in Nacogdoches, TX. Plants were randomly assigned one of four planting depths (crown 5 cm above grade, crown planted at grade, crown 5 cm below grade and crown planted 10 cm below grade) and one of two mulching treatments (7.6cm of mulch applied or no mulch). Plants were randomly arranged in a modified complete block design comprised of 3 blocks with 2 plants of each treatment combination in a block. Dimensions of each plant (including height and width in two directions) were recorded at planting and ornamental ratings were assigned. After one year of establishment, the only statistically significant difference among the treatments was that those plants that received a mulch application grew taller than those with no mulch by over 7cm. No other statistical differences appeared in year one of the study. Year two of the study is ongoing.
Speakers
AK

Andrew King

Texas A&M AgriLife
Co-authors
DC

David Creech

SFA Gardens
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

O/LT - Public Perception of Diverse Seed Mixes for Urban Landscape Use
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
High diversity seed mixes have potential for providing ecological benefits including improvement of soil health, support of pollinator populations, and providing low maintenance landscaping. The focus of this research is to evaluate perception of the general public on the aesthetic value and desirability of diverse seed mixes in various settings. An online survey was deployed which included imagery of diverse seed mixes and conventional landscapes. Respondents were asked to rate desirability of the use of diverse seed mixes in various potential areas including home gardens, low traffic public spaces, and vacant lots. Respondents were also asked to evaluate the perceived ecological services associated with diverse seed mixes. Of respondents, 42 percent found diverse seed mix plantings appealing, compared to 52 percent who expressed that a conventional landscape was appealing. Greatest support for application of diverse seed mixes was for use in private gardens, vacant lots, and community garden spaces. Among respondents, enhancing appearance and support for pollinator populations were rates of highest importance in terms of landscaping benefits.
Speakers
MB

Molly Boyd

University of Nebraska-Lincoln
NA
Co-authors
CE

Christian Elowsky

University of Nebraska-Lincoln
NA
LL

Louise Lynch-O'Brien

University of Nebraska-Lincoln
NA
RS

Ronald Stephenson

Plant Sciences Hall
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

O/LT - Responses of Ornamental Groundcovers and Turfgrass Species to Drought Stress
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Water use in the landscape has become a more prevalent issue as the demand increases for more residential and commercial areas to be irrigated. This increase in water use indicates a need for drought tolerant plant species. Ornamental ground covers may provide an alternative to traditional turfgrass and provide water savings. Several studies have evaluated drought tolerance of turfgrass species, including Poa pratensis and Festuca arundinacea. Ground cover species, however, have less research available regarding drought tolerance. In this study, we analyzed six ground cover species (Dianthus caryophyllus, Lysimachia nummularia, Phlox subulata ‘Emerald Blue’, Sedum album, Stachys byzantina, and Vinca major) and three turfgrass species (Bouteloua dactyloides, Festuca arundinacea, and Poa pratensis) to evaluate their performance during drought and recovery. We conducted two studies to simulate drought-like conditions in a greenhouse at Kansas State University from April 2024 to November 2024 and May 2024 to January 2025. During the dry downs, plants were not watered until they reached a quality rating of one (1-9 scale, 1=dead/dormant and 9=best quality). Following the dry downs, normal watering resumed for 60 d to simulate a recovery period. Data collected three times per week during the drought period included quality rating (1-9), pot weight (g), and volumetric water content (θv); and one time per week we collected leaf water potential (ΨLeaf) and stomatal conductance (gs) readings. Results show S. album performed the best lasting an average of 157 d until reaching a quality rating of one. S. byzantina, B. dactyloides, V. major, D. Caryophyllus also performed well averaging 92, 51, 46, and 40 d, respectively, to a quality of one. P. subulate, P. pratensis, F. arundinacea, and L. nummularia performed the worst, averaging 35 d to decline to a quality rating of one. Following the dry down, the 60 d recovery period showed the following species recovered: B. dactyloides [58% Percent Green Cover (PGC)], F. arundinacea (45% PGC), L. nummularia (14% PGC), S. album (11% PGC), P. pratensis (10% PGC), and V. major (5% PGC). None of the other species recovered through the recovery period. Results indicate S. album, B. dactyloides, and V.major may perform the best in landscapes experiencing drought. F. arundinacea, L. nummularia, and P. pratensis may be good options as well if rain events or irrigation resumes following a dry or dormancy period.
Speakers
JC

Jacob Cody Domenghini

Assistant Professor, Kansas State University
Co-authors
LD

Levi Dreiling

Kansas State University
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

O/LT - The American Rose Trials for Sustainability® (A.R.T.S.®) Program Announces Fourteen Winning Roses for 2026
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
The American Rose Trials for Sustainability® (A.R.T.S.®) program trials roses throughout the US and is in its ninth year of announcing winning roses. Roses are evaluated monthly during the growing season for floral attributes (42.5% of score), foliar health and quality (45% of score), and growth habit (12.5% of score). Over the two-year trial, roses are grown using minimal inputs (i.e. no pesticides, no deadheading, moderate irrigation, etc.). Entries that score equal to or higher than the regional average of the control cultivars (Double Knock Out® [‘RADtko’] and Sunrise Sunset™ [‘BAIset’]) and have >50% survival by the end of the trial period, earn Local Artist awards based on Köppen climate region. Roses winning in four or more regions earn the added distinction of the Master Rose award. The 2026 award winning roses were trialed in 2023 and 2024. Six US Köppen climate regions were represented (Cfa, Cfb, Csa, Dfa, Dfb, and H) . Three roses won 2026 A.R.T.S.® Master Rose awards: Dessert First™ ('DD987-1'; Csa, Dfa, Dfb, and H), Ruby Red™ ('MEIzilena'; Csa, Dfa, Dfb, and H), and Blushing Drift® ('MEIfranjin'; Cfa, Cfb, Dfa, Dfb, and H). Eleven roses won 2026 A.R.T.S.® Local Artist awards: Celeste BlueSky™ ('P2046L'; Csa and Dfa), Easy Spirit™ ('WEKmereadoit'; Csa and H), Elizabeth® (AUSMajesty'; Cfa and Csa), Julie Andrews ('DELfluros'; Dfa), Maurice Utrillo™ ('DELstavo'; Csa), Patriot Dream™ ('BAIdre'; Dfa), Reminiscent® Coral ('BOZfra221'; Dfa), Ringo® Double Pink ('CHEWDelight', Csa abd Dfa), True Confidence™ ('FS118T'; Dfa), True Crush™ ('ESR015T'; Dfb), and White Lies™ ('POT3', H). Performance data will be provided by region for the two control and fourteen winning rose cultivars.
Speakers
avatar for Jeff Wilson

Jeff Wilson

Mississippi State University Extension
Co-authors
AB

Ashley Barkow

Green Bay Botanical Garden
NA
CS

Chris Schlenker

South Dakota State University
NA
CK

Crystal Kim

Red Butte Gardens-University of Utah
NA
DZ

David Zlesak

University of Wisconsin-River Falls
NA
GH

Gaye Hammond

Houston Rose Society
NA
GP

Gregory Pongetti

Arboretum and Botanical Garden at Cal State Fullerton
NA
JS

Jeff S. Kuehny

LSU AgCenter Botanic Gardens at Burden
NA
KM

Kristine M. Lang

South Dakota State University
MK

Mark Konlock

Green Bay Botanical Garden
NA
PD

Patrick Dahl

Chicago Botanic Garden
NA
RB

Rachel Burlington

Portland Parks
NA
RN

Randy Nelson

University of Minnesota-Crookston
NA
RH

Richard Hawke

Chicago Botanic Garden
NA
SG

Steve George

Texas A
NA
SW

Susan Worthey

Mississippi State University
NA
WE

Wanda Ellis

LSU AgCenter Botanic Gardens at Burden
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

O/LT - Transitioning bentgrass to subsurface drip irrigation during establishment in sand-based systems
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Establishment of bentgrass (Agrostis stolonifera) from seed in sand-based systems using subsurface drip irrigation (SDI) presents a challenge due to limited capillary rise of water from the drip emitter. An experiment was designed to evaluate bentgrass seed irrigated overhead at establishment and then transitioned to SDI once roots reach specific depths. Treatments consisted of a control (overhead irrigation only) and SDI irrigated overhead till roots reached 0, 5, 10 and 15 cm depth. Nineteen-liter (5 gallon) buckets (lysimeters) were filled with 5 cm of pea-gravel and then 30 cm mix of sand 90% and peatmoss 10%. Each bucket had a single drip emitter (gallon/hour) in the center of the bucket at a 15 cm depth. Data consisted of days to reach transition depth, percent cover, soil moisture and nitrogen. The SDI at 0 cm rooting depth treatment never established any bentgrass as irrigation from the emitter never reached the surface. The 5, 10 and 15 cm SDI treatments transition from overhead to drip 18, 27 and 38 days after seeding, respectively. The percent cover was 100, 82, 85 and 95% for the control 5, 10 and 15 cm treatments, respectively at 90 days after seeding. Volumetric water content was highest in the control and lower in the SDI treatments regardless of treatment. The SDI irrigation treatments developed tissue desiccation on the perimeter of the bucket after transitioning as the lateral capillary movement of water failed to reach the width of the bucket. Further research is needed to evaluate SDI spacing during transitioning that produce a high quality turfgrass in sand-based systems.
Speakers
MM

Michael Maurer

Stephen F. Austin State University
Co-authors
AS

Aakriti Sharma

Stephen F. Austin State University
NA
LK

Luke Keene

Stephen F. Austin State University
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

O/LT - Updating Fertilizer Best Management Practices for Sod Producers in Florida
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
This project is focused on improving nutrient management recommendations for sod producers as part of Florida’s best management practices (BMPs) program. The goal of this project is to evaluate nitrogen (N) fertilizer rate recommendations for Florida sod growers to achieve yield goals (shorten time to harvest) without negatively impacting water quality. The project was initiated in October 2023 and was conducted at four sod farms across the state and at the West Florida Research and Education Center (WFREC). At each farm, the project was conducted on two grass species. The N fertilizer (70% soluble plus 30% slow release) rates included 0, 120, 190, 260, and 330 lbs N/acre/year, which includes the range of current University of Florida, Institute of Food and Agricultural Sciences (UF/IFAS) recommendations. The annual N rate was split into monthly applications throughout the growing season. At the WFREC location, N applications were also being combined with phosphorus (P) fertilizer applications at 0 and 44 lbs P/acre/year to determine both N and P leaching. The treatments were arranged in a randomized complete block design with four replications. All other normal agronomic practices (irrigation, pesticides, etc.) were followed. Data collection included monthly visual turfgrass quality ratings, visual estimated percent cover, percent green cover via digital image analysis, and normalized difference vegetation index (NDVI). Soil cores and tissue samples were collected at the beginning and end of each growing season to determine soil chemical properties and nutrient concentrations. At harvest, sod samples were also collected to measure nutrient exports from fields. At WFREC, leachate was collected weekly and as needed based on rainfall events. Leachate volumes were measured at each water sampling event and subsamples from the leachate were analyzed for N and P concentrations. Preliminary results indicate that there are treatment differences in percent cover for all grasses. When grass species was combined across sod farms, the N rates of 260 and 330 lbs N/acre/year increased St. Augustinegrass [Stenotaphrum secundatum (Walter) Kuntze] percent cover. Additionally, the yearly rate of 330 lbs N/acre resulted in the greatest bermudagrass percent cover [Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt-Davy]. This experiment is being repeated, and additional analysis will occur after the second harvest cycle has been completed.
Speakers
VA

VICTOR ABARCA

University of Florida
Co-authors
AL

Alex Lindsey

University of Florida
BU

Bryan Unruh

University of Florida
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

PGR - Asynchrony in blueberries: use of ABA and MeJas to promote earlier coloration under in-vitro conditions
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Blueberries (Vaccinium spp.) exhibit a high degree of asynchrony during flowering, fruit set, and ripening. Fruit pigmentation is highly dependent on direct solar radiation, resulting in significant differences between sun-exposed and shaded berries within the same cluster. This study evaluated the effect of two plant growth regulators (PGRs), abscisic acid (ABA) and methyl jasmonate (MeJas), on fruit coloration and firmness under in vitro conditions. Clusters from two cultivars, ‘Duke’ and ‘Brigitta’, were collected at the green berry stage, two weeks before the expected start of commercial harvest in the 2024–25 season. Half of the clusters were taken from the outer canopy (sun-exposed) and half from the inner canopy (shaded). In the laboratory, clusters were placed in Falcon tubes with their twigs immersed in one of six solutions: (1) water (Control 1), (2) sucrose (Control 2), (3) ABA 500 ppm sucrose, (4) ABA 1000 ppm sucrose, (5) MeJas 0.1 mM sucrose, and (6) MeJas 1 mM sucrose. The samples were maintained in a growth chamber under controlled temperature (22 °C) and photoperiod (16 h light at 70 µmol/m²/sec). Fruit were evaluated at 3, 6, and 9 days post-treatment for coloration (percentage of blue fruit) and firmness (g mm⁻¹). ABA treatments significantly accelerated fruit coloration in both cultivars. In ‘Duke’, ABA-500 and ABA-1000 reached 80% and 100% blue fruit by day 6, respectively, compared to
Speakers
CM

Claudia Moggia

Universidad de Talca
Co-authors
CF

Carlos Figueroa

Universidad de Talca
NA
DS

Daniel Sepulveda

Universidad de Talca
NA
FL

Felipe Laurie

Universidad de Talca
NA
GA

Gustavo A. Lobos

Universidad de Talca
MV

Marcelo Valdes

Universidad de Talca
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Growth Regulation

12:15pm CDT

PGR - Managing Cider Apple Yield and Quality through Crop Load Management
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Many traditional European cider cultivars are known to have biennial bearing tendencies and growers have reported that conventional thinners such as 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (6-BA) are ineffective. The goal of this two-year study is to determine the efficacy of plant growth regulators (PGRs) on traditional European cider cultivars in reducing biennial bearing and improving juice and cider quality, while also maintaining sufficient yield to ensure profitability. The chemical thinners used in this study were applied with a backpack sprayer and consisted of the lowest and highest labelled rates of NAA (PoMaxa; Valent BioSciences LLC, Libertyville, IL) and 6-BA (MaxCel; Valent BioSciences LLC, Libertyville, IL) mixed with 1-naphthyl methylcarbamate (Carbaryl; Sevin SL; Loveland Products Inc, Greeley, CO). The chemical thinners were applied to the high-tannin cider cultivars, ‘Binet Rouge’, ‘Chisel Jersey’, ‘Dabinett’, ‘Harry Masters Jersey’, and ‘Porter’s Perfection’. The experiment was conducted at the Cornell research orchards in Ithaca and Lansing, NY, as well as commercial orchards in Walden, Lyndonville, and Trumansburg, NY. Each site was a fully replicated (n = 5) randomized complete block design. Preliminary data found that chemical thinning reduced crop load between 24% and 62% (measured as the total number of fruit per tree) and increased individual fruit weight (measured in grams) between 24% and 70%. Additionally, chemical thinning increased soluble solids concentration in the juice (measured in degrees Brix) between 6% and 29% and increased titratable acidity (measured in grams/liter) between 9% and 29%. These results demonstrate that PGRs can be effectively used to manage crop load for European cider cultivars. A second field season is planned for 2025.
Speakers
OB

Oksana Bihun

Cornell University
Co-authors
GP

Gregory Peck

Cornell University
MB

Michael Brown

Cornell University
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Growth Regulation
  • Subject Plant Growth Regulation
  • Poster # csv
  • Funding Source New York State Department of Agriculture and Markets and the USDA National Institute of Food and Agriculture

12:15pm CDT

PGR - Modification of core phosphorylation in photosystem II assembly using chloroplast transformation.
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Photosynthesis, the process by which light is converted into chemical energy, occurs in chloroplasts and involves two photosystems (PS II and PS I) connected by the cytochrome b6f complex. PS II is responsible for water splitting in oxygenic photosynthesis and is composed of a dimeric core and peripheral antennae. These antennae regulate excitation pressure under varying light conditions, preventing damage to PS II. In high-light environments, dissociation of the antennae is crucial for optimizing light harvesting and enabling self-repair of PS II. Phosphorylation of core proteins, particularly at the Thr2 residue in PsbA (D1) and PsbD (D2), plays a key role in antenna regulation and PS II repair. However, the mechanisms underlying phosphorylation-dependent disassembly of PS II are not well understood. In this study, we generated mutated PsbA and PsbD genes with alanine substitutions at the Thr2 phosphorylation site. These mutated genes were introduced into the chloroplast genome via particle bombardment, and homologous recombination was used to replace the native genes. Characterizing plants with these mutations will provide insight into the role of phosphorylation in the disassembly of PS II, a process crucial for light-harvesting efficiency and repair. This work aims to enhance our understanding of photosynthetic regulation and inform strategies for improving crop efficiency.
Speakers Co-authors
SP

Sujith Puthiyaveetil

Purdue University
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Growth Regulation
  • Subject Plant Growth Regulation
  • Poster # csv
  • Funding Source This work was supported in part by a DOE-Office of Science Grant to SP & AD; Texas A&M AgriLife Hatch Project #TEX0-9950-0, and startup funds from Texas A&M AgriLife Research and Texas A&M University to AD.

12:15pm CDT

PGR - Response of Gillenia stipulata to Ethephon and Far-red Light
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Gillenia stipulata, commonly known as Wester Indian Physic, is an ornamental herbaceous perennial in the Rosaceae family native to the Midwest and Eastern North America. Ethephon has been previously shown to affect plant growth, development and early flowering in apple seedlings. In the present study, interaction of ethephon and light quality on G. stipulata plant growth and development was examined. Plants were grown from seed purchased from Prairie Moon Nursery (Winona, MN) and when 4-5 leaves had been initiated, seedlings were treated with 0 (Control), 500 ppm, 1000 ppm, or 2000 ppm Florel (Monterey Lan
Speakers
SM

Sonja Maki

University of Wisconsin-River Falls
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

POM 1 - Effect of Cellulose Nanofiber (CNF) as a Potential Cryoprotectant to Reduce Spring Frost Damage in Pear and Apple Cultiv
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Ongoing climate change has resulted in unusually high winter temperatures and earlier spring warming, which have caused major fruit trees to bloom earlier than in previous years. This shift is increasing the risk of spring frost damage, emphasizing the urgent need for eco-friendly, sustainable and cost-effective protective strategies. Considering these, this present study, cellulose nanofiber (CNF), a nanomaterial extracted from plant cell wall cellulose from wood and pulp via mechanical (RE) and chemical (TEMPO) processes, was combined with sodium alginate (AG), a natural substance extracted from seaweed. It was applied as a coating material to provide thermal insulation and assess its effectiveness in protecting pear cultivars ‘Niitaka’ and ‘Wonhwang’ and apple cultivars ‘Hongro’ and ‘Fuji’. As frost damage was forecasted to occur on March 30, 2025, cryoprotectants were applied to apples on March 28, 2025, and pears on March 29, 2025. In pears, treatments were applied to ‘Niitaka’ and ‘Wonhwang’ trees in an orchard in Jinju, Gyeongsangnam-do, South Korea. Five treatments (control, 1.0% RE-CNF, 1.1% TEMPO-CNF, RE:AG=8:2, and TEMPO:AG=8:2) were applied to five trees per cultivar. Spraying was conducted once at the early flower cluster visibility stage (Stage 4) for ‘Niitaka’ and at the half-inch green stage (Stage 3) for ‘Wonhwang’. For apples, treatments were applied to 15 trees each of ‘Hongro’ and ‘Fuji’ cultivars located in an orchard in Hamyang-gun, Gyeongsangnam-do, South Korea. The flower bud stage for both ‘Hongro’ and ‘Fuji’ was at the half-inch green (Stage 4) when a single spray was applied. The study established that in ‘Niitaka’ pears the TEMPO:AG=8:2 treatment achieved the lowest pistil and stamen damage rates at 64% and 83%, respectively, reducing damage by 17% and 11% compared to the control. In ‘Wonhwang’ pears, the pistil damage rate was lowest as 11% with RE-CNF, and the combined pistil and stamen damage rate was lowest as 56% with TEMPO-CNF, showing reductions of 14% and 9% compared to the control. In both ‘Hongro’ and ‘Fuji’ apples, the combined pistil and stamen damage rate was 18% and 7%, respectively, with the TEMPO:AG = 8:2 treatment, showing 12% and 21% reductions compared to the control. In conclusion, the CNF mixtures revealed effective frost protection by significantly reducing frost damage rates in pears and apples. Thus, it is considered the most suitable material for developing cryoprotectants to mitigate spring frost damage in these major fruit crops.
Speakers
avatar for HYUNSUK SHIN

HYUNSUK SHIN

Gyeongsang National University
Co-authors
HJ

HYO JEONG KIM

Gyeongsang National University
NA
PM

PANDIYAN MUTHURAMALINGAM

Gyeongsang National University
NA
SP

SUJEONG PARK

Gyeongsang National University
NA
YP

YEONJU PARK

Gyeongsang National University
NA
YR

YERIM RYU

Gyeongsang National University
NA
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 2
  • Subject Pomology
  • Poster # csv
  • Funding Source The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was carried out with the support of the “Cooperative Research Program for Agricultural Science and Technology Development (Project No. RS-2025-02183070)” funded by the Rural Development Administration, Republic of Korea.
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

12:15pm CDT

POM 1 - Evaluation of Select Plant Growth Regulators on Improving Fruit Set in Apricot (Prunus armeniaca)
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Apricot (Prunus armeniaca L.) production in Texas is limited by inconsistent and overall poor yields due to spring frost and loss of hardiness in buds associated with erratic temperature swings in late winter/early spring. Several plant growth regulators (PGRs) were evaluated for improving yields. PGRs were applied in the fall and spring to assess their potential for mitigating floral bud injury, improving bud hardiness, enhancing fruit set, and improving fruit retention rates. Two-year-old ‘Wenatchee Moorpark’ trees on ‘KRYMSK-1’ rootstock, spaced at 1.33 by 3.33 meters were used with a randomized complete block design with five single-plant replications. Fall applications were made at approximately 50% natural leaf fall of Ethephon at rates of 100, 200, and 300 mg/L-1, Accede (ACC) at rates of 500, 1000, and 1500 mg/L-1, and ProTone (S-ABA) at rates of 500 and 1000 mg/L-1. Following defoliation of the trees, white latex paint was also applied as a treatment to the entire canopy and trunk of dormant trees. Spring treatments aimed at improving fruit set were applied during full bloom and included Promalin (GA4+7, 6-BA) at rates of 1.17 and 2.34 L/ha-1, and ReTain (AVG) at rates of 889 and 1,778 mg/L-1. Additionally, evaluations were conducted on combination treatments of fall and spring applications. These treatments included Ethephon+Promalin+Latex; Ethephon+ReTain+Latex; Accede+Promalin+Latex, and Accede+ReTain+Latex, which were applied at the highest concentrations for each product evaluated individually in this study. Differential Thermal Analysis (DTA) was carried out on the 15th and 16th of February 2025 using 5 floral buds per tree as sub-samples. LT50 found Accede at 1500 mg/L-1 significantly improved bud hardiness by 4.67°C relative to the control. Additionally, treatments of Accede+Latex, Accede+Promalin+Latex, and all three combination treatments of Ethephon delayed floral bud development relative to control. However, Ethephon produced significant gummosis and visible injuries, whereas Accede produced negligible gummosis. Fruit set, as assessed by counting fruit on 50.8cm lengths of standard sample shoots, was improved using the highest concentration of ReTain (AVG). The mean fruit number increased from 1.2 for the control, to 5.0 for AVG, at P= 0.0002, occurring after exposure to temperatures below -4°C on March 19th. The results from this and future years of this trial are anticipated to assist in improving the feasibility of commercial apricot production within Texas and similar environments.

Speakers Co-authors
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 2
  • Subject Pomology
  • Poster # csv
  • Funding Source USDA Specialty Crop Block Grant Program
  • Funding Option SCRI funded all or part of the research associated with this abstract

12:15pm CDT

POM 1 - Fruit Weights, Percent Seed, and Soluble Solids Content of Six Advanced Selections from Kentucky State University’s Pawp
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
The North American Pawpaw (Asimina triloba) is a tree fruit native to the Eastern U.S. which is being produced in commercial orchards across the U.S. and internationally, with an increasing market and interest. There are approximately 50 pawpaw cultivars available, but many of these varieties have low yields with fruit sizes below the threshold of 120 grams needed for commercial sale and processing. New high yielding cultivars with excellent fruit quality would further assist in the development of the pawpaw industry. Kentucky State University serves as the National Clonal Germplasm Repository for Pawpaw. Two goals of the Repository research efforts are germplasm acquisition and evaluation. The repository contains over 2000 accessions from 16 different states; additionally, both open pollinated seedlings from superior genotypes and crosses of superior selections have been incorporated into the repository collection. Fruit weights, percent seed by weight, and soluble solids content from six of KSU’s advanced selections (Hi 1-4, Hi 7-1, G4-25, G6-120, G9-109, and G9-111) were compared to the cultivars ‘KSU-Atwood’, ‘KSU-Chappell’, and ‘Ralph’s Whopper’. All advanced selections had average fruit weights over 120 grams with Hi 1-4 having the highest fruit weight (229.8 g). ‘KSU-Chappell’ had the lowest percent seed (4.7%), with Hi 1-4 having the second lowest (5.9%). Hi 1-4 had the highest soluble solids content at 25.9 Brix and all selections examined were above 21 Brix. Several selections including Hi 1-4 and Hi 7-1 show potential to be promising new releases and have been budded onto seedling rootstock for further evaluation.
Speakers
JL

Jeremy Lowe

Kentucky State University
Co-authors
KB

Kirie Broberg

Kentucky State University
NA
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
PB

Page Barker

Kentucky State University
NA
SC

Sheri Crabtree

Kentucky State University
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 2
  • Subject Pomology
  • Poster # csv
  • Funding Source Evans Allen

12:15pm CDT

POM 1 - Hawk238: A Unique Pawpaw Selection for Kentucky Production?
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
The North American pawpaw (Asimina triloba (L.) Dunal) is a native tree fruit that is in the early stages of commercial production. According to the 2022 Census of Agriculture, there are over 90 commercial pawpaw growers in Kentucky. Pawpaw fruit has fresh market appeal for farmers' markets, community-supported agriculture, and organic markets, as well as processing potential for frozen pulp production. There are approximately 50 pawpaw cultivars commercially available. Kentucky State University has already released three pawpaw cultivars: KSU-Atwood™ in 2009, KSU-Benson™ in 2016, and KSU-Chappell™ in 2018. However, additional high-quality cultivars are needed. Pawpaw varieties with fruit weights over 120 g per fruit are considered to have a large enough fruit size for commercial sale and processing. New high-yielding cultivars with excellent fruit quality would further assist in the development of the pawpaw industry. Kentucky State University serves as the National Clonal Germplasm Repository for Pawpaw. Two goals of the Repository’s research efforts are germplasm acquisition and evaluation. The selection Hawk238 was identified by a private grower as having a unique fruit type and promising characteristics as a potential new cultivar. In 2024, the fruit size and quality of the pawpaw cultivars Susquehanna and Shenandoah were compared to Hawk238. Fruit size was similar among ‘Susquehanna,’ ‘Shenandoah,’ and Hawk238, averaging 190 g, 155 g, and 154 g, respectively. The Brix of ripe fruit pulp was significantly lower in ‘Shenandoah’ (17.7 Brix) compared to ‘Susquehanna’ (25.4 Brix) and Hawk238 (24.3 Brix). The selection Hawk238 has a pleasing orange flesh with a mild banana-mango flavor and a large fruit size that would be excellent for processing. This selection, Hawk238, will be included in additional trials.
Speakers
avatar for Kirk Pomper

Kirk Pomper

Professor-College of Agriculture, Kentucky State University
Co-authors
JL

Jeremy Lowe

Kentucky State University
SC

Sheri Crabtree

Kentucky State University
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 2
  • Subject Pomology
  • Poster # csv
  • Funding Source USDA Evans Allen Capacity Funding

12:15pm CDT

POM 1 - Hi1-4 and Hi7-1: Yield Data on Two Promising New Selections from Kentucky State University’s Pawpaw Breeding Program
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
The North American pawpaw (Asimina triloba) is a tree fruit native to North America, and is being produced on a small scale commercially across the U.S. and internationally, with over 90 pawpaw growers in Kentucky alone. There are approximately 50 pawpaw cultivars available, but some of these varieties tend to be poor quality, low yielding, have small fruit size, or exhibit poor flavors. Kentucky State University’s pawpaw breeding program is striving to develop new improved pawpaw cultivars with good flavor and high yields. A pawpaw variety trial was established at the Kentucky State University Harold R. Benson Research and Demonstration Farm in Frankfort, KY, in 2017 consisting of two commercially available cultivars (‘KSU-Benson’ and ‘KSU-Chappell’) and two KSU advanced selections (Hi1-4 and Hi7-1). Data on number of fruit and clusters per tree and fruit weights were collected to compare advanced selections Hi1-4 and Hi7-1 to KSU-Benson and KSU-Chappell. The advanced selections Hi1-4 and Hi7-1 had large average fruit weights of 213 g (Hi1-4) and 167 g (Hi7-1). The cultivars KSU-Benson (179 g) and KSU-Chappell (234 g) also had large marketable fruit. Pawpaw fruit over 120 g are generally considered to be large enough for commercial sale and processing. Hi1-4 and Hi7-1 had an average yield of 12.8 kg and 12.0 kg per tree respectively in 2024, compared to KSU-Benson and KSU-Chappell with average yields of 16.9 kg and 27.2 kg respectively. Advanced selection Hi1-4 (1.7) and cultivar KSU-Benson (1.8) averaged under 2 fruit per cluster, while Hi7-1 (2.1) and KSU-Chappell (2.5) averaged slightly over 2 fruit per cluster. It is desirable for pawpaws to have fewer fruit per cluster and more single fruit, for ease of harvesting. Hi1-4 and Hi7-1 have large, flavorful fruit and high yields, and are planned to be released as new cultivars from the KYSU pawpaw breeding program in Fall 2025.
Speakers
SC

Sheri Crabtree

Horticulture Research and Extension Associate, Kentucky State University
Co-authors
JL

Jeremy Lowe

Kentucky State University
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 2
  • Subject Pomology
  • Poster # csv
  • Funding Source USDA Evans-Allen Research

12:15pm CDT

POM 1 - Influence of propagation method and irrigation level on coarse root system architecture of Prunus rootstocks
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Root system development in perennial crops is critical for the long-term survival of trees. Long lived coarse roots form the rigid structure that supports the dynamics of the absorptive fine root system. The woody root scaffold developed early in a tree’s life can shape lasting aspects of mature root system structure and function, including the extent of soil exploration. Research on early root scaffold development under different propagation and field management conditions has untapped potential for orchard system improvement. Rootstock propagation method significantly influences root system architecture (RSA). Seed propagation in Prunus species produces a vertically oriented taproot at germination, with lateral roots developing along this main axis. In contrast, clonally propagated rootstocks (e.g., cuttings, tissue culture) develop adventitious roots from a wound site or callus, often resulting in shallower, clustered root systems emerging from a concentrated point of origin. While clonal propagation exhibits trait uniformity and a high rate of initial root production, excessive root crowding and limited vertical distribution could lead to structural deformities, increased susceptibility to soil-borne disease, and reduced soil exploration for water and nutrients as trees mature. Studies have shown that a greater number of roots increases initial transplanting success, but a profusion of scaffold roots may result in competition for space, leading to permanent changes in RSA detrimental to long-term tree health. This study examines RSA traits (the spatial configuration of roots, including distribution, morphology, and topology) for coarse roots in commercial almond rootstock cultivars that were either seed or clonally propagated and subjected to full or deficit irrigation regimes. We evaluate how propagation method influences root angle (deep water access), scion growth (survival and vigor), root crowding (potential disease risk), and root distribution (soil exploration) in this 3-year common garden experiment. We found that rootstock significantly influenced traits like mean root diameter and convex hull (p < 0.05), suggesting genotypic differences in root vigor and extension into the soil profile. Propagation method did not significantly affect root angle after the first season, but prolonged exposure to deficit irrigation conditions in succeeding years will be important to understanding the adaptive capacity of these traits over time. As climate variability intensifies, understanding how rootstock propagation impacts RSA traits–particularly those relevant for accessing water stores, such as deeper root systems, elevated root vigor, and greater root length density at depth–will be essential for improving almond production in drought conditions, suboptimal soils, and other marginal environments.
Speakers
Wednesday July 30, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 2

12:15pm CDT

POM 1 - Phenological Modeling of Pollinizing Crabapple Cultivars (Malus spp.) and Commercial Apple Cultivars (Malus X domestica Borkh.) in Kentucky
Wednesday July 30, 2025 12:15pm - 1:30pm CDT
Apple (Malus X domestica Borkh.) cultivars require a pollinizer for cross-pollination to ensure fruit set when planted in contiguous blocks found in modern apple orchards. The overlap of bloom time between pollinizing crabapples (Malus spp.) and commercial cultivars is a critical component for adequate fruit set to occur. Bloom time and duration is correlated with temperature, suggesting this relationship could be influenced by climate change. Understanding the influence temperature has on bloom phenology of these pollinizing crabapples is important to ensure proper cultivar and pollinizer combinations are made by growers. 4 commercially available pollinizing crabapple cultivars (‘Indian Summer’, ‘Mt. Blanc™’, ‘Mt. Evereste™’, ‘Snow Drift’) were replicated 5 times in completely randomized design. In spring of 2024 and 2025, pollinizers were analyzed 1-2 times per week for developmental stages from silver tip to petal fall. These two years represented one of the warmest and then coldest springs on record in Kentucky, respectively. Commercial apple cultivars were also analyzed for comparison. The stage progression data for each pollinizer cultivar was modeled using growing degrees days (GDD) base 6.6°C. In both observed years, the first crabapple to bloom was ‘Indian Summer’ and the final crabapple to bloom was ‘Mt. Blanc™’. In the first year, ‘Indian Summer’ bloomed on Julian Day (JD) 88 at 166.4 GGD and in the second year bloomed on JD 92 at 179.8 GDD. 'Mt. Blanc™’ bloomed on JD 104 at 250.1 GDD and 244.9 GDD in years one and two, respectively. In year one, ‘Gold Rush’ began bloom on JD 97 at 199.4 GDD, which overlapped with ‘Mt. Evereste™’ and ‘Snow Drift’, whereas in the second year ‘Gold Rush’ began bloom on JD 93 at 179.4 GDD and stopped blooming on JD 108 at 279.8 GDD, which overlapped with ‘Mt. Evereste™’, ‘Snow Drift’, and ‘Mt. Blanc™’. There was little bloom overlap in 2024, with the earliest crabapples blooming earlier and more rapidly than the cultivars. In 2025, bloom overlap improved but little overlap occurred with more than two pollinizers at a time. These results show bloom overlap can be influenced by different temperature patterns during winter and spring, and pollinizer selection needs to be carefully considered. It can be concluded that using growing degree-days can help predict bloom time for pollinizers. In Kentucky, limited bloom overlap of apples and pollinizers could indicate a concern for northern apple growing regions as spring temperatures become more variable with climate change.
Speakers
ES

Elayna Stirn

University of Kentucky
Co-authors
BA

Brent Arnoldussen

University of Kentucky
NA
MW

Miranda Woodcock

University of Kentucky
Wednesday July 30, 2025 12:15pm - 1:30pm CDT
Empire AB

1:00pm CDT

OPB - Visual color of redbud flowers depends on key anthocyanins
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Redbud (Cercis spp.) trees provide colorful spring bloom in much of North America. Although most genotypes are green-leaved, some cultivars have been developed with yellow, red, or purple leaves. Depending on genotype, flowers can be white, pink, purple, or red. Previously we found that leaf petioles from red or purple flowered redbuds contain peonidin-3-glucoside, purple-flowered redbuds also contain malvidin-3-glucoside, red-flowered redbuds lack appreciable malvidin-3-glucoside, and white flower types lack either pigment. In the current study, we followed the anthocyanins present in the calyx, petal, and pedicel of flowers in purple, red, yellow and green leaved genotypes to determine pigment profile continuity and possible leaf color influence on pigments. A total of 30 cultivar/genotypes of redbud flowers were used, including C. chinensis, C. glabra, C. canadensis, and C. canadensis ssp. texensis, mexicana. Calyx, petal, and pedicel of 20 cultivars were also subjected to extraction of pigments with acidified methanol and identification of anthocyanins using HPLC and PDA. Overall, visual categorization of flowers as red or purple and flower components correlated with the presence (purple) or absence (red) of malvidin 3-glucoside. Purple flowers from red or purple-leaved redbuds had a more diverse anthocyanin profile and were 1.5 to 3 fold higher in total anthocyanin than those from green-leaved redbuds. Red flowers were dominant in peonidin-3,5-diglucoside. These studies demonstrate that multiple flower tissues will yield similar anthocyanin profiles in redbud flowers, and that the genetics affecting leaf color can also influence the amount of pigment in redbud flowers. Additionally, the redbud species and genotypes used in this study appear to exhibit a pattern of flower pigmentation controlled by synthesis of malvidin-3-glucoside and peonidin-3-glucoside.
Speakers Co-authors
GM

Guoying Ma

North Carolina State University
NA
HC

Hsuan Chen

North Carolina State University
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

OPB - ‘Sunshine’, ‘Lemondrop’, ‘Chico’, and ‘Sundrop’, Four New Ornamental Rhizoma Perennial Peanut Cultivars (Arachis glabrat
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Arachis glabrata is a drought resistant, low maintenance, pest resistant, nitrogen fixer, ornamental ground cover used in tropical and subtropical climates. Although popular in landscapes in the State of Florida for its attractive leaf color, low maintenance needs, orange flowers, and low mowing requirements, there is only one A. glabrata ornamental cultivar available in the market: ‘EcoTurf’. ‘EcoTurf’ is dual purpose cultivar (ornamental/hay) due to its low height (15-20 cm), compared to the many hay cultivars available. The University of Florida/IFAS Ornamental Perennial Peanut Breeding Program has been developing, trialing, and selecting lines with unique or improved ornamental characteristic, all developed by single rhizome descent at the UF/IFAS North Florida Research and Education Center. ‘Sunshine’ is a profuse bloomer with flowers almost double the size than ‘EcoTurf’, bright orange flowers, and waxy leaves. ‘Lemondrop’ is the only A. glabrata cultivar with yellow flowers, compact and dense foliage, and waxy leaves. ‘Chico’ has glaucous, dark-green leaves, and a dense and compact foliage. And ‘Sundrop’ with yellow-orange flowers, a long flowering period, and dark-green, glaucous, dense and compact foliage. All these new cultivars have a lower grower habit (10-15 cm) than the current industry standard ‘EcoTurf’. These new cultivars of Ornamental Rhizoma Perennial Peanut bring a new pallet of leaf types, flower size, flower color, and growth habit to the ornamental market, with great potential to be used in landscapes across the southeastern US.
Speakers
avatar for Leynar Leyton

Leynar Leyton

Assistant Professor, University of Florida
Co-authors
AB

Ann Blount

UF/IFAS North Florida Research and Education Center
NA
BU

Bryan Unruh

UF/IFAS West Florida Research and Education Center
NA
CM

Cheryl Mackowiak

UF/IFAS North Florida Research and Education Center
NA
GK

Gary Knox

UF/IFAS North Florida Research and Education Center
NA
KT

Kelly Thomas

Chicago Botanical Garden
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

ORGH 2 - Biofumigation Blast: Battling Soil-Borne Bacteria Organically
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Soil-borne bacterial pathogens pose a significant threat to organic onion production as there is a lack of effective pathogen control practices for both conventional and organic systems. Burkholderia cepacia (causal agent of sour skin) and B. gladioli pv. alliicola (causal agent of slippery skin) are prevalent throughout the onion growing regions of the U.S., including Georgia. Organic growers rely on cultural practices to combat soil-borne diseases, pests, and weeds. Plants in the Brassica family contain glucosinolates which undergo hydrolysis and produce isothiocyanate volatiles serving as a biofumigant. Biofumigation has shown to be successful at reducing diseases, but efficacy is dependent on pathogen, environmental conditions, cover crop biomass, and soil conditions. The objective of this study aims to determine biofumigation effect on soil-borne bacterial pathogens associated with Burkholderia spp. This study was conducted on organic certified land using B. juncea ‘Pacific Gold’ for mustards and A. cepa ‘Sweet Tule’ for short-day onion crop. Mustards were grown and terminated at flower initiation with biofumigation treatments including mustard incorporated into soil, mustard incorporated into soil and covered with polyethylene mulch, and a no mustard control. To evaluate effectiveness of biofumigation, measurements on weed seed germination and biomass, disease incidence/severity in bulbs, and onion yield were measured. Weed seed germination was evaluated before and after the incorporation of the mustards. Weed biomass was measured at multiple time points throughout the onion growing season. At harvest, onions were graded according to the USDA standards (Colossal, Jumbo, Medium, and Cull). Bulbs were evaluated for the presence of both diseases at harvest. Post-harvest evaluation of bulbs was also conducted. A 50 bulb sub-sample/plot/treatment was placed in storage (-2 °C, 70% RH) for a period of 90 days. After storage, bulbs were sliced, and disease incidence/severity was recorded. At mustard termination, dry biomass among all treatments averaged 4,540.5 lbs/acre. Eleven weeks after mustard incorporation, treatments with plastic mulch following mustard incorporation had significantly less weeds than the control treatments.
Speakers
JP

James Pulliam

University of Georgia
Co-authors
KC

Kate Cassity-Duffey

University of Georgia
TC

Timothy Coolong

University of Georgia
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 1

1:00pm CDT

ORGH 2 - Effect of water-soluble organic fertilizer on the growth and development of highwire cucumbers grown in controlled envir
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Organic produce sales have increased over time as many consumers are willing to pay a higher price for organically-grown produce compared to conventionally-grown. Previous research has shown that liquid organic fertilizers outperform substrate-incorporated organic fertilizers in containerized production with soilless substrates. Therefore, developing new production protocols with water soluble organic fertilizers may increase yield and provide easier nutrient management. The objective of the current study was to identify water-soluble organic fertilizer combinations for growth and development of cucumber, Cucumis sativus ‘Picolino F1’, grown in a 40:60 wood fiber : coir substrate for ten weeks. Five fertilizer treatments were: molasses-based Pre-Empt (PE) at either 120 or 170 mg.L-1 nitrogen (N) in combination with 100 mg.L-1 calcium (Ca) from either calcium chloride (CaCl2) or FoxFarm’s Cal-Mag. A synthetic fertilizer treatment (Hydro-Gro Vine; N: 4.40% - P: 13.00% - K: 34.00% and calcium nitrate) was included as a control. The Cal-Mag 170 mg.L-1 N treatment had a similar yield to the control. However, there was no significant difference between all organic treatments for growth parameters measured. Additionally, leaf tissue analyses revealed boron increased by 22% to 37% and manganese by 37% above the sufficiency range for most of the organic fertilizer treatments. Meanwhile, N and potassium (K) concentration dropped below the required range by 45% and 49%, respectively, in all organic treatments. Future experiments will identify sources of supplemental N and K with the goal of supplying sufficient levels of all macro and micronutrients using water soluble organic fertilizers.
Speakers
JM

JOANNA MOINE

The Ohio State University
Co-authors
AT

Anna Testen

USDA-ARS
NA
MK

Matthew Kleinhenz

The Ohio State University
NA
US

Uttara Samarakoon

The Ohio State University
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 1
  • Subject Organic
  • Poster # csv
  • Funding Source USDA-NIFA NCR-SARE

1:00pm CDT

ORGH 2 - Enhancing Mineralization Pattern of Organic Nutrient Sources Through Natural Amendments
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Organic materials such as meat and bone meal (MBM; rendering product) and treated manure products can be excellent fertilizers due to balanced availability of nutrients. In this study, we investigated the influence of natural amendments (NAs), specifically sulfur and neem oil, on nitrogen (N) mineralization dynamics from MBM and treated manure solids upto 63 days of incubation under controlled environment at 4 different time points (7th, 21st, 35th and 63rd days). Soil incubation experiments revealed distinct mineralization patterns between MBM and treated manure solids. MBM exhibited a faster N mineralization rate compared to treated manure solids. MBM treated with sulfur (25% of N) showed the highest net N mineralization rate (49.83%) as compared to untreated MBM (40.91%) by day 21. However, when MBM was amended with Neem oil (10% of N), the net N mineralization decreased significantly to 36.68% by day 21. There was no statistical difference observed between MBM, MBM with sulfur (25% of N), MBM with neem oil (10% of N) for net N mineralization at day 7. Similarly, treated manure solids with sulfur (25% N) non significantly reduced net N mineralization to 8.86% and 2.13% at 21 day and 7 days respectively. Further, we will be evaluating the effect of these treatments on soil biological health and C, N and P enzymatic activity. This comprehensive evaluation aims to provide insights into optimizing the use of natural amendments for strengthening nutrient management in organic agricultural systems.
Speakers
avatar for Ashok Sah

Ashok Sah

Clemson University
I am a Ph.D. student working on anaerobic soil disinfestation (ASD) technique, its application for nutrient and weed management in horticultural crops (watermelon).
Co-authors
BJ

Bhupinder Jatana

Clemson University
TG

Tom Grayson Sanders III

Clemson University
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 1
  • Subject Organic
  • Poster # csv
  • Funding Source USDA

1:00pm CDT

ORGH 2 - Role of Chlorella Application on Yield and Phytochemical Composition of Mustard Greens
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Role of Chlorella Application on Yield and Phytochemical Composition of Mustard Greens Sam Pratt1*, Shivani Kathi1, Steve Phillips2, and Justin Moss1 1Department of Horticulture and Landscape Architecture, Oklahoma State University, Stillwater, OK, 74078 2Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078 *Presenting author- Samatha.pratt@okstate.edu Organic nitrogen fertilizers have lower percentage of nitrogen than their chemical counterparts. Furthermore, nitrogen from organic fertilizers tend to be less readily available. Chlorella (Chlorella vulgaris) has been used as a biofertilizer in horticultural crop production in controlled environment production systems due to its role in nitrogen fixation. However, research gaps exist in the application of chlorella biofertilizer in open-field production systems. The goal of this research is to assess the impact of microalgae biofertilizers on crop productivity and nutritional quality of mustard green grown in open-field conditions under 100% nitrogen and nitrogen-deficit (50%) conditions. Treatments included chlorella applied through foliar spray, soil application, and a combination of foliar and soil arranged randomized complete block design in four blocks. Data was collected on yield (i.e., marketable and total yield) and nutrient composition of leafy greens (chlorophyll, carotenoids, and mineral nutrients composition). Soil application of chlorella under 100% fertilizer rate was more effective application method for the overall impact on yield. The crops that received lower amount of fertilizer and chlorella treatment had the lowest yield. Future research should focus on addition of Chlorella in different crop production systems with different organic fertilizers for additional nitrogen sources in low chemical input and organic based production systems.
Speakers
SP

Samantha Pratt

Oklahoma State University
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 1

1:00pm CDT

ORGH 2 - Root to Shoot: Strawberry Cultivar Growth Responses to Anaerobic Soil Disinfestation
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
With the goal of elucidating the influence of anaerobic soil disinfestation (ASD) on organic strawberry production, plant growth responses of strawberry cultivars to ASD treatments under Florida sandy soil conditions were evaluated in this study across two locations. In both locations (Balm and Citra), a split-plot design with four replications was used in the field trial with the ASD treatment as the whole plot factor and the strawberry cultivar as the subplot factor. The three-week ASD treatments consisted of 13.9 m³/ha molasses combined with Everlizer 3-3-3 (a heat-processed poultry manure organic fertilizer) at either 9.5 (ASD1) or 14.25 Mg/ha (ASD2) in comparison with the no ASD control, while the strawberry cultivars included ‘Ember’, ‘Encore’, ‘Florida Brilliance’, ‘Florida Medallion’, ‘Florida Pearl’, and ‘Florida127’. Destructive sampling was conducted at mid-season and final harvest. Significant effects of ASD treatment and strawberry cultivar were observed for most measured traits, with no significant interaction observed. Across locations and plant stages, ASD treatments significantly enhanced shoot fresh weight and dry weight, crown diameter and number, and leaf number relative to the no ASD control. At Balm, ASD2 increased shoot fresh weight and dry weight by 21% and 9%, respectively, compared with the no ASD control at final harvest. Similar trends were observed at Citra, where ASD2 resulted in higher shoot fresh weight and dry weight by 29% and 31%, respectively, in comparison with the control. In contrast to the no ASD control, both ASD treatments led to greater crown diameters and leaf numbers. At Balm, ‘Florida127’ produced the highest shoot biomass and leaf count, while ‘Florida Brilliance’ maintained the largest crown diameter and high shoot biomass across plant stages. At Citra, ‘Encore’ and ‘Florida Brilliance’ exhibited the highest shoot biomass at final harvest. The relationship between plant growth measurements and fruit yield components will be further examined in the follow-up analysis. Collectively, these results provide a physiological basis for selecting strawberry cultivars for organic systems and understanding genotype by soil treatment interactions toward exploring the linkage between soil health, plant health, and crop productivity.
Speakers
BA

Bikash Adhikari

University of Florida
NA
Co-authors
DM

David Moreira

University of Florida
NA
ER

Erin Rosskopf

USDA-ARS U.S. Horticultural Research Laboratory
NA
IV

Isaac Vincent

University of Florida
NA
JH

Jason Hong

USDA Agricultural Research Service
NA
JD

Johan Desaeger

University of Florda
NA
NX

Nan Xu

University of Florida
NA
XZ

Xin Zhao

University of Florida
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 1
  • Subject Organic
  • Poster # csv
  • Funding Source This work is supported by the Organic Agriculture Research and Extension Initiative program, project award no. 2021-51300-34914, from the U.S. Department of Agriculture’s National Institute of Food and Agriculture
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

1:00pm CDT

ORGH 2 - The Performance of Three Cabbage Varieties Grown Organically with Clover Living Mulches and Reduced Tillage Methods
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Cabbage (Brassica oleraceae var. capitata) is a cool-season cash crop impacted by changing climates and hot summers. Fall-planted cabbage is a common practice hindered by warm-season weed competition during transplanting. This 2023 and 2024 study at the SDSU Southeast Research Farm, Beresford, SD, USA, investigated the impact of established clover living mulch on growth and yield of three fall cabbage varieties with different maturity periods. The varieties selected were Farao (65 DTM), Famosa (81 DTM), and Deadon (105 DTM). One year prior to cabbage planting, three clover cultivars were established: ‘Domino’ white clover (Trifolium repens), ‘Aberlasting’ white x kura clover (T. repens x ambiguum), and ‘Dynamite’ red clover (Trifolium pratense) which were compared to bare-ground control plots. Within each clover whole plot there were four in-row soil management subplots (till, till landscape fabric, no-till, no-till landscape fabric). Combinations of clover, soil management, and cabbage variety were compared for cabbage health, weed suppression, and marketable yield. Harvested cabbage heads were graded in accordance with the USDA Marketable Standard for cabbage cash crop production. Weed biomass decreased as the season progressed and mid-season drought conditions decreased clover growth. The in-row soil treatments demonstrated significant differences for clover and weed biomass accumulation (p < 0.0001). Regardless of variety, cabbage yield and transplant survival decreased within all no-till treatments; this was often due to very small and soft heads. ‘Fararo’ and ‘Famosa’ varieties matured faster than ‘Deadon’, resulting in the cash crop outcompeting weed and clover competition. Clover treatments had the highest impact on the yield of US Number 1 marketable heads (p = 0.02). Cabbage yield within the US Commercial and Unmarketable categories were not impacted by the clover treatments (p = 0.4 and p = 0.06, respectively). The impact of soil management treatments varied depending on the clover variety and cabbage variety combinations. The findings of this study suggest that clover living mulch systems reduce weed competition, minimizing farmers’ workload throughout the season. Within the planting beds, utilization of landscape fabric within no-till treatments is recommended to guard against yield loss. Use of tillage produced marketable crops for all cabbage varieties, yet yield depended on the clover and cabbage variety combination. Organic farmers who grow cabbage within a living mulch system could utilize the living mulch as a benefit for weed suppression in pathways but need supplementary strategies to optimize yield production within the crop row.
Speakers
KH

Kristina Harms

South Dakota State University
Co-authors
JF

Joslyn Fousert

South Dakota State University
NA
KL

Kristine Lang

South Dakota State University
TN

Thandiwe Nleya

South Dakota State University
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 1

1:00pm CDT

ORGH 2 - Using No-till Summer Cover Crops for Transitioning Organic Broccoli Production.
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
As the demand for organic products increases so does the need of organically managed land. In the southeast, where affordable and arable land has become scarcer and more expensive, organic farmers can choose to transition derelict farmland and old pastureland systems to expand current or begin new operations. However, this marginal land is commonly in need of fertility inputs and dominated by perennial weed species. The transition process can prove to be very time and effort consuming. Through the use of no-till drill seeded cover crops, poultry litter applications, and organic fertilizer applications farmers could reduce the thresholds for transitions while potentially reducing weeds, adding instant fertility, and helping build soils for quick turnaround of vegetable cash-crops. Therefore, the objective of this field study was to determine the effects of no-till drill seeded summer cover crops (Sorghum-Sudangrass and Vigna unguiculata) and tarping, with and without the addition of poultry litter, on subsequent fall broccoli production in Georgia. This was a two-year field study on broccoli with the first implementation taking place in the fall of 2023 and the second in the fall of 2024. No-till drilled cover crops and tarps were applied/planted in early May both years with and without the application of poultry litter in a randomized complete split block design. Cover crops were then flail mowed and incorporated in early August both years. Plots were rototilled the first week of September and Brassica oleracea var. italica cv. Belstar was planted with 0.45-m in row spacing in two rows on 0.18-m beds. Broccoli was harvested, counted, and separated into marketable yield or unmarketable yield. Soil cores were taken throughout both the cover crop and cash crop season with measurements of soil nutrients, inorganic nitrogen, and nitrogen mineralization potential being outlined. In 2023 and in 2024 plots that received the combined treatments of silage tarps, poultry litter, and organic fertilizer had the highest marketable yield average of 10013 kg/ha and 8623kg/ha. In 2023 the plots that received the treatments of sudex, no poultry litter, and no fertilizer preformed the poorest in terms of marketable yield, garnishing an average of 2429 kg/ha; while in 2024 the plots that received a silage tarp, no poultry litter, and no fertilizer preformed the poorest with an average marketable yield of 5614 kg/ha.
Speakers
ZH

Zachary Hirsch-Santagata

University of Georgia
Graduate student in the Horticulutre Dept. at the University of Georgia working in organic vegetable production
Co-authors
CS

Charles Smith

University of Georgia
NA
JP

Johnathan Pulliam

University of Georgia
KC

Kate Cassity-Duffey

University of Georgia
NB

Nick Basinger

University of Georgia
NA
TC

Timothy Coolong

University of Georgia
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Organic Horticulture 1

1:00pm CDT

PBIG - Developing Efficient Biotechnological Approaches for Sweetpotato Virus Detection and Removal.
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
As a member of Convolvulaceae family, sweetpotato (Ipomoea batatas L.) is an important crop for food security. As one of the top three vegetable crops grown in Mississippi, one major limitation to sweetpotato production is the cumulative effect of virus infection leading to cultivar decline and yield losses. To produce virus-tested sweetpotato seedlings, we developed meristem-tip culture technology combined with heat treatment to provide farmers with healthy propagating materials that are free of detectable viruses. In this study, totally 30 lines of sweetpotato have been collected in Mississippi and beyond. The plants were first examined with the infection of five of the most prevalent viruses by using nucleic acid-based polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) techniques, which showed high sensitivity and confirmation at the genomic level of viral species and strains. Primers targeting to conserved regions of the know sweetpotato viruses were used for this nucleic acid based detection. The effective protocols for sweetpotato viral detection and viral removal were well developed in this study. The optimized protocols have been used for the purpose of viral detection and eradicating from elite sweetpotato lines in Mississippi. Virus-free planting material has been propagated in Agriculture Research Station of Alcorn State University for performance evaluation.
Speakers
YM

Yan Meng

Alcorn State University
Co-authors
CZ

Chunquan Zhang

Alcorn State University
NA
SB

Sandra Barnes

Alcorn State University
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Plant Biotechnology

1:00pm CDT

PBIG - Development of an inoculation system for powdery mildew of Hydrangea macrophylla
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Bigleaf hydrangea, Hydrangea macrophylla, is a popular ornamental shrub beloved worldwide for its large colorful inflorescences. As of 2019, bigleaf hydrangea topped $155 million in total sales in the United States. However, diseases impact the health, appearance and ultimately the salability of bigleaf hydrangea. One such disease is powdery mildew, caused by the fungus Golovinomyces orontii. Powdery mildew is a biotrophic obligate, which means that it will only grow on a living host, making it particularly hard to investigate. Inoculating plants via tapping infected leaves against clean leaves is the most common inoculation method; however, this method results in an unknown amount of inoculum and inconsistent infection. Quantifiable and consistent methods are needed to accurately and reproducibly study powdery mildew disease of bigleaf hydrangea. The purpose of this experiment was to compare two inoculation methods, an inoculation box and spray inoculation method, for inducing powdery mildew disease in bigleaf hydrangea. For the box inoculation, 6 infected leaves per plant were tapped above a 48-micron mesh and gently dusted through to land on the plant surface. A still air chamber was designed to be set over the box so that powdery mildew does not get disturbed by air currents. For the spray inoculation, 6 infected leaves per plant were combined into a spray by rinsing the leaves with deionized water, adding Tween 20 to aid with even dispersion and applied to the plant using a spray bottle. Powdery mildew was then evaluated on a scale of 0-100% and the area under disease progress curve (AUDPC) was calculated. The final disease severity of hydrangea inoculated via the box and spray method after 4 weeks of observation were 10.0 and 20.0%, respectively. These results will provide valuable information on more consistent inoculation methods using powdery mildew.
Speakers
CJ

Christina Jennings

Tennessee State University
NA
Co-authors
FB

Fulya Baysal-Gurel

Tennessee State University
NA
LA

Lisa Alexander

USDA-ARS U.S. National Arboretum
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

PBIG - Genome editing for enhancing blueberry fruit quality
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Blueberry (Vaccinium spp.) is one of the most economically important woody plant species because its fruit is rich in antioxidants such as anthocyanin, which offer beneficial effects on human health. Thus, the demand for blueberry production and for the development of novel elite cultivars has been continuously increasing. Although new blueberry cultivars have been developed by cross breeding for more than a century, the conventional breeding approaches are time-consuming and labor-intensive due to the associated characteristics that hinder efficient breeding such as long juvenile phase, polyploidy, and heterozygosity. Genetic engineering offers a promising approach to confer a desirable trait to elite cultivars. In particular, the recently developed genome editing technology enables precise modifications of plant genomes. We aim to apply genome editing to improve agronomically important traits in blueberry, specifically anthocyanin and sugar content. Towards the development of high anthocyanin blueberry, we targeted an anthocyanin repressor gene, VcMYBC2. So far, we successfully obtained 3 lines with all-alleles of VcMYBC2 were mutated. An increase in anthocyanin content in fruit is expected in the mybc2 mutants. Regarding sugar content, we targeted invertase inhibitor (INVINH) gene that is involved in sugar metabolism. Invertase promotes sucrose unloading in the fruit by maintaining a gradient of sucrose concentration between source leaves and fruits, while INVINH represses this process. Thus, higher sugar accumulation is expected in invinh mutants. Three transgenic lines harboring CRISPR-Cas9 vectors targeting VcINVINH genes that are highly expressed in fruit tissue, were obtained. The mutated allele frequencies of the mutants ranged from 21-67%. Additionally, we generated transgenic blueberries overexpressing FLOWERING LOCUS T (FT), a mobile florigen signal gene that induces flowering. A previous study demonstrated that blueberry scions grafted to FT-overexpressing lines could show early flowering. We thus assume that the mybc2 and invinh mutants may flower earlier when they are grafted onto the FT overexpressing rootstock, which will accelerate our fruit phenotype evaluations. The FT-overexpressing rootstock may also be utilized to facilitate the production of null-segregant mutants.
Speakers
MO

Masafumi Omori

Kyoto University
Co-authors
HY

Hisayo Yamane

Kyoto University
NA
RT

Ryutaro Tao

Kyoto University
NA
SF

Shuma Fujiyama

Kyoto University
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

PBIG - Genome-wide Mining and Characterization of the SULTR Gene Family in Tomato (S. lycopersicum)
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Sulfur (S) is an essential nutrient present in nearly all proteins as well as numerous secondary metabolites and critical biochemical intermediates. Research across many crop species has demonstrated that post-stress S fertilization can improve plant recovery from various abiotic and biotic stresses. Furthermore, reported findings from these studies suggest that, in the absence of stress, enhanced S fertilization improves the expression of enzymes and metabolites with known stress-defense functions. As part of a new project seeking to test the impact of pre-stress tomato S status on heat stress resilience, we conducted a genome-wide mining and characterization study of the tomato sulfate transporter (SULTR) gene family. Following the typical steps in similar genome mining studies, we identified 14 putative SlSULTR genes through a BLASTp search of known A. thaliana SULTR proteins in the Phytozome database. Then, phylogenetic relationships between the putative SlSULTR genes were characterized using a neighbor-joining tree method after aligning the sequences with the MUSCLE algorithm in MEGA v12. The putative genes were also characterized for their exon-intron structure and intron cycle, which were retrieved from Phytozome and the GSDS server, respectively. Characterization of the putative SlSULTR proteins included transmembrane topology prediction using the SCAMPI2 server and motif analysis using MEME Suite v5.5.7. De novo motif discovery using the MEME algorithm produced 10 amino acid motifs with E-values under 1E-164. These de novo motifs were scanned against Ensembl’s A. thaliana protein database using the MAST algorithm which returned 27 A. thaliana transcripts, each from an AtSULTR gene and with highly significant motif similarities. To characterize the regulation of the putative SlSULTR genes, we searched for SlSULTR-targeted miRNAs in the psRNATarget database as well as identified cis-regulatory motifs in the 1000 bp upstream region of each genomic sequence through the PlantCARE server. Predicted miRNA were from various miRNA families known for their responses to biotic and abiotic stresses and their regulation of nutrient uptake and distribution, plant development, disease resistance, and signal transduction. Lastly, the identified cis-regulatory motifs were largely associated with plant light response but also included motifs associated with general stress and hormone signal response. This work represents the first genomic mining study of tomato SULTR genes. Our phylogenetic and amino acid motif results provide strong evidence that the selected sequences operate as SULTR genes in tomatoes, and the identified miRNA targets and cis-regulatory sequences reflect established research demonstrating the role of S in plant stress response.
Speakers Co-authors
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Plant Biotechnology
  • Poster # csv
  • Funding Source Specialty Crop Multi-State Program

1:00pm CDT

PBIG - Investigation of genotype-dependent in vitro shoot regeneration in highbush blueberry
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Agrobacterium-mediated transformation is widely used in plant genetic engineering. This method involves both Agrobacterium infection and plant regeneration. In this context, establishing an efficient plant regeneration system is a critical prerequisite for genetic engineering in plants. This study aims to identify highbush blueberry (Vaccinium corymbosum L.) cultivars suitable for genetic transformation. Furthermore, we seek to elucidate the molecular and genetic factors that determine genotype-dependent shoot regeneration capacity by utilizing the diverse genetic background in highbush blueberries. Additionally, this study explores cultivar-specific differences in Agrobacterium susceptibility, which remain unexplored in highbush blueberries. Regeneration from leaf explants of 15 highbush blueberry cultivars was investigated on media containing 1.0 mg/L TDZ or 1.0 mg/L TDZ and 0.5 mg/L NAA. There was considerable variation in callus formation and regeneration rates, the number of regenerated shoots, the time required for regeneration, indicating that regeneration in highbush blueberry is highly genotype-dependent. The regeneration rate was high (>75%) in ‘Blue Muffin’, ‘Legacy’, ‘Gulfcoast’ and ‘Georgiagem’. However, ‘Georgiagem’ required three additional weeks for shoot regeneration from the time of meristem formation compared to the other three high regeneration cultivars. All four northern highbush cultivars exhibited low regeneration rates (
Speakers
AT

Arisa Tsuyama

Kyoto university
Co-authors
MO

Masafumi Omori

Kyoto University
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

PBIG - The genome-wide identification and expression analysis of protein phosphatase 2C (PP2C) genes in sweet potato (Ipomoea b
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Protein phosphatases, particularly PP2C families, are vital regulators of cellular activity through the removal of phosphate groups from proteins. Numerous biological processes, such as hormone signaling, reactions to heat and drought stress, and abiotic stress tolerance, are impacted by this dephosphorylation process. This study presents comprehensive genomic analysis, evolutionary assessment and transcript profiling of the PP2C gene family in sweet potato, a crop of major agricultural and nutritional importance. A total of 74 PP2C genes have been identified in sweet potato. Among them, 7 gene pairs were identified as segmental duplication while 5 pairs as tandem duplications. Phylogenetic analysis grouped them into distinct clusters, indicating potential functional divergence. Gene structure analysis provided insights into the arrangement of coding and non-coding regions. Motif and domain analysis highlighted conserved protein sequences aiding functional predictions. Synteny analysis compared genomic regions across species identifying homologous genes and tracing genome evolution. The study of cis-regulatory elements in promoters helped map gene regulations. Gene expression analysis is currently being conducted to determine the transcript levels of PP2C during salinity and drought stress. The results provide a basis for additional validation of the roles of the PP2C gene in sweet potatoes and advance our knowledge of the evolutionary background and functional significance of PP2C in biotic and abiotic stress response.
Speakers
MA

Muhammad Azad

University of Arkansas at Pine Bluff
Co-authors
AG

Ajit Ghosh

Shahjalal University of Science and Technology
NA
MA

Md. Afser Rabbi

Shahjalal University of Science and Technology
NA
SI

Shahidul Islam

University of Arkansas at Pine Bluff
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Plant Biotechnology

1:00pm CDT

PH 1 - Alternative Oxidase Interacts with SNF1 Kinase to Modulate Energy Metabolism During Pear Fruit Ripening
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Alternative Oxidase Interacts with SNF1 Kinase to Modulate Energy Metabolism During Pear Fruit Ripening Adwaita Prasad Parida and Amit Dhingra1 Correspondence: Amit Dhingra, Professor, Department of horticultural sciences, A
Speakers Co-authors
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1
  • Subject Postharvest
  • Funding Source Texas A&M University and Texas A&M AgriLife Startup Funding to the Dhingra Genomics and Biotechnology Lab

1:00pm CDT

PH 1 - Effect of Atmospheric Modification on the Postharvest Shelf-Life of Pawpaw (Asimina triloba)
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Pawpaw (Asimina triloba), a North American tree fruit, belongs to the Annonaceae family and is the only temperate member of this family. Pawpaw is emerging as an alternative high-value niche crop for small farms in Kentucky and surrounding states; however, due to its highly perishable nature, the marketing window for fresh fruit is currently limited. Pawpaw fruit can only be stored for 3-5 days at room temperature or for about 1 month under cold storage at 4 °C. The objective of this study was to examine the efficiency of modified atmosphere with plastic packaging in cold storage to increase the shelf-life of pawpaw fruit and its impact on the quality attributes of fruit. This experiment was conducted with fruit from the pawpaw cultivar Sunflower. Fruits were harvested and stored in three treatments of modified atmosphere in 2.5-gallon plastic container bags, with the treatments of 1) plastic bag with ambient air (Control), 2) plastic bag with an additional ethylene absorber, and 3) plastic bag with modified atmosphere of low oxygen and high carbon dioxide gas mix (12% O2 and 10% CO2 and balance nitrogen). There were three replicate bags per treatment with each bag containing three fruits. Fruits were stored at 6 °C. Data on gas composition in bags and quality parameters of fruit such as firmness, brix, color (L*, a*, b*), and weight were taken on 5, 10, 20, 40, and 80 days of storage. The ethylene absorber and gas mix treatments maintained lower ethylene concentrations inside the bags as compared to the control. The gas mix treatment resulted in reduced fruit weight loss and maintained the lightness of fruit pulp significantly. However, firmness in all fruits significantly declined after 20 days of storage in all treatments. These data suggest that the ethylene absorber and gas mix treatments during cold storage have at least some potential for extending shelf-life of pawpaw, with additional modifications or harvest times.
Speakers
KS

Kshitij Shahi

Kentucky State University
Co-authors
BA

Brent Arnoldussen

University of Kentucky
NA
JL

Jeremy Lowe

Kentucky State University
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
SC

Sheri Crabtree

Kentucky State University
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1
  • Subject Postharvest
  • Funding Source USDA Evans Allen Grant Number KYX--10-17- 63P

1:00pm CDT

PH 1 - Investigations of apple lenticel breakdown and other superficial disorders
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Several apple cultivars are susceptible to lenticel breakdown including but not limited to ‘Honeycrisp’, ‘Red Delicious’, ‘Gala’, and ‘SweeTango’. ‘Honeycrisp’ in particular has received considerable scrutiny by apple growers and packers due to its low percentage of packable fruit. ‘Honeycrisp’ in Michigan are put into storage with little to no disorders and, in 3-5 months, are pulled out of storage achieving less than 50% pack-outs of quality fruit in severe cases. These low pack-outs are a result of internal disorders, a physiological disorder ‘bitter pit’, lenticel breakdown, advanced decay, and some unknown light brown spotting that can be similar or smaller in size than lenticels. Investigations into the mechanisms behind these various ‘spots’ and discriminating between them is an essential first step in understanding the disorders and the discovery of solutions for growers and storage operators. In this study, three preliminary assessments were made to resolve the ambiguity behind lenticel breakdown and associated ‘spots’: 1) apple lenticels were imaged by light and scanning electron microscopy and characterized, 2) ‘Honeycrisp’ and ‘SweeTango’ apples were wrapped in paper and mesh bags on the tree as a field chemical spray exclusion assessment, and 3) a preliminary look at the microbiome of healthy and unhealthy lenticels. The latter effort revealed that ‘Alternaria’ species dominate the fungal community of unhealthy lenticels.
Speakers
AE

Alexander Engelsma

Michigan State University
Co-authors
EL

Emily Lavely

Michigan State University Extension
NA
NR

Nikki Rothwell

Michigan State University Extension
NA
NS

Nobuko Sugimoto

Michigan State University
OH

Ozge Horzum

Michigan State University
NA
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1
  • Subject Postharvest
  • Funding Source Michigan Apple Committee and Project GREEEN

1:00pm CDT

PH 1 - Physiological And Metabolic Responses Of Maturity Levels And Postharvest Treatments In Cold-stored Interspecific ‘Greens
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
The interspecific pear cultivar ‘Greensis’ has become a highly demanded pear cultivar due to its unique flavor of sweetness, crispness and juiciness. This study evaluated the postharvest storage quality of ‘Greensis’ pear at two maturity levels, treated with the 1 µL L-1 1-methylcyclopropene (1-MCP), pre-storage conditioning (PSC), and the combination treatment of 1-MCP and PSC before long term cold storage at 0.5 °C for 6 months. Both the incidence and severity of physiological disorders, such as cortex and core browning, were significantly reduced in PSC treatments, meanwhile, the severity levels of cavity were notably lower in treated fruit compared to untreated (control) fruit. Among the targeted metabolites, soluble carbohydrates and organic acids particularly, sucrose and shikimic acid were lower in mature fruit than immature fruit. Moreover, the contents of glucose, fructose, malic acid and shikimic acids were higher in PSC treated fruit than the other treatments especially in mature fruit. The branched-chain amino acids, including isoleucine and leucine, glutamic acid and phenylalanine, in all treatments were lower in immature fruit, meanwhile, methionine was higher in mature fruit. Additionally, in immature fruit, the contents of asparagine, serine, threonine and proline in the combination treatment of 1-MCP and PSC were higher than the other treatments. In addition, the phenolic contents of all treated fruit were higher in immature than mature fruit especially in chlorogenic acid, arbutin, caffeic, p-coumaric acid, and gallic acid after long term cold storage. Overall, these results suggested that the higher accumulation of phenolic compounds and specific amino acid contents in immature fruit play as a defense mechanism and enhances cold stress tolerance during long-term cold storage. Furthermore, PSC treatments effectively maintain the postharvest fruit quality by reducing the physiological disorders in cold stored ‘Greensis’ pear.
Speakers
TL

Theintthandar Latt

Chung Ang University
PhD candidate at Postharvest Physiology Laboratory, Chung-Ang University.
Co-authors
HP

Hyowon Park

Chung-Ang University
JL

Jinhee Lee

National Institute of Horticultural and Herbal Science
NA
JL

Jinwook Lee

Chung Ang University
NA
JP

Jiwon Park

Chung Ang University
NA
JJ

Joohyuk Jo

Chung Ang University
NA
SB

Si-Eun Byeon

Chung-Ang University
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1

1:00pm CDT

PH 1 - Postharvest Handling and Shelf-Life Evaluation of Three Blackberry Cultivars Under Controlled Storage Conditions
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Blackberry berries are highly perishable fruits, requiring effective postharvest management to maintain quality during storage. This study investigates the shelf-life and postharvest physiology of three blackberry cultivars ‘Ponca’, ‘Freedom’, and ‘Osage’—stored at 0°C for 0 to 7 days and 5°C for 8 to 16 days. Key parameters measured include red drupelet reversion, leakiness, weight loss, acidity (citric, malic, tartaric acid), pH, Brix content, anthocyanin, and antioxidant content. Fresh blackberry fruits of three different cultivars were collected from a commercial garden and stored in 6 oz clamshell boxes under controlled conditions. Physiological parameters such as weight loss, red drupelet reversion, and leakiness were evaluated at 0, 7, 12, and 16 days of storage. For chemical analysis, an additional 6 oz clamshell box for every sample was stored at -40°C at 0, 7, 12, and 16 days to assess acidity, Brix content, anthocyanin, and antioxidant. Results indicate significant varietal differences in postharvest stability. ‘Freedom’ exhibited the highest red drupelet reversion and leakiness, with a steady decline in acidity over time, suggesting rapid deterioration. ‘Osage’ maintained the most stable postharvest quality, showing the lowest red drupelet reversion and highest acidity, which may contribute to improved storage potential. ‘Ponca’ demonstrated moderate stability but exhibited a sharp increase in leakiness and red drupelet reversion at later stages, indicating postharvest instability. Weight loss data revealed that ‘Freedom’ experienced the highest weight reduction, while ‘Ponca’ and ‘Osage’ exhibited greater stability over the storage period. Acidity levels varied significantly among cultivars, with ‘Osage’ retaining the greatest citric, malic, and tartaric acid concentrations, potentially enhancing shelf stability. ‘Freedom’ exhibited the lowest acidity, affecting taste and preservation. pH trends revealed that ‘Freedom’ lost acidity fastest, while ‘Ponca’ maintained stable acidity, making it more suitable for extended storage. These findings highlight the need for cultivar-specific postharvest strategies. While ‘Osage’ appears to have better natural postharvest qualities, ‘Ponca’ and ‘Freedom’ may require optimized storage conditions to minimize quality loss. This study provides valuable insights for improving blackberry storage, reducing postharvest losses, and extending marketability.
Speakers
NS

Nurjahan Sriti

University of Florida
Co-authors
GL

Guodong Liu

University of Florida
JW

Jeffrey Williamson

University of Florida
SS

Steven Sargent

University of Florida/IFAS
Professor and Extension Postharvest Specialist
ZD

Zhanao Deng

University of Florida
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1

1:00pm CDT

PH 1 - Preharvest Ethylene Regulators and their Impact on Postharvest Fruit Quality of ‘Honeycrisp’ Apples Grown in the Mid-Atl
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Reduced fruit quality throughout cold storage is a major stakeholder-identified factor limiting production and marketability of the profitable apple ‘Honeycrisp’ in the Mid-Atlantic. ‘Honeycrisp’ is known to be a chilling sensitive cultivar when cooled immediately after harvest, therefore fruits are submitted to a postharvest conditioning treatment (10ºC for 7 days) prior to cold storage (3ºC). However, conditioning exacerbates the expression of bitter pit, rendering the fruit unmarketable. Moreover, maturity at harvest is a critical factor affecting apple fruit quality as well as susceptibility to chilling stress. Ethylene, the primary hormone related to hastening fruit maturity, can be regulated via the use of preharvest ethylene regulators. We aimed to characterize and compare the effects of different preharvest ethylene regulators on ‘Honeycrisp’ fruit maturity, quality and chilling sensitivity during postharvest. Three preharvest ethylene regulators with different modes of action were applied based on manufacturer’s recommendations, including 1- methylcyclopropene (1-MCP, Harvista), aminoethoxyvinylglycine (AVG, Retain), Ethephon (Ethrel) and a control. Fruits were harvested at two maturity stages: at the anticipated commercial harvest (CH) and one week after CH (CH 1). After harvest, in each case, fruit were submitted to conditioning (10ºC for 7 days) and then stored at 3ºC. Fruit from all 8 treatments were evaluated at harvest, and after 8, 16 and 24 weeks of storage at 3ºC, followed by a shelf-life period (7 days at 20ºC). Our results show that preharvest ethylene regulator treatments impacted fruit maturity and quality of ‘Honeycrisp’ apples during storage. Ethylene production was reduced in 1-MCP and AVG treated fruit, with the latter showing the lowest values throughout storage during both harvest dates. Concurrently, 1-MCP and AVG treated fruits displayed a higher flesh firmness as well as a greener background color (as indicated by higher IAD values) compared to Ethephon- treated and control fruit. Skin blush was negatively affected by 1-MCP and AVG applications, which were maintained throughout postharvest, with AVG-treated fruit exhibiting the significantly lower values. Effects on starch pattern indices and soluble solids contents were inconsistent. Chilling sensitivity, through the presence of soft scald, was not observed, but 1-MCP treated fruit presented the highest bitter pit incidence, followed by AVG-treated fruit, while Ethephon and control fruit displayed the lowest. This study contributes to understand how preharvest ethylene modulation influences ‘Honeycrisp’ apple maturity and quality throughout storage.
Speakers
MS

Md Shipon Miah

University of Maryland - College Park
Co-authors
MF

Macarena Farcuh

University of Maryland
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1
  • Subject Postharvest
  • Funding Source USDA NIFA AFRI GRANT13712074

1:00pm CDT

PH 1 - Red Drupelet Reversion and Consumer Preference of Four Blackberry Cultivars Grown in Georgia
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Blackberry (Rubus spp.) production in Georgia is valued at $19.9 million annually with approximately 1,900 planted acres, mostly concentrated in the southern part of the state. Blackberries have been increasingly planted in Georgia as a dynamic alternative crop that can diversify and support blueberry growers’ income. The harvest season is short, starting in mid-May and ending mid-July. The planted genotypes are mainly from other Southeastern breeding programs often with little to no testing on their suitability for the local growing conditions. Four University of Arkansas-bred cultivars – ‘Caddo,’ ‘Osage,’ ‘Ouachita,’ and ‘Ponca,’ were selected with a goal to identify the most appropriate cultivar for production in Georgia climatic conditions. Blackberries were hand-harvested, field-packed, placed in cold storage for up to 21 days, and evaluated over a two-year harvest period from a commercial field in Holt, GA (31.5957˚N, 83.1507˚W). Red Drupelet Reversion (RDR) is a major postharvest disorder where fully ripe, black drupelets revert to red color after harvest. Assessments for RDR incidence were conducted immediately after harvest and every seven days, along with measurements of berry physicochemical traits. Consumers' taste panels were conducted a day after harvest. A high incidence of RDR was quantified at harvest and after storage for ‘Ouachita,’ followed by ‘Ponca,’ ‘Caddo’ and ‘Osage.’ In addition, the panelists favored ‘Caddo’ blackberries, while ‘Ouachita’ was the least preferred cultivar. Appropriate preharvest and postharvest practices, in combination with timely, gentle harvests should be employed to decrease RDR incidence and maintain fruit quality.
Speakers
VF

Vinicius Fernandes Araujo

University of Georgia
Co-authors
AD

Angelos Deltsidis

University of Georgia
RC

Ramsey Corn

University of Georgia
ZR

Zilfina Rubio

University of Georgia
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1

1:00pm CDT

PH 1 - Sweetpotato Peel Nanoparticle Coatings Reduce Postharvest Deterioration
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Edible nanoparticle coating materials were developed from sweetpotato peel tissue combined with sodium alginate and tested for their efficacy in reducing sweetpotato and potato postharvest deterioration. The bio-based nanoparticle coatings applied as thin films to the surface of sweetpotato roots and potato tubers reduced postharvest weight loss and respiration rate. The sweetpotato peel-based nanoparticle coating formulations included 1% and 2% sodium alginate. To enhance the structural integrity of the coatings, they were cross-linked by immersion in a 1% calcium chloride solution. The crosslinked formulations significantly outperformed non-crosslinked coatings and uncoated samples by minimizing weight loss (to below 2.0%–4.0% versus 3.5%–6.5% in controls) and decreasing respiration rates (to 5-13 mL CO₂/kg·h versus 7-20 mL CO₂/kg·h in controls) over extended storage periods. The enhanced performance is attributed to the formation of a dense, mechanically robust coating matrix. Sweetpotato peel tissue nanoparticles combined with sodium alginate and crosslinked with calcium chloride offer promise as sustainable coating materials for reducing fresh produce postharvest deterioration
Speakers
RA

Ragab Abouzeid

Louisiana State University
Co-authors
DP

David Picha

Louisiana State University
QW

Qinglin Wu

Louisiana State University
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

PH 1 - The Difficulty of Degreening Florida Grapefruit with the “Greasy Green” Disorder
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Fresh grapefruit consumers are trained to expect vivid yellow-orange peel color, depending on the variety, even though peel color has little to do with internal maturity or quality. The greasy spot rind blotch disease has been in Florida for many years, ascribed to a fungal pathogen called Zasmidium citri-griseum (previously named Mycosphaerella citri). Besides causing defoliation, it also mars the peel with tiny black specks that cause the rind to remain green, even after degreening. The spots can coalesce into black patches in extreme cases, sometimes called “pink pitting” or greasy spot rind blotch. Growers had this disease under good management, but within the past five years, fruit with similar symptoms started appearing as early as November, when nighttime temperatures are low, and peel color should improve instead of regreening. The symptoms are like greasy spot rind blotch, but there are few to no symptoms on the leaves of the same trees. Thus, the industry generally refers to the disorder as “greasy green” until a more definitive identification of the causal agent and disease development is determined. In a 2021 grower survey, 89% of respondents experienced the disorder to some extent, with packers reporting losses ranging between $20,000 and $1.7 million. There was no consensus among growers and packers regarding the cause of greasy green or the factors contributing to the problem. While studies are underway to determine if the disorder is indeed related to greasy spot rind blotch, studies were initiated to determine if affected fruit could be degreened effectively, as many packers often ended up abandoning degreening attempts on affected fruit and not using them for the fresh market. Our tests found that even after degreening (5 ppm ethylene at 27oC) red and white grapefruit for up to 5 days, their peel color still was not acceptable (
Speakers
MR

Mark Ritenour

University of Florida - Indian River REC
NA
Co-authors
CH

Cuifeng Hu

University of Florida
NA
EM

Eva Mulandesa

University of Florida
NA
LC

Liliana Cano

University of Florida
NA
MD

Megan Dewdney

University of Florida
NA
MM

Monty Myers

University of Florida
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 1
  • Subject Postharvest
  • Funding Source Citrus Research and Development Foundation, Inc.

1:00pm CDT

PLAST - Evaluation of Mulch Types on Soil Microclimate and Health for Tomato and Lettuce Production in the Central United States
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Maintaining soil health for open field and high tunnel (HT) production of vegetables has become prioritized by growers in recent years. In particular, there have been growing concerns about the sustainability of HT systems. Their hot and dry conditions, compounded by intensive practices, lead to challenges with soil temperature and moisture management, compaction, salinity, and ecosystem health. This report investigates how established and novel mulch types can help growers manage soil temperature, moisture, and health conditions across growing systems and climatic conditions. Tomato and lettuce trials were conducted from 2022 to 2024 in HTs and open-field plots to determine the effect of various mulch treatments (bareground, black plastic film, black woven fabric, paper mulch, white silage tarp and black silage tarp) on soil temperature, moisture, and health. Gravimetric and volumetric soil moisture content were determined weekly and bi-weekly in the lettuce and tomato trials, respectively. Soil temperatures were recorded at 10.2 cm depth throughout the growing seasons for both crops. EC, bulk density, and respiration data were collected at termination of all trials to assess soil health. In tomato, the application of black plastic mulch, fabric mulch, and black silage tarp was associated with higher average soil temperatures (23.7°C-28.9°C), compared to (22.4°C-26.8°C) under white tarp and paper mulch. In the lettuce trials, all mulch types retained soil heat in the early season (0.7°C-2.2°C) compared to bareground. The use of silage tarp maintained up to 21% more soil moisture than the bareground treatment for tomato and all mulches were effective at managing moisture loss. Limited effects on soil health criteria were observed, though higher measurements of bulk density and EC may be associated with increases in soil moisture. Results indicdate that mulch color and material affect soil temperatures and soil moisture in the summer, whereas in fall all mulches may reduce heat loss in the early period of the growing season and soil moisture (5-7%) in the late season. As growers address soil health concerns, an integrated approach will need to be adopted that utilizes multiple practices. This study shows that mulch color and type/material can be selected to alter the microclimate of the soil environment across production systems. More research is needed to determine how these impacts affect long-term soil health. The development of regional growing practices that ensure soil health will be instrumental to support a growing population and using mulches for vegetable production can help achieve this goal.
Speakers
CB

Claire Barnhart

Kansas State University
Co-authors
CR

Cary Rivard

Kansas State University
EP

Eleni Pliakoni

Kansas State University
PT

Peter Tomlinson

Kansas State University
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Plasticulture
  • Subject Plasticulture
  • Poster # csv
  • Funding Source K-State Research and Extension Center for Sustainable Agriculture and Alternative Crops 2023 and Kansas State University GRIP Project

1:00pm CDT

PLAST - Evaluation of Soil Biodegradable Mulches used in Plasticulture System Under Different Climatic Regions of North Carolina
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Evaluation of Soil Biodegradable Mulches used in Plasticulture System Under Different Climatic Regions of North Carolina With Tomato as A Model Crop E. Atley, G. Yang, and T. McGee, Department of Natural Resources and Environmental Design, North Carolina A
Speakers
EA

Elijah Atley

North Carolina A
NA
Co-authors
GY

Guochen Yang

North Carolina A
TM

Trequan McGee

North Carolina A
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Plasticulture
  • Subject Plasticulture
  • Poster # csv
  • Funding Source USDA-NIFA-CBGP-011395

1:00pm CDT

PLAST - Microplastics In Agriculture, A Silent Threat To Ecosystem: Why Biodegradable Pots Are The Future Of Agriculture?
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
The widespread use of plastics in agriculture (plasticulture), including greenhouse films, plastic mulches, plastic pots, seedling trays, pesticide containers and drip irrigation systems, although contributing to short-term productivity, has long-term detrimental consequences for the ecosystem and human health. The accumulation of plastics in soil, chemical leaching from plastics and their degradation into microplastics (particles smaller than 5 mm) is one of the most serious environmental challenges, affecting soil fertility, plant health, and the human food chain. Micro- and nanoplastics and the additives used to enhance the performance and appearance of these plastics can potentially damage tissues and cellular systems by activating various chains of tissue function. Thus, various types of micro- and nanoplastics lead to inflammation, cytotoxicity, genotoxicity and immunotoxicity in cells and tissues. Reproduction is particularly affected by these pollutants, as many of these pollutants can cause endocrine disruption. Efforts to mitigate the negative impacts of plastics include recycling plastic waste, developing biodegradable alternatives, and improving waste management practices. In this regard, Behzist Danesh Narvan Company has used modern technology to produce biodegradable pots (Lignum Vitae) made from completely natural materials such as plant fibers and bio-based adhesive. These flower pots have many advantages as being breathable, 100% biodegradable and ecofriendly, rapid decomposition, having high percentage of organic matter, compatibility with different climates and reducing plastic pollution. These pots can act as a natural fertilizer and enhances plant growth after decomposition.
Speakers
HH

hanieh Hadizadeh

Behzist danesh Narvan Co
I held a Ph.D. in Physiology and Breeding of Ornamental Plants, followed by three years of postdoctoral research at Ferdowsi University of Mashhad, Iran. I also passed one year internship at the University of Georgia, USA. My abstract entitled “Genetic Diversity of a Germplasm Collection... Read More →
Co-authors
NM

Nezhat Mousavi Far

Armaghan surgery center
NA
Wednesday July 30, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Plasticulture
 
Thursday, July 31
 

9:00am CDT

UG 3 - A genome size and ploidy survey of The Morton Arboretum linden collection (Tilia spp. L.)
Thursday July 31, 2025 9:00am - 10:00am CDT
The linden genus (Tilia spp.) is a group of mostly trees native to the northern hemisphere in North America, Europe, and Asia composed of approximately 30 species. As a group of broadly adapted trees, lindens are planted in municipal forests around the world along streets and in parks. Species are known to be somewhat difficult to identify due to the consistently shared morphology of the foliage and flowers as well as notable variation on a single specimen. Additionally, species are capable of hybridization when overlapping in distribution. Ploidy variation within the genus has been reported in the literature as both diploids (2n=2x=82) and tetraploids (2n=4x=164). In order to better understand relationships among species, ploidy should be known. Flow cytomery can be used to determine ploidy and relative genome size. The Morton Arboretum Plant Collections Network accredited Tilia collection includes 58 unique taxa representing 24 species from around the world. The current study uses flow cytometry with the fluorochrome DAPI and Pisum sativum 'Ctirad' as the internal standard to determine ploidy in wild and cultivated lindens. Information from this study can and will be used to inform the development of a genus phylogeny as well as applied in breeding programs.
Speakers
KO

Katherine Opila

The Morton Arboretum
NA
Co-authors
KS

Kim Shearer

The Morton Arboretum
Thursday July 31, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 3
  • Poster # csv
  • Funding Source The Morton Arboretum

9:00am CDT

UG 3 - Effects of Magnesium Fertilization on Chlorophyll Accumulation in Watercress
Thursday July 31, 2025 9:00am - 10:00am CDT
Magnesium (Mg) is a critical macronutrient in plant physiology, playing a central role in photosynthesis, enzyme activation, and energy metabolism. As the core atom in the chlorophyll molecule, magnesium in form of Magnesium Sulphate, directly influences chlorophyll biosynthesis and overall plant health. This study investigated the effects of varying magnesium concentrations from very low (100 mg/L), low (150 mg/L), medium (200 mg/L), high (250 mg/L), on chlorophyll accumulation in watercress. Watercress (Nasturtium officinale) is a fast-growing, nutrient-rich leafy vegetable from the Brassicaceae family. The main objective of the study was to determine the optimal magnesium application level that maximizes the chlorophyll content without causing nutrient imbalances. The experiment was conducted under controlled environmental conditions using a split plot design. Watercress was grown hydroponically using nutrient film techniques throughout key stages of vegetative growth. Chlorophyll content was assessed using SPAD (Soil Plant Analysis Development) meter readings and confirmed through spectrophotometric analysis of chlorophyll extracts. Results revealed that all magnesium treatments led to significant increases in chlorophyll content compared to the untreated control group. The most significancy concentration occurred at 200 mg/L and 250 mg/L concentrations, with 200 mg/L emerging as the most efficient level for promoting chlorophyll accumulation without physiological stress. Enhanced chlorophyll levels correlated with improved leaf coloration; deep green color and overall vigor, suggesting greater photosynthetic efficiency and biomass potential. This study demonstrates that appropriate magnesium fertilization can substantially improve chlorophyll concentration and physiological performance in watercress. These findings offer valuable guidance for growers seeking to optimize yield and quality of leafy greens through targeted nutrient management. Future research should explore supplemental magnesium fertilization effects on biomass accumulation and interactions with other macronutrients.
Speakers
AS

AFFOUE SEPHORA FLAVIE YAO

Kentucky State University
Co-authors
GA

Germaine Akeza

Kentucky State University
Ms. Germaine N. Akeza is a graduate Research Assistant at Kentucky State University, pursuing a master’s in environmental studies. Born and raised in a densely populated Country from a family of subsistence farmers whose farming experience instilled in her the principles of hard... Read More →
RK

Richard Kabanda

Kentucky State University
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Thursday July 31, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 3
  • Poster # csv
  • Funding Source Land Grant

9:00am CDT

UG 3 - Evaluating New Sweetcorn Hybrids for Abiotic Stress Tolerance in the Virgin Islands
Thursday July 31, 2025 9:00am - 10:00am CDT
Many breeding populations of sweetcorn have been developed for resistance to corn earworm, Heliothis zea, using Zapalote Chico (ZC) lines as a main resistance genetic resource. We are evaluating ZC as a source of drought and heat tolerance and to determine if ZC confers such tolerance to Hawaiian Super Sweet (HSS) developed at University of Hawaii. We are comparing the genetic potency of single-eared ZC landrace that is segregating for purple stem and tassel, and green stem and tassel in a hybrid with the the all-green, double-eared HSS. Forward and reciprocal hybrids were developed between HSS for a total of four hybrid sub-groups incorporating the two color sub-groups of ZC and HSS as the parents. The hybrids were developed at the University of the Virgin Islands, agInnovation Research Station. The F1 and the parents were evaluated under three levels of severity of deficit irrigation and exposed to the same level of summer heat. Fresh yield, sweetness and time to harvest were compared among the F1s. Preliminary results show that based on the three metrics we used, the HSS/ZCpurple had a higher tolerance to water deficit than the HSS/ZCwhite, while no significant difference were observed among the reciprocal F1s. The double-eared HSS/_derived hybrids may have the potential to improve sweetcorn production for the Virgin Islands small-scale producers Key words: Abiotic stress, genetics, hybrid, sweetcorn, Zapalote Chico
Speakers
NM

Naseem McIntosh

University of the Virgin Islands
NA
Co-authors
HA

Henry Awika

University of the Virgin Islands
SM

Sydney M Philbert

University of the Virgin Islands
Thursday July 31, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 3
  • Poster # csv
  • Funding Source USDA-AMS-SCBG-VIDA

9:00am CDT

UG 3 - Growing Medicinal Shrubs in the Arid Southwest
Thursday July 31, 2025 9:00am - 10:00am CDT
In the Southwest, increasing water shortages and persistent arid conditions make growing crops difficult. An important question to ask is whether expanding to organic growing systems can support small farms to not only meet water demands but also support the farm’s income. Catering to the environment by selecting medicinal native plants that are drought and heat-tolerant could achieve this goal. Organic farming methods and alternative crop choices play a crucial role in resilience. Our project’s objective is to identify perennial shrubs that hold cultural relevance for small farmers in the Southwest. High-value shrubs with medicinal properties were cultivated using organic farming practices. For the first year in June 2024, we planted chokecherry (Prunus virginiana L.), native plum (Prunus americana M.), and golden currant (Ribes aureum P.), and they were monitored for establishment. In May 2025, survival rates were conducted, only a total of 5 shrubs did not survive out of a total of 36 shrubs. However, 31 perennial shrubs from three species overwintered successfully and have returned for a second year. After year one, we conclude that these native shrubs exhibit high survival rates in the region. For upcoming work, we will conduct replicated trials using water-deficit, well-watered, and normal watering being our control.
Speakers
KR

Kristen Ramos

New Mexico State University
Co-authors
CM

Connie Maxwell

New Mexico State University
NA
IG

Ivette Guzman

New Mexico State University
KL

Kevin Lombard

New Mexico State University
Kevin Lombard is a Professor of Horticulture and Research Director of the New Mexico State University Agricultural Science Center at Farmington, a 250 acre research farm uniquely located on the Navajo Nation. His research encompasses specialty crop evaluations including grapes, fruit... Read More →
RH

Robert Heyduck

New Mexico State University
NA
Thursday July 31, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 3
  • Poster # csv
  • Funding Source USDA NIFA ORG GR0007385
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

9:00am CDT

UG 3 - Temporal Light Quality or Intensity Alternations Elicit Sequence-Dependent Intermediate Lettuce Growth Compared to Fixed
Thursday July 31, 2025 9:00am - 10:00am CDT
In sole-source lighting of indoor-grown plants, blue (B, 400-500 nm) and red (R, 600-700 nm) light are commonly used because of their high photosynthetic photon efficacy. Increasing the B fraction generally decreases plant size and biomass but enhances secondary metabolism. When light is a limiting factor, proportionally increasing B R light promotes electron transport and thus photosynthesis and biomass. Although indoor farming typically uses fixed lighting, dynamic lighting that tailors light quality and intensity for each growth phase has potential to balance desirable traits. We evaluated how temporally alternating the relative and absolute amounts of B and R light influenced growth, morphology, and pigmentation of red-leaf lettuce ‘Rouxai’. We grew plants indoors at 20-22 °C air temperature and 18%-24% relative humidity under a 24-hour photoperiod. After germination under R180 (each subscript denotes photon flux density in µmol∙m–2∙s–1) for 4 days, plants were subject to four fixed light treatments (R180, B90R90, B180, and B180R180) or four temporal light alternations over six 4-day intervals (R180→B180, B180→R180, B90R90→B180R180, and B180R180→B90R90, with each repeating the switch between 4 days of the former treatment and 4 days of the latter treatment). We collected data on seedlings and mature plants 12 and 28 days after seed sow, respectively. For fixed light treatments except B180R180, increasing the B fraction from 0% to 100% decreased shoot biomass by 38%-47% for seedlings and 62%-74% for mature plants, but increased chlorophyll concentration and red coloration. Given equal B and R light, doubling the PPFD increased shoot biomass by 54%-55% for seedlings and 75%-83% for mature plants. Temporal light quality or intensity alternations resulted in similar seedling growth to the latter treatment in the alternating sequence, if fixed. In contrast, repeated temporal light alternations resulted in intermediate mature plant growth between growth under the former and latter treatments in the alternating sequence, if fixed, albeit more resemblant to the latter. Interestingly, given the same total light integrals, the B180→R180 alternation increased shoot biomass by 40%-50% and leaf size compared to fixed B90R90. We conclude continuous or intermittent high B light decreases lettuce growth, and effects of temporal light alternations on lettuce growth are generally in between effects of fixed light treatments, oftentimes resembling effects of the latter treatment in the sequence.
Speakers
TM

Talia McCann

University of Delaware
NA
Co-authors
QM

Qingwu Meng

University of Delaware
Thursday July 31, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 3
  • Poster # csv
  • Funding Source Funding was provided by the National Aeronautics and Space Administration (award # 80NSSC23K1427).

9:00am CDT

UG 3 - Temporally Increasing Light Intensity Produces Similar Lettuce Growth More Efficiently Than Fixed High Light
Thursday July 31, 2025 9:00am - 10:00am CDT
Sole-source light-emitting diode (LED) lighting is indispensable in controlled environments without sunlight, including growth chambers, indoor vertical farms, and spaceflight conditions. Crops like lettuce (Lactuca sativa) are typically grown under fixed light over the entire growing cycle. There is potential to improve light use efficiency through dynamic lighting using dimmable LEDs, but a knowledge gap exists in plant responses to changing light intensities. In an indoor experiment, we characterized how temporal light intensity alternations influenced growth, morphology, and pigmentation of red-leaf lettuce ‘Rouxai’. From day 0 to 28, we germinated and grew lettuce hydroponically at 21-23 °C air temperature and 18%-27% relative humidity under six lighting treatments, including two fixed photosynthetic photon flux densities (PPFDs) of 150 and 350 µmol∙m–2∙s–1 and four temporal PPFD alternations with increasing PPFDs (150→250→250, 150→350→350, 250→250→350, and 250→350→350 µmol∙m–2∙s–1) over three lettuce growth phases [lag phase (days 0-11)→exponential phase (days 11-25)→finish phase (days 25-28)]. All treatments had the same light spectrum (50% warm white 50% red) and 24-hour photoperiod. We collected seedling data and transplanted additional seedlings into Kratky-style hydroponic units on day 11 and collected mature plant data on day 28. Increasing the fixed PPFD from 150 to 350 µmol∙m–2∙s–1 increased seedling shoot fresh and dry mass by 69% and 84%, respectively, leaf number from 4 to 5, leaf width by 22%, chlorophyll concentration index by 15%, and red coloration, while decreasing leaf length by 11%. Similarly, for mature plants, increasing the fixed PPFD from 150 to 350 µmol∙m–2∙s–1 increased shoot fresh and dry mass by 66% and 70%, respectively, leaf number by 23%, leaf width by 11%, and chlorophyll concentration index by 37%, while decreasing light use efficiency (biomass per unit light) by 27%-29%. Compared to the fixed 350 treatment, the 250→250→350 and 250→350→350 alternations resulted in similar biomass, morphology, and pigmentation. However, the 250→250→350 alternation had 23%-31% higher light use efficiency than the fixed 350 treatment. With all treatments taken together, increasing the total light integral from 363 to 847 mol∙m–2 increased shoot fresh and dry mass but decreased light use efficiency. In conclusion, a temporal light intensity alternation produces comparably high biomass in lettuce more efficiently than fixed high light.
Speakers
SD

Sarah Ding

University of Delaware
NA
Co-authors
QM

Qingwu Meng

University of Delaware
Thursday July 31, 2025 9:00am - 10:00am CDT
Empire AB
  Poster, Undergraduate Student 3
  • Poster # csv
  • Funding Source Funding was provided by the National Aeronautics and Space Administration (award # 80NSSC23K1427).

12:15pm CDT

GG - Enhancing Molecular Analysis of Emerald Ash Borer Resistance in Fraxinus Species
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Assessments of genes associated with plant host defense responses can be challenging as the defensive mechanisms that enable the host-mediated defense can be the very compounds that make gene expression assays particularly challenging. RNA extraction from woody plant tissues presents significant challenges due to endogenous phenolics, secondary metabolites, and stem polysaccharides. We have established an improved extraction protocol for Fraxinus species, yielding superior results to commercial kits. Our optimized approach, validated across diverse tissue types from over 10 Fraxinus species, consistently produces high-purity RNA with exceptional concentrations (>3000 ng/μL) and integrity (RIN scores 8.0-10.0). The RNA quality we have achieved allows us to detect and analyze rare transcripts that may play crucial roles in emerald ash borer resistance mechanisms. Our approach enables us to quantify copy numbers of defense genes triggered during insect attacks, shedding new light on the molecular basis of resistance pathways in ash trees. By employing digital droplet PCR and RNA-seq calibrated, we can determine the key defense genes' exact transcript copy numbers, including those encoding protease inhibitors, phenolic compounds, and terpenoid synthases central to anti-herbivory responses. We have validated a stable reference gene suite with reliable quality control and consistent expression benchmarks. These references serve as crucial yardsticks when measuring expression patterns across ash trees with varying levels of EAB vulnerability. By determining actual transcript numbers, we can make more meaningful comparisons between ash species and genotypes, helping us identify the critical expression thresholds needed for effective resistance. This work strengthens our partnership with the Chicago Region Tree Initiative, supporting efforts to build more resilient urban forests and protect endangered ash species. Through our detailed analysis of gene expression profiles across diverse ash populations, we are working to pinpoint the genetic signatures that confer EAB resistance. These findings will enable the development of efficient molecular screening tools (e.g., SNP marker panels, transcriptomic signature profiles, or RNA expression ratio tests) for large-scale population assessment and accelerate efforts for this important genus. (Co-authored by Dr. Nathan Maren, Woody Plant Breeder and Genomics Specialist at The Morton Arboretum).
Speakers
RP

Robert Petursson

The Morton Arboretum
Co-authors
NM

Nathan Maren

The Morton Arboretum
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

GG - Genetic Mapping of Apple Stomatal Traits With Computer Vision
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Stomatal function is a critical determinant of overall plant vigor, health, and yield. Higher stomatal conductance is associated with higher yields, and therefore is a trait of interest for plant improvement. Although stomatal conductance is governed by a complex balance between many factors, stomatal size and density are two traits that set the foundation for a genotype’s response to the external/internal factors. Understanding the genetic architecture of these traits is a key first step in the process of genetic selection; unfortunately, phenotyping stomatal traits on the scale required for mapping studies can be logistically challenging. In this experiment, we microscopically imaged stomata in apple leaves and used two computer vision methods to rapidly phenotype stomatal traits- a convolutional neural network (CNN) and the web-based computer vision platform BioDock. Two apple populations with existing molecular marker information were phenotyped: a biparental mapping population of approximately 400 individuals and the USDA’s Malus germplasm collection. Genetic mapping was carried out using the ‘r/qtl’ and ‘GWASpoly’ packages in R for the mapping population and germplasm collection, respectively. Both computer vision models yielded accuracies >90% for phenotyping stomatal density in the training and validation datasets, demonstrating that these models are effective methods for quickly phenotyping large stomatal image datasets. Preliminary results indicated peaks associated with stomatal density on chromosome 1 and chromosome 7. Furthermore, stomatal density was negatively correlated with stomatal size- resulting in less variation in total stomatal area than either the distributions of distribution or size would indicate. Future work in this project will focus on identifying the genes involved in regulating stomatal density in apples, as well as generalizing the computer vision models to function on multiple plant species.
Speakers
KB

Kenneth Buck

Cornell University
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

GG - Genome-Wide Identification and Functional Characterization of RING-Type E3 Ligase Proteins in Pisum Sativum During Root
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Pea (Pisum sativum) is a valuable legume crop recognized for its rich nutritional profile, offering plant-based protein, fiber, vitamins, and essential minerals. It holds a significant place in the growing plant-based protein industry, which is projected to reach $313.5 million by 2025. However, global pea production is declining due to soilborne diseases, notably root rots caused by Fusarium solani f. sp. pisi (Fsp). In our earlier study, we performed time-course transcriptome analysis on four Fsp-tolerant and four Fsp-susceptible pea genotypes during pathogen infection, identifying several Fsp-responsive genes. Interestingly, the dataset also contained Fusarium-derived genes, many of which encode ubiquitin, ubiquitin-like proteins, and the ubiquitin-40S ribosomal protein S31 fusion protein. EffectorP analysis revealed that these proteins are secretory in nature. We hypothesize that Fusarium secretes these proteins into host cells to manipulate the host’s ubiquitin-proteasome system, leading to the degradation of plant defense proteins. To explore this further, we investigated RING-type E3 ligase proteins in Pisum sativum, which play key roles in protein ubiquitination. A total of 663 genes encoding RING-type E3 ligases were identified, each containing at least one RING domain as predicted by the SMART database. Domain analysis revealed additional conserved motifs within these proteins. An Un-rooted Neighbor-Joining phylogenetic tree grouped the RING proteins based on shared domain architecture. Transcriptomic data indicates that these genes are differentially expressed during Fsp infection. The E3 Ligase genes are upregulated in Fsp-susceptible cultivars and downregulated in Fsp-tolerant cultivars. These genes can be used to generate future knock-out mutants and perform functional studies to enhance pea resistance to Fsp-induced root rot.
Speakers
JM

Josiah Manning

Texas A
M.S. in Horticultural Sciences graduate student at Texas A
Co-authors
AP

Adwaita Parida

A and M Texas, College station
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Genetics and Germplasm
  • Subject Genetics and Germplasm
  • Poster # csv
  • Funding Source Texas A&M University and Texas A&M AgriLife Startup funding to the Dhingra Genomics and Biotechnology Lab.

12:15pm CDT

GG - Optimizing Oxford Nanopore Sequencing Technology for Rapid Onsite Diagnosis of Phytophthora capsici
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Detecting Phytophthora capsici (P. capsici) based solely on visual symptoms is challenging and often leads to misdiagnosis. Farmers frequently harvest seemingly healthy fruits, only for fruit rot to develop after shipping. Furthermore, other pathogens can mimic P. capsici symptoms on cucurbits and peppers, and plants may even suffer simultaneous attacks by multiple pathogens, complicating identification. Without timely and accurate diagnosis, P. capsici can spread rapidly, causing significant crop losses. Current diagnostic methods, including traditional microscopy-based culture techniques and polymerase chain reaction (PCR), are time-intensive and lack sensitivity for early-stage infections. This study introduces an optimized Oxford Nanopore Technology (ONT) genomic approach for rapid and precise detection of P. capsici in plant samples, both in laboratory and field settings. Designed for portability and capable of sequencing reads up to 100 kb, the ONT MinION device—smaller than a smartphone—provides a promising solution for in-field diagnostics. Plant tissue samples, symptomatic and non-symptomatic, were collected from cucurbit and pepper fields through collaborators during late summer and early fall. Total DNA was extracted using a magnetic bead-based kit (Primerdesign, Southampton, UK). Sequencing libraries were prepared using ONT’s 1D-cDNA sequencing kit, loaded onto a MinION 107 v9.5 Flow Cell, and analyzed using the Mk1B MinION device. Raw sequence reads in fast5 format were converted to fastq or fasta, with high-quality reads subjected to BLAST searches against the NCBI database for P. capsici identification. The deployment of ONT enables the generation of actionable genomic data in real-time, enhancing our understanding of P. capsici and its role in Phytophthora blight disease development in cucurbits. This technology represents a breakthrough in the rapid, field-based diagnosis of P. capsici, providing farmers with an efficient tool to mitigate crop losses. Keywords: Oxford Nanopore Sequencing Technology (ONT), Phytophthora capsici, Raw sequence reads, Phytophthora blight.
Speakers
KT

KUMUDINI TALARI

Alcorn State University
Research Associate at Alcorn State University
Co-authors
EA

Emran Ali

Alcorn State University
NA
SW

Sumyya waliullah

Alcorn State University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Genetics and Germplasm
  • Subject Genetics and Germplasm
  • Poster # csv
  • Funding Source NIFA,NM State University, Delta regional, Alcorn Extension Program

12:15pm CDT

GG - Screening Common Bean and Runner Bean Genotypes To Ashy Stem Blight and White Mold
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Ashy stem blight and white mold caused by Macrophomina phaseolina (Tassi) Goidanich and Sclerotinia sclerotiorum L. de Bary, respectively are important fungi pathogens affecting common bean (Phaseolus vulgaris L.) worldwide. Genetic resistance is the most environmental friendly approach to control both diseases. Our objective was to evaluate the response of Phaseolus spp. germplasm to three fungal isolates. Two runner bean accessions (P. coccineus L.), and 23 common bean genotypes including 10 UPR-Mp breeding lines derived from multiple-parent crosses were inoculated with the NY133 S. sclerotiorum isolate and PRI21 and PRI24M M. phaseolina isolates by the cut-stem method in the greenhouse. The disease severity was evaluated at 35 days post-inoculation. Middle American common beans ‘Othello’, TARS-MST1, and ‘Verano’ were susceptible (mean scores > 6.5) to all fungal isolates whereas the runner beans PI 183412 (Sel-1 and Sel-2) and breeding line UPR-Mp-57 were susceptible to NY133. Andean common beans A 195, ‘PC 50’, PRA154, PRA155, and VA 19 were intermediate (scores 4-6) to NY133, PRI21, and PRI24M. In contrast, Middle American beans 92BG-7 and BAT 477 were intermediate to NY133 and PRI21, and susceptible to PRI24M. The runner beans PI 183412-Sel-1 and PI 183412-Sel-2, and common bean breeding lines UPR-Mp-22, UPR-Mp-48, UPR-Mp-54, and UPR-Mp-57 were resistant (scores < 3.5) to PRI21 and intermediate to PRI24M. Conversely, UPR-Mp-34 and UPR-Mp-54 were resistant to NY133. This information should help to select parents with higher levels of resistance that may be used in breeding programs for both diseases.
Speakers
DV

Diego Viteri

University of Puerto Rico at Mayagüez
Co-authors
AL

Angela Linares Ramirez

University of Puerto Rico
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Genetics and Germplasm

12:15pm CDT

GG - Where, when and at what time to phenotype canopy cooling capacity in wheat breeding programs?
Thursday July 31, 2025 12:15pm - 1:00pm CDT
In light of the increasing demand for resilient crops amid global food security concerns, recent advances in omics technologies have accelerated plant breeding efforts. Nonetheless, their effectiveness is often undermined by limited phenotypic resolution, particularly under field conditions. Traditional approaches based on single daily measurements are insufficient to capture the full spectrum of genotypic responses, especially when environmental stress is present. This study explores the potential of thermal imaging using unmanned aerial vehicles (UAVs) to monitor canopy temperature (CT) in wheat, providing a non-invasive proxy for assessing plant water status. A collection of 184 genetically distinct wheat genotypes was examined under both irrigated and rainfed conditions within a Mediterranean agroecosystem. Thermal data were recorded across multiple phenological phases (from anthesis to grain filling) and at various times throughout the day. The analysis revealed that both developmental stage and time of observation substantially influenced CT patterns, thereby impacting the detection of genotype-specific responses to drought. The most pronounced thermal contrasts between irrigation regimes were observed during the milk-dough and dough stages, particularly in the mid-afternoon when vapor pressure deficit (VPD) reached its peak. These insights support the integration of diurnal thermal phenotyping into breeding pipelines as a means to enhance the identification of drought-adaptive traits in cereal crops.
Speakers
GA

Gustavo A. Lobos

Universidad de Talca
Co-authors
AD

Alejandro del Pozo

U. de Talca
NA
CA

Carlos Araya

U. de Talca
NA
FM

Felipe Moraga

U. de Talca
NA
GR

Gonzalo Rios-Vasquez

U. Católica de Valparaiso
NA
HA

Hamza Ali Khan

U. de Talca
NA
HD

Hanns de la Fuente-Mella

U. Católica de Valparaiso
NA
IP

Isadora Perez

U. de Talca
NA
JF

Jesus Flores

Curimapu
NA
JC

Jose Cares

U. de Talca
NA
JP

Josefa Pacheco

U. de Talca
NA
MB

Manuel Bravo

U. Católica de Valparaiso
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Genetics and Germplasm

12:15pm CDT

LOCSY - Central Sierra Shifting Paradigm To Sustainable Organic And Conventional Production Systems
Thursday July 31, 2025 12:15pm - 1:00pm CDT
The regional surveys assess stakeholder needs and shape regional policymaking. The regional survey was conducted in the Central Sierra region (CSR) of California, covering El Dorado, Amador, Calaveras, and Tuolumne counties, with 52 respondents. The respondents were classified as beginner (20 years) farmers selling their produce mainly at the farmers market and local processing units. The survey found that a significant proportion grows organic food (33%) along with conventional food (38%) and prefer in-person extension communication (79%). The survey recorded diverse crop production systems, including wine grapes, apples, olives, walnuts, berries, pears, vegetables, and flowers in the CSR mostly depend upon groundwater trapped in deeper fractured rock formations, followed by surface water from irrigation districts, springs, and seasonal creeks. The survey underscores the shift in interest among farmers for regenerative practices, (a) field-management, for example, irrigation mainly done by plant-based indicators, followed by calendar-based and sensor-based irrigation, showing the potential area for irrigation workshops, (b) input-management (cover crops, no-till, mulching, manure/composting, etc.), as most of them being the resource limited. Our results agree with the Intermountain Region (Utah extension personnel), who found a similar increase in interest among farmers regarding regenerative agricultural practices in a recent survey. This survey emphasizes emerging interest of (a) resource limited farmers in (b) regenerative practices, and (c) in-person extension communication.
Speakers
HS

Hardeep Singh

Farm Advisor, University of California Agriculture and Natural Resources
Co-authors
AS

Arshdeep Singh

California State University Chico
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Local Food Systems

12:15pm CDT

LOCSY - Community and Agriculture Audits in Two Kansas City Communities
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Due to natural disasters, COVID-19, and economic shifts, resilient agricultural food systems have become increasingly important to cities. Research examining the conditions that support resilient agricultural production systems is critical. Grassroots urban agriculture initiatives often arise to help mitigate the effects of disturbances. The ability of local initiatives to absorb and manage shock relies upon access to various forms of capital including human, social, financial, physical, and natural capital. Along with these forms of capital, governance and political infrastructure can greatly influence food system resilience. Food system audit tools have increased in popularity as a way to evaluate a food system’s accessibility to capital as well as examine system gaps and strengths. Many food system audit tools have been created to assess food systems at different system levels for different stakeholder audiences. This project used the audit tool titled the Community and Agriculture Resilience Audit Tool (CARAT), developed by the North American Food Systems Network, to assess how the communities of Kansas City, Missouri, and Kansas City, Kansas are utilizing the assets of their food system to achieve community resilience. During this study, policies, practices, and programs relating to the urban food system were analyzed by using the 101 indicators from CARAT. To analyze these urban food system assets, the researchers conducted interviews and focus groups with key community stakeholders. In these interviews, specific indicators from CARAT were used as questions to gauge the implementation or lack thereof of policies, practices, and programs specifically relating to urban food systems within the two cities. Searches of publicly available websites were also conducted to examine each city’s zoning ordinances and codes. After data collection, the 101 indicators are then scored based on the CARAT scoring guidelines: does not apply (x), no (0), being considered (1), in place (2), and being implemented (3). These scores act as a baseline for the two cities’ food systems and highlight areas of strengths and weakness to community stakeholders to help prioritize actions to enhance food system resiliency. The initial results indicate that there are more community policies, practices, programs in the CARAT themes of Natural Resource Management and Place-based Economics and fewer community policies, practices, programs in the themes of Community Health
Speakers
DK

Dustin Kohn

Kansas State University
Co-authors
EP

Eleni Pliakoni

Kansas State University
RS

Rebekkah Stuteville

Metropolitan Community College- Maple Woods
NA
TJ

Tricia Jenkins

Kansas State University
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Local Food Systems
  • Subject Local Food Systems
  • Poster # csv
  • Funding Source Game-changing Research Initiation Program

12:15pm CDT

LOCSY - Comparison of ecosystem services of high diversity intercropping and monocropping
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Intercropping systems have been used for thousands of years and provide numerous ecological benefits that promote sustainability. This study evaluates the performance of three diverse seed mixes (warm season soil builder, warm season pollinator mix, milpa garden warm season) alongside 3 monocrops: iron and clay cowpeas (Vigna unguiculata), mancan buckwheat (Fagopyrum esculentum), and butternut squash (Cucurbita moschata) in terms of soil health improvement, pollinator support, and productivity. Additionally, the social acceptance of the seed mixes’ appearance and their use in public and private landscaping will be assessed. Soil samples were collected from each plot before and after each growing season to analyze soil health. Plots were observed for five minutes weekly to count the number of flowers and pollinator visits. The harvested produce was weighed and analyzed for nutritional content. Cowpea resulted in a higher average improvement in soil health indicators compared to the three seed mixes. Pollinator activity indicated that the ratio of pollinators to flowers was similar between the monocrop buckwheat and the three seed mix treatments. Butternut squash produced significantly greater weight, calories, and nutrients than the garden milpa. Milpa demonstrated comparable soil health and pollinator support to other seed mixes. These encouraging results suggest that milpa can be an effective tool for sustainable agricultural practices.
Speakers
MB

Molly Boyd

University of Nebraska-Lincoln
NA
Co-authors
CE

Christian Elowsky

University of Nebraska-Lincoln
NA
LL

Louise Lynch-O'Brien

University of Nebraska-Lincoln
NA
RS

Ronald Stephenson

Plant Sciences Hall
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

LOCSY - Connecting Researchers to Small-Scale Farmers: A Case Study with On-Farm Sweetpotato Trials in Western Washington
Thursday July 31, 2025 12:15pm - 1:00pm CDT
There is growing interest in the viability of sweetpotato (Ipomoea batatas) as a direct market crop in the maritime climate of western Washington. In 2023 and 2024, we partnered with researchers at the Washington State University Northwest Washington Research and Extension Center (WSU NWREC) to coordinate on-farm sweetpotato trials across northwest Washington with on-station trials. In 2023, 20 farmers participated in the on-farm trials and in 2024, 33 farmers participated. Many of the participants were small-scale ( 0.0001). This mirrored findings from the on-station trial, indicating plastic mulch or other soil warming strategies are needed to ensure good yield in the region. Qualitative data from farmers based on their experience proved to be highly valuable and informed research directions for the 2025 growing season. For example, slip production and curing can be challenging for many small-scale growers who do not have appropriate facilities, so we initiated trials at the research station on both topics. The on-farm trials provided opportunities to build relationships with farmers through frequent farm visits and surveys responses. Extension- and research-led trials together resulted in a better understanding of crop performance in this geographically diverse region and among growers who each have a different set of management practices and priorities. This information has been added to the growing guide and WSU resources have been tailored to better fit and address the needs of niche-market farmers learning to grow this new specialty crop.
Speakers
LM

Laurel Moulton

WSU Extension Regional Small Farms Program
Co-authors
CM

Carol Miles

Washington State University
LS

Laura Schulz

Washington State University
SG

Samantha Grieger

WSU Extension Regional Small Farms Program
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Local Food Systems

12:15pm CDT

LOCSY - Contributions to Food System Resilience by Urban Agriculture Sites
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Food systems continue to face disruptions, from supply chain breakdowns to climate change and pandemics. Resilience is the ability of a system to respond and adapt to changing circumstances and withstand disruptions. Resilience capacities of urban and peri-urban agriculture sites are important to ensure nutritional security in cities. This project is part of a four-pronged research project covering a transdisciplinary assessment of urban food systems in and around Kansas City; the other three projects include community health; agricultural productivity; and sustainability. This project aims to develop a survey to assess and evaluate the resilience capacities of urban agriculture sites. A literature search was performed and resulted in development five categories of behavior-based resilience capacities for urban agriculture sites: civic engagement; political engagement; social capital; transformational capacity; and land access. A survey based on these categories and using validated questions was developed to collect proxy measurements of resilience capacities for urban agriculture sites. The survey was developed in collaboration with an advisory board of local food system experts. Data was collected using the survey instrument from urban and peri-urban agriculture sites in the following categories: urban production; peri-urban production; intensive production; community garden; training farm; and high school garden. The survey instrument and a follow-up interview were administered to site operators and a truncated survey was given to site employees, volunteers, and visitors. Survey results will illustrate how urban agriculture sites contribute to the resilience of the food system and the community and allow for the comparison of resilience indicators between types of urban agriculture sites. The data will provide a picture of the contributions to food security by type of urban agriculture in the area around Kansas City. The results of the research could be used to help urban agriculture sites become more resilient, as well as influence planning and policy around urban agriculture when seeking to create more resilient communities.
Speakers
KW

Kara Walker

Kansas State University
Co-authors
CR

Cary Rivard

Kansas State University
EP

Eleni Pliakoni

Kansas State University
PB

Priscilla Brenes

Kansas State University
NA
RS

Rebekkah Stuteville

Metropolitan Community College-Maple Woods
NA
TJ

Tricia Jenkins

Kansas State University
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Local Food Systems
  • Subject Local Food Systems
  • Poster # csv
  • Funding Source This work is supported by Kansas State University's "Game-changing Research Initiation Program." Project title: "Development of Resilient Urban Food Systems That Ensure Food Security in the Face of Climate Change."

12:15pm CDT

LOCSY - Fertilizer and Mulch Use for Peppers in an Extensive Green Roof Environment
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Although green roofs offer an alternative growing space to produce fresh edibles for urban consumers, sufficient fertility levels are often lacking in mediums used for extensive green roof applications which can limit plant growth. Therefore, a study was conducted during the 2024 growing season to evaluate fertilizer and mulch use for ‘Candy Cane’ peppers grown in the extensive green roof environment located atop the Southern Illinois University-Carbondale Agriculture Building. Growth characteristics were collected during early-, mid-, and late-season, while fruit were harvested approximately every four weeks (six times total) throughout the growing season. Fertilizer rate and mulch application interactions did not generally influence (P > 0.05) leaf chlorophyll content or plant vigor at any timing. Marketable fruit yields were also not influenced (P > 0.05) by either fertilizer rate or mulch use. These results suggest that the low rate of fertilizer used was sufficient and mulch use is not required to maximize ‘Candy Cane’ pepper growth and yields. Our results indicate that peppers can be grown effectively in extensive green roof environments with proper water and nutrient management.
Speakers
avatar for Gracie Carmon

Gracie Carmon

Graduate Assistant, Southern Illinois University Carbondale
Co-authors
AW

Alan Walters

Southern Illinois University
KL

Kaitlyn Lamaster

Southern Illinois University
SS

Steven Still

Southern Illinois University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

LOCSY - Finding the Sweet Spot: Evaluating 21 Sweetpotato Entries with a Public Taste Test
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Sweetpotato (Ipomoea batatas) is a new crop to western Washington and has been evaluated for yield and wireworm resistance in trials at Washington State University’s Northwestern Washington Research and Extension Center (WSU NWREC) in Mount Vernon, WA since 2019 and in on-farm trials across western Washington since 2023. Trials at WSU NWREC have included over 20 different sweetpotato cultivars and breeding lines, some of which have proven well suited for production in the region due to high yield and/or wireworm resistance. To assess marketability in the region, in February 2025 we evaluated eating quality of 21 sweetpotato cultivars and breeding lines, including nine orange-fleshed, five yellow-fleshed, and six purple-fleshed entries. Participants in the taste test (IRB exempt
Speakers
LS

Laura Schulz

Graduate Research Assistant, Washington State University
I am a PhD student at Washington State University working with Carol Miles on introducing sweetpotato as a new crop in western Washington.
Co-authors
CM

Carol Miles

Washington State University
JW

Jordan White

Washington State University
Thursday July 31, 2025 12:15pm - 1:00pm CDT
  Poster, Local Food Systems
  • Subject Local Food Systems
  • Poster # csv
  • Funding Source e U.S. Department of Agriculture, National Institute of Food and Agriculture, under award number 2022-38640-37490 through the Western Sustainable Agriculture and Education Program under project number SW23951

12:15pm CDT

NUR - An Inexpensive Fertilizer Dispenser Can Reduce Labor Costs and Over-Application, but May Offer Less Dexterity than Manu
Thursday July 31, 2025 12:15pm - 1:00pm CDT
As US nurseries explore ways to decrease labor costs, mechanization and automation technology (AMT) has become increasingly important. AMTs can help nurseries with repetitive, labor-intensive tasks such as fertilization. Our objective was to compare the Fertileeze Pro-35, an inexpensive fertilizer dispenser (
Speakers
KM

Karl McKim

The University of Tennessee
Co-authors
AF

Amy Fulcher

University of Tennessee
GP

Grace Pietsch

University of Tennessee
NA
JJ

Jakob Johnson

University of Tennessee
NA
MV

Margarita Velandia

University of Tennessee
WH

Walker Harrell

University of Tennessee
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Nursery Crops
  • Subject Nursery Crops
  • Poster # csv
  • Funding Source USDA NIFA 2024-51181-4329

12:15pm CDT

NUR - Determining the accuracy and efficiency of commercially available herbicide application equipment used in container nurs
Thursday July 31, 2025 12:15pm - 1:00pm CDT
With costs that can exceed $5,000 per acre, weed management is often one of the largest threats to container nursery profitability due to lack of postemergence herbicide options, need for labor intensive hand weeding, and the demand for weed free ornamentals. Due to high variability in nursery infrastructure, taxa being grown, and grower preferences, no one piece of herbicide application equipment is used at all nurseries in the ornamentals sector. For granular formulations, herbicides are applied with devices ranging from homemade hand-shaker jars, commercially available gravity-flow type hand spreaders, hand-crank hand-held spreaders (i.e. belly grinder spreaders or chest spreaders) and on a larger scale, tractor mounted granular spreaders. Costs for each of these application devices can vary widely and can have a significant impact on the accuracy, distribution pattern, and efficiency of the application, all which effect weed control and labor costs. For example, hand-shakers might be used to treat pots individually to reduce herbicide waste, that is, the herbicide landing outside of the pot during an application. This is a slower process but results in less non-target herbicide loss compared with a belly-grinder in which over 50% of the herbicide applied may not reach the target depending upon pot spacing. The L.E.A.P. (Labor, Efficiency, Automation, Production) team is currently working to evaluate, implement, and develop automation to increase the sustainability of the nursery industry. As part of this work, commonly used and commercially available herbicide application equipment is being evaluated for accuracy, distribution patterns, efficiency, efficacy and crop safety. Prior to initiation of this work, studies were conducted to determine most accurate method of measuring application equipment performance, specifically for granular applicators. Commercially available herbicide pans, plastic and glass plastic beakers, and small plastic funnels (5 cm diameter) with end caps were all tested in multiple different arrangements with 1 to 12 collection devices placed within blocks of nursery containers containing recently potted ornamentals in 3.8 L nursery pots. The use of the plastic funnels placed sunken in the potting substrate was determined to be the most accurate method, in which 82% to 120% of the target dose was captured across all four plant canopies and was the most practical method to implement as it could be used accurately regardless of canopy structure or pot spacing and is ideal for on-farm evaluations. Project was supported by LEAP Nursery Crops Toward Sustainability Award No. 2024-51181-43291.
Speakers
CM

Chris Marble

University of Florida/IFAS
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Nursery Crops
  • Subject Nursery Crops
  • Poster # csv
  • Funding Source USDA-NIFA-SCRI Award No. 2024-05427.
  • Funding Option SCRI funded all or part of the research associated with this abstract

12:15pm CDT

NUR - Determining the Effects of Increased Controlled-Release Fertilizer on Growth and Physiological Responses of Three Native
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Native edible berry plants create additional opportunities for native plant growers and nurseries due to their dense nutrient content. However, their growth and physiology under nursery conditions have not been widely studied. Controlled-release fertilizers (CRFs) are commonly used in nursery production, yet their optimal application rates for native edible berry plants remain understudied. While overfertilization can lead to excessive nitrogen (N) leaching, underfertilization can limit plant growth. The objectives of this research were to determine the effects, in a greenhouse, of increased CRF rates ranging from 0 to 0.96 g·L⁻¹ N on the development and physiology of three native edible berry species: Elaeagnus commutata (silverberry), Rhus trilobata (skunkbrush), and Shepherdia argentea (silver buffaloberry). For each species, thirty plants were randomly allocated to receive 15N–3.9P–10K CRF treatments at five different nitrogen concentrations: 0, 0.12, 0.24, 0.48, and 0.96 g·L⁻¹ N. These application rates corresponded to 0%, 25%, 50%, 100%, and 200% of the manufacturer's recommended dose. Throughout the 50-day experimental period, all plants received manual irrigation with tap water. Across all three species, higher CRF application rates led to increased leachate electrical conductivity (EC) and nitrate-nitrogen (NO₃-N) concentrations, as well as enhanced relative plant growth index, total leaf area, leaf dry weight, photosynthesis rate, and stomatal conductance. Lower CRF rates resulted in decreased chlorophyll content, photosystem II efficiency, and leaf nitrogen content in E. commutata and R. trilobata, though root-to-shoot ratios were higher at these lower rates. Physiological parameters such as photosynthesis and stomatal conductance showed no significant increases beyond the 0.12 g·L⁻¹ N CRF rate, while growth parameters remained statistically similar at CRF rates above 0.24 g·L⁻¹ N. This research demonstrates that CRF application rates below the manufacturer's recommendation were sufficient to maintain growth and physiology of the three native edible berry species. Under our experimental conditions, the optimal application rate was determined to be between 0.12 and 0.24 g·L⁻¹ N, which effectively sustained plant growth and physiological responses while minimizing NO₃-N concentrations in leachate. This reduction in fertilizer application could decrease production costs for native plant growers and nurseries, providing both environmental and economic benefits while expanding opportunities in the growing market for nutrient-dense native edible plants.
Speakers
avatar for Ji-Jhong Chen

Ji-Jhong Chen

University of Wyoming
NA
Co-authors
DC

David Claypool

University of Wyoming
JF

Jill Fabricius Keith

University of Wyoming
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Nursery Crops
  • Subject Nursery Crops
  • Poster # csv
  • Funding Source United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) Hatch Regular Research Funds (Project accession no. 7003535), University of Wyoming College of Agriculture, Life Sciences, & Natural Resources, Office of Research and Economic Development Designated, Department of Plant Sciences. United States Department of Agriculture National Institute of Food and Agriculture (NIFA) Plant Sciences Hatch Funds (WYO-631-22)

12:15pm CDT

NUR - Effects of Pot Type and Fertility on Initial Growth of Galactia microphylla for Tuber Transplant Production
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Advancing restoration and conservation of coastal dune species improves dune ecosystem function by maintaining biodiversity, mitigating habitat degradation, and reducing erosion through stabilization of deteriorating dunes. Galactia microphylla (Littleleaf Milkpea) is a leguminous coastal dune species found in the Florida panhandle which serves as the sole food source for beach mice (Peromyscus polionotus) for more than one month in fall. We conducted a container production experiment to determine the effects of 3 gallon pot type (tall and short) and fertility regimes (Osmocote 15-9-12 plus minors; 3 month formulation) with 1, 2, or 3, 15 g applications applied at a maximum of 15 g per month over 3 months. The experiment was a randomized complete block design with a split-split plot arrangement of treatments restricting randomization with pot type randomly allocated within each block and fertilizer application randomized within each pot type. There were nine single pot subsamples per treatment for a total of 324 pots. Each pot was filled with the same volume of 100% pine bark mulch with fines and planted with 8 (tall pots) or 12 (short pots) heat scarified seed on week 20 (April 16, 2024). Fertilization began week 24 (June 10, 2024) and emergence (%) recorded June 13, 2024. First flower and first fruit (green capsules) were recorded by production week, and mature fruit harvested when brown to evaluate seed production potential. Emergence was 69.3% with no difference between pot types. First flower (Weeks 34–35) and first fruit (Week 36) did not differ among pot types or fertility regimes. Fruit (2 fruits/pot) and seed number (7.6 seed/pot), seed per fruit (1.5) or seeds per plant (1.2) did not differ between the pot types or among the three fertilization regimes. Results suggest bulk container production of Galactia microphylla has potential in both pot types and that flowering, fruiting and seed production are not improved with fertilization regimes providing greater than the standard (30 gram) Osmocote application. Additionally, there was no evidence of a difference in flowering, fruiting, or seed production when fertilization exceeded the standard level of application. Overall, this experiment provides support for bulk container production of Galactia microphylla for tuber production and suggests the lowest quantity of fertilizer tested was sufficient to achieve fruit and seed production.
Speakers
MT

Mack Thetford

University of Florida
Co-authors
DM

Debbie Miller

University of Florida
NA
TS

Tabitha Slattery

University of Florida
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Nursery Crops
  • Subject Nursery Crops
  • Poster # csv
  • Funding Source Funding provided in part from US Fish and Wildlife Service project F23AC01606 and F21AL02217, USDA National Institute of Food and Agriculture McIntire Stennis projects FLA-WFC-005653 and FLA-WFC-006306

12:15pm CDT

NUR - Establishing In Vitro Propagation Protocols for the Conservation of Parrotia spp.
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Parrotia subeaqualis, a recently introduced plant species in the US, exhibits limited genetic diversity due to the limited collection area. Mirroring the declining populations in their native China, limited genetic sampling can impair cultivar development and threaten cultivars' long-term survival. Conservation efforts and providing the initial plant material for breeding programs rely on efficient and dependable propagation methods. This research focuses on developing optimized in vitro establishment and regeneration protocols for various genotypes of Parrotia spp. We conducted experiments to identify the most effective treatment options and media compositions for in vitro initiation and plant regeneration. These experiments explored using different tissue types collected throughout the growing season, basal media, plant growth regulators (PGRs), and other additives. Initial trials with P. subaequalis were unsuccessful due to heavy contamination. Systematically testing different combinations, we determined the specific stimuli required to induce regeneration-competent tissue for diverse Parrotia lineages. Subsequent experiments on P. subaequalis revealed that the most effective media for P. subaequalis was Murashige
Speakers
NM

Nathan Maren

The Morton Arboretum
Co-authors
KG

Ketniel Guzman

The Morton Arboretum
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Nursery Crops
  • Poster # csv

12:15pm CDT

NUR - Labor, Efficiency, Automation, Production: LEAP Nursery Crops Toward Sustainability
Thursday July 31, 2025 12:15pm - 1:00pm CDT
LEAP is an acronym for investigating the complex nursery specialty crops production system to identify both the individual and interactive effects of Labor, Efficiency, Automation, and Production on current and future nursery industry sustainability. This system is currently experiencing a crisis of labor availability. Our goal is to develop new automation and through better understanding of diffusion of innovation stimulate adoption of existing technologies to study their effects on the remaining system components, including consumer preference at the retail level and the effect of labor retention and re-allocation as a buffer against ongoing labor scarcity. This dynamic approach is necessary, given the complex nature of the nursery specialty crops system that encompasses culturally diverse owner-producer-employee relationships in terms of labor, with high needs for many physically demanding tasks, biological systems, machines, technology, energy, and natural inputs, i.e., water and other resource, all overlaid by preferences and demands of widely different consumer groups. To prevent that labor availability crisis from becoming catastrophic, LEAP and its AB recruited experts at the federal and state level in both plant and social sciences, economics, mechanical engineering, robotics, and artificial intelligence to create five powerful, culturally and disciplinarily diverse teams with expertise in Production and Robotics Engineering (PE), Socioeconomics (SE), Behavioral Adoption (BA), Consumer Preference (CP), and Extension and Science Communication (EX). These teams will work collaboratively with the advisory board, stakeholders, producers, county and regional Extension staff, and allied industries to accelerate the diffusion and impact of automation adoption through nurseries and their workers to illustrate technology’s inherent effect on output, labor efficiency and productivity, revenue, rural economics, and nursery sustainability.
Speakers
CM

Chris Marble

University of Florida/IFAS
Co-authors
AL

Alicia L Rihn

University of Tennessee
AF

Amy Fulcher

University of Tennessee
AL

Anthony LeBude

NC State University
GK

George Kantor

Carengie Mellon University
NA
HG

Hao Gan

University of Tennessee
NA
HZ

Heping Zhu

USDA-ARS
NA
HL

Hui Liu

University of Tennessee
NA
JA

James Altland

USDA-ARS
NA
KA

Karla Addesso

Tennessee State University
NA
LW

Laura Warner

University of Florida
NA
LC

Le Chen

University of Tennessee
NA
LN

Lloyd Nackley

oregon state university
Lloyd Nackley is a plant ecologist who applies a systems approach to improve nursery and greenhouse management. Nackley's research program at Oregon State University focuses on addressing four challenges facing nursery and greenhouse production in Oregon: irrigation application, pest... Read More →
MP

Marco Palma

Texas A
NA
MV

Margarita Velandia

University of Tennessee
MS

Md Sultan Mahmud

University of Georgia
NA
NB

Natalie Bumgarner

University of Tennessee
SS

Susan Schexnayder

University of Tennessee
NA
TR

Taylor Ruth

University of Tennessee
NA
VT

Vashti Tatman

University of Florida
NA
WH

William Hered

Carnegie Mellon University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Nursery Crops
  • Subject Nursery Crops
  • Poster # csv
  • Funding Source U.S. Department of Agriculture, National Institute of Food and Agriculture, SPECIALTY CROPS RESEARCH INITIATIVE. Labor, Efficiency, Automation, and Production: LEAP Nursery Crops Toward Sustainability CAP 2024-51181-43291
  • Funding Option SCRI funded all or part of the research associated with this abstract

12:15pm CDT

NUR - Screening of a Biochemical Pesticide for the Management of Rose Rosette Disease
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Rose rosette disease (RRD) is a major threat to rose production in nurseries. The causal agent of the disease is a negative strand ssRNA virus called Rose rosette virus (RRV) belonging to the genus Emaravirus. RRD is transmitted by the eriophyid mite Phyllocoptes fructiphilus and grafting. Symptoms of the disease include excessive lateral shoot growth, an abundance of thorns, witches' broom, leaf proliferation, deformed leaves and flowers, mosaic patterns, red pigmentation, and eventually plant death. The aim of this study was to evaluate the effectiveness of novel fungicide treatments, NinjaTM, at various application rates, intervals, and methods in managing RRD. Treatments were applied preventively as a sprench (7-day interval) or drench (14-day interval). Pink Knock Out® rose plants were inoculated with RRD on the same day as fungicide treatments. For inoculation, rose plants were exposed to two mite-infested rose terminals (approximately 4 inches long, with 4-5 leaves), collected from RRD symptomatic shrubs in Rutherford Co., TN. Each plant received about 20 mites. The experiment was arranged in a completely randomized design with ten single-plant replications and conducted twice in a quarantined greenhouse at the Otis L. Floyd Nursery Research Center in McMinnville, TN. At the end of the trials, data on the total number of shoots, number of shoots exhibiting RRD symptoms, open flowers, and phytotoxicity were collected. Two sepals from each plant were randomly selected for mite quantification under a dissecting microscope. The proportion of shoots with RRD was calculated by using the number of affected shoots to the total number of shoots. Rosette severity was rated using a scale of 0-3, where 0= no rosettes, 1= one rosette, 2= two rosettes and 3= three or more rosettes. RRD severity was rated on a scale of 0-3, where 0= no symptoms, 1= one shoot with symptoms, 2= two shoots with symptoms, and 3= three or more shoots with symptoms. Results indicated no significant difference in RRD severity between treated and non-treated control plants. However, plants treated with 11 oz NinjaTM exhibited a significantly lower rosette rating compared to the control plants. Furthermore, both the 8 oz and 11 oz NinjaTM treatments significantly reduced the proportion of shoots with RRD. No significant differences were found in mite count per sepal or open flowers among any of the treated and non-treated control plants. These findings suggest that biochemical pesticide treatment may offer a promising strategy to reduce RRD infection in roses.
Speakers
CJ

Christina Jennings

Tennessee State University
Co-authors
CO

cansu oksel

Tennessee State University
FB

Fulya Baysal-Gurel

Tennessee State University
NA
KD

Kumuditha D Hikkaduwa Epa Liyanage

Tennessee State University
NA
PL

Prabha Liyanapathiranage

Tennessee Department of Agriculture
NA
TS

Terri Simmons

Tennessee State University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Nursery Crops

12:15pm CDT

POM 2 - Apple Orchards of Sonoma County - UC Cooperative Extension
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Sonoma County, California is home to many small- to mid-scale apple orchards with diverse cultivars, management approaches and sales outlets. This poster will outline recent UC Cooperative Extension apple programming that responds to high-priority needs identified by apple growers, orchardists, and processors in this region. The new UCCE Specialty Crops Advisor based in Sonoma, Marin, and Napa Counties conducted a Needs Assessment that identified key issues and challenges for sustainable apple orchard system management. This poster will summarize key findings from the Needs Assessment. Stakeholders asked for educational programming on key topics to improve apple orchard health and yield. In response, outreach and Extension education activities focused on science-based sustainable apple orchard management strategies to accelerate practice implementation. This poster will outline these Extension efforts that responded to the Needs Assessment findings and will present evaluation survey results. For instance, stakeholders specifically asked for apple orchard management webinars, so the CE Advisor worked with local and UC partners to deliver a 5-part educational apple webinar series covering key aspects of sustainable apple orchard management. Findings showed that webinars increased attendees’ knowledge (100% of 35 respondents) and 97% reported they will use knowledge gained to manage apple orchards more sustainably. The CE Advisor worked closely with commercial apple growers to address tree health challenges in the orchard on a case-by-case basis. The most common pest issues in the region include codling moth, a suite of diseases, and rodents. Many apple orchards are managed as low-input systems, so tree water stress and nutrient deficiencies are common. The CE Advisor used science-based information and strategies to help growers address these issues. With grower permission, local apple orchard growers’ “Success Stories” are included in Extension educational presentations and materials to highlight examples of sustainable apple orchard management. This poster will include several of these “Success Stories” highlights. This poster will present findings outlining apple growers’ needs for Extension education and applied research in Sonoma County, California. It will outline UCCE activities implemented to increase growers’ practical knowledge and skills to guide sustainable decision making, increase orchard health and yield, and promote grower-to-grower knowledge sharing. Findings will help guide future work to enhance apple orchard sustainability and apple supply for the local food system.
Speakers
EA

Ellie Andrews

Specialty Crops Advisor for Sonoma, Marin, and Napa Counties, UC ANR
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 1
  • Subject Pomology
  • Poster # csv
  • Funding Source UC ANR

12:15pm CDT

POM 2 - Evaluation of 4-Vita Biostimulant and Promalin in Mitigating Green Spot in ‘WA 38’ Apple
Thursday July 31, 2025 12:15pm - 1:00pm CDT
With more than 20 million of trees planted in Washington State, ‘WA 38’ apple, commercialized as ‘Cosmic Crisp®’, is channeling the interest of the apple industry. Since the variety hit the market in 2019, consumers reported positive eating experiences and appreciation for its crispiness and juiciness. In suitable growing conditions, ‘WA 38’ can achieve high red coloration and satisfactory pack-out. One of the causes of loss is the ‘green spot’ (GS) disorder that impacts mainly the stem-hemisphere of the apple displaying skin discolored areas that might degenerate affecting the cortex. The onset of the GS occurs at the end of July, and the disorder incidence at harvest fluctuates year to year. Several mitigations approaches were investigated, but only bagging fruit at early stage of development provided positive results. Because the bagging practice is not economically sustainable on commercial scale, the application of a new foliar biostimulant (Plants for Plants 4-Vita) and Promalin (GA4 7 BA) were tested against untreated control. 4-Vita aims to protect the trees by abiotic stressors like drought and heat, stimulating water use efficiency and productivity. On the other hand, Promalin is known to improve fruit shape and size and reduce russeting by increasing epidermis cell elasticity. Our hypothesis focused on the potential positive effects of a biostimulant and a PGR in mitigating the GS in ‘WA 38’. The products were tested in season 2023 on a Granny Smith/M9-T337 orchard top-grafted in 2015 with ‘WA/38’ scions and trained to spindle, by applying them separately on distinct rows, bi-monthly, starting at 4 weeks AFB for 4 times. Productive parameters, pack out and GS incidence GS were assessed for each treatment. Results showed that Plants for Plants 4-Vita and Promalin did not improve yield and did not significantly mitigate the incidence of GS in comparison to untreated control.
Speakers
SM

Stefano Musacchi

Washington State University
Co-authors
SS

Sara Serra

Washington State University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 1
  • Subject Pomology
  • Poster # csv
  • Funding Source WSU Endowment

12:15pm CDT

POM 2 - Field Evaluation of Six Grafted Cherry (Prunus avium) cultivars under Northwest New Mexico Growing Conditions
Thursday July 31, 2025 12:15pm - 1:00pm CDT
RELEVANCE Sweet cherries (Prunus avium) have some historical production in the Four Corners area with renewed interest for fresh eating, baking, juicing and distilling. RESPONSE Six grafted cultivars were planted March 2014 as bare-root, 3-year old tree seedlings on a high mesa location at the NMSU Agricultural Science Center at Farmington at 5,640 feet above sea level (36°4’ N by 108° W). Cultivars ‘Bing’, ‘Skeena’, ‘Rainier’, ‘Benton’, ‘Santina’ and ‘Coral’ were grafted onto either Krymsk 5 or Krymsk 6 rootstock. Yield data was collected for 2019 and 2021 growing seasons. Trees were pruned during winter dormancy beginning in 2020 to control height. Lower yields in 2021 compared to 2019 are attributed to extensive pruning and to bird damage that was noticeably more impactful in 2021 compared to 2019. In all other years since planting, killing spring frosts reduced or eliminated flower production resulting in little to no fruit yield. When harvested, fruit sugar content (measured in °brix; the higher the °brix, the sweeter) ranged from 15.3° to 22.1° brix in 2019 and 13.3° to 24.6° in 2021. Sugar content is acceptable for any number of post-harvest fresh market to fermentation applications. IMPACT Cherry trees are among the first fruit trees to break dormancy on a high mesa site in Northwest, NM and have been prone to damaging spring frosts resulting in flower death and reduced fruit yields. When cherry have yielded, post-harvest metrics have favorable sugar content. Growers can expect to have non-bearing years related to spring frost risk and are advised to have cherry plantings as part of a diversified cropping system to lessen risk during non-fruitful years. PUBLIC VALUE STATEMENT The work is helping to support northwest New Mexico fruit growers.
Speakers
KL

Kevin Lombard

New Mexico State University
Kevin Lombard is a Professor of Horticulture and Research Director of the New Mexico State University Agricultural Science Center at Farmington, a 250 acre research farm uniquely located on the Navajo Nation. His research encompasses specialty crop evaluations including grapes, fruit... Read More →
Co-authors
BM

Bernd Maier

Amaro Winery and Taproom
NA
CV

Ciro Velasco-Cruz

New Mexico State University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 1
  • Subject Pomology
  • Poster # csv
  • Funding Source New Mexico Attorney General’s Office (GR0007668) through the Northwest New Mexico Agriculture Restoration Project

12:15pm CDT

POM 2 - High tunnel stone fruit production in northern New Mexico
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Late spring frost is a major challenge to stone and pome fruit production in northern New Mexico. In this study, three cultivars of peach (Prunus persica)—Challenger, China Pearl, and Contender on Nemaguard rootstocks were planted at 4x10ft planting density with three replications in a 30x72ft high tunnel in April 2017, but severe leaf chlorosis appeared later in the season. We kept the better west row and removed the central and east row. In 2018, tissue-cultured GF677 and Rootpac® R were planted in May and budded in August 2018. Trees were trained to open vase system. Thermostat-controlled propane heaters and fans were employed for frost protection. In 2021, we began securing the sidewalls and the doors of the high tunnel and setting up heaters. In 2022 and 2023, the high tunnel system plus heating system was sufficient to protect blooms and fruitlets from late frosts, and yields averaged 15.8 kg/tree in 2022 and 12.3 kg/tree in 2023, respectively. There was no significant difference among cultivars in either year. However, there were significant differences between rootstocks in 2022, with trees on Nemaguard averaging 24.3 kg/tree across cultivars, while trees on GF677 and Rootpac® R averaging 11.2 and 11.8 kg/tree, respectively. The nearly two years older in age for trees on Nemaguard could contribute to this difference in 2022. There was also a reasonable crop in 2024. By the end of 2024, Trees on Nemaguard with 1-2ft new growth were smaller than plants on GF677 and Rootpac® R, with 1.5-4 ft new growth. There were limited replications in this high tunnel study but GF677 and Rootpac® R were more suitable than Nemaguard rootstock for the high pH soil conditions in New Mexico. Cherry had limited fruit set during this study. In 2022 and 2023, we observed deformed flowers without petals and some flowers with blackened pistils. Only handfuls of cherries were harvested in both 2022 and 2023. The high temperatures inside the high tunnel in summer could affect the floral bud initiation/ development. More studies are needed to clarify the deformed flowers. Growers should be cautious with sweet cherry production in high tunnels in northern New Mexico.
Speakers
SY

Shengrui Yao

New Mexico State University
Co-authors
RH

Robert Heyduck

New Mexico State University
NA
SG

Steven Guldan

New Mexico State University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 1

12:15pm CDT

POM 2 - Irrigation And Foliar Fertilization Effects On Yield And Fruit Quality Of Peaches.
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Previous studies in the southeastern United States have reported benefits of irrigated vs non irrigated orchards, including no major effects of soil-applied fertilizers on fruit yield and quality. However, the benefits of irrigation systems and foliar fertilization effects on yield, and fruit nutrient profiles and quality are limited. This study was conducted in a nine-year-old peach orchard using a split-plot randomized complete block design with two irrigation systems, drip vs micro-sprinkler as whole-plots, and foliar treatments as split plots. Preharvest foliar applications included water (control), nanocellulose crystals (CNC 3%), Calcium (Ca 6%), Potassium Silicate (K2SiO3), plus the combination of CNC with Ca and K2SiO3. Trees received no soil-applied fertilization, and irrigation was managed using the Peach Smart Irrigation App recommendations (https://smartirrigationapps.org/peach-app/). Even though no significant differences were observed in irrigation systems or foliar treatments for fruit weight or perimeter, all foliar treatments resulted in higher total yield compared to the control under drip irrigation. Under micro-sprinkler irrigation, only K2SiO3 and CNC increased total yield relative to the control. Regarding texture, control fruit showed decreased firmness after two weeks of storage at 2 °C and 90% relative humidity. There were main effects of irrigation in total soluble solids (TSS), where micro-sprinkler (10.27%) had higher TSS than drip irrigation (9.67%). Overall, these findings suggest that drip irrigation and foliar applications, particularly with CNC and K2SiO3, may enhance yield in peaches.
Speakers
SC

Selvin Carrillo Ramos

University of Georgia
Co-authors
DC

Dario Chavez

University of Georgia
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 1

12:15pm CDT

POM 2 - Visualization of Regional Fruit Growth Rate in Persimmons Using 3D Phenotyping
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Understanding fruit growth and developmental patterns is crucial, as they directly influence fruit size, morphology, and ultimately, yield. While conventional models describe fruit growth and development based on parameters for the whole fruit, how tissue growth in different fruit regions is coordinated to drive overall fruit growth and development remains unclear. Here, we investigated the relationship between the spatial variation of growth rates and fruit morphology in persimmon (Diospyros kaki), a fruit tree species with highly diverse fruit morphology. Starting two weeks after blooming, fruits from four cultivars were marked with dotted lines routinely. Those fruits were sampled and used for the reconstruction of 3D fruit models. The relative positions and distances between points marked were used as parameters for the local growth. All cultivars exhibited more active growth near the calyx than near the apex, particularly during the early stages of fruit development. However, the extent of these spatial differences varied among cultivars. In flattened fruits, growth was highly concentrated near the calyx, with remaining minimal in other portions. In contrast, elongated fruits exhibited a gradual growth gradient from the calyx toward the apex, with high growth even near the apex. These findings provide a foundation for fruit shape regulation and elucidation of physiological disorders in persimmon.
Speakers
AK

Akane KUSUMI

Graduate school of Agriculture, Kyoto University
Co-authors
RT

Ryutaro Tao

Kyoto University
NA
SN

Soichiro Nishiyama

Kyoto University
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Pomology 1
  • Subject Pomology
  • Poster # csv
  • Funding Source Japan Society for the Promotion of Science KAKENHI grant no. 24KJ1497 to AK

12:15pm CDT

WUM - Assessing the Environmental Impact of Irrigation and Fertilization Practices in Greenhouses Using the Gray Water Footpri
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Agricultural runoff can contaminate surface and groundwater through the accumulation of nitrate-nitrogen (NO3-N) and phosphate-phosphorus (PO4-P). The Gray Water Footprint (GWF) estimates the volume of freshwater required to dilute pollutants to achieve target water quality standards. GWF can be used to compare the environmental impact of agricultural practices, such as irrigation and fertilization. In this project, we estimated and compared the GWF of three irrigation systems and two fertilizer rates in the production of Petunia milliflora F1 (Picobella Pink) in greenhouses. The experiment was a split plot design with two factors: irrigation (overhead, drip irrigation, and subirrigation) and control released fertilizer (CRF) rates (2.1gN-1.26gP-1.68gK or 1.8gN-1.08gP-1.44gK per pot). Plant growth and leachate were measured weekly. GWF was estimated with nitrate or phosphate from the leachate recovered from the containers. Significant differences were observed by irrigation system, but not by CRF rate or the interaction between the factors. In general, GWF (L of water to dilute the pollutant) of phosphate was higher than nitrate. The subirrigation, closed irrigation system had zero GWF because it does not release any leachate. Fertilizer inputs can be reduced while effectively maintaining the quality of petunia in container-production; however, it did not affect pollution rates. In contrast, the choice of irrigation system had a significant effect on nitrate and phosphate runoff rates.
Speakers
AP

Alvaro Pantoja-Benavides

University of Connecticut
Co-authors
RR

Rosa Raudales

University of Connecticut
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

WUM - Comparison of Three Common Brands of Smart Irrigation Controllers to Evaluate Their Water Saving Potential for Sage (Sal
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Improving irrigation efficiency is essential for sustainable agricultural production. Smart irrigation technologies enhance water-use efficiency by integrating wireless communication, advanced sensors, and adaptive scheduling based on actual plant water requirements and weather conditions. Unlike traditional fixed-schedule timers, smart controllers dynamically adjust irrigation to optimize water use. This study aimed to compare three commercially available smart irrigation controllers: Hunter® Hydrawise (Hunter Industries, San Marcos, CA, USA), Orbit® B-hyve (Salt Lake City, UT, USA), and Rachio (Rachio Inc., Denver, CO, USA). The experiment was conducted at the Utah State University Greenville Experiment Station in Logan, Utah, USA. The experimental site (18.5 m x 6.4 m) comprised 12 plots (1.8 m x 1.8 m each), arranged in a completely randomized block design with three blocks, each containing four treatments (three smart controllers and one control). The control plot operated on a fixed timer: 20 minutes of irrigation daily managed by Rachio, whereas the other three smart controllers operated independently utilizing the weather data to schedule irrigation. Each plot was equipped with four sprinkler heads positioned at the corners. Controllers were installed and configured following manufacturer guidelines, utilizing Wi-Fi-enabled communication with their respective software applications. Each controller was configured, ensuring similar settings for a fair comparison, adhering to their respective technical features. Plugs of sage plants were transplanted in 7.5 L containers filled with Metro-Mix® 820 substrate. Initially, plants were irrigated daily for 20 minutes for two weeks to ensure proper establishment. Subsequently, the smart controllers managed irrigation based on real-time weather data, maintaining substrate moisture between 25-30% volumetric water content. Results showed significantly higher total water use in the control plots compared to those managed by smart controllers. Among the smart controllers, Hunter Hydrawise consumed significantly more water than Orbit and Rachio. Growth parameters including plant height, growth index, biomass, and visual appearance did not differ significantly across treatments. However, flower dry weight was significantly higher under Orbit compared to the control and Hydrawise, and similar to Rachio. Physiological parameters such as assimilation rate, stomatal conductance, maximum photochemical efficiency (Fv/Fm), Soil Plant Analysis Development (SPAD), Normalized Difference Vegetation Index (NDVI) remained consistent among all treatments. This study will be replicated during the upcoming summer to further validate the findings and enhance the reliability of the results.
Speakers
AC

Anju Chaudhary

Utah State University
Co-authors
SP

Shital Poudyal

Utah State University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

WUM - Evapotranspiration of Young Pecan Orchard in the Mesilla Valley, NM
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Dona Ana County is one of the largest producers of pecans in the nation, making this area one of the most significant pecan production regions in the world. However, it is facing a shortage of water due to prolonged drought in the region. Management of water to grow crops, including pecan, is crucial to sustaining the agricultural industry in the region. This study assesses the evapotranspiration (ET) of a flood-irrigated young pecan orchard planted in 2021 in the Valley. This orchard has a partial canopy cover of pecan and pasture as a cover crop above the soil. In this orchard, ET was measured using an eddy covariance system and calculated as a residual using the energy budget method. Results indicate that ET primarily varies between 2.5 and 5.5 mm/day from June to August in the years 2021 and 2022.
Speakers
UP

Uduwarage Perera

New Mexico State University
Co-authors
AS

A. Salim Bawazir

New Mexico State University
NA
AF

Alexander Fernald

New Mexico State University
NA
RH

Richard Heerema

New Mexico State University
NA
TK

Thanushan Kirupairaja

New Mexico State University
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Water Utilization and Management

12:15pm CDT

WUM - Greener Landscapes in a Drier World: Identifying Drought-Tolerant Ornamentals Plants in a Changing Climate for Western O
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Climate change, including rising average global temperatures, prolonged drought, and irregular weather patterns, presents significant challenges to landscape plant communities. Urban green spaces are vital to support mental health, mitigate urban heat island effects, and foster community cohesion. The objective of this project is to evaluate the drought-tolerance and ecosystem services of several ornamental plants. We hypothesize that, compared to other species, slow-growing broadleaf deciduous plants will exhibit superior drought resilience by sustaining greater biomass, attracting more pollinators, and preserving aesthetic value in the landscape. The trial was established April 20204, at Oregon State University’s Lewis-Brown Research Station in Corvallis, Oregon on a Chehalis silt clay loam. Experimental design is a randomized complete block with four replications. The single factor is plant taxa, which include 17 unique taxa of monocots, broadleaf deciduous and broadleaf evergreen, and conifer. From May 15, 2024 to Aug 30, 2024 date individual plants received an average of 1 gallon of water per week applied using a 0.5 GPH emitter. Their performance was assessed using leaf area index (LAI), plant height and width, presence of living leaves, pollinator activity, and volumetric water content (VWC). All plants survived the initial establishment in spring of 2024 and subsequent summer irrigation of 0.5 gallons per week per plant. Allium, Muscari armeniacum, and Narcissus exhibited vegetative growth in spring, fall, and winter, and summer dormancy or drought avoidance. Ceanothus, Cornus stolonifera, and Physocarpus opulifolius, being deciduous plants, retained foliage in spring, summer, and fall, and winter dormancy. After the first year, we found that broadleaf deciduous species tolerated drought while increasing in height and width, while enhancing urban green spaces during the spring and summer months. Juniperus squamata, Rosmarinus officinalis, and Arctostaphylos coloradoensis maintained year-round green vegetation. In summer of 2025 no irrigation will be applied, and data will be collated until July and presented at the this conference. After the first year, we found that broadleaf deciduous species tolerated drought while increasing in height and width, while enhancing urban green spaces during the spring and summer months. Findings from this study will provide data-driven recommendations to improve ecological management and guide the landscape industry in selecting climate-adapted species for the Pacific Northwest.
Speakers
CS

Christian Sanchez

Oregon State University
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Water Utilization and Management

12:15pm CDT

WUM - Measuring evapotranspiration rates in commercial broccoli fields - Salinas Valley, CA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Salinas Valley is a major U.S. production region for cool-season vegetables. As regional producers work to achieve groundwater sustainability, there is a growing need to improve irrigation efficiency while sustaining crop yields. Recent advancements and availability in satellite-derived evapotranspiration (ET) data provide opportunities to inform on-farm water management. Quantifying the accuracy and limitations of these methods, however, remains important to build trust for increased operational adoption. This is especially the case for short-season vegetable crops, where performance evaluations of satellite-derived ET have been limited to-date. OpenET is a free, publicly-available platform that uses an ensemble of six satellite-based models to monitor and archive daily-to-monthly ET throughout the western U.S. at 0.25 acre resolution. In this study, daily OpenET values were compared with in-situ ET data from an eddy covariance system deployed in commercial broccoli fields during 2023 (66 days) and 2024 (76 days). Applied water was measured by an on-site digital flow meter, and precipitation was recorded by a nearby weather station. Cumulative totals from the OpenET ensemble mean were within 8.2% and 0.7% of in-situ data during the 2023 and 2024 deployment periods, respectively. Summary performance metrics were within previously published ranges for cropland sites during 2023 (mean bias error: 0.27 mm/day, mean absolute error: 0.65 mm/day) and 2024 (mean bias error: 0.02 mm/day, mean absolute error: 0.61 mm/day). Ensemble ET totals represented 88% of the 344 mm of total water received from irrigation and rainfall for the full crop cycle in 2023 (92 days), and 67% of 518 mm water received during 2024 (101 days). Results indicate OpenET quantified crop water consumption at these two sites with reasonable accuracy, while revealing differences in irrigation application efficiency. Additional discussion will address potential sources of satellite model uncertainty, challenges of collecting eddy covariance data in commercial plantings of short-season horticultural crops, and future verification efforts planned for regional high value specialty crops.
Speakers
MB

Michael Biedebach

California State University, Monterey Bay
Co-authors
AP

Adam Purdy

California State University, Monterey Bay
NA
LJ

Lee Johnson

California State University, Monterey Bay
NA
MC

Michael Cahn

UC Cooperative Extension
RS

Ryan Solymar

California State University, Monterey Bay
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Water Utilization and Management

12:15pm CDT

WUM - The foliar application of silicon on the production agrícola of mini watermelon cv. Sugar baby cause mitigating effects
Thursday July 31, 2025 12:15pm - 1:00pm CDT
The Plants in ambient conditions are subject to dealing with biotic and abiotic stresses. Water deficit, being an abiotic stress, causes changes in plants that make them respond in several ways, such as reduced growth, leaf senescence and lower fruit growth rate, production of Reactive Oxygen Species (ROS), caused by a deficiency in the dissipation of energy due to impaired photosynthesis. The application of silicon becomes an alternative to mitigate the effects of this stress on plants, being deposited in the cell wall, providing rigidity, and increasing the plant's defense enzymes. The study aimed to understand the morphological, physical, and post-harvest responses of mini watermelon according to different soil humidity associated with the foliar application of silicon. The study was conducted in a greenhouse using the mini watermelon cv. Sugar Baby. The experimental design was in randomized blocks, in a 3x2 factorial scheme, with three water tensions in the soil (-35 kPa without water deficit, -50 kPa moderate water deficit, and -65 KPa severe water deficit) and two doses of foliar Si (0 and 1.5 g L-1), with four repetitions. The variables plant length, stem diameter and shoot dry mass, root dry mass, total soluble carbohydrates, proline, gas exchange, and post-harvest analyses were analyzed. There was a significant difference for the variables (p>0.05), but there was no interaction between tension and Si. Proline levels were not statistically significant. The water deficit promoted shorter plant length, aerial part dry mass, root dry mass and Si provided greater stem diameter. For biochemical variables, water deficit caused a higher carbohydrate content in the leaf and lower gas exchange rates. Si influenced skin thickness and average fruit weight. Thus, SI proves to be a strategy for cultivating mini watermelon in conditions of deficient water application.
Speakers
MM

Marcio Mesquita

Federal University of Goiás
Co-authors
HO

Henrique Oliveira

Goiano Federal Institute
MG

Moemy Gomes de Moraes

Federal University of Goias
NA
RB

Rafael Battisti

Federal University of Goias
NA
RA

Ricardo Aparecido dos Santos

Federal University of Goias
NA
RA

Rilner Alves Flores

Federal University of Goias
NA
Thursday July 31, 2025 12:15pm - 1:00pm CDT
Empire AB

1:00pm CDT

CE 1 - Dynamic 24-hour Lighting Can Maintain Winter Greenhouse Strawberry Production While Reducing Lighting Electricity Cost
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Greenhouse strawberry greenhouse production in Ontario, Canada has dramatically increased in recent years. This rise in strawberry production is primarily driven by consumer demand for high quality fruit year-round. To achieve true year-round production, supplemental lighting is needed to compensate for the low sunlight condition during the winter months. Photoperiod extension (up to 24 hour(h)) is a promising strategy which can be implemented in Canada as the utility companies incentivize the use of low cost, off-peak electricity use during the night. Our study aimed to look at the impact of various lighting treatments with photoperiods up to 24h on strawberry growth, production, and fruit quality. Three ever-bearing strawberry cultivars ‘Albion’, ‘Favori’, and ‘Bravura’ were grown in four adjacent double-poly greenhouse at the Harrow Research and Development Centre in Harrow, Ontario, Canada from fall of 2023 to spring 2024. The plants were subject to 4 lighting treatments: 16h of white light (16W), 16h of white light plus far-red (16WFR), 16h of white light followed by 8h of blue light (16W8B), and 16h of white light followed by 8h of blue and far-red light (16W8BFR). Plants under all light treatments were subject to the same DLI. In all strawberry cultivars, plants grown under 16W8BFR produced a similar fruit number and cumulative fruit weight as those grown under 16W and 16WFR. In addition, strawberry fruit produced from plants grown under both 16W8B and 16W8BFR had similar Brix or titratable acidity values when compared to fruit from 16W and 16WFR treatments. In depth physiological responses to extended photoperiods will also be discussed. The results show that in three ever-bearing strawberry cultivars, 24h lighting is a viable production strategy which can reduce the electrical cost associated with lighting while maintaining fruit yield and quality.
Speakers
XH

Xiuming Hao

Agriculture and Agri-Food Canada (AAFC)
Co-authors
CL

Celeste Little

Agriculture and Agri-Food Canada
NA
JL

Jason Lanoue

Agriculture
SS

Sarah St. Louis

Agriculture and Agri-Food Canada
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

CE 1 - Effect of Growth-Promoting Bacteria on Strawberry Yield Under Soilless Greenhouse Conditions
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Bacterial endophytes, which live inside plants, could be beneficial by enhancing plant growth, improving stress tolerance, and combating diseases. In a previous study, greenhouse soil-based strawberries inoculated with endophytes exhibited over 60% more root growth. Field strawberry research demonstrated an average marketable yield increase of 15% from a single endophyte (IALR619) across multiple sites. However, high environment variability, resulted in inconsistent yield performance at these field sites. Since greenhouse hydroponics allows the regulation of nutrients and climate variables, it was hypothesized that the addition of growth-promoting bacteria would result in higher yields. In a pilot run, three bacteria and three day-neutral strawberry cultivars were screened. Based on the preliminary results, two endophytes, IALR1379 and IALR619, were tested on the strawberry cultivar Albion using a randomized complete block design (RCBD) with three replications. IALR619 is mainly a biocontrol bacterium with moderate ability to synthesize plant hormone auxin and solubilize insoluble phosphate compounds. IALR1379 has high activity of auxin synthesis, N-fixing, and solubilization of insoluble phosphates. Bato buckets filled with perlite were planted with strawberry bare roots in the Fall of 2024. Each replication or unit consisted of five Bato buckets connected to a single nutrient reservoir, with four plants per bucket. EC and pH were maintained at 1 ± 0.01 and 6 ± 0.01. The treatments included IALR1379 and IALR619 inoculated plants and a non-inoculated control. First bacterial inoculation was done when new leaves appeared, followed by a second treatment applied one month later. Fruits were harvested weekly over nine instances, and the fresh weight of marketable, unmarketable, and diseased fruits was recorded separately. The quality of the fruits was determined by measuring the BRIX index of 4-5 fully ripe fruits. Chlorophyll content was measured using a SPAD meter. The average marketable yield of IALR619-treated plants was 8128 g, while IALR1379 treated plants and control plants recorded 7474 g and 7071 g, respectively. Although these values were not statistically significant, the yield increase of IALR619-treated plants was 14.9% higher compared to the control. SPAD values and BRIX index for different treatments were not significant. The beneficial effects of endophytes are most evident when plants are under stress. This experiment will be repeated in the spring to determine whether endophytes and can help strawberries to tolerate summer heat and achieve higher yields.
Speakers
BA

Bimal Amaradasa

Institute for Advance Learning and Research
Dr. Sajeewa Amaradasa joined IALR in 2018 as a plant pathologist. His research is focused on investigating beneficial plant bacterial endophytes to control economically important diseases of crops. Dr. Amaradasa’s work involves collecting crop-specific fungal plant pathogens important... Read More →
Co-authors
AT

Amy Turner

The Institute for Advanced Learning and Research
NA
SL

Scott Lowman

The Institute for Advanced Learning and Research
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 1
  • Poster # csv
  • Funding Source The USDA SCBGP grant administered by the Virginia Department of Agriculture and Consumer Services

1:00pm CDT

CE 1 - Genetic Improvement of Fruit and Vegetable Crops for Enhanced Stress Resistance at CPBGG, South Carolina State Universit
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Title: Genetic Improvement of Fruit and Vegetable Crops for Enhanced Stress Resistance at CPBGG, SCSU Bazgha Zia1, Fetien A. Abera1. 1Center of Plant Breeding, Genetics and Genomics (CPBGG), Public Service and Agriculture, South Carolina State University, 300 College Street, Orangeburg, SC 29117. The Center for Plant Breeding, Genetics, and Genomics, established in 2024 at SCSU is dedicated to advancing resilient crops through innovative breeding techniques, genetic research, and genomic technologies. The center focuses on creating resilient, high-yield crop varieties to address global food security challenges, particularly under stressors such as drought, unfavorable temperature conditions. By integrating global partnership, classical plant breeding methods with cutting-edge genomic tools, the center aims to develop superior crop varieties that meet the needs of farmers, consumers, and the agricultural industries Vegetable production faces numerous challenges, including disease pressure, drought, salinity, and extreme temperature conditions, all of which compromise the quality, yield, and nutritional value of vegetables. We invite universities, private companies, and other relevant stakeholders to collaborate with us for 1) Genetic improvement: Leveraging cutting-edge genomic tools and resources to develop crops with superior stress resistance, 2) Testing and demonstration: Partnering in the introduction and evaluation of new rootstocks, varieties, and genetic material for practical application in diverse agroecological conditions and 3) On-farm experimentation: Utilizing both on-station and farmer-partnered farms for real-world testing and genetic characterization of diverse crop genotypes. In alignment with our strategic goals, we introduced CPBGG and conducted a rapid assessment to identify the challenges farmers face in growing various crops, vegetables, and fruits in Law and Orangeburg counties. The assessment involved 65 farmers from young farmers' cooperative associations and small-scale farming communities. Our findings revealed a significant demand for better access to knowledge and technology. In fact, a few farmers even invited us to conduct experiments on their farms. This positive process underscores the importance of the center's ongoing efforts to forge partnerships with universities and private companies. We are introducing watermelon rootstocks to demonstrate and test their performance. Additionally, we are keen to collaborate in genetic improvement, using both the on-station farm and the farms of our committed farmers for the genetic characterization and genetic analysis of diverse genotypes. Through collaborative efforts, we aim to create innovative solutions for small-scale farmers and agro-businesses, improving food security and agricultural resilience.
Speakers
BZ

Bazgha Zia

South Carolina State University
Co-authors
FA

Fetein A. Abera

South Carolina State University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

CE 1 - Interaction of Photoperiod and Temperature on Flowering of Short- and Long-day Strawberry (Fragaria x ananassa) Cultivar
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Strawberry (Fragaria × ananassa) ranks third among fruits produced in the United States and continues to gain popularity among consumers. Its adaptability for controlled-environment agriculture (CEA) based production system makes strawberries an attractive fruit crop for year-round production. However, achieving optimal productivity requires precise management of flowering for fruit yield and suppressing flowers for more runner production for nursery propagation. Therefore, understanding how different cultivars respond to environmental factors such as temperature and photoperiods is essential. This study explored the flowering pattern of two strawberry cultivars, 'Seascape' (long-day) and 'Earliglow' (short-day), under varying temperatures (12°C, 20°C, and 28°C) and photoperiod conditions. In experiment 1, both cultivars were grown in growth chambers at a 16-hour photoperiod for eight weeks. 'Seascape,' despite being a long-day cultivar, did not show flowering under these conditions, while 'Earliglow,' as anticipated, also did not initiate flower due to its short-day requirements. In experiment 2, plants were grown in a growth chamber under a short photoperiod (12 hours) at the same temperature treatments before being transferred to greenhouse conditions (16-hour photoperiod, 22°C). Interestingly, both cultivars previously exposed to 28°C initiated flowering earlier compared to other treatments. Notably, 'Seascape,' despite its classification as a long-day cultivar, demonstrated a clear dependency on the short-day induction period for flower initiation. 'Earliglow' plants exposed to 12°C exhibited delayed flowering. However, these plants developed elongated inflorescences and produced significantly higher numbers of flowers over time than plants exposed to higher temperatures (20°C and 28°C). Further investigation into these phenomena through transcriptomic, metabolomic, and hormonomic analyses is necessary to characterize the underlying regulatory mechanisms of the photoperiod and temperature interaction in strawberry flowering.
Speakers
MH

Mahadi Hasan Redoy

University of Tennessee
Co-authors
EW

Ethan W. Darby

The University of Tennessee
NA
TI

Tabibul Islam

University of Tennessee
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

CE 1 - Light Quality Impacts on Rooting and Growth of Strawberry Cuttings in Controlled Vertical Farming Conditions
Thursday July 31, 2025 1:00pm - 1:45pm CDT
The demand for vigorous strawberry plantlets is increasing to ensure stable production, but challenges in producing disease-free plantlets have emerged due to external environmental changes, including abnormal weather patterns. Additionally, developing a strawberry vertical farm export model is crucial for expanding the global market for high-quality Korean strawberries. To achieve this, optimal nursery technology is required to produce healthy plantlets under stable, disease-free conditions. This study aimed to determine the optimal light quality to enhance the propagation efficiency of strawberry cuttings in a controlled vertical farming system. Strawberry cuttings of the ‘Sulhyang’ cultivar were collected from a greenhouse on December 9, 2024, and transplanted into a vertical farm on December 10. The plants were grown in an environmentally controlled system maintained at 23°C, 70% relative humidity, a 16-hour photoperiod, and a light intensity of 300 μmol·m⁻²·s⁻¹. The medium contained 165 mL of mixed substrates, and 24-cutting trays (500 × 330 × 100 mm) were used. The experiment consisted of four light treatments: Red Blue White (RBW), Red Blue White UV (RBW UV), Red Blue White Far-red (RBW FR), and Red Blue White UV Far-red (RBW UV FR). Growth parameters were measured at 13, 41, and 79 days after transplanting. At 13 days, plantlets under Far-red (FR) light exhibited a 40% increase in shoot length and a 43% increase in petiole length compared to those under UV treatment. Additionally, at 800 μmol·m⁻²·s⁻¹ light intensity, the photosynthetic rate in UV-treated plantlets was 10% higher than in RBW-treated plantlets. At 41 days, the root surface area of UV-treated plantlets was 21% greater than that of RBW-treated plantlets. After 79 days, UV-treated plantlets showed a 69% increase in root strength and a 36% increase in total chlorophyll content compared to RBW treatment. These results suggest that UV treatment in vertical farms can enhance rhizosphere development and physiological capacity, leading to the production of more vigorous strawberry plantlets. This approach may contribute to the expansion of the strawberry vertical farming industry and improve the quality of plantlets for export.
Speakers
SC

Su-Hyun Choi

National Institute of Horticultural and Herbal Science, RDA
Co-authors
DK

Dae-Young Kim

National Institute of Horticultural and Herbal Science, RDA
NA
DL

Dongsoo Lee

National Institute of Horticultural and Herbal Science, RDA
NA
HR

Hee-Ryong Ryu

National Institute of Horticultural and Herbal Science, RDA
NA
HL

Hye-Eun Lee

National Institute of Horticultural and Herbal Science, RDA
NA
KY

Kyeong-Hwan Yeo

National Institute of Horticultural and Herbal Science, RDA
NA
NY

Nari Yu

National Institute of Horticultural and Herbal Science, RDA
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 1
  • Poster # csv
  • Funding Source Rural Development Administration (RDA), Republic of Korea, grant number PJ01752101

1:00pm CDT

CE 1 - Photosynthetically Active Radiation and Leaf Temperature in the Early Development of Strawberry Plants in Vertical Farming
Thursday July 31, 2025 1:00pm - 1:45pm CDT
The study explores the relationship between light intensity and leaf temperature difference in strawberry plants grown using biotechnological tools in a vertical system under artificial full-spectrum lighting (AFSL). The research reveals scientific findings that enhance vertical farming practices and increase the sustainability of these systems. The aim of this project is to identify the light intensity, using LED panels, that provides the highest economic return for strawberry cultivation, correlating energy efficiency, morphological, and physiological characteristics in strawberry production. Increased light intensity and leaf temperature resulted in reduced plant development, lowering gas exchange, photosynthetic rates, and chlorophyll levels.
Speakers
avatar for Henrique Oliveira

Henrique Oliveira

Researcher/Full Professor, Goiano Federal Institute
Co-authors
CM

Cleiton Mateus Sousa

Instituto Federal Goiano
NA
GF

Gabriella Ferreira Goncalves

Goiano Federal Institute
NA
JL

Jhon Lennon Bezerra da Silva

Goiano Federal Institute
NA
MM

Marcio Mesquita

Federal University of Goiás
RS

Rhuanito S. Ferrarezi

University of Georgia
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 1
  • Poster # csv
  • Funding Source National Council for Scientific and Technological Development (CNPq) ; Goiano Federal Institute and Foundation for Research Support of Goiás State (FAPEG)
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

1:00pm CDT

CE 1 - Short-term Carbon Dioxide Enrichment Results in Physiological Acclimation Within One Week for Strawberry
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Carbon dioxide (CO2) is a critical input for photosynthesis and subsequent plant growth. In controlled environments, CO2 can be enriched above concentrations normally found in the ambient air, which has been shown to accelerate production and increase yields of many crop species. To capitalize on these benefits, broad recommendations have been adopted to enrich CO2 at a concentration of 800-1200 μmol·mol–1. However, these initial benefits may not persist throughout crop production due to prohibitive plant acclimation. The purpose of this study was to determine the timing and severity of CO2 acclimation responses of everbearing strawberry (Fragaria ×ananassa) during the vegetative growth stage. Crown divisions of strawberry ‘Quinault’ were transplanted into 10.7-cm pots and grown for two weeks in walk-in growth chambers under either an ambient (450 μmol·mol–1) or enriched (900 μmol·mol–1) CO2 concentration. Temperature, relative humidity, and photosynthetic photon flux density (PPFD) setpoints were consistent between CO2 treatments at 22/18 °C, 55/65% (day/night), and 320 µmol∙m–2∙s–1 (18-h photoperiod; daily light integral of 21 mol∙m–2∙d–1), respectively. Net photosynthesis (Anet) was surveyed daily for seven days on the same leaf using a portable leaf photosynthesis system, and A in response to internal leaf CO2 concentration (Ci) (A-Ci) curves were collected on day seven. On day 14, Anet and A-Ci curves were collected on the same leaf and on the most recently expanded new leaf, before destructive data (e.g., leaf area, dry mass) were collected. Survey measurements revealed Anet was higher for plants grown under the enriched compared to ambient CO2 concentration across all seven days. However, Anet declined after day 3 for enriched plants compared to relatively stable Anet measurements for ambient plants. After one week of exposure under treatment conditions, maximum rate of photosynthetic electron transport (Jmax) and maximum rate of Rubisco carboxylase (Vcmax) (both indicators of photosynthetic acclimation) were lower under the enriched compared to ambient CO2 concentration. No difference in dry mass between treatments was observed. While the higher Anet observed under the enriched CO2 concentration indicates a potential benefit for production, declining Anet after three days and physiological acclimation within one week pose possible limitations to maximizing the use of this input. Further elucidating the timing of strawberry acclimation to CO2 enrichment will help to improve control strategies for controlled environments to optimize plant yield and quality with fewer resources.
Speakers
JC

Joshua Craver

Colorado State University
Co-authors
JB

Jennifer Boldt

United States Department of Agriculture
KH

Kale Harbick

United States Department of Agriculture
NA
SP

Samantha P. Rosado

Colorado State University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 1
  • Poster # csv
  • Funding Source USDA-NIFA-UIE Project #: 2023-70019-39371

1:00pm CDT

CE 1 - Strawberry 'Keepsake' in a greenhouse: Improving Yield, Morphology, and Photosystem Efficiency by Optimizing Far-Red Lig
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Far-red (FR) light significantly influences plant growth and development by affecting photosynthesis efficiency, photomorphogenesis, and reproductive characteristics. This study analyzed the effects of four supplemental FR light intensities (90, 60, 30, and 0 µmol/m²/s) with a peak wavelength of 35nm on the growth, yield, and physiological responses of the strawberry (Fragaria x ananassa) cultivar 'Keepsake' in a greenhouse setting under natural daylight. The study's findings indicate that elevated FR intensities (FR90 and FR60) promoted vegetative growth, as evidenced by elongated petioles, increased leaf area, and crown count. The increased intensity of FR light (FR90 and FR60) diminished the efficiency of Photosystem II (PSII) compared to FR0, indicating a potential disruption in PSII functionality, as non-regulated energy loss (PhiNO) was elevated in FR60 and FR90. Despite these physiological changes, the biomass of plants, as well as fruit diameter and weight, remained unaffected across all treatments. However, the net assimilation rate (NAR; grams of dry shoot biomass per cm² leaf area) was higher in FR60 compared to other treatments. In FR30, flowering was postponed by 51 days compared to other treatments, which correlated with the lowest fruit count, while FR90 and FR60 promoted early flowering with a higher fruit count, with FR60 plants reaching uniform flowering sooner than other FR treatments. The total sugar content (TSS) was marginally reduced in FR0. This study reveals that moderate FR intensity positively influences growth and yield, whereas low FR intensity may delay reproduction and overall yield in certain June-bearing strawberry varieties.
Speakers
FK

Fraidoon Karimi

University of Wyoming
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 1
  • Poster # csv
  • Funding Source Presidential Fellowship 2023/24, University of Wyoming

1:00pm CDT

LOCSY - Soil, Media, and Tissue Testing for Urban Farms: Extension Outreach and Discovery in the Unique Urban Growing Systems of Cook County
Thursday July 31, 2025 1:00pm - 1:45pm CDT
There has been a long tradition of Cooperative Extension as a "go-to" source for soil testing in the popular media and research literature. However, soil and media testing within the unique growing systems of urban agriculture has not been extensively explored. In 2021, Cook County Extension began directly subsidizing and conducting multiple soil, compost, media, and tissue tests for a cohort of urban farms in the greater Chicagoland area. Using the data collected, we began building a baseline soil test database to help growers make specific nutrient and media management decisions. Through soil data collection and interpretation, we hoped to begin reducing the guesswork and decision-making of which tests to take, when, and how to interpret them. The standard soil, media, and tissue testing results reveal important soil, media, and uptake trends but are not always straightforward to interpret. Continued work with testing protocols, media incubation studies, and field trials could reveal more predictable trends in the future to better guide urban farmers' nutrient management planning. This poster presents the initial soil database results and interpretation to begin to understand and help explain the unique growing conditions of urban agriculture.
Speakers
ZG

Zachary Grant

Extension Educator, University of Illinois Extension
Zack is a Local Foods and Small Farms Extension Educator housed in Cook County, Unit 6.  He joined Extension in 2015 to focus on urban agriculture production programming for a diverse group of stakeholders. Zack educates, conducts research, and provides technical assistance in small... Read More →
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Local Food Systems

1:00pm CDT

PH 2 - Analyzing Aflatoxin Risks Through Molecular Detection of A. flavus Strains and Co-Infection Patterns In Peanut Seeds
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Aspergillus flavus is an airborne fungus that can contaminate agricultural commodities very rapidly, including grains and nuts. This pathogen causes aflatoxin contamination in peanuts, which leads to significant crop losses and their quality, particularly in storage conditions. Accurately identifying toxin and non-toxin producing A. flavus isolates is important in terms of effective management because traditional approaches have limitations such as being less effective and time-consuming. To address this problem, we developed molecular tools to differentiate toxigenic and non-toxigenic A. flavus isolates. We identified A. flavus isolates through morphological and species-specific primer (FLA1/FLA2). Also, to characterize toxigenic and non-toxigenic A. flavus isolates, we employed a combination of approaches such as RT-PCR, RT-qPCR, and aflatoxin measurement, focusing on four genes (AflD, AflQ, AflP, AflR) from the aflatoxin biosynthesis gene cluster with primer optimization. In addition, research on the co-infection pattern of aflatoxin contamination on peanut seeds during storage conditions is limited. To address this, we collected peanut isolates from different seed lots and performed ITS sequencing to understand the pattern of microbial communities on peanut seeds during aflatoxin contamination. Regarding aflatoxigenic and non-toxigenic A. flavus detection, different gene expression patterns were observed among four AFs biosynthesis genes. However, by combining gene expression patterns, quantification and AFs production, we differentiated toxin and non-toxin producing A. flavus isolates. These results indicated the efficacy and specificity of these molecular tools, which could be helpful for developing good molecular markers for distinguishing toxigenic and atoxigenic isolates and to manage A. flavus contamination in peanut seed lots. Our co-infection results from peanut seed lots during multiple years observed the infection of diverse microbiota along with A. flavus, which indicates other microbes co-infect with A. flavus infection. This co-infection could trigger aflatoxin production in peanut seeds. Therefore, this approach offers novel insights into peanut seed-associated microbiomes, co-infection and aflatoxin production, shedding light on the correlation between the role of microbial communities and aflatoxin contamination.
Speakers
MM

Md Mostafa Masud

Alcorn State University
Co-authors
EA

Emran Ali

Alcorn State University
NA
SW

Sumyya Waliullah

Alcorn State University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2

1:00pm CDT

PH 2 - Assessing Postharvest Texture Variations in Rabbiteye and Southern Highbush Blueberries Grown in Alabama
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Blueberry firmness is a crucial factor affecting shelf-life, fruit quality and consumer acceptance. Rabbiteye (RE; Vaccinium virgatum) blueberries are noted for their tough skin and lower pulp firmness compared to southern highbush (SHB; Vaccinium corymbosum L.). Yet limited firmness data is available on newly released RE genotypes bred for high yield, large berry size and increased firmness. The research objective was to evaluate weight loss, fruit firmness, and berry diameter on 14 RE and 3 SHB genotypes through storage. Blueberries were harvested from E.V. Smith Research station (Tallassee, AL) in 2024, then stored at 4 °C and 85% relative humidity. Four postharvest timepoints were followed: days 0, 14, 28, and 42 in storage. Firmness and fruit size were measured using a FruitFirm1000 texture analyzer, and weight loss was recorded using a digital scale. Significant changes in weight loss (%), firmness (g/mm), and fruit size (mm) during storage were found for the interaction between genotype and days in storage. On day 42, RE genotypes of ‘T-3081’, ‘Vernon’, and ‘T-3075’ exhibited the lowest weight loss at 7.2%, 7.8%, and 7.9%, respectively. Conversely, ‘MS1228R’ (18.3%), ‘MS1110R’ (13.6%), and ‘Titan’ (13.2%) (RE genotypes) and ‘Newhanover’ (14.9%) (SHB genotype) had the highest weight loss. During storage, most cultivars decreased in berry diameter. RE genotypes ‘Titan’, ‘T-3081’, and ‘T-3075’ had the largest diameter (24.9, 24.1, and 26.1 mm, respectively) at harvest and retained fruit diameter through storage (23.7, 23.4, and 26.1 mm, respectively). At harvest (day 0), ‘Titan’ (RE) had the highest firmness (287 g/mm), while ‘MS1110R’ (RE) had the lowest (152 g/mm). After 6 weeks of storage (day 42), RE genotypes of ‘Titan’, ‘Vernon’, ‘T-3075’, and SHB ‘Legacy’ maintained high firmness (˃200 g/mm), while ‘Alapaha’ (RE), ‘NewHanover’(SHB), and ‘MS1110R’ (RE) were lowest (˂150 g/mm). Hierarchical Cluster Analysis (HCA) identified three distinct firmness clusters, categorizing cultivars into enhanced (‘Titan’, ‘T-3075’), moderate (‘Legacy’, ‘Overtime’, and ‘T-3081’), and suppressed firmness (‘Alapaha’, ‘Krewer’, ‘Brightwell’, and ‘MS1110R’). HCA was crucial in selecting 10 genotypes (7 RE and 3 SHB) for future cell wall and texture analysis. This study provided valuable data for growers to select RE genotypes with maintained firmness and shelf-life. This data will aid blueberry breeders in selecting high-quality and competitive RE genotypes.
Speakers
PE

Peter Ephraim

Graduate Research Assistant, Auburn University
Peter Ephraim is a PhD student in the Department of Horticulture at Auburn University. Peter has a strong background in food science, holding a bachelor’s degree in food science and technology from Lilongwe University of Agriculture and Natural Resources in Malawi and a Master’s... Read More →
Co-authors
CR

Camila Rodrigues

Auburn University
NA
MT

Marlee Trandel Hayse

Auburn University
MM

Michael Miller

Auburn University
NA
SR

Sushan Ru

Auburn University
SJ

Suzanne Johanningsmeier

United States Department of Agriculture-Agricultural Research Service (USDA-ARS)
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2
  • Subject Postharvest
  • Funding Source Southern Region Small Fruit Consortium

1:00pm CDT

PH 2 - Assessing Shelf-life and Nutritional Value of Vitamin C Biofortified Lettuce
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Enhancing vitamin C concentration in crops like lettuce (Lactuca sativa L.) through biofortification can increase dietary intake for consumers, yet its rapid degradation post-harvest remains a challenge. This limitation necessitates practical strategies to optimize post-harvest retention of ascorbic acid and extend the shelf-life of lettuce. This study aims to assess the effect of ascorbic acid foliar sprays on post-harvest stability of vitamin C content during post-harvest storage. A shelf-life study was conducted under refrigerated (4°C) and ambient (22°C) storage conditions over a 72-hour period. Potential results suggest that higher vitamin C concentrations may enhance leaf chlorophyll content and antioxidant capacity, potentially improving overall plant health and stress resistance. However, increased vitamin C levels could also lead to greater oxidation over time, impacting nutrient retention post-harvest stability. This study is expected to demonstrate the feasibility of vitamin C biofortification as a functional food strategy, enhancing the nutritional profile of lettuce while maintaining acceptable post-harvest quality. The findings from this study could provide valuable insights into improving fresh produce shelf-life and micronutrient retention. This research hopes to address dietary deficiencies while minimizing food waste.
Speakers
KM

Katerina Mojica Vargas

Graduate Research Assistant, Texas Tech University
Co-authors
CS

Catherine Simpson

Texas Tech University
HL

Haydee Laza

Texas Tech University
NA
LT

Leslie Thompson

Texas Tech University
NA
SS

Sukhbir Singh

Texas Tech University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2
  • Poster # csv

1:00pm CDT

PH 2 - Comparison of Fruit Quality, Anthocyanin Contents, and Volatile Organic Compounds at Various Pigmentation Levels in Red
Thursday July 31, 2025 1:00pm - 1:45pm CDT
As consumer preferences and needs for strawberries have become increasingly diverse, various strawberry cultivars have been developed in Korea. Although the ‘Seolhyang’ (SH) cultivar still occupies the highest proportion in the strawberry industry, the proportion of strawberry cultivars has unique characteristics, including bigger size, higher ascorbic acid content, or unique color. One of the distinctive cultivars recently gaining attention is a white-pink colored strawberry known as ‘Cinderella’ (CD). Thus, the objective of this study aimed to evaluate the metabolic variations in secondary metabolites between SH and CD cultivars, based on the pigmentation of the fruit peel, which could be 0%, 50%, 75%, or 100%, following a 3-day cold storage period. The fully developed strawberry had a higher fresh weight and fruit diameter in CD than in SH cultivar. The peel color variables, including lightness, yellowness, and hue angle, were gradually decreased with fruit pigmentation. The redness of the peel increased rapidly along with fruit pigmentation in SH, while that of CD showed only a slight increase. The ethylene production rate indicated higher values in SH than in CD during fruit pigmentation. Fruit firmness gradually decreased while soluble solids content (SSC) increased along with fruit pigmentation, regardless of strawberry cultivars. The anthocyanin analysis revealed that the SH cultivar had notable levels of pelargonidin-3-O-glucoside, pelargonidin-3-(6”-malonylglucoside), and cyanidin-3-O-glucoside, which contributed to fruit pigmentation. In contrast, pelargonidin-3-O-glucoside was only detected in the CD cultivar. Based on the volatile organic compounds (VOCs) profiling, a total of 74 VOCs were detected, including 26 esters, 14 alcohols, 12 aldehydes, 9 ketones, 5 terpenes, and 3 acids. In SH cultivar, (E)-2-hexenal, (E)-nerolidol, methyl hexanoate, hexanoic acid, and linalool were major VOCs. On the other hand, methyl hexanoate, ethyl hexanoate, (E)-2-hexenal, mesifurane, and (E)-nerolidol were the main VOCs in the CD cultivar. Therefore, the results indicate that the profiles and levels of anthocyanins and VOCs were differently regulated during the pigmentation of strawberry fruit between SH and CD cultivars.
Speakers
avatar for Hyowon Park

Hyowon Park

Ph.D candidate, Chung-Ang University
Co-authors
HK

Ho-Yeon Kim

Korea Institute of Science and Technology
NA
JL

Jinwook Lee

Chung-Ang University
NA
JP

Jiwon Park

Chung-Ang University
NA
JJ

Joohyuk Jo

Chung-Ang University
NA
SB

Si-Eun Byeon

Chung-Ang University
NA
TL

Theintthandar Latt

Chung Ang University
PhD candidate at Postharvest Physiology Laboratory, Chung-Ang University.
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2
  • Subject Postharvest
  • Funding Source This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through ‘Smart Agri Products Flow Storage Technology Development Program’ funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA; Grant No. 322052052HD030).

1:00pm CDT

PH 2 - Degreening temperature can be lower for two seedless mandarin (Citrus reticulata Blanco) cultivars
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Production of mandarin, sweet orange, and grapefruit has increased in most tropical and subtropical areas of the world in recent years due to growing market demand for high-quality, blemish-free fruit. Citrus fruit grown in the coastal region of the southeastern U.S. are more susceptible to blemishes and poor peel color than those grown in cooler climates. Early season, Florida mandarins are commonly degreened by treating with ≤ 5 ppm of ethylene for 12 h to 72 h at 29 °C /96% relative humidity (RH). However, optimal citrus degreening conditions vary with citrus cultivar, growing area, stage of maturity and season. Additionally, degreening can negatively affect mandarin quality (flavor, nutrition, acidity, sensory acceptability) and promote postharvest decays. The objective of this study was to evaluate the postharvest quality of two seedless mandarin cultivars degreened at temperatures lower than the commercial standard of 29 °C. These cultivars were released by the University of Florida breeding program: ‘UFGlow’, grown on ‘Rich’ trifoliate, ‘Carrizo’ citrange or ‘Sun Chu Sha’ mandarin rootstocks, and ‘UFSunrise’, grown on ‘Rich trifoliate’, ‘Carrizo citrange’ or HRS 812 rootstocks and were compared to the commercial cultivar ‘Owari-satsuma’, grown on ‘Carrizo citrange’ rootstock. On the day of harvest, fruit of each cultivar were pooled, treated with 5.0 μL·L-1 ethylene in a humidified, flow-through system for 72 hr at 20, 25 or 29 °C and 95% RH, then transferred to 8 °C/87% RH and evaluated weekly for 21 days. The three cultivars had better peel color and higher peel total carotenoid content when degreened at 25 or 29 °C than at 20 °C. Soluble solids content and total titratable acidity changed slightly during storage, independent of degreening treatment. Degreening did not affect ascorbic acid content; fruit total antioxidant capacity (oxygen radical absorbance capacity, ORAC) declined less with the ethylene treatment but remained constant in ‘UFGlow’. The best degreening treatment for these seedless mandarin cultivars was 5 ppm ethylene at 25 °C/96% RH for 72 hr, resulting in the best peel color, highest total peel carotenoids content, less weight loss and firmer fruit. Although ‘Owari’-satsuma developed better color at 29 °C, weight loss was significantly higher than when degreened at 20 or 25 °C.
Speakers
SS

Steven Sargent

University of Florida
Professor and Extension Postharvest Specialist
Co-authors
CA

Charles A. Sims

University of Florida/IFAS
NA
JX

Jose X. Chaparro

University of Florida/IFAS
NA
MA

Mark A. Ritenour

University of Florida/IFAS
RO

Ramadhani O. Majubwa

Sokoine University of Agriculture
NA
TM

Theodosy Msogoya

Sokoine University of Agriculture
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2

1:00pm CDT

PH 2 - Effectiveness of Blue Light Over Infrared for Sugar Estimation in Frozen Sweetcorn
Thursday July 31, 2025 1:00pm - 1:45pm CDT
We had earlier showed that spectrometric scanning of dehusked, fresh sweetcorn-on-the-cob (SOTC) in the visual (Vis) blue wavelength range (Vis-blue) offered an alternative nondestructive brix quantitation. In this report, we demonstrate that scanning in the Vis-blue is effective in a wide range of sample temperatures including freezing temperatures where the in-market infrared (IR) scanners fail. This is an important development for instore consumer decision-making experience. Eight packs of frozen (~-340C) SOTC in thin, translucent polyethylene packing bags were evaluated. Each pack contained at least12 pieces of SOTC, representing three different commercial brands bought directly from two different groceries stores on St. Croix. The samples were transported in ice and kept frozen (at temperature conditions as in the stores) until the time of measurements. At least six random SOTC were marked for IR and Vis-blue data every 5 minutes from freezing to room temperatures (~22degC) until the samples were completely thawed. The thawing temperatures at which each instrument was able to start recording were noted. The IR was insensitive at temperatures below ~160C, while the Vis-blue scanner recoded brix on frozen samples all the way to room temperatures and at complete sample thaw. The Vis-blue scanner also measured all frozen samples through the plastic packaging and directly on samples taken out of the packing. The Vis-blue scanner was also able to obtain spectral signals on the SOTC while the samples were in the freezer, but the signals were weak and, in some cases, when the sample packaging was completely covered in ice. With further developments and refinements, the Vis-blue scanning method can be integrated into scanning devices for quality assessments of frozen corn.
Speakers
HA

Henry Awika

University of the Virgin Islands
Co-authors
DG

Donna Gonzalez

University of the Virgin Islandss
NA
JS

Johnson Southwell

University of the Virgin Islandss
NA
TS

Thalia Stanley

University of the Virgin Islands
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2
  • Subject Postharvest
  • Funding Source USDA-AMS-SCBR-VIDA

1:00pm CDT

PH 2 - Maturity of Select Florida Avocado Genotypes for Optimum Eating Quality
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Avocado (Persea americana Mill.) is a subtropical fruit prized for its nutritional properties, versatility, and health benefits. Florida growers have shown interest in expanding avocado production into South-Central Florida due to the decline in citrus production resulting from greening and canker diseases. However, the worldwide market is mostly dominated by the ‘Hass’ cultivar, a Guatemalan (G) x Mexican (M) avocado hybrid with excellent eating quality and the ability to withstand long-distance shipping and postharvest handling. Florida focuses on larger green West-Indian (WI) or WI x G avocado hybrids, suited to subtropical climates. There is a need to identify WI genotypes that are well-adapted to South Central Florida conditions and evaluate their production and quality potential in this region. But the relationship between genotype and maturity is not yet fully understood. This study focuses on determining when these should be harvested to reach full ripeness and achieve the best eating quality, through correlation between dry matter (DM), firmness, and taste preference. A taste panel consisting of 25 panelists evaluated the sensory attributes of avocados that reached firmness equal to 15 N or lower. The results show that WI varieties and their hybrids, despite both low and high DM content (16.4% to 28.4%), are well-accepted by the panelists with taste ratings ranging from 5 (neither like nor dislike) to 6.6 (like moderately). The characteristics that contributed to the panelists' preference include buttery, creamy, nutty, and sweet. Additionally, fruits harvested later tended to show an increase in DM and a decrease in ripening time. Fruits, when harvested at their full physiological maturity, require 10 days to reach full ripeness at room temperature (20°C - 22°C). This study contributes to understanding factors that can help optimize fruit quality and provide valuable insights for the Florida avocado industry.
Speakers
RN

Rachel Noma

UNIVERSITY OF FLORIDA
I am an Agronomist who graduated from Sao Paulo State University. I have experience in fruit production, figs, citrus, lychee, and strawberries, I assisted in data collection for various projects focusing on evaluation of external and internal quality of fruits, fruit quality and... Read More →
Co-authors
AP

Anne Plotto

USDA U.S. Horticultural Research Lab
NA
MR

Mark Ritenour

UNIVERSITY OF FLORIDA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2
  • Poster # csv

1:00pm CDT

PH 2 - Postharvest Assessment of Rabbiteye and Southern Highbush Blueberry Visual and Fruit Quality
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Blueberries are valued for their flavor and nutritional content, yet postharvest data on rabbiteye (RE; V. virgatum A.) genotypes remain limited. In this study fruit quality of 16 RE and 3 southern highbush (SHB; V. corymbosum L.) genotypes were evaluated through cold storage. Fruit harvested in 2024 from E.V. Smith Research Center (Tallassee, AL) were stored at 4°C and 85% relative humidity in a randomized complete block design. Measurements were taken on days (d) 0, 14, 28, and 42 including color attributes (L*, a*, b*, C*, h* CIELAB units), soluble solids content (SSC, °Brix), titratable acidity (Tacid) and pH. Total anthocyanins (mg/g), phenolics (mg/100g), and DPPH antioxidant capacity (mM/100g) were quantitated via UV-VIS spectrophotometry. Significant interaction for days in storage*genotype were observed in Tacid and SSC:Tacid ratio. Tacid was highest in ‘MS1228R’ and ‘MS1234R’ (42d; 0.73 and 14d; 0.77) and lowest in ‘Legacy’ and ‘NC5303’ (42d; 0.30 and 0d; 0.31). The SSC:Tacid ratio was highest in ‘Brightwell’ (0d; 47.47) and lowest in ‘Vernon’ (0d; 19.76). SSC decreased with increased storage time (0d to 28d) by 0.69 °Brix while pH increased from 0d (3.31) to 42d (3.54). Genotype differences indicated ‘Brightwell’ and ‘Ochlocknee’ had the highest SSC (16.5 and 15.9 °Brix) compared to ‘T-3075’ and ‘New Hanover’ (12.4 and 12.1 °Brix). pH was highest in ‘Legacy’ and ‘New Hanover’ (3.67 and 3.63) and lowest in ‘T-3075’ and ‘MS1234R’ (3.25 and 3.19). Across all storage timepoints, RE genotypes were higher in total anthocyanins, phenolics and antioxidant activity at 16.54 mg/g, 298.40 mg/100g and 21.41 mM/100g, respectively compared to SHB genotypes at 11.35 mg/g, 213.90 mg/100g and 17.19 mM/100g. Hierarchical cluster analysis (HCA) indicated 3 clusters of interest. In cluster I, ‘Vernon’, ‘Overtime’ and ‘MS1595R’ had enhanced C* and h*. In cluster II, ‘Brightwell’ and ‘Ochlocknee’ indicated enhanced anthocyanin, phenolic and antioxidant activity, while ‘T-3075’ and ‘T-3072’ had suppressed phytonutrient content in cluster III. The industry accepted range for blueberry SSC is 11.2-14.3 °Brix and all genotypes in this study met industry standards. Some RE selections, e.g., MS selections, were above the U.S. accepted range (0.2 to 0.5) for Tacid relating to sour/tart flavors. HCA was useful in sub-selecting RE and SHB genotypes for sensory and electronic analysis to assess flavor patterns. Identifying fruit quality and flavor traits postharvest will aid in selecting superior RE germplasm to enhance consumer acceptance and inform breeding efforts.
Speakers
CC

Clarisse Cochran-Chipura

Auburn University
Co-authors
EC

Elina Coneva

Auburn University
MT

Marlee Trandel Hayse

Auburn University
SC

Sungeun Cho

Auburn University
NA
SR

Sushan Ru

Auburn University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2
  • Subject Postharvest
  • Funding Source AgR Seed Grant Program # 7008151

1:00pm CDT

PH 2 - The Effects of UV-Treated Hydroponic Nutrient Solution on Postharvest Quality of Romaine Lettuce
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Romaine lettuce (Lactuca sativa L. var. longifolia) is a highly nutritious, leafy green vegetable that can be easily grown hydroponically, offering a sustainable and efficient alternative to conventional agricultural practices. Although hydroponically grown lettuce is less susceptible to microbial infection compared to its soil-grown counterparts, contamination with human pathogens is still possible. Sterilization methods, such as ultraviolet (UV) light treatment, may be employed to control contamination in the hydroponic system, but its effect on lettuce postharvest quality remain unclear. The aim of this study was to investigate the impact of nutrient solutions treated with two doses of UV-C on the harvest and storage quality of the romaine lettuce cultivar 'Sparx' grown in a deep-water culture (DWC) hydroponic system. Nutrient solutions were either left untreated (control) or treated three times over a six-week growing period with UV-C (254 nm) at two different flow rates, delivering estimated UV-C doses of 18.93 mJ/cm² (low) or 37.85 mJ/cm² (high) per treatment. Postharvest quality was evaluated by measuring leaf length, fresh weight, dry root weight, water loss, soil plant analysis development (SPAD), color, texture, visual quality, respiration rate, micronutrient content, and nutritional content at time of harvest and over a seven-day storage period. We utilized a randomized complete block design, and the experiment was repeated three times. Lettuce grown in the low dose UV-C-treated nutrient solution had significantly longer lettuce leaf length, higher fresh weight, and higher vitamin C content compared to the high dose UV-C treatment. Additionally, fresh shoot weight was significantly higher in the low dose UV-C-treated lettuce compared to the high dose treatment. In terms of visual quality, the high dose UV-C treatment showed significantly better visual quality at the end of storage compared to lettuce grown in the control nutrient solution. No significant differences were observed for dry root weight, water loss, SPAD, texture, color, or micronutrient content between the three treatment groups. Overall, this study demonstrates that UV-C-treated nutrient solution had no negative impact on quality parameters of hydroponic lettuce at harvest or during storage, indicating that UV-C treatment, particularly at lower doses, may be a viable option for effectively sterilizing hydroponic systems without compromising lettuce postharvest quality.
Speakers
TK

Tori Kent

Kansas State University
Co-authors
EP

Eleni Pliakoni

Kansas State University
MB

Manreet Bhullar

Kansas State University
SA

Sachin Adhikari

Kansas State University
NA
TJ

Tricia Jenkins

Kansas State University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Postharvest 2
  • Subject Postharvest
  • Funding Source Kansas State University Global Food Systems Seed Grant Program

1:00pm CDT

PROP - Effect of Cellulase, Gibberellins, and Cytokinin on Seed Germination of Rosa arkansana Porter
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Roses seeds (achenes) have a reputation of being challenging to germinate due to physiological and minor physical dormancy. The effect of gibberellins (GA) on rose seed germination has generally been neutral or negligible. Soaking rose seeds in cellulase before stratification has been documented to improve germination in some roses, likely due to degrading the outer covering around the embryo to allow for better imbibition. This experiment uniquely combines cellulase with GA to ascertain if there would be a stronger effect of GA if reaching the embryo was a limitation. A full factorial was used with a two-day duration of either dry seed, a 0.5% cellulase soak, or reverse osmosis (RO) water soak followed by a one-day soak of 200ppm GA3, 200ppm GA4 7, 200ppm GA4 7 plus 6-benzyladenine as Promalin®, or RO water. Rosa arkansana was chosen due to it being challenging to germinate. Four replications of 100 seeds each for each of the 12 treatments were prepared and mixed with 80cc of moist peat moss. All treatments were given a common warm stratification (8 weeks room temperature) and cold stratification (12 weeks 4C) followed by constant 10C in a growth chamber. Germination was recorded and germinated seedlings removed three times while at 10C at 5-week intervals. Promalin® led to slightly faster germination at week 5, but by week 10 GA treatments were not significantly different than the water control. Cellulase and RO water pre-GA soaks were not significantly different than each other at any time interval and by week 15 both were significantly better than the dry seed treatment. This data suggests the importance of having well hydrated seeds going into stratification, a neutral effect of GA on germination of this species, suggests 6-benzyladenine may slightly speed along germination, and that cellulase may not significantly improve water penetration over a sufficient imbibition duration.
Speakers
SM

Sonja Maki

University of Wisconsin-River Falls
Co-authors
DZ

David Zlesak

University of WI River Falls
GO

Gabe Olson-Jensen

University of Wisconsin-River Falls
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Propagation

1:00pm CDT

PROP - Optimizing Micropropagation of Native Mirabilis Species for Sustainable Cultivation
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Native four o’clocks (Mirabilis spp.) are drought-tolerant perennials native to North America, valued for their vibrant flowers and ecological contributions, including pollinator support and soil stabilization. Despite their ornamental appeal and value in ecological restoration, their use in horticulture has been limited due to propagation challenges such as low seed availability, poor germination, and complex dormancy requirements. Micropropagation, the production of new plants from small tissue sections under sterile conditions using artificial media and controlled environments, presents a promising solution to these limitations. It offers a reliable method to produce large quantities of native Mirabilis for conservation and nursery applications. In 2021, an effective micropropagation protocol was developed for the rare North American species Mirabilis macfarlanei. Building on this success, the current project evaluated the applicability of this protocol to three additional native species: Mirabilis greenei, Mirabilis laevis var. villosa, and Mirabilis laevis var. crassifolia. Single-node cuttings were surface sterilized with 20% bleach and cultured on Driver and Kuniyuki Walnut (DKW) medium supplemented with 4 µM 6-benzylaminopurine (BA). All three species were successfully established in vitro and proliferated. Growth parameters, including average number of shoots, shoot length, and shoot dry weight, were compared between DKW with 4 µM BA and Murashige and Skoog (MS) medium with the same BA concentration. For M. greenei, shoot proliferation was similar between MS and DKW (5.7 and 5.8 shoots, respectively). M. laevis var. villosa and M. laevis var. crassifolia produced more shoots on MS medium (10.6 and 4.5) than on DKW (7.8 and 3.9). All species exhibited higher shoot dry weights on MS medium, while shoot lengths were comparable across treatments. Rooting success varied among species. The rooting medium developed for M. macfarlanei (DKW supplemented with 0.5 mg/L indole-3-acetic acid [IAA], 0.5 mg/L naphthaleneacetic acid [NAA], and 0.15 mg/L Sequestrene® 138) was effective only for M. greenei. However, M. laevis var. villosa and M. laevis var. crassifolia rooted better on MS medium containing 1 µM NAA and 0.15 mg/L Sequestrene® 138. Rooted plantlets of all three species were successfully acclimatized in rockwool, with M. laevis var. villosa and M. laevis var. crassifolia flowering under greenhouse conditions.
Speakers
SU

Senay Ugur

University of Idaho
Co-authors
AK

Anne Knerr

University of Idaho
NA
MG

Melissa Galindo

University of Idaho
NA
TP

Timothy Prather

University of Idaho
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Propagation

1:00pm CDT

PROP - Physiological Quality Assessment of Bush Bean (Phaseolus vulgaris) Seeds Using Controlled Deterioration Technique
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Bush bean is a popular vegetable as it is high in fiber, vitamins, minerals, and antioxidants. Assessment of the physiological quality of bush bean seeds is essential for ensuring better crop performance and yield, and the controlled deterioration technique is a reliable and practical tool for this purpose. The objective of this study was to determine the seed quality of two bush bean varieties using the controlled deterioration technique. Two bush bean varieties (Jhar Sheem-1 and Jhar Sheem-2) were induced with 12%, 16%, and 20% moisture levels for 0, 4, 8, and 16 days. Seed quality factors, such as, germination percentage, abnormal seedlings percentage, dead seed percentage, soil emergence percentage, root and shoot lengths, and dry matter content, were evaluated. In this study, 24 treatments were arranged as completely randomized design with 3 replications. Both varieties showed an identical performance at 12% moisture in 0 days of the aging period, but prominent differences were noticed at a highly deteriorated level of the factors. The percentage of normal seedlings declined to 85.34% in Treatment-24 (Jhar Sheem-2 at 20% moisture level with a 16-day aging period) compared to Treatment-1 (Jhar Sheem-1 at 12% moisture level with no aging period). Meanwhile, seedling abnormality increased by 22.00% in Treatment-11 (Jhar Sheem-1 at 20% moisture level with an 8-day aging period) relative to Treatment-1. Conversely, root and shoot dry matter were 93.06% and 28.62% lower, respectively, in Treatment-24 than Treatment-1. Moreover, highly remarkable (1%) relations were traced among all the traits, ranging from -0.466 (seed dry matter x abnormal seedling) to 0.983 (normal seedling x root length). Another finding was that among all the values about normal seedlings (i.e., the germination percentage); their affiliation with the soil emergence was prominent, i.e., 0.983. Thus, the outcomes revealed that the controlled deterioration test was highly effective in assessing the physiological conditions of the varieties. According to the vigor and quality performance, bush bean Variety-1 (Jhar Sheem-1) can be used as a parent for hybridization programs to produce superior inherent quality. Future investigations can be conducted to find more acceptable temperature and moisture trait limits among other bush bean varieties.
Speakers
MR

Md Rubel Reza

Kentucky State University
Co-authors
MC

Milon Chowdhury

Kentucky State University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Propagation
  • Subject Propagation
  • Poster # csv
  • Funding Source EVENS-ALLEN

1:00pm CDT

PROP - Physiology and Susceptibility of Avocado Trees to Laurel Wilt on Unreleased Experimental Phytophthora-Resistant Rootstocks
Thursday July 31, 2025 1:00pm - 1:45pm CDT
The University of California Riverside (UCR) rootstock breeding program has selected five advanced avocado (Persea americana Mill.) rootstocks for their resistance to Phytophthora Root Rot (PRR), a major disease affecting avocados worldwide. Another significant threat to avocado trees is laurel wilt (LW), caused by the fungus Harringtonia lauricola. To date, laurel wilt has been reported in 12 states, but has only infected avocado trees in Florida, where it has killed approximately 290,000 trees worth an estimated $95 million. It is anticipated that laurel wilt will eventually spread to California, the leading avocado production region in the U.S., as well as to Mexico and South America, where the largest world-wide avocado production occurs. For the UCR experimental PRR-resistant rootstocks to be successful in the long term after their commercial release, they should positively influence the LW resistance of ‘Hass’ and other scions. In this study, we tested the LW susceptibility and physiological responses of ‘Donnie,’ ‘Hass,’ ‘Gem,’ and ‘Reed’ scions grafted onto UCR advanced clonal rootstocks PP35, PP40, PP42, PP45, and PP80 under climate-controlled greenhouse conditions. All scions were also grafted and tested on ‘Waldin’ seedling rootstock, which is the primary avocado rootstock used in South Florida. Trees of each scion/rootstock combination were either inoculated with H. lauricola or with deionized water as a control. There were six single-plant replicates per treatment for each scion/rootstock combination, arranged in a completely randomized design. Visual disease symptoms were evaluated on a scale of 1 to10 (1= no symptoms, 10 = severe symptoms) every 3 to 4 days from the time of inoculation until the trees were harvested. Physiological variables, including leaf gas exchange (net CO2 assimilation, stomatal conductance, and transpiration), were also measured. At the end of the experiment, all plants were harvested, and the presence or absence of H. lauricola was confirmed by plating stem sections on CSMA (cycloheximide-streptomycin malt agar) medium. Although all scion/rootstock combinations were susceptible to LW, there were differences in susceptibility among combinations. The combinations ‘Hass’/PP45, ‘Gem’/PP40, ‘Gem’/PP80, ‘Hass’/Waldin’, and ‘Gem’/’Waldin’ exhibited the greatest tolerance to LW, as indicated by fewer external symptoms and less reduction in leaf gas exchange variables compared to the other combinations tested. Therefore, these more LW-tolerant scion/rootstock combinations have potential for planting in areas where both PRR and LW diseases occur. The most tolerant combinations identified in this study continue to be evaluated under field conditions for horticultural characteristics and yields in Florida.
Speakers
BS

Bruce Schaffer

Professor, University of Florida
Co-authors
EA

Edwin A. Gutierrez-Rodriguez

University of Florida
NA
JH

Jonathan H Crane

University of Florida, TREC
PM

Patricia M. Manosalva

University of California Riverside
NA
RG

Romina Gazis

University of Florida
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Propagation
  • Subject Propagation
  • Poster # csv
  • Funding Source USDA-SCRI Grant #:2024-51181-43302

1:00pm CDT

PROP - Propagation Strategies on Four Grapevine Varieties
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Improving stem cutting propagation of grapevine species holds immense importance with the growing interest in grapevine cultivation in the region. Propagating these plants from stem cuttings can be a challenging endeavor. As a result, this research initiative seeks to address this issue by exploring methods to enhance the success rate of stem cutting propagation. By developing more efficient and reliable techniques, nurseries can increase their ability to supply grapevines to local growers, which not only expands the availability of grapevines in the market but also plays a pivotal role in bolstering the wine industry in Mississippi. By facilitating the growth of this industry, improved grapevine propagation methods contribute to economic development, agricultural diversification, and ultimately benefiting the local economy and wine enthusiasts. Hardwood and semi-hardwood cuttings of four bunch grape varieties: ‘Miss Blanc’, ‘MidSouth’, ‘Norton’ and ‘Mamont Noir’ were subjected to 10 hormone treatments of indole-3-butyric acid (IBA) alone and in combination with ascorbic acid. These treatments were applied as a basal quick dip. Sixty days after treatment, rooting percentage, growth index (new shoots), cutting quality (0-5, with 0 = dead and 5 = transplant-ready cutting), total root number, average root length (of three longest roots), and root quality (0-5, with 0=no roots and 5=healthy, vigorous root system) data were collected. Of the four varieties trialed, ‘Miss Blanc’ performed the best with the greatest rooting percentage of 95% as a hardwood cutting and ‘Mamont Noir’ performed the best as a semi-hardwood cutting with the greatest rooting percentage of 100%. ‘MidSouth’ and ‘Norton’ will both require further research to assess the best rooting option for these varieties.
Speakers
JR

Jenny Ryals

Mississippi State University
Co-authors
ES

Eric Stafne

Mississippi State University
NA
HW

Haley Williams

Mississippi State University
PK

Patricia Knight

Mississippi State University
NA
SL

Scott Langlois

Miss State University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Propagation
  • Subject Propagation
  • Poster # csv
  • Funding Source Small Fruit Consortium 2024-R-14 and Hatch project MIS-211150 under accession number 7003104.

1:00pm CDT

PROP - Tissue Culture Propagation of Tea in the United States
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Tea (Camellia sinensis) has the potential to be cultivated as a commercial crop in the United States, but growers need access to clean plant material. The wholesale value of tea in the United States was $13.6 billion in 2022, yet less than 100 acres of tea are currently grown here. Tissue culture can be a means to provide disease-free and genetically identical plants. Three experiments were carried out using explants, which are 2.5 cm stems with single, active nodes from the young shoots of 28-year-old ‘Minto Pacific’ plants. Experiment 1 tested chemo- and thermotherapy: 40 explants were placed in Murashige and Skoog (MS) medium supplemented with 2, 4-dioxo-hexahydro-1-3-5 triazine (2 mL/L), aspirin (1.4 mL/L), and ribavirin (1.5 mL/L). Explants were placed in a growth chamber at 25 ± 1 ℃ on a 16-h photoperiod for 4 to 5 weeks, then subcultured and placed in an incubator for an additional 4 to 5 weeks set at 32 to 35℃ on the high end/light period and 28 to 30℃ on the low end/dark period (alternating every 4 h). All explants were either contaminated or dead, potentially due to high temperatures, by the end of this 8 to 10 week cycle. Experiment 2 evaluated use of meristems, which were excised from axillary buds of the aforementioned young shoots of 28-year-old ‘Minto Pacific’ plants and placed on MS media supplemented with 6-benzylaminopurine (1 mL/L) and gibberellic acid (0.5 mL/L). Explants were placed in a growth chamber at 25 ± 1 ℃ on a 16-h photoperiod. Of the 10 meristems, there was no contamination, but only one meristem has survived long-term (5 months). With Experiment 3, explants (n=26) were soaked in 4% v/v Plant Preservative Mixture (PPM) for 2 h, then transferred to MS medium, before being placed in a growth chamber at 25 ± 1 ℃ on a 16-h photoperiod. Contamination and physiological disorders developed over time, leaving only one visually healthy explant after 5 months. The effect of PPM as a soak or a growth medium-additive needs to be further investigated through additional experiments.
Speakers
MS

McKenzie Shelton

Washington State University
Co-authors
CM

Carol Miles

Washington State University
CM

Chakradhar Mattupalli

Washington State University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Propagation
  • Subject Propagation
  • Poster # csv
  • Funding Source Western SARE, Northwest Agriculture Research Foundation

1:00pm CDT

TECH 2 - Detection and Quantification of Onion Seedlings Lying on the Ground Using UAV Imagery and YOLOv8 in the Vidalia Region
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Vidalia sweet onion (Allium cepa spp.), holds significant economic and cultural value in the Vidalia region, known for its sweet flavor and low pungency. The success of marketable bulb production is closely linked to the early crop stages, particularly seedling production and field-quality transplanting. Due to the limited availability of mechanical transplanters, transplanting is predominantly performed manually, resulting in variability in planting quality. A major issue is the presence of seedlings lying on the ground, which significantly reduces survival rates and leads to undersized bulbs. Assessing the number of such seedlings manually is labor-intensive and inefficient. Therefore, this study aimed to develop a rapid and accurate method to quantify the number of seedlings lying on the ground across the field using a unmanned aerial vehicle (UAV) imagery and the YOLOv8 deep learning model. On January 6, 2025, aerial RGB images were captured using a UAV over an experimental onion field located at the UGA Vidalia Onion and Vegetable Research Center in Lyons, GA. The seedlings were transplanted on December 15, 2024, under three treatment conditions: 100% properly transplanted plants, 100% lying on the ground, and 25% lying on the ground. The objective of this study was to detect and quantify the percentage of plants lying on the ground within each plot using computer vision techniques. The images were manually annotated and used to train a YOLOv8 object detection model. The dataset was split into 70% for training, 15% for validation, and 15% for testing. Model performance was evaluated using standard YOLOv8 accuracy metrics, including precision, recall, and F1-score. The YOLOv8 model demonstrated strong performance in detecting onion seedlings lying on the ground. It achieved moderate to high precision and recall, as well as a high F1-score on the test dataset. When applied to the annotated aerial images, the model accurately quantified the percentage of fallen seedlings within each treatment. In the 100% properly transplanted treatment, a small number of seedlings were falsely identified as lying on the ground. These results confirm the model's ability to reliably distinguish between transplant quality levels using UAV imagery. This approach offers a scalable solution for monitoring transplant quality and identifying problematic planting zones.
Speakers
avatar for Regimar dos Santos

Regimar dos Santos

University of Georgia
Bachelor's degree in agronomic engineering from the Federal University of Mato Grosso do Sul, Brazil at 2021. Master's degree in plant production with an emphasis on computational intelligence in genetic improvement at 2022, with a doctorate in progress at the state university of... Read More →
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TECH 2 - Developing an AI-Driven Tool for Enhanced Daylily Flower Image Processing
Thursday July 31, 2025 1:00pm - 1:45pm CDT
This study developed an AI-powered image preprocessing tool to analyze daylily flower traits, providing a user-friendly and feature-rich solution for accurate image segmentation- a process that separates the flower from the background in photographs. The tool's design combines a pre-trained TRACER convolutional neural network (CNN) with an EfficientNet backbone to ensure robust and precise segmentation. It features a user-friendly graphical interface built with PyQt that facilitates seamless image uploads, real-time visualization of segmentation results, and customization options. Furthermore, a built-in manual refinement pencil function allows for precise corrections to automated masks, ensuring accuracy when needed. It supports multiple output formats, including masks and annotated images, enabling easy integration into subsequent analyses. A dataset comprising 1,450 daylily color images was employed for training and evaluating the tool. A comparative analysis of four deep learning models (TRACER, BASNet, U2 Net, and DeepLabV3), using metrics such as Accuracy, Precision, Recall, Dice Score, Jaccard Index, and XOR Error, highlighted the superior performance of the TRACER model in terms of accuracy and reliability. The findings show that the tool successfully separates flowers from intricate backgrounds, effectively addressing issues such as petal overlap, various phenotypes, and environmental variations. This automation tool has the potential to replace the labor-intensive and error-prone manual segmentation process, which has posed a significant limitation in AI applications for daylily research, including accurate trait prediction and classification.
Speakers
RG

Ramana Gosukonda

Fort Valley State University
Co-authors
CD

Chunhua Dong

Fort Valley State University
NA
PK

Priyanka Kumar

Fort Valley State University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Technology Applications in Horticulture 2
  • Subject Technology Applications in Horticulture
  • Poster # csv
  • Funding Source This work is supported by the 1890 Capacity Building Program, project award no. 2024-38821-42107, from the U.S. Department of Agriculture’s National Institute of Food and Agriculture.

1:00pm CDT

TECH 2 - Implementation and Evaluation of a Low-Cost Vision-Based Targated Weed Spray Technology
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Broadcast herbicide applications across fallow fields with sparse weed populations can lead to substantial chemical waste, increased costs, and environmental impacts. To address this issue, we implemented and evaluated a low-cost, vision-based targeted herbicide spraying system leveraging the Open Weed Locator (OWL) methodology. OWL utilizes simple RGB cameras and image-processing algorithms to detect green vegetation (weeds) against soil backgrounds in real-time, triggering targeted herbicide application only where weeds are detected. The primary objective of this study is to assess the practical effectiveness of this technology in field conditions, focusing on use-cases relevant to fallow weed management scenarios. Field trials are planned to quantify the system’s performance by evaluating weed detection accuracy through metrics such as recall and precision. Additionally, we will measure the percentage of herbicide chemical savings compared to conventional broadcast methods. Preliminary analyses suggest that this targeted approach significantly reduces chemical input. Detailed evaluations from forthcoming field trials will provide essential data to guide future improvements and support broader adoption of affordable, precision weed management tools.
Speakers
MS

Manpreet Singh

University of California Agriculture and Natural Resources
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TECH 2 - Integrating Multispectral Imaging and Low-Field Magnetic Resonance Imaging for Comprehensive Phenotyping of Horticultura
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Accurate plant phenotyping is essential for crop improvement but remains a major challenge, especially when tracking both above- and below-ground traits over time. Traditionally, these traits are measured manually and destructively, limiting data quantity and quality. At the Texas A
Speakers Co-authors
FN

Fahimeh Nia

Texas A
NA
LR

Lorenzo Rossi

Texas A
Dr. Rossi’s research program focuses on understanding the responses of horticultural crops to environmental stresses, with the goal of developing environmentally sound and effective management strategies. He is a horticulturist with expertise in plant stress physiology, plant biology... Read More →
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TECH 2 - Integrating Remote Sensing and AI for Rapid Nitrogen Assessment in Cannabis sativa L.
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Hemp (Cannabis sativa L.) is a remarkably versatile crop with extensive applications in food, fiber, and medicine, offering environmentally sustainable and highly productive raw materials across various industries. Historically cultivated primarily for fiber, modern dual-purpose hemp varieties now present enhanced economic opportunities by enabling the simultaneous harvesting of seeds for grain and stems for fiber. Nitrogen (N) fertilization significantly influences key growth parameters, including plant height, stem diameter, biomass accumulation, and seed yield. However, conventional nitrogen assessment methods are invasive and labor-intensive. To address these challenges, multispectral drone imaging has emerged as a non-destructive alternative, leveraging correlations between nitrogen levels and leaf chlorophyll content to enable rapid monitoring of critical physiological indicators such as assimilation rates, stomatal conductance, and transpiration rates. In 2024, an experiment was conducted at North Carolina A0.70), chlorophyll content and BNDVI (R² = 0.55), stomatal conductance and NDVI (R² = 0.82), and transpiration rate and MCARI (R² = 0.56). In contrast, negative correlations were observed with SIPI2 (R² = 0.69), TGI (R² = 0.39), and additional SIPI2 indices (R² = 0.54 and R² = 0.39, respectively). This study highlights the potential of integrating drone-based remote sensing and machine learning for efficient, non-destructive monitoring in hemp cultivation. By advancing precision agriculture practices, these technologies offer promising pathways to enhance productivity, optimize nitrogen management, and promote sustainability in hemp cultivation.
Speakers
HS

Harmandeep Sharma

Research Assistant Professor, North Carolina Agricultural and Technical State University
Co-authors
AB

Arnab Bhowmik

North Carolina A
GG

Gregory Goins

North Carolina A
NA
HS

Harjot Sidhu

North Carolina A
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Technology Applications in Horticulture 2
  • Subject Technology Applications in Horticulture
  • Poster # csv
  • Funding Source This work is supported by the Evans-Allen project award no NC.X-355-5-23-130-1 from the U.S. Department of Agriculture’s National Institute of Food and Agriculture.

1:00pm CDT

TECH 2 - Modeling Leaf Nitrogen in Organic Celery using VIS-SWIR Reflectance Spectra and Partial Least Squares Regression
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Nitrogen (N) management is a major challenge in organic vegetable production, aiming to supply sufficient N for optimal yield and quality while minimizing N losses. These challenges are exacerbated by uncertain mineralization patterns of different organic fertilizer products and season-specific impacts on soil N availability. Monitoring plant tissue N dynamics throughout the production season can provide meaningful information regarding fertility management, but sampling plant tissue can be labor-intensive and costly, and lab analysis may be time-consuming. Considering that N from organic nutrient sources is generally not immediately available for crop uptake, the need for reliable tools to rapidly monitor plant N status is paramount in improving N use efficiency, particularly in organic systems. In this study, a hyperspectral imaging approach was explored. Celery samples were collected from two research trials repeated in two production seasons at midseason (approximately 70 days after transplanting; DAT) and final harvests (approximately 110 DAT). One experiment focused on integrated nutrient management practices, comparing celery grown following a sunn hemp cover crop to a weedy fallow (whole plots), and evaluating impacts of composts (subplots) including an unamended control, yard waste compost, vermicompost, and a mixed compost treatment. The other experiment compared ratios of preplant N:in-season N application from 0-100% preplant N (whole plots) under two organic preplant fertilizers (subplots) contrasting in composition and anticipated N mineralization. At each harvest, six plants from each experimental unit were weighed and allocated into representative portions for crop quality analyses on fresh and dry bases. A spectroradiometer with a leaf clip reflectance probe was used to collect leaf reflectance spectra (350-2500 nm) from approximately eight leaves from each experimental unit. Spectra were normalized to a mean of zero and a standard deviation of one across wavelengths. To ensure balanced representation in training and validation, the data were stratified by harvest timing, trial, and year, and randomly split following 80% calibration and 20% external validation distribution. The final model used 19 latent components, explained 76.4% of the variation within external validation data, and had a root mean square error of 0.36. The model can be categorized as providing “approximate quantitative predictions,” and total N content from dried aboveground biomass in the original dataset spans from 1.0 - 4.3 g N/100 g DW. Building robust models using hyperspectral data to predict crop N status under diverse production practices and environmental conditions is an area deserving of continued research in organic vegetable production.
Speakers
XZ

Xin Zhao

University of Florida
Co-authors
AS

Aditya Singh

University of Florida
NA
MC

Moses Chilenje

University of Florida
NA
SL

Stephen Lantin

University of Florida
NA
ZR

Zachary Ray

University of Florida
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Technology Applications in Horticulture 2
  • Subject Technology Applications in Horticulture
  • Poster # csv
  • Funding Source This work is supported by the Organic Agriculture Research and Extension Initiative program, project award no. 2019-51300-30243, from the U.S. Department of Agriculture’s National Institute of Food and Agriculture.

1:00pm CDT

TECH 2 - Monitoring Hurricane Effects in Pecan Fields: An Object Detection Framework to Detect Fallen Trees
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Disaster events such as hurricanes strongly affect pecan crop production due to fallen trees, which generate a significant impact on production areas. An appropriate method to monitor these effects is by counting the fallen trees. This monitoring is commonly performed by a field team. Although accurate, this approach is time-consuming, costly, and non-scalable. Consequently, an image-based approach emerges as a timely opportunity. For instance, aerial images captured by unmanned aerial vehicles (UAVs) present high spatial resolution and coverage, contributing to tree identification. However, relying solely on images remains a laborious and time-consuming task. Consequently, the development of object detection models to automatically identify and count fallen trees emerges as an unprecedented and dynamic method. Therefore, we aimed to integrate UAV image technology and the YOLOv12 detection model for detecting fallen trees in pecan fields. On September 27th, 2024, the hurricane Helene crossed the State of Georgia, USA. On October 7th, 2024, we captured RGB images using a multirotor UAV. We captured high-resolution (~3 cm/px) in eight pecan fields. The images were processed to generate one orthomosaic for each field. Consequently, we counted the fallen trees using assisted image processing for ground truth data. Together, the pecan fields presented more than 200 fallen trees. Subsequently, we trained the YOLOv12 model to automatically identify the fallen trees. Initially, we manually labeled all the fallen trees as “Fallen”. Afterward, we split our dataset into training (70%), validation (15%), and testing (15%). To enhance model performance and generalizability, we implemented data augmentation for the training dataset. As a result, the model achieved identification metrics of fallen trees higher than 70%. Certainly, this approach presents a great opportunity to measure the effects caused by hurricanes, giving farmers the ability to make faster and better decisions regarding their fields. Ultimately, these findings support precision agriculture practices and introduce pecan crops into this context, further strengthening the introduction of technologies into the world of specialty crops.
Speakers
avatar for Marcelo Barbosa

Marcelo Barbosa

University of Georgia
Co-authors
LW

Lenny Wells

University of Georgia
NA
LO

Luan Oliveira

University of Georgia
LS

Lucas Sales

University of Georgia
Agronomy Engineer graduated from the Federal University of Paraíba. With experience in the management and cultivation of Ornamental Plants, through a year of experience working in Greenhouses in the state of New Hampshire, USA. Experienced in the management and cultivation of vegetables... Read More →
RD

Regimar dos Santos

University of Georgia
Bachelor's degree in agronomic engineering from the Federal University of Mato Grosso do Sul, Brazil at 2021. Master's degree in plant production with an emphasis on computational intelligence in genetic improvement at 2022, with a doctorate in progress at the state university of... Read More →
VM

Victor Martins

University of Georgia
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 2 - Assessing Saltwater Tolerance of Mustard Greens (Brassica juncea) Genotypes using Hydroponics
Thursday July 31, 2025 1:00pm - 1:45pm CDT
With freshwater supplies dwindling and sea levels rising, irrigation water for crops can become salinized. Therefore, salt-tolerant genotypes of food crops are needed. Here, we tested genotypes of Brassica juncea (brown mustard), a widely grown and consumed leafy green that has mineral and nutrient rich leaves and is also an important oil-seed crop, for their salt tolerance. Experiments were conducted to evaluate the performance of three mustard genotypes (var. ‘Florida Broadleaf’, ‘Carolina Broadleaf’, and ‘Southern Giant Curl’) grown in nutrient film-technique (NFT) hydroponic systems under varying salinity treatments. Five synthetic seawater dilutions (15, 22.5, 30, 37.5, and 45% Instant Ocean at approximately 10, 13.5, 17, 21, 24.5 dS/m conductivity, respectively) with added fertilizer (Masterblend 20-20-20 at approx. 1.5 dS/m) were compared to a control (fertilizer only at approx. 1.5 dS/m) to identify the optimal salinity range for vegetative production over 6 weeks after seeding. Two different production systems were used to evaluate the genotypes: production-scale NFT systems in a greenhouse in Citra, FL, and lab-scale tabletop NFT systems in an environmentally controlled room. In the greenhouse setting, plants were destructively harvested at 7 day intervals to gather growth over time, while in the lab setting, plants were harvested once at the end of the experiment to calculate the biomass and growth along with water and nutrient use efficiencies per genotype. Preliminary findings suggest that yields and water use efficiencies were improved at a 15% seawater dilution (approx. 10 dS/m) and showed a drop in biomass at higher seawater dilutions. The genotype ‘Florida Broadleaf’ demonstrated higher fresh biomass than either of the other genotypes with ‘Southern Giant Curl’ showing the most sensitivity to salt treatments in both the greenhouse and desktop systems. Data collected in these experiments will be used to construct a dynamic crop growth model for mustards that incorporates salinity to predict crop biomass. Crop models such as this could help inform plant breeders studying salt-tolerance and provide insights into yields and crop growth rates to agricultural producers farming in salt-afflicted soils or for use in saltwater hydroponics.
Speakers
JP

Jean Pompeo

University of Florida
Co-authors
DH

Daniel Hofstetter

University of Florida
NA
KR

Karthikeyan Raghupathy

Clemson University
NA
MC

Melanie Correll

University of Florida
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 2
  • Subject Vegetable Crops Management
  • Poster # csv
  • Funding Source USDA-AFRI-SAS-2023-69012-39038; USDA-HATCH-ABE-006356; USDA-NIFA-McIntire-Stennis Capacity Grant

1:00pm CDT

VCM 2 - Divergent Leaf Physiology in Kale Cultivars Under Organic and Conventional Farming Systems
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Kale (Brassica oleracea var. acephala), a member of the cruciferous (Brassicaceae) family, has gained significant popularity as a nutritionally dense leafy vegetable in recent years, attributed to its rich nutraceutical properties, including phytochemicals, antioxidants, and antiproliferative compounds. These properties play a critical role in addressing global nutritional deficiencies through cost-effective dietary sources, leading to kale’s designation as a superfood by the research community. Its popularity has steadily increased, particularly in the United States. To meet the growing demand for kale, optimizing production systems is essential. In this study, we evaluated leaf physiology, yield parameters, and nutrient content across kale cultivars grown under both organic and conventional farming systems. We observed substantial variation in leaf photosynthesis and other physiological traits, reflecting the different responses of cultivars to cultivation methods. Additionally, significant differences in mineral content and total soluble proteins were noted, with organic farming practices notably enhancing soluble proteins, leaf nitrogen content, nitrate nitrogen (NO3-N), and several key minerals compared to conventional practices. These findings suggest that organic farming practices may enhance the nutraceutical quality of kale, potentially offering superior nutritional benefits compared to conventional methods systems.
Speakers Co-authors
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 2

1:00pm CDT

VCM 2 - Effect of biochar and vermicompost to mitigate salinity stress in high-tunnel grown tomato
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Saline water irrigation, higher summer temperatures, limited soil leaching and agricultural intensification with heavy reliance on chemical fertilizers are the main causes for high salt accumulations in high-tunnel soils. These salinity issues have led to soil degradation as well as decline in crop production. In order to improve soil health and bolster the crop yield, use of organic amendments like compost, crop residues and animal manure has been widely practiced. Studies have shown that biochar and vermicompost can improve soil homeostasis by adsorbing excess sodium and chloride ions and holding excess water in root zone. Furthermore, researchers have found that these soil amendments complement each other when used in combination, leading to high crop yield and enriched plant nutrient contents. However, research gaps exists in using emerging soil amendments like biochar, and vermicompost. Hence, the objective of our current study was to assess the growth and yield attributes of tomato under salinity stress and evaluating the role of these soil amendments alone and in combination to mitigate salt stress. Two potted experimental trials were conducted in late spring and early fall in high tunnel at Oklahoma State University. The treatments were arranged in a complete randomized design. Treatments included three levels of salinity (1.5, 4, 8 mS/cm) and different treatment combinations (0, 5, 10% v/v) of biochar and vermicompost in soilless media. Data collection was done on different yield parameters, phytochemicals, stress indicators along with soil media tests. Results show that soil amendments treatments helped reduce salinity stress to some extent and aid in overall crop performance. To sum up, our salinity stress study provides insights on minimizing the salt toxicity by appropriate use of emerging soil additives, providing practices for producers to improve crop production in high-tunnels.
Speakers
PP

Pradeep Paudel

Oklahoma State University
An MS student at Oklahoma State University, interested in the use of organic amendments for crop production and soil health improvement.
Co-authors
BC

Bee Chim

Oklahoma State University
NA
NM

Niels Maness

Oklahoma State University
NA
SK

Shivani Kathi

Oklahoma State University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 2 - Effect of Selenium Fertilization on Carotenoid and Nutritional Accumulation in Watercress with Deep Water Hydroponics
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Nutrient biofortification in leafy vegetables can enhance dietary health benefits, improve crop nutritional quality, and promote sustainable agricultural practices. Deep water culture hydroponic production along with micronutrient exploration can lead to solutions to optimize nutrient uptake. Selenium (Se), an essential micronutrient, has been shown to influence plant metabolism, particularly the synthesis of bioactive compounds such as carotenoids and glucosinolates. However, its role in modulating these phytochemicals in hydroponically grown Nasturtium officinale (watercress) remains underexplored in controlled environment agriculture systems. This study investigates the effects of selenium fertilization at varying concentrations (0, 1.0, 2.0, and 4.0 mg Se·L⁻¹) on carotenoid and glucosinolate accumulation in water cress varieties cultivated in deep water hydroponics. The controlled environment environment system provides an ideal environment for plant growth, allowing precise manipulation of nutrient levels, pH, temperature, and light intensity. The selenium treatments are based on a randomized complete block design to ensure statistical accuracy. Various growth parameters, biomass accumulation, and biochemical analyses of carotenoid and glucosinolate levels were measured to determine the interactions between selenium uptake and metabolite biosynthesis. Carotenoid content was quantified using high-performance liquid chromatography (HPLC). Glucosinolate concentrations were determined through chromatographic and spectrophotometric methods to measure bioactive compound content. Research suggests that selenium supplementation may modulate plant physiological responses and enhance carotenoid and glucosinolate synthesis. It is essential to study ways to optimize hydroponic deep water culture production systems to improve the nutritional and functional quality of leafy greens like watercress. These findings can provide information to guide controlled environment and sustainable agricultural practices. Results from this research may also advance nutrient-fortification strategies, enhance functional food development, and address micronutrient deficiencies, thereby supporting both horticultural innovation and public health. Keywords: Selenium fertilization, Beta-carotene, Deep Water Hydroponic, Watercress, Agricultural sustainability, Controlled Environment Agriculture
Speakers
avatar for Cora Teets

Cora Teets

Research Associate, Kentucky State University
Hi! I am Cora Teets, I am a resercher in the Center Nutritrition and Cancer for Resrach at Kentcuky State Universoty. I also act as an adjunct professor for Basic Nutrition at the University. I am passionate about mentoring students and establishing collaborations to make the most... Read More →
Co-authors
AA

Adekunle Adeyeye

Kentucky State University, Frankfort, KY
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 2
  • Subject Vegetable Crops Management
  • Poster # csv
  • Funding Source USDA ARS: 210238-20047-206002-3100
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

1:00pm CDT

VCM 2 - Effects of Biostimulant Application on Plant Growth, Fruit Yield, and Quality of Six Sweet Peppers Cultivars
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Sweet peppers are a warm-season vegetable prized for their vibrant colors, rich flavor, and high nutritional value. Biostimulants are a new class of agricultural products consisting of microorganisms or bioactive substances, used as a sustainable approach to enhance plant growth and productivity. This experiment aimed to evaluate the effects of biostimulant applications on plant growth, yield, and quality of sweet pepper cultivars grown in a container production system. Three biostimulants were tested including a seaweed extract (Kelpak Maxx, 1% solution), a plant growth-promoting rhizobacteria (Continuum V2, at a rate of 2 ml per gallon), and stabilized monosilicic acid (Dune, at a rate of 2 ml per gallon). Six sweet pepper cultivars were selected for the study: Cornito Giallo, Escamillo, Green Machine, Carmen, SVPB8500, and Cornito Rosso, all purchased from Johnny’s Selected Seeds. Biostimulant treatments were applied biweekly through manual fertigation with 120 ml of solution. Plants in the control group were fertigated with 120 ml of water. Plant vegetative growth including plant height, widths, and leaf SPAD were measured three times during the growing season. Once fruit ripening began, pepper fruits were harvested every two weeks from July to September. At each harvest, marketable and unmarketable yield and fruit number were recorded. Fruit quality variables including fruit length, diameters, and color were also measured. Results showed that the sweet pepper cultivars varied in vegetative growth, fruit yield, and quality. The two cultivars Green Machine and SVPB8500 had higher leaf SPAD of 64.2 and 63.9, respectively, than Carmen, Escamillo, Cornito Giallo, or Cornito Ross. The cultivars Carmen, Cornito Giallo, Cornito Rosso, and Escamillo had similar plant heights ranging from 36.4 cm to 38.4 cm, higher than Green Machine or SVPB8500. Biostimulant treatment did not affect leaf SPAD or plant height early in the season.
Speakers
AP

Abby Pennington

Mississippi State University
Co-authors
AA

Ali Alsughayyir

Mississippi State University
NA
GB

Guihong Bi

Mississippi State University
JA

Jacob Arthur

Mississippi State University
TL

Tongyin Li

Mississippi State University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 2
  • Subject Vegetable Crops Management
  • Poster # csv
  • Funding Source United States Department of Agriculture (USDA) Mississippi Department of Agriculture and Commerce Specialty Crop Block Grant Program; United States Department of Agriculture (USDA) National Institute of Food and Agriculture Hatch Project MIS-149220.

1:00pm CDT

VCM 2 - Influence of Silicon and Brassinosteroids Application on Yield and Nutrient Composition of Arugula
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Rising temperatures, erratic precipitation, and increased periods of drought present significant challenges to vegetable crop production by disrupting growth reducing yield and crop quality. To address these challenges, biostimulants have been used in crop production for their ability to enhance plant growth, improve nutrient uptake, and increase resilience against environmental stresses. However, there exists a research gap in the mode of applications and rates of different biostimulants in various vegetable crops grown in open-field conditions. Hence, the current study evaluated the effects of two distinct biostimulants (silicon and brassinosteroids) on arugula (Eruca sativa) production. Although silicon is not universally recognized as an essential nutrient for plant growth, it is considered beneficial and has been proven to exhibit biostimulant properties. Contrarily, brassinosteroids are a class of plant hormones crucial in regulating growth, development, and stress responses in crops. These biostimulants were applied independently through the soil and foliar application at two different rates to assess their effects on the growth, yield, and nutritional quality of arugula. Treatments were arranged in a randomized complete block design with three blocks in open-field conditions. Data was collected on various parameters, including the number of leaves per week, fresh and dry weight at harvest, and mineral and phytochemical content such as chlorophylls, carotenoids, sugars, phenols, and flavonoids content. Results show that silicon applied plants have improved yield. By assessing biostimulants impact on the yield and nutrient composition of arugula, the current research concludes the potential of biostimulants as a sustainable approach to improve growth of arugula under challenging environmental conditions
Speakers
NR

Nandini Reddy Muraka

oklahoma state university
Co-authors
NM

Niels Maness

Oklahoma state university
NA
SP

Steve Phillips

Oklahoma state university
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 2 - Leaf Anatomical and Physiological Variability of Tomato Genotypes with Modified Polyamine Metabolism and its Potential Role to Stress Resilience
Thursday July 31, 2025 1:00pm - 1:45pm CDT
The anticipated impacts of climate extremes on food production have led research into novel paths aimed at optimizing cultivation techniques and developing genotypes resilient to abiotic stresses. Research over the last 20 years led to a considerable amount of evidence that the regulation of polyamine metabolism (and in particular of thermospermine) through the overexpression or gene mutation of polyamine oxidases (PAOs) genes, could enhance plant resilience. However, there are still limited data on how the differential expression of PAOs genes affects physiological and morpho-anatomical traits that contribute to plant resilience. Therefore, in this study we explored the morpho-anatomical and functional-physiological variability of leaves of 8 tomato genotypes, one reference (cv. Moneymaker), and 7 with modified thermospermine metabolism, comprising 3 overexpressing genotypes and 4 loss-of-function mutants for two thermospermine-specific PAO genes, SlPAO3 and SlPAO4. The plants were cultivated under greenhouse conditions following standard commercial practices, and a total of 17 morpho-anatomical and functional traits were assessed for each genotype. Based on plasticity indices (IP), notable phenotypic variability among genotypes was observed, particularly in leaf morpho-anatomical traits, pigment concentrations, performance index (PItotal), plant height, and total leaf area. In contrast, the lowest IP values were recorded for traits related to gas exchange and Fv/Fm. Principal component analysis (PCA) identified two main axes accounting for 78% of the total variation, and a clear grouping of genotypes emerged according to the type of PAOs gene expression modification (overexpression or gene mutation). These findings reveal substantial differences in leaf structural traits among genotypes, depending on PAOs expression levels, aligning with previous studies highlighting the role of polyamines in organogenesis. Considering the critical importance of leaf structure–function interactions in determining both productivity and stress resistance, further investigation into the role of PAOs as a potential tool for enhancing plant resilience to abiotic stresses is warranted.
Speakers
IK

Ioannis Karapanos

University of Florida / North Florida Research and Education Center
Co-authors
AK

Andriana Karachaliou

Crop Science Athens Attica Greece, Agricultural University of Athens
DN

Dimosthenis Nikolopoulos

Crop Science Athens Attica Greece, Agricultural University of Athens
EK

Evangelos Kyrkoulis

Crop Science Athens Attica Greece, Agricultural University of Athens
GL

Georgios Liakopoulos

Crop Science Athens Attica Greece, Agricultural University of Athens
PB

Panagiota Bresta

Crop Science Athens Attica Greece, Agricultural University of Athens
PT

Paraskevi Tavladoraki

Department of Science, University Roma Tre
SC

Styliani Chorianopoulou

Crop Science Athens Attica Greece, Agricultural University of Athens
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VCM 2 - Leveraging the potential of Methylobacterium symbioticum to boost spinach productivity
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Nitrogen is a vital element for plant growth, playing a crucial role in photosynthesis and protein synthesis. The bio-stimulant "Utrisha N," which contains the bacteria Methylobacterium symbioticum, has the potential to enhance nitrogen availability by fixing atmospheric nitrogen, thereby promoting sustainable agricultural practices. While it has been evaluated in other row crops, its application in vegetable crops remains untested. This study examined the effects of the foliar application of "Utrisha N" on yield, nitrogen metabolism, and phytochemical profiles under various nitrogen conditions. The results indicated that the foliar application of "Utrisha N" during the developmental phase of spinach significantly increased both fresh and dry biomass and chlorophyll content. No significant changes in nitrogen mineral metabolism were observed regardless of nitrogen availability. We assessed nitrogen assimilation by calculating the percentage accumulation of amino acids in the vegetative tissues. The differential expression of genes related to nitrogen metabolism influenced by Methylobacterium symbioticum is currently being analyzed. This study's results will help clarify the potential benefits of Methylobacterium in improving nitrogen metabolism and boosting spinach productivity.
Speakers
avatar for Sandeep Sran

Sandeep Sran

Graduate Research Assistant, Texas A & M University, College Station
Co-authors
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 2

1:00pm CDT

VCM 2 - Physiological and Morphological Screening of Tomato Cultivars for Thermotolerance
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Temperature constraints on tomato production challenge local production in warm climates. In Oklahoma, there is a narrow optimum temperature window required for fruit set, which ultimately results in a decline during late July and August. Growers are motivated to select heat tolerant tomato genotypes for better results. Previous research shows that there is a range of physiological responses when considering heat tolerant and heat sensitive cultivars. To investigate further, a controlled environment trial will be designed to evaluate eight different tomato cultivars at two different temperature regimes, optimum (26°C / 20°C) and elevated (36°C/26°C). Morphological measurements will be collected daily and are to include days to first truss, days to first open flower, days to first fruit set, and flower-fruit set ratio. Non-destructive physiological measurements will be collected weekly, including net photosynthetic rate, stomatal conductance, leaf temperature, and chlorophyll fluorescence. Electrolyte leakage will be measured once upon the completion of the trial. Differences in net photosynthetic rate and stomatal conductance were found to be significant between the two temperature regimes (p > 0.001) at 28 days after transplanting. Net photosynthetic rate averages were 4.358 µmol m-2 s-1 for the elevated temperature and 7.815 µmol m-2 s-1 for the optimum temperature. Stomatal conductance averages were 0.081 mol m-2 s-1and 0.150 mol m-2 s-1 for elevated and optimum regimes respectively. A Euclidean Distance will be calculated between the responses to the two temperatures upon completion, creating a heat tolerant index. The results will assist with the development of a screening tool for heat tolerant cultivars.
Speakers Co-authors
JQ

Justin Quetone Moss

Oklahoma State University
NA
SK

Shivani Kathi

Oklahoma State University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 2

1:00pm CDT

VCM 2 - Production of Alternative Microgreen Species as Affected by Five Types Growing Substrate
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Microgreens are young seedlings of various vegetable and herb species that are usually harvested 10 to 14 days after germination. They are a popular specialty crop because they are rich in mineral nutrients and health beneficial phytochemicals, grow quickly, need little space, and can be produced year-round in a protected environment. The choice of substrate is critical for successful microgreen production because it affects shoot growth, nutrient absorption, and total shoot yield. Compared with commonly used peat-based substrate, easy-to-use hydroponic mat products made from various fibers serve as sustainable alternative growing media. This study examines the effect of five substrates on the growth performance of four microgreen species including ‘Red Garnet’ amaranth (Amaranthus tricolour), ‘Red Gruner’ purslane (Portulaca oleracea), Rainbow Sprinkles Mix (with chard and beet, Beta vulgaris), and Shungiku (Glebionis carinata). The five substrates include bamboo mat, coco coir fiber mat, Ectone mat (made from recycled plastic bottle), jute mat, and a peat based soilless substrate. Results showed that fresh shoot weight was affected by the interaction between species and substrate type. Shungiku grown on peat and coco coir produced the highest fresh shoot weights of 74.7 and 70.2 g per growing tray, respectively, among all treatment combinations. Microgreens species varied in their preference of substrate for maximum shoot yield, with peat producing in higher fresh shoot weight than other substrate types in purslane and Rainbow Sprinkles Mix. Overall, peat and coco coir mat resulted in higher dry shoot weight than jute, bamboo, or Ectone mat across the four tested species. These results show the importance of choosing the right substrate for maximum yield and quality in microgreen production.
Speakers
AA

Ali Alsughayyir

Mississippi State University
NA
Co-authors
AP

Abby Pennington

Mississippi State University
GB

Guihong Bi

Mississippi State University
JA

Jacob Arthur

Mississippi State University
TL

Tongyin Li

Mississippi State University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Crops Management 2
  • Subject Vegetable Crops Management
  • Poster # csv
  • Funding Source United States Department of Agriculture (USDA) Mississippi Department of Agriculture and Commerce Specialty Crop Block Grant Program; United States Department of Agriculture (USDA) National Institute of Food and Agriculture Hatch Project MIS-149220.

1:00pm CDT

WCPM 1 - Controlling Weeds in Nursery Container Production by Alternative Pesticides: A Potential Research Area
Thursday July 31, 2025 1:00pm - 1:45pm CDT
The ornamental crop industry in the United States, is a billion-dollar industry. Weed management in nursery container production is a significant challenge, as weeds compete with ornamentals for essential resources, reducing plant quality, marketability, and overall profitability. Controlling weeds in containers is one of the highest production costs encountered by nursery growers, often exceeding $4,000 per acre. Previous research results have shown that weeds within a restricted area can reduce ornamental growth by 60%. Some of the most problematic weed species identified in the container production includes liverwort, hairy bittercress, oxalis, chick weed, etc. Liverwort is one of the major weed problems in greenhouses and nursery container production. Historically liverwort has been reported as a weed issue in cooler regions of the Northeast and Pacific Northwest regions of the United States. It thrives well in moist, low UV light condition, and in presence of high fertility and a cool temperature. Hence, nurseries and greenhouses are ideal places for their growth. Growers rely mostly on hand weeding to manage these problematic weeds, especially inside the greenhouse production system as there are limitations in applying herbicides within greenhouse conditions. Since hand weeding is very time consuming, laborious, and expensive, there is an immediate need for research to improve chemical weed control practices through alternative pesticides that can be applied to container production and improve growers’ productivity and profitability. New plants are always being added to nursery production so phytotoxicity studies are also required. Some previous research has focused on evaluating synthetic versus organic herbicides for weed control inside greenhouse conditions. However, there is almost no information available on how alternative pesticides such as fungicide or miticides can be used for weed control in container production and whether they will be safe on ornamentals. This significant knowledge gap has led to the development of this master’s research proposal. Hence the objectives of this research proposal are: To evaluate the preemergence efficacies of fungicide and miticide as alternative pesticides on liverwort control; To assess newer herbicide formulations and rates for controlling weeds and phytotoxicity on container-grown woody shrubs; To disseminate the research results to the growers and scientific community. Preliminary results have shown that miticide Tetracurb Max and fungicide Zerotol can provide suppression of liverwort in containers without causing any significant injuries to the woody ornamental shrubs.
Speakers
SG

Sushil Grewal

Michigan State University
Co-authors
DS

Debalina Saha

Michigan State University
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

WCPM 1 - Germination Moisture and Temperature Requirements of Italian Ryegrass
Thursday July 31, 2025 1:00pm - 1:45pm CDT
The changing climate is impacting weed ecology and growth patterns in agricultural production systems. General understandings of temperature and moisture requirements have been documented for different weed species, however, there is evidence that production practices and environmental factors affect weed species response to temperature and moisture. Laboratory studies were initiated in 2023 at the University of Idaho Research and Extension Center to determine the germination temperature and moisture requirements of Italian ryegrass (Lolium multiflorum) and spring wheat (Triticum aestivum) collected in Idaho and Washington. For the germination temperature requirement experiments, weed populations and wheat cultivars were grown on a thermogradient table with 10 varying temperatures between 4 C and 35 C. In the germination moisture requirement experiments, polyethylene glycol (PEG 8000) obtain 10 different osmotic potentials (0 to -2 MPa) at temperatures >12 C, there were no differences in germination speed and maximum germination between the Italian ryegrass and spring wheat. However, Italian ryegrass had faster germination speed and greater maximum germination at temperatures
Speakers
CM

Chandra Montgomery

University of Idaho
Co-authors
AA

Albert Adjesiwor

University of Idaho
JC

Joan Campbell

University of Idaho
NA
TR

Traci Rauch

University of Idaho
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Weed Control and Pest Management 2

1:00pm CDT

WCPM 1 - Identifying Lactuca spp. Germplasm Resistant to Western Flower Thrips
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Recently, Impatiens necrotic spot virus (INSV), transmitted by the western flower thrips (Frankliniella occidentalis), has emerged as a major limiting-factor in lettuce production, causing up to 100% yield losses in California. Florida, the third-largest lettuce-producing state in the US, following California and Arizona, cultivates approximately 11,000 acres of lettuce annually. While cases of INSV have not been reported in Florida lettuce, F. occidentalis is a common pest in many crops in Florida including lettuce, the virus poses a significant threat to lettuce crops if introduced. Since INSV is exclusively transmitted by thrips, effective thrips management is crucial to mitigate its impact. Currently, management of F. occidentalis mainly relies on pesticides, however, over-reliance on pesticides may lead to insecticide resistance and further raising environmental and health risks. An integrated pest management approach, incorporating thrips-resistant lettuce cultivars as a first line of defense, could provide a sustainable solution to mitigate the possible negative effects of INSV. The aim of this study was to identify lettuce germplasm with resistance to F. occidentalis. In no-choice experiments, 39 lettuce germplasms including commercial cultivars were evaluated for the resistance to F. occidentalis. These germplasms were planted under thrips-free conditions and plants were placed individually to thrips-proof container and infested with 7 female thrips when they developed 5-6 true leaves. The thrips colony was reared on Okeechobee cultivar, a cultivar susceptible to other sap-feeding insects, which was also used as control for the experiments. Fourteen days post-infestation, the number of larvae and adult thrips per plant. Experiments were conducted in an insectary room maintained at 25 ± 1 °C with a photoperiod of 16-hour light and 8-hour dark. Five replicates (plants) were conducted for each lettuce germplasm. Several germplasms, including breeding lines 50100, 70096, and 70882, cultivars such as Bambino, La Brillante, Manatee, Emperor, Hacienda, and Valmaine, as well as plant introductions (PI) 204707 and 251246, exhibited significantly less larvae (Alpha level 0.05) than Okeechobee, suggesting resistance to F. occidentalis. On the other hand, breeding line 60183 and Gator had significantly higher number of larvae thrips than Okeechobee suggesting high susceptibility to F. occidentalis. These findings highlight promising candidates for further identification of genomic regions responsible for resistance against F. occidentalis and to develop resistant lettuce cultivars that could be a first-line of defense against INSV. Since INSV has not been reported in Florida, this underscores the importance of proactive resistance breeding efforts to prevent potential outbreaks.
Speakers
TB

Tennyson Bilinkhinyu Nkhoma

University of florida
Co-authors
DM

De-fen Mou

University of florida
NA
GS

German Sandoya Miranda

University of Florida EREC
JB

Julien Beuzelin

University of florida
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Weed Control and Pest Management 2
  • Subject Weed Control and Pest Management
  • Funding Source This project is partially supported by USDA NIFA Hatch project Accession Number 7005266 and USDA-AMS Specialty Crop Block Program/Florida Department of Agriculture and Consumer Services (FDACS) grant 23SCBPFL1181.​

1:00pm CDT

WCPM 1 - Investigation of laser-guided variable-rate spray technology to achieve thorough trunk applications
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Air-blast sprayers with intelligent spray technology can apply pesticides more efficiently to tree canopies than conventional sprayers. However, little research has examined their effectiveness for trunk applications to control pests such as flatheaded borer (FHB). FHB larvae chew directly into the trunk upon hatching, necessitating thorough coverage with contact insecticides. Our objective was to explore the potential of using intelligent variable-rate spray technology to achieve thorough coverage on tree trunks. A red maple (Acer rubrum) block was divided into three plot-row-types: single-row (SR), double-row (DR; South and North rows), and triple-row (TR; South, interior, and North rows). Water sensitive paper (WSP) wraps were used to assess spray coverage. Wraps were secured around trunks of ten trees per row at 15 and 40 cm above the ground. Plots were sprayed with water using a Jacto A400/850 air-blast sprayer in constant-rate (CR) and variable-rate (VR) modes using the Smart Apply intelligent spray control system. CR discharged 69% higher spray volume than the VR mode (P < 0.0001). SR had >99% coverage; coverage in SR was not affected by spray mode or wrap height (P ≥ 0.0592). For DR, coverage ranged from 98.4% to 99.9%, and spray mode did not affect coverage on upper wraps (P ≥ 0.0829) within a row, while CR (99.6%) had lower coverage than VR (99.9%; P = 0.0043) for lower wraps in the northern row. However, TR coverage varied with the spray mode and wrap height (P < 0.0001). Upper wraps in the southern and interior rows had higher coverage from CR than VR, 98.6% versus 89.9% (P = 0.0019) and 98.7% versus 78.8% (P = 0.0137), respectively. For lower wraps, both the southern and interior rows had higher coverage from CR than VR, 98.5% versus 93.4% (P = 0.0019) and 99.5% versus 94.0% (P = 0.0137), respectively. Coverage was very high, i.e., ≥98.0% for 18 of 24 treatment combinations and ≥89.9% for 23 of 24 treatment combinations, but did not achieve 100%. There was no advantage to CR for SR or DR. For all plot-row-types and spray modes, the spray rate was higher than desired. CR sprayed 480, 251, and 210 GPA in the SR, DR, and TR plots, respectively, while VR discharged 239, 156, and 140 GPA. Future research should examine the use of adjuvants to increase coverage and determine a coverage range that balances acceptable FHB control, labor costs, and spray volume.
Speakers
KM

Karl McKim

The University of Tennessee
Co-authors
AF

Amy Fulcher

University of Tennessee
CY

Cory Yurisic

University of Tennessee
NA
EE

Erica Eady

The University of Tennessee
NA
GP

Grace Pietsch

The University of Tennessee
NA
HZ

Heping Zhu

USDA-ARS
NA
SX

Sun Xiaocun

The University of Tennessee
NA
WC

Wesley C Wright

The University of Tennessee
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Weed Control and Pest Management 2
  • Subject Weed Control and Pest Management
  • Funding Source USDA SCRI Grant # 2020-51181-32199
  • Funding Option SCRI funded all or part of the research associated with this abstract

1:00pm CDT

WCPM 1 - Pest Silencing- RNA Interference as a Strategy for Pest Management
Thursday July 31, 2025 1:00pm - 1:45pm CDT
The current study seeks to improve our ability to utilize RNA interference in developing novel tactics for management of arthropod pests related to crop protection and vector mitigation. The use of this technology in the development of novel arthropod control strategies has been substantially limited relative to its potential for implementation, and inherent advantages over traditional means for controlling pests. Past efforts in applying RNA interference as a strategy for management of arthropod pests have been limited by substantial hurdles that this research seeks to address. While the potential for the utility of RNAi in pest management has been recognized in the past several decades, previous research has found that the practicability of the technology in an applied setting is stifled by a number of factors, including the molecular instability of RNA, host susceptibility, evolved resistance, efficacy, and off-target effects. Our interest in exploring the potential for RNAi as a strategy for pest management application comes from recent developments in our investigation into the genomic basis of toxin resistance in insects. This work suggests the vital importance of a family of genes found in all insects that we believe may function as a target for RNAi that would alleviate many of these stated concerns; these genes are directly involved in insect immunity, they are highly conserved throughout insects yet have enough sequence variability to allow for species-specific targeting, and many of these genes cause lethality when silenced using RNA interference. In our analysis, we highlight the potential of this family of genes in being leveraged towards pest management applications across arthropod species; and in our experimental study we target a member of this gene family in 2 species of fruit flies, Drosophila melanogaster and D. sechelia, as well as a target gene that has previously been shown to cause lethality when knocked down in fruit flies. We show that member genes in this family may be an effective target for the development of RNAi based bioinsecticidal approaches, with potential applications in a broad range of arthropod pests.
Speakers
CH

Chris Hayes

Wesleyan University
Co-authors
JC

Joseph Coolon

Wesleyan University
NA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Weed Control and Pest Management 2

1:00pm CDT

WCPM 1 - Prevalence of Aphid-Transmitted Potyviruses in Pumpkin and Winter Squash in Georgia, USA
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Viruses are a major biotic threat to cucurbit production in the southeastern United States. Many important cucurbit viruses are transmitted by whiteflies and aphids. Recent cucurbit virus research in Georgia is predominantly focused on whitefly-transmitted viruses (WTVs) and research is lacking on the aphid-transmitted viruses (ATVs) in cucurbit crops of Georgia. This study investigates the occurrence, prevalence, and emergence of ATVs in pumpkin and winter squash crops grown in a vegetable research farm at the University of Georgia-Tifton Campus during the fall season of 2022 and 2023. We screened plants for characteristic virus symptoms such as mosaic, mottling, yellowing, chlorotic spots, vein clearing, shoestring, upward curling, crumpling, blistering, and deformation of leaves. We collected symptomatic leaf samples of pumpkin and winter squash from the field. We processed the leaf samples for small RNA libraries for high-throughput sequencing (HTS) to identify ATVs present in the samples. We amplified viral genes for sanger sequencing and ran quantitative polymerase chain reactions (qPCR) for molecular validation of HTS results. HTS analysis revealed the presence of two ATVs, zucchini yellow mosaic virus (ZYMV) and papaya ringspot virus (PRSV). The qPCR results suggested a significant temporal shift in ATV’s abundance in these two crops. In 2022, PRSV and ZYMV incidence was observed in 56.25% and 31.25% pumpkin leaf samples. In winter squash, PRSV and ZYMV incidence was found in 50% and 32.14% leaf samples. Mixed infection of both viruses was at 28.12% for pumpkin and 25% for winter squash. In 2023, PRSV was not detected in pumpkins, and it was detected at a negligible level (0.62%) in winter squash. ZYMV was predominant in pumpkin (61.25%) and winter squash (42.50%). Phylogenetic analysis of ZYMV-encoded coat protein (CP) and helper component-protease (HC-Pro) suggested a close relationship with the European isolates. However, PRSV-encoded CP and NIa-VPg showed a close relationship with isolates from Australia, Papua New Guinea, Spain, and the United States. To the best of our knowledge, this is the first study to suggest single or mixed infections of ZYMV- and PRSV-infected pumpkin and winter squash in Georgia, USA. The findings of this study will serve as an important foundation for future research to understand the complex interactions between insect-transmitted viruses in cucurbit crops, which is vital for developing resistant cultivars and effective virus management strategies for commercial cucurbit vegetable crop production.
Speakers
NA

Nirmala Acharya

PhD student, University of Georgia
Co-authors
AS

Alvin Simmons

USDA ARS
NA
DG

David G. Riley

University of Georgia
NA
JC

Juan Carlos Diaz Perez

University of Georgia
MK

Manish Kumar

University of Georgia
NA
SB

Sudeep Bag

University of Georgia
NA
TM

Ted McAvoy

University of Georgia
NA
TC

Timothy Coolong

University of Georgia
Thursday July 31, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Weed Control and Pest Management 2
 
Friday, August 1
 

12:15pm CDT

CE 2 - Characterization and Control of Intumescence Development and Leaf Expansion for Capsicum annuum Production in Controlled Environments
Friday August 1, 2025 12:15pm - 1:00pm CDT
Capsicum annuum (pepper) is an emerging crop for controlled environment production that is susceptible to intumescence. Intumescence is a physiological disorder characterized by unrestricted cellular growth causing protruding lesions, ultimately leading to epidermal rupture. While the causative factor for this disorder remains unknown, water stress is commonly implicated and end-of-day (EOD) lighting has been identified as a potential strategy for mitigating intumescence development. However, the timing of pepper leaf area expansion and intumescence development as well as appropriate lighting strategies for their control have yet to be determined. The objectives of this work were to 1) determine the timing of leaf area expansion to better inform EOD or pre-dawn (PD) lighting applications for intumescence suppression; 2) quantify the impact of low-intensity lighting applications on pepper leaf morphology; and 3) determine the role of water stress in the occurrence of intumescence development for pepper. Pepper ‘Pot-a-Peño’, ‘Spicy Jane’, and ‘California Wonder’ were grown in 15-cm pots in a common greenhouse environment. For Objective 1, three weeks after transplant, one uniform leaf on each plant was tagged and plants were evaluated twice a day (0700 and 1900) for one week. For Objective 2, plants were subjected to 30-minute lighting treatments at an intensity of 25 μmol·m·−2·s−1 for two weeks provided at either EOD with blue (EOD-B; 447 nm), red (EOD-R; 659 nm), or far-red light (EOD-FR; 734 nm), or PD with blue light (PD-B; 447 nm). For Objective 3, plants were either maintained at 70% field capacity (control) or allowed a single event of reaching 40% field capacity prior to watering. Intumescence development was visually monitored twice a day (0900 and 1700) during the water stress event. For Objective 1, leaf area expansion was 46%, 34%, and 94% greater at night (1900-0700) compared to the day (0700-1900) for ‘Spicy Jane’, ‘Pot-a-Peño’, and ‘California Wonder’, respectively. For Objective 2, responses to lighting treatments were cultivar specific. For example, after two weeks, imaged leaf area was lowest under EOD-B for ‘Pot-a-Peño’ and greatest under EOD-FR for ‘Spicy Jane’. For Objective 3, intumescence development was observed on all cultivars subjected to water stress post returning to field capacity, with no incidence of the disorder for control plants. These results will help in the prediction of intumescence development for pepper produced in controlled environments and inform decisions regarding the timing of possible suppression methods to control this disorder.
Speakers
CD

Courtney Dunbar

Colorado State University
NA
Co-authors
JC

Joshua Craver

Colorado State University
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CE 2 - Comparative Evaluation of Hydroponic and In-Ground Onion (Allium cepa L.) Bulb Production
Friday August 1, 2025 12:15pm - 1:00pm CDT
Global demand for high-quality onion bulbs is rising, and there is a need for innovative, resource-efficient cultivation systems that stabilize production while mitigating soil-related limitations. Hydroponic cultivation systems, widely adopted for high-value crops, offer a promising alternative by enabling precise environmental control, optimized nutrient management, and reduced exposure to soil-borne diseases and weed competition. However, hydroponic methods for onion bulb production remain underexplored despite their success in other vegetable crops. Knowledge gaps exist in root-zone aeration dynamics, nutrient uptake efficiency, and the economic scalability of hydroponic systems for onion production. The objective was to determine the growth performance, bulb quality, yield, and financial feasibility of growing onion varieties across these systems. This study evaluated the comparative performance of three onion varieties: Candy Onion (intermediate-day), Walla Walla Onion (long-day), and Yellow Sweet Spanish Onion (long-day), grown under three hydroponic systems (Deep Water Culture (DWC), Kratky, and Drip Irrigation) along with inground production. The study followed a randomized complete block design with three replications. Key growth parameters, such as plant height, leaf number, and chlorophyll content, were recorded each week. Yield attributes, including bulb diameter, weight, total fresh and dry biomass, and harvest index, were analyzed after harvest. Moreover, bulb quality parameters such as total soluble solids (TSS), sulfur content, and pungency (pyruvic acid concentration) were assessed for consumer acceptability. The economic evaluation considered were the cost of cultivation, gross and net returns, and the benefit-cost ratio to determine the financial viability of hydroponic onion production for small-scale farmers in Kentucky. Despite the higher initial setup cost, hydroponic systems are projected to provide about 25% greater financial returns by reducing labor, pesticide, and fertilizer expenses, making them a sustainable solution for onion production. This study provided insight for farmers, researchers, and policymakers on integrating hydroponic technology for enhanced onion production, economic profitability, and sustainable agricultural practices. Further studies are necessary to validate the findings and guide best-practice recommendations for optimizing onion cultivation and supporting year-round production in Kentucky and beyond.
Speakers
SK

Srijana Kandel

Kentucky State University
Co-authors
JH

James Howard

Kentucky State University
NA
MC

Milon Chowdhury

Kentucky State University
SD

Siddhartha Dasgupta

Kentucky State University
NA
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CE 2 - Enhanced Airflow Improves the Growth and Morphology of the Common Biopharming Plant Nicotiana Benthamiana
Friday August 1, 2025 12:15pm - 1:00pm CDT
In recent years, a technology called biopharming, in which plants are used to produce pharmaceuticals, enzymes, and other high-value proteins, has been commercialized. Infiltrating the plants via the stomata, with genes that encode for these proteins, is a critical step in biopharming, but there is limited research on how to manipulate plant morphology to optimise this process. In this study, we investigated how increasing the airflow in a hydroponic system under vertical farm condition affects the growth and morphology of Nicotiana benthamiana, the plant most commonly used in biopharming. The plants were grown in a hydroponic system under vertical farm condition equipped with LED lights, with a photoperiod of 16 hours light/8 hours dark, and a photosynthetic photon flux density of 160-180 µmol m-2 s-1. The growing beds were filled with liquid fertilizer with an electrical conductivity of 1.6 dS cm-1 and a pH of 6.0 ± 0.5. Seeds of Nicotiana benthamiana L. were sown in urethane cubes and transplanted onto the bed. In a preliminary experiment, the fresh weight, plant height, and number of leaves were measured to investigate the growth of Nicotiana benthamiana under hydroponic conditions, and at 30 days after transplanting was determined to be the optimum number of growing days for use as a control regime in the present study. The enhanced air-flow treatment employed a constant wind speed of 0.1 to 0.2 ms-1, and strategically placed air ducts were used to ensure that each plant was evenly exposed. Seven plants (each had 8 to 10 leaves) were harvested, of which the first, third, and fifth leaves were used to measure leaf area and perform stomatal observations. Applying the enhanced air-flow treatment increased the fresh weight and average stomatal aperture of the plants by 1.2 and 1.1-fold, respectively. Leaf area was also increased markedly by 1.8-fold, compared to the control regime. The same results were obtained after three replications, indicating that the method is reproducible. These results suggest that airflow is an important environmental factor that could influence the efficiency of the infiltration process in biopharming.
Speakers
MB

Miyuki Baba

Meiji University
Co-authors
TI

Takashi Ikeda

Meiji university
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CE 2 - Evaluation of seaweed species on growth and physiological responses of tomato seedling exposed to heat stress
Friday August 1, 2025 12:15pm - 1:00pm CDT
Brown seaweed extracts, particularly Ascophyllum nodosum (AN), have shown beneficial effects on improving plant physiology, flower development and abiotic stress tolerance in various crops. However, limited research has been conducted on other seaweed species, such as Ecklonia maxima (EM) and Macrocystis pyrifera (MP). This study aimed to evaluate the thermotolerance of tomato (Solanum lycopersicum L.) seedlings in response to the application of three seaweed extracts, AN, EM and MP (1% v/v). ‘Big Beef’ tomato seedlings were root-drenched with seaweed extracts at transplanting and grown for 35 days with weekly foliar application in growth chambers set at 26/19°C (day/night, 16/8 h) for control and 33/26°C for mild heat stress treatments. Under heat stress, AN and EM treatments significantly increased shoot fresh weight by 12.5% and 10.8%, respectively, compared to the control, while MP treatments showed no significant differences. Also, seedlings treated with MP showed a numerical increase in chlorophyll fluorescence (Fv’/Fm’) by 12.8% and a reduction in leaf electrolyte leakage by 19.8% compared to the control under heat stress. However, no significant thermotolerance effects of seaweed extracts were observed in SPAD, net photosynthetic rate (Pn), pollen count and viability. In conclusion, the application of seaweed extracts provided differential response to thermotolerance benefits of tomato seedlings under mild heat stress conditions, with AN and EM enhancing shoot biomass and MP showing potential in mitigating physiological heat stress damage.
Speakers Co-authors
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

CE 2 - Extension of Tomato Production Season in Oklahoma using High Tunnel
Friday August 1, 2025 12:15pm - 1:00pm CDT
Open field cultivation of specialty crops such as tomato is challenging in Oklahoma due to unpredictable weather. In open fields, early planting of tomato seedlings during spring is hampered due to damaging chill weather conditions. On the other hand, higher temperatures during the summer months severely limit productivity. Furthermore, insect pests and disease pressure are high in open field conditions. As a result, the production cost per unit area is high. With the objective to develop cost effective and profitable production systems, tomato production in high tunnel was evaluated in Oklahoma. Six tomato cultivars including beefsteak and cherry types were evaluated in high tunnel at Langston University, OK during spring-summer season in 2023. High tunnel kept warm during the early seedlings establishment period and allowed early planting in spring by six weeks compared to open field cultivation. Similarly, by covering the roof with 45% shade cloth, a continuous harvest was achieved throughout the summer until third week of September. Study revealed the beefsteak tomato cultivars produced higher marketable fruit yield compared to cherry types. The marketable fruit yield of six evaluated tomato cultivars were in the range of 44.3 ton/ha - 77.12 ton/ha. Similarly, total soluble solid (brix %) content in high tunnel grown tomatoes were in the range of 5.1 % - 6.1%. Our study shows the early growth, season extension, and high yield of tomatoes due to use of high tunnels, and small - medium or limited resources farmers could benefit from it.
Speakers Co-authors
DJ

David Jones

Langston University
NA
KD

Kamal da Silva

Langston University
NA
LK

Leonard Kibet

Langston University
NA
RB

Richardson Boateng

Langston University
NA
RS

Rose Salomon

Langston University
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Growth Chambers and Controlled Environments 3

12:15pm CDT

CE 2 - Improving Bell Pepper Harvest with Inter-canopy Lighting in Northern Greenhouses
Friday August 1, 2025 12:15pm - 1:00pm CDT
Heating cost and low light limit greenhouse winter production in the north. Improved technologies can now support more efficient light delivery and interception. The greenhouse bell pepper cultivars Brocanto (yellow), Milena (orange) and Olly (red) were chosen to assess inter-canopy lighting with overhead high-pressure sodium irradiance. In addition to HPS, LED fixtures for in canopy placement were evaluated (GE current Arize® Integral). Plants were grown in a high-wire drip irrigation system using dutch bato buckets (17.7 L volume). The photoperiod was 16-h, day temperature 22 ± 2°C and reduced to 18 ± 2°C during the night. One or two horizontal LED bars were positioned and adjusted within 30 cm of the top of the plants throughout the study. In treatments with two LED bars, the bars were placed 30 cm (12 inches) apart. The intensity (400-700 nm) horizontally from the LEDs and measured at the location of the plant stems averaged 195 ± 30 µmol m-2s-1. Overhead HPS provided ~130 ± 20 µmol m-2s-1, 100 cm below the fixtures. Natural light was seasonally limited during the study. Seeds were sown on 29 Aug and one plant was transplanted into each container 50 d later (17 Oct). Lower leaves were removed as fruit ripened and the study was terminated at a plant height of ~180 to 200 cm. Colored peppers were first harvested 79 d from transplant (4 Jan) and the study was discontinued 8 weeks later. Plants grown with two LED bars produced higher yields than those with one bar or only HPS lighting. Olly produced 1.3 ± 0.09 kg under HPS, 2.2 ± 0.16 kg (one bar) and 3.2 ± 0.02 kg (2 bars) per plant while both Brocanto and Milena yielded 1.6 ± 0.19 kg under HPS, 2.3 ± 0.19 kg with one bar and 2.7 ± 0.09 kg (Milena) or 2.9 ± 0.10 kg (Brocanto) with two LED bars. The LEDs also increased the number of harvested peppers. For Olly, ten peppers were harvested on plants with only HPS and increased to 15 and 20 peppers with the LED bars. Six more peppers per plant were harvested with interlighting for Brocanto and Milena. The pepper size remained similar across treatments for Brocanto (197 ± 12.4 g) and Milena (170 ± 10.0 g). For Olly, the pepper size increased from 129 ± 6.0 g (HPS) to 161 ± 5.4 g with two LED inter-canopy bars.
Speakers
MK

Meriam Karlsson

University of Alaska Fairbanks
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

HSMP - Assessing micropropagated ginger cultivars and generations on growth and rhizome yield in a high tunnel
Friday August 1, 2025 12:15pm - 1:00pm CDT
Previous research demonstrated that tissue culture (TC) ginger grown in a high tunnel (HT) produced a higher yield than traditionally seed-sprouted ginger. This study further investigated any significant changes between generations of TC ginger in a HT. We studied 6 cultivars (BB, BG, BK, HY, KM, and MD) from two TC generations (TCF1 and TCF2). TCF1 seedlings were initially acclimated in a greenhouse and then transplanted into HT with TCF2 seed in June 2024. Randomized complete block design (RCBD) was used with 3 replications (blocks) per TC generation, 12 plots per block, 8 plants per plot (5 plants for HY) for a total number of 576 plants. Data collected: stem number
Speakers
GY

Guochen Yang

Professor and Graduate Program Coordinator, Oversight Coordinator for University Hemp Program, North Carolina A&T State University
Co-authors
DT

Daniel Tetteh

North Carolina A
NA
HT

Hannah Talton

North Carolina A
NA
JR

Julia Robinson

North Carolina A
NA
RM

Radiah Minor

North Carolina A
NA
TM

Trequan McGee

North Carolina A
WL

William Lashley

North Carolina A
ZM

Zipporia Moore

North Carolina A
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

HSMP - Growing Medicinal Plants in the Arid Southwest
Friday August 1, 2025 12:15pm - 1:00pm CDT
Water scarcity is one of the largest challenges to agricultural resilience in the increasingly arid Southwest. An important question is if expanding organic systems can support small farms to achieve the water demand reductions needed to preserve cultivation across agricultural valleys while also achieving overall agricultural resilience. Evaluating and selecting high value crops like medicinal plants for drought and heat tolerance might also lead to alternative crops that are suited for this environment. Organic systems and alternative crops could be critical for resilience, and our project’s goal was to identify crops and practices that are culturally relevant to small farmers in the southwest. High value crops with medicinal properties were cultivated using organic farming practices. A replicated split-field design trial of nine plant species including transplanted and seeded were grown for one season and were monitored for establishment, yield, and growth traits. First year data indicates that out of the nine species, only primrose (Oenethera biennis), tepary beans (Phaseolus acutifolius), spilanthes (Acmella oleracea), calendula (Calendula officinalis) and marshmallow (Althaea officinalis) were successfully established after transplanting seedlings in May 2024, however none of the direct seeded plants germinated. Spilanthes medicinal flowers were harvested from August till October, and calendula flowers were harvested from August till December. The perennials that over wintered successfully are producing new growth for a second season are calendula, and marshmallow. Our conclusions after the first year of establishment are that transplanting these high value crops is a better option than direct seeding. In addition, overwintering of perennial plants that have established are a viable option for farmers. For future work, amount of water needed for growth and quality of each species will be measured.
Speakers
avatar for Ivette Guzman

Ivette Guzman

New Mexico State University
Co-authors
CV

Ciro Velasco-Cruz

New Mexico State University
NA
CM

Connie Maxwell

New Mexico State University
NA
KL

Kevin Lombard

New Mexico State University
Kevin Lombard is a Professor of Horticulture and Research Director of the New Mexico State University Agricultural Science Center at Farmington, a 250 acre research farm uniquely located on the Navajo Nation. His research encompasses specialty crop evaluations including grapes, fruit... Read More →
KR

Kristen Ramos

New Mexico State University
RH

Robert Heyduck

New Mexico State University
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Herbs; Spices and Medicinal Plants
  • Subject Herbs Spices and Medicinal Plants
  • Poster # csv
  • Funding Source USDA NIFA ORG GR0007385
  • Funding Option OREl or ORG funded all or part of the research associated with this abstract

12:15pm CDT

HSMP - Impact Evaluation of Color Shading on Tissue Culture Ginger Growth, Rhizome Yield, and Physiology
Friday August 1, 2025 12:15pm - 1:00pm CDT
Ginger requires proper shading for optimum growth and rhizome yield. However, literature on individual color shading’s effects was limited. Under light stress, morphological and physiological traits change. Since 2023, we have studied how different color shading impacts growth, rhizome yield, and phytochemical composition of micropropagated ginger. Tissue culture ginger seedlings (cultivar Madonna) were transplanted in March in a greenhouse, then in June into 50-gallon grow containers, 12 plants per container in a 2:1 metro mix:compost substrate. A completely randomized design was used, with 12 color shade treatments and 12 replications per treatment at NCA
Speakers
WL

William Lashley

North Carolina A
Co-authors
DT

Daniel Tetteh

North Carolina A
NA
GY

Guochen Yang

North Carolina A
HT

Hannah Talton

North Carolina A
NA
JR

Julia Robinson

North Carolina A
NA
RM

Radiah Minor

North Carolina A
NA
TM

Trequan McGee

North Carolina A
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Herbs; Spices and Medicinal Plants

12:15pm CDT

HSMP - Pot Size Influences Establishment Of Vanilla planifolia In The USVI
Friday August 1, 2025 12:15pm - 1:00pm CDT
Vanilla planifolia, is a species of vanilla orchid native to Mexico. It is known specifically for its extract known as vanillin, which is used as a flavorful ingredient in food, beverages, cosmetics and perfumes. Vanilla plants, brought in from tissue culture, were planted in 10 or 15-gallon pots in media containing tree bark vermiculite and peat moss (1:1:1). Support for aerial roots involved rolling wired fencing and burlap together and placing eight-inch square mason blocks in each pot. The orchids were measured monthly for length and number of leaves. Results indicate that pot size had a slight influence on the plant growth of the vanilla plants. In the 10-gallon pots, the orchids grew to an average length of 126.5 cm, while the 15-gallon pots grew to an average length of 169.9 cm. The burlap also provided adequate space for the aerial roots to grow into over time. A 15-gallon pot, containing tree bark vermiculite and peat moss (1:1:1) was the most effective pot size to use for establishing vanilla plants. Vanilla p. can be established well in the subtropical climate of the USVI.
Speakers
DR

Daryl Richards Jr

Univ. Virgin Islands
NA
Co-authors
TZ

Thomas Zimmerman

University of the Virgin Islands School of Agriculture
XW

Xingbo Wu

University of Florida
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Herbs; Spices and Medicinal Plants

12:15pm CDT

HSMP - Sap Yield of Boxelder and Norway Maple and Syrup Production in Missoula, Montana
Friday August 1, 2025 12:15pm - 1:00pm CDT
Consumers increasingly demand locally sourced agricultural products and are willing to pay premium prices for novel and unique offerings. Maple syrup is a significant agricultural commodity in the United States, with a total market value of $158.67 million in 2023 and 5.86 million gallons produced in 2024 (USDA NASS, 2024). However, annual maple syrup production timing and yields vary widely by year and region, largely depending on climatic conditions. Expanding maple syrup production to western states could help meet the growing demand while providing additional income for landowners in areas where maple resources have not traditionally been utilized. Boxelder (Acer negundo) is native to the Intermountain West, while Norway maple (Acer platanoides) and other non-native maple species are common in urban and suburban areas of the region. Despite this, limited research has assessed the potential of these species for syrup production, highlighting the need for further study to support this emerging industry. On 10 Feb 2022, 11 Mar 2023, 4 Feb 2024, sap collection bags (18.93 L) were installed for tapping 20 boxelder (diameter at breast height (DBH) = 26.2 ± 3.7 cm) and 20 Norway maple (DBH = 29.5 ± 5.8 cm) trees in Missoula, MT. Sap was collected regularly to determine the yield for each tree from 11 Feb to 24 Mar 2022; 12 Mar to 9 Apr 2023; 5 Feb to 17 Mar 2024. Linear regressions were used to analyze cumulative sap yield over time, comparing data from the two maple species across three years. Maple trees exhibited varying sap yields across different years. In Missoula, MT, boxelder trees had seasonal sap yields of 9.21, 18.86, and 16.81 L during the 42-, 29-, and 42-day tapping periods, respectively. Norway maple trees produced seasonal sap yields of 28.38, 15.45, and 30.15 L during the 42-, 29-, and 42-day tapping periods, respectively. Boxelder trees yielded significantly more sap per day in 2023 compared with 2022, whereas Norway maple trees maintained relatively consistent daily sap yields across three years. Montana Mapleworks, the only commercially licensed maple syrup producer in the Intermountain West, produced 30, 20, 55, and 55 gallons of maple syrup in 2021, 2022, 2023, and 2024, respectively.
Speakers
YS

Youping Sun

Dr. Sun is an Associate Professor of Landscape Horticulture whose research focuses on understanding the whole-plant response to water stress, with an emphasis on water conservation, and developing protocols to produce native plants. He is the principal investigator for the ‘Climate... Read More →
Co-authors
DK

David Knudson

Montana MapleWorks
NA
XD

Xin Dai

Utah State University
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Herbs; Spices and Medicinal Plants
  • Subject Herbs Spices and Medicinal Plants
  • Poster # csv
  • Funding Source USDA Agricultural Marketing Service Acer Access and Development Program, USDA NIFA Hatch Project UTA01666, Utah Agricultural Experiment Station, and Center for Water-Efficient Landscaping

12:15pm CDT

HSMP - Sap Yield of Boxelder Trees in Montana, Utah and Wyoming
Friday August 1, 2025 12:15pm - 1:00pm CDT
The value of maple syrup production in the United States was $159 million in 2024, a 17% increase from the previous year. The timing and yield of maple syrup production vary widely between years and regions, depending heavily on climatic conditions. Expanding maple syrup production to western states could help meet the ever-increasing demand for syrup and provide additional income for landowners in areas where maple resources have not traditionally been utilized. Boxelder (Acer negundo), native to the Intermountain West, and other non-native maple species are common in urban and suburban areas of the region. However, limited research has assessed the potential of these species for syrup production in the Intermountain West. On 11 Mar 2023, sap collection bags (18.9 L) were installed to tap 20 boxelder trees (diameter at breast height (DBH) = 26.2 ± 3.7 cm, mean ± SD) in Missoula, MT. On 1 Mar. 2023, buckets (11.4 L) were installed to tap 30 boxelder trees (DBH = 42.6 ± 13.3 cm) in Logan, Providence, and Smithfield, UT. On 27 Feb 2023, buckets (11.4 L) were installed to tap 30 boxelder trees (DBH = 28.4 ± 8.8 cm) at the Sybille Research Site, Wheatland, WY. Sap was collected regularly to determine yield per tree from 12 Mar to 9 Apr 2023, in Montana; 2 Mar to 10 Apr 2023, in Utah; and 2 Mar to 1 May 2023, in Wyoming. Temperature data were obtained from the National Weather Service. A logistic model was used to fit the cumulative sap yield over time, comparing data from the three states. The location of boxelder trees significantly impacted sap yield. Seasonal sap yields were 18.9, 34.4, and 41.1 L per tree in Montana, Utah, and Wyoming, respectively, during the 29-, 41-, and 63-day tapping periods. Boxelder trees in Montana produced significantly higher daily sap yields than those in Utah and Wyoming, likely due to the shorter tapping season. With a similar tapping start date in Utah and Wyoming, boxelder trees in Utah began producing sap earlier than those in Wyoming, which may be related to temperature differences. However, boxelder trees in Wyoming yielded a greater total sap volume than those in Utah and Montana.
Speakers
YS

Youping Sun

Dr. Sun is an Associate Professor of Landscape Horticulture whose research focuses on understanding the whole-plant response to water stress, with an emphasis on water conservation, and developing protocols to produce native plants. He is the principal investigator for the ‘Climate... Read More →
Co-authors
AP

Abby Perry

University of Wyoming Extension
NA
BS

Brian Sebade

University of Wyoming
NA
DK

David Knudson

Montana MapleWorks
NA
JM

Jesse Mathews

Utah State University
NA
XD

Xin Dai

Utah State University
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Herbs; Spices and Medicinal Plants
  • Subject Herbs Spices and Medicinal Plants
  • Poster # csv
  • Funding Source USDA Agricultural Marketing Service Acer Access and Development Program, USDA NIFA Hatch Project UTA01666, Utah Agricultural Experiment Station, and Center for Water-Efficient Landscaping

12:15pm CDT

PNM 2 - Cranberry (Vaccinium macrocarpon) Associated Bacteria Increase Concentrations of Plant Growth Promoting Chemicals in vitro
Friday August 1, 2025 12:15pm - 1:00pm CDT
Bacteria associate with plants across diverse ecosystems, including agroecosystems, where they often benefit plant growth by increasing nutrient availability and mitigating stress, leading to improved productivity. Cranberries are clonally propagated perennials grown in highly acidic soils, where beneficial bacteria could help reduce the need for synthetic fertilizer and enhance nutrient availability, supporting plant health in the long term. We isolated 102 putative phosphorus-solubilizing bacteria in Pikovskaya’s Agar, and 112 putative plant hormone-synthesizing bacteria in Czapek Dox Agar, and assessed their metabolic function using specific assays: malachite green assay for phosphorus solubilization and a tryptophan-IAA assay for plant hormone synthesis. We found that of our 102 isolated phosphorus bacteria, 29 could solubilize more than 100 μM of phosphorus bound to iron within three days. Of the 112 hormone-synthesizing bacteria, 38 produced more than 20 μg/mL of the plant hormone indole-3-acetic acid (IAA) within three days. Next, we will assess these bacteria in soils associated with plants by inoculating cranberry plants under sterile and non-sterile environments and monitoring plant growth responses. This approach aims to reduce fertilizer costs and environmental impact by enhancing water quality and bolstering cranberry crop health.
Speakers
JR

Jessica Rohde

University of Wisconsin-Madison
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

PNM 2 - Effects of Varying Rates of Irrigation and Potassium Fertilization on the growth of Dendrocalamus asper in Florida.
Friday August 1, 2025 12:15pm - 1:00pm CDT
Bamboo is a perennial crop cultivated for different purposes such as production of edible shoots, timber and energy, and an effective medium for carbon sequestration. Bamboo production in the United States has gained significant interest over the past two decades due to its many benefits. Despite this progress, there is still a knowledge gap regarding nutrient and irrigation management recommendations for bamboo production for the unique Florida agroecological conditions. Fertilizer and irrigation are important crop management strategies for supplying plants with adequate nutrients and water for plant growth and productivity. Understanding the nexus between plants, water-use efficiency, and nutrient uptake is critical for sustainable bamboo production. A 4 x 4 factorial completely randomized design consisting of four irrigation rates (50% evapotranspiration (ET), 75% ET, 100% ET, and 125% ET) and four potassium application rates (0, 100, 200 and 300 lbs K/acre), replicated 5 times was established under greenhouse conditions. The results showed that different K levels did not have any significant effect on culm height and stomatal conductance. The 125% ET showed the highest culm height in the first month but in the following month, the 75% ET and 100% ET resulted in the greatest heights suggesting that reasonable culm height can still be achieved while conserving water. The 100% ET and 75% ET resulted in higher stomatal conductance compared to 125% ET and 50% ET. This implies that excessive irrigation and low irrigation rates may limit stomatal regulations and overall water use.
Speakers
LA

Labake Agunbiade

Citrus Research and Education Center, University of Florida
Co-authors
DK

Davie Kadyampakeni

Citrus Research and Education Center, University of Florida
NA
MN

Marcio Nunes

University of Florida
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

PNM 2 - Evaluating Nitrogen Mineralization and CO₂ Respiration Responses to Organic Amendments in Soil Incubation
Friday August 1, 2025 12:15pm - 1:00pm CDT
Understanding how organic amendments influence nitrogen (N) mineralization and carbon (C) respiration is essential for improving soil health and nutrient management in agroecosystems. This study, conducted by the Spring 2025 PSC 5560 Soil Analytical Techniques class at Utah State University, investigated the effects of various organic materials on soil N mineralization and CO₂ respiration through a controlled laboratory incubation. Six treatments were applied to a homogenized loamy soil collected from 0–30 cm depth: Miller compost, softwood compost, hardwood compost, wheat straw, feather meal, and an unamended control. Each treatment was mixed with soil and incubated in sealed mason jars under moist conditions for 35 days. CO₂ evolution was monitored via headspace gas sampling and analyzed with gas chromatography. Inorganic N (NH₄⁺ and NO₃⁻) was quantified on days 0, 7, 14, 21, and 35 through KCl extraction and spectrophotometric analysis. Results showed that respiration rates and mineral N release varied by treatment, reflecting differences in organic matter quality. Feather meal, with the lowest C:N ratio (3.8), released the highest levels of mineral N early in the incubation, although it did not fit the first-order kinetic model. Wheat straw, with a high C:N ratio (80.0), showed high cumulative CO₂ evolution, suggesting substantial microbial activity despite limited N mineralization. Compost treatments exhibited intermediate responses. Rate constants (k) for both C and N mineralization differed significantly among treatments, with feather meal and wheat straw showing the fastest rates for N and C, respectively. First-order kinetic modeling provided estimates for potentially mineralizable N (No) and C (Co), highlighting the variability in amendment quality. While No did not differ significantly across treatments due to high variability, k values indicated differing mineralization dynamics. These findings underscore the importance of selecting organic amendments based on crop nutrient demands and decomposition behavior. This work demonstrates the utility of laboratory incubation assays for evaluating compost quality and forecasting nutrient release. Future directions include field-scale validation and microbial community profiling to better understand the mechanisms behind observed differences in decomposition and nutrient cycling.
Speakers
IA

Isiaka Azeez

Utah State University
Co-authors
JN

Jeanette Norton

Utah State University
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Nutrient Management 1

12:15pm CDT

PNM 2 - Fertilizer nitrogen effect on cranberry fruit rot
Friday August 1, 2025 12:15pm - 1:00pm CDT
Nitrogen (N) is the most important element that influences both vegetative growth and fruiting in cranberry production. While many factors such as cultivar, age and vigor of vines are important, soil fertility is one of the most important factors impacting yield and fruit quality. The high fertilizer N used to support increased yield potential of new cranberry second generation hybrids can result in increased vegetative growth, thus creating a dense canopy resulting in a microclimate with high relative humidity (RH). Humid conditions during the warm summer months encourage the expression of cranberry fruit rot (CFR). The goal of this study was to determine the effect of N application rate on CFR occurrence in ‘Stevens’ and ‘Mullica Queen’ at the UMass Cranberry Station in East Wareham, Massachusetts. To measure biophysical data, we deployed micro climatic sensors in each treatment to measure ambient temperature and RH. Four N rates were used ranging from 46 kg N ha-1 to 112 kg N ha-1 in a replicated RCBD experiment. Fruit samples were collected at harvest from each treatment and used to measure total anthocyanin content (TAcy) by extraction with 0.2 N HCL. Cranberry biomass in both cultivars increased with increase in N rate and so did fruit rot. Fruit yield increased with N rate up to a point then it started to decline as rate increased. High N rate of 112 kg N ha-1 was associated with a low canopy temperature, and high RH compared to 46 kg N ha-1 in both cultivars. Total anthocyanins in the fruit decreased with N rate in both cultivars. Our results seem to suggest that high N rate encourage fruit rot expression.
Speakers
BM

Brian Makeredza

University of Massachusetts
Co-authors
GM

Giverson Mupambi

University of Massachusetts Amherst
PJ

Peter Jeranyama

University of Massachusetts Amherst
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

PNM 2 - Iron Biofortification Of Collard Greens: A Comparative Study To Analyze Efficiency Of Different Iron Fertilizers In Foliar Application
Friday August 1, 2025 12:15pm - 1:00pm CDT
Hidden hunger (micronutrient deficiency) is a global problem, with iron, zinc and selenium being the highly contributing members. Among these, iron deficiency is the most concerning. Deficiency of iron during pregnancy or in earlier childhood impairs the cognitive and behavioural development of children. Since the loss of iron from human body is minimal, iron deficiency occurs mainly because of insufficient intake. Therefore, increasing the iron content of vegetables (biofortification) is necessary, in order to meet the daily nutritional needs of humans. We conducted a field study in Fall 2024, comparing different iron fertilizers (FESO4, Fe-EDTA
Speakers
BJ

Bhupinder Jatana

Assistant Professor -Horticulture, Clemson University
Co-authors
AK

Arshdeep Kaur

Clemson University
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

PNM 2 - Phosphorus Fixation Dynamics in Quartz, Mixed Media, and Soils of Northeastern Florida and Its Effect on Snap Bean (Phaseolus vulgaris) Growth and Yield
Friday August 1, 2025 12:15pm - 1:00pm CDT
Phosphorus (P) is a macronutrient essential for plant growth and development, involved in root formation, photosynthesis, and metabolic processes such as energy transfer, cell division and others. Phosphorus leaching is a crucial concern due to nutrient loss and eutrophication of water bodies. This study aimed to evaluate growth performance, yield, and phosphorus uptake efficiency of Snap Bean (Phaseolus vulgaris) across three different growth mediums: (1) Quartz Sand, (2) 50% Quartz 50% Soil, and (3) Soil. Additionally, bioavailable orthophosphate content in leachates and the impact of different phosphorus application rates on crop productivity were assessed. Two phosphorus application treatments: (1) a single application of 120 lbs/acre and (2) a split application of 20 lbs/acre applied weekly over a period of six weeks. Leachate samples were collected and analyzed weekly to determine phosphorus availability trends. Growth parameters such as plant height, leaf chlorophyll content, and yield parameters including the number of pods per plant and pod weight were recorded to evaluate crop performance. Phosphorus uptake efficiency was assessed to determine how different growth media and fertilization strategies influence nutrient utilization. Results showed that Quartz retained the least phosphorus, leading to higher orthophosphate concentrations in leachates. The mixed medium of 50% Quartz 50% Soil exhibited intermediate retention, while Soil treatments retained the most phosphorus, resulting in the lowest leachate orthophosphate levels. Overtime, the split application of 20 lbs/acre provided more stable phosphorus availability compared to the single high-dose application, which led to an initial peak followed by a significant decline. Plants under mixed media and split application present the higher crop performance and yield. These findings highlight the role of growth medium composition and fertilization strategy in phosphorus retention and availability. The study suggests that combining appropriate growth media and phosphorus application rates can maximize Snap Bean productivity while minimizing environmental impacts. Further research should explore the interactions of these factors under diverse environmental conditions to develop best practices for phosphorus management and sustainable agriculture.
Speakers
EM

Elena Maximo

University of Florida
Co-authors
GL

Guodong Liu

University of Florida
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Nutrient Management 1

12:15pm CDT

PNM 2 - Plant Growth and Physiological Responses to Biostimulant Tetradesmus deserticola of Lettuce in Containerized Silica Sand
Friday August 1, 2025 12:15pm - 1:00pm CDT
Sustainable fresh vegetable crop production using lunar regolith as a growth media is critical for establishing large-scale space farming on the lunar surface. While lunar regolith contains essential mineral nutrients, it lacks organic carbon and nitrogen, which limits mineral bioavailability and nutrient-holding capacity. Tetradesmus deserticola is a terrestrial, photosynthetic algae that self-propagate in minimal media using light and carbon dioxide. It contributes organic matter to marginal soils through photosynthesis and has been used as biostimulant. The objective of this research was to evaluate the effects of T. deserticola inoculation on the growth, physiological, and morphological responses of lettuce (Lactuca sativa) plants at ammonium nitrate rates of 0, 1, and 2 mM, and 15N–3.9P–10K controlled-release fertilizer (CRF) at rates from 0 to 8.4 g·L-1 in a containerized silica sand media. Lettuce seeds were sown in the containerized media and inoculated with dried T. deserticola. After seed emergence, plants were irrigated with nitrogen-free Hoagland’s solution supplemented with ammonium nitrate or top-dressed with CRF at rates from 0 to 8.4 g·L-1. Electrical conductivity (EC), pH, and nitrate-nitrogen concentrations in leachate were recorded weekly. Gas exchange rates were measured, and plants were harvested 59 days after sowing to assess growth and morphological responses. Nitrate-nitrogen concentration and EC in leachate increased with increasing levels of ammonium nitrate in the Hoagland’s solution and CRF rates. In addition, the plant growth index and relative chlorophyll content of leaves was enhanced with the increase of ammonium nitrate levels and CRF application rate. Compared with non-inoculated plants, lettuce inoculated with T. deserticola showed higher plant growth index and relative leaf chlorophyll content when receiving nitrogen-free Hoagland’s solutions with 0, 1, and 2 mM of ammonium nitrate. However, the inoculation of T. deserticola did not increase plant growth index and relative chlorophyll content for plants treated with CRF. The T. deserticola inoculation enhanced growth and physiology of the Romaine lettuce at ammonium nitrate levels ranging from 0 to 2 mM. However, T. deserticola did not affect plant development when the plants were treated with CRF. Under the conditions of this study, the effects of T. deserticola inoculation on plant growth differed between fertilizer management.
Speakers
NA

Nintya Avriantari

University of Wyoming
Co-authors
DC

David Claypool

University of Wyoming
DH

Drea Hineman

University of Wyoming
NA
GD

Gioia D. Massa

NASA Kennedy Space Center
NA
JC

Ji-Jhong Chen

University of Wyoming
JS

John S. Oakey

University of Wyoming
NA
MF

Mady Fagan

University of Wyoming
NA
SB

Scott B. Jones

Utah State University
NA
SM

Shawna M. McBride

University of Wyoming
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Plant Nutrient Management 1
  • Subject Plant Nutrient Management
  • Poster # csv
  • Funding Source NASA EPSCoR Rapid Response Research (R3) Grant#: WY-80NSSC24M0162, University of Wyoming Science Initiative Seed Grant: Living Materials, Wyoming Space Grant Consortium Faculty Research Initiation, University of Wyoming College of Agriculture, Life Sciences, & Natural Resources, Office of Research and Economic Development Designated, Department of Plant Sciences. Y-Cross Scholarship. United States Department of Agriculture National Institute of Food and Agriculture (NIFA) Plant Sciences Hatch Funds (WYO-631-22)

12:15pm CDT

PNM 2 - Response of young Dendrocalamus asper to variable rate phosphorus fertilization dynamics in a controlled environment
Friday August 1, 2025 12:15pm - 1:00pm CDT
Fertilizers are commonly applied to bamboo to enhance quality and productivity. Bamboo is widely used for consumption, construction, and fabrication. Bamboo production has expanded to 690 ha in Florida. However, there are no clear phosphorus (P) fertilization guidelines, despite P playing a crucial role in plant growth and production. This study evaluated the effects of varying P rates on Dendrocalamus asper under greenhouse conditions. Two trials were conducted on one-year-old bamboo plants at different P rates (0, 22.4, 44.8, and 89.6 kg P ha-1) for five months in 2023 and 2024. Growth, physiological parameters, and biomass accumulation were assessed. Data were analyzed using one-way analysis of variance with a linear mixed model for repeated measures. Results showed that P fertilization significantly influenced bamboo growth, culm biomass accumulation, culm production, and chlorophyll content across both years. The highest biomass in culms, total biomass, number of culms, and clump height occurred at 22.4 and 44.8 kg P ha-1 in 2023 and 2024, respectively. Higher P rates (44.8 and 89.6 kg P ha-1) increased below-ground biomass. The highest P rate (89.6 kg P ha-1) increased soil P by 74% in 2023 and 84% in 2024 from the initial concentration. Soil P positively correlated with Ca in 2023 and K in 2024. Although no clear optimal P rate was established, moderate P application (22.4-44.8 kg P ha-1) benefited young bamboo growth. These findings provide a foundation for developing P fertilization guidelines in Florida. Further field-scale studies are needed to determine the optimal application rate.
Speakers
avatar for CYRUS JANUARIE

CYRUS JANUARIE

Graduate Research Assistant, UNIVERSITY OF FLORIDA
Soil nutrient managementStatistical data analysisField research 
Co-authors
DK

Davie Kadyampakeni

University of Florida
NA
JV

Joao Vendramini

University of Florida
NA
LS

Lakesh Sharma

University of Florida
Dr. Lakesh Sharma is an assistant professor of soil fertility and sustainable agriculture at the University of Florida in Gainesville, FL. Lakesh has been farming since he was a child on his own farm. His academic school journey started in 2000. He is currently working on nutrient... Read More →
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

VCM 3 - Agrivoltaics: How Solar Panels Affect Summer Squash 'Multipik' Production
Friday August 1, 2025 12:15pm - 1:00pm CDT
Agrivoltaics is a production system that allows for dual land use through the deployment of solar panels for energy production while maintaining agricultural production underneath and/or between solar panels. This study was conducted at the Alliant Solar farm at Iowa State University, Ames, IA. Treatments included summer squash (Cucurbita pepo ‘Multipik’) grown between and outside (open-field) the solar panels. For the solar panel treatment, two raised beds with white plastic mulch were created on each side of the single-axis solar tracking panels. Each bed was 19.5m long. Two beds on each side were spaced 1.5m (center to center). Two rows of open-field summer squash served as the control treatment. Control beds were 6.7m long. All treatments were replicated three times. Summer Squash was transplanted on May 31, 2024 at 0.6m spacing, single row. HOBO soil temperature, air temperature, and light intensity sensors were installed on the same day. Mustang MAXX® AND PyGanic® were sprayed to manage spotted cucumber beetles and squash bugs. A total of 31 harvests occurred throughout the season every 2 to 3 days between July 1 and September 20. Plants between the solar panels yielded 49.5 kg per row while the open-field yielded 36.5 kg, a 26% increase in the solar treatment. Between June, July, and August, the air temperature was cooler than the control on average by 3 degrees Fahrenheit while the soil temperature was 2 degrees Fahrenheit cooler. Light intensity decreased by 34% under the solar panels. Based on first year results, summer squash shows promise within an agrivoltaics production system. More data will be collected in the coming years on plant growth characteristics, soil moisture, and postharvest quality of summer squash.
Speakers
RW

Raymond Wilmes

Iowa State University
Co-authors
AN

Ajay Nair

Iowa State University
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Vegetable Crops Management 3

12:15pm CDT

VCM 3 - Assessment of Rhizoma Perennial Peanut under Agrivoltaic System in Georgia
Friday August 1, 2025 12:15pm - 1:00pm CDT
Solar energy production has been rapidly expanding in the Southeast; in Georgia alone, acreage has increased from under 500 to over 25,000 acres in the last decade. Agrivoltaics and ecovoltaics have emerged as strategies to ease land competition between Large-Scale Solar (LSS), agriculture, and natural resources. Agrivoltaic systems co-locate solar panels with row crops or livestock agriculture, thereby “stacking” the production of energy and agricultural products on the same land. LSS sites are typically seeded with Bahia and Bermuda grasses to control erosion while vegetation height is maintained by mechanical mowing. However, one of the most common and successful agrivoltaic applications is grazing livestock – typically sheep, which benefit from a high-protein diet, such as a legume. Rhizoma Perennial Peanut (RPP) is an emerging forage crop grown predominantly in southern areas with sandy soils and permissive winter temperatures. Most LSS are located in mid- and South Georgia where soils may have higher clay content. We set out to explore feasibility of RPP as a land cover under solar system in South Georgia. RPP ‘EcoTurf’ was planted between solar panels and outside the solar array. We implemented two experimental treatments: three seeding rates (100, 140, or 160 bushels/acre), and fertilization at planting or at mid-season. We measured canopy spread, leaf chlorophyll content and fluorescence. We also counted blooms and made aerial sweeps to assess arthropod abundance and diversity. In addition to being a potential grazing source, RPP matures well below the leading edge of the solar panels, thus eliminating the need for mowing even without livestock. If left uncut, RPP produces attractive yellow blooms which may serve as floral resources for pollinators and other beneficial insects. In this way, RPP can provide ecosystem services, including biodiversity and wildlife habitat, a potential win-win opportunity for LSS development and natural ecosystems in the Southeast USA. Given the selection of low growing ground cover that requires less frequent mowing (or no mowing), the implementation of RPP could also lead to lower inputs and reduced operation costs of the LSS in the long term.
Speakers
SP

Simona Pitardi

University of Bari
NA
Co-authors
JS

Jason Schmidt

University of Georgia
NA
LL

Leynar Leyton

University of Florida
SP

Svoboda Pennisi

University of Georgia
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

VCM 3 - Developing Artichoke as a New Winter Crop in a Subtropical Climate
Friday August 1, 2025 12:15pm - 1:00pm CDT
Artichoke (Cynara cardunculus var. scolymus) belongs to the sunflower family and is cultivated for its edible flower buds. Being adapted to Mediterranean climates, artichoke requires adequate winter chilling for flower bud induction but is susceptible to freeze damage, limiting its commercial production in the United States almost exclusively to California. In tropical and subtropical climates, despite minimal freeze risk, insufficient winter chilling presents a major constraint to artichoke production. This study was initiated in 2015 to evaluate the potential for establishing artichoke as a viable winter crop in Florida. First, we developed a protocol for artificial bud induction using the plant hormone gibberellic acid (GA₃). When applied at the optimal rate and developmental stage, GA₃ effectively promotes bud formation independently of natural winter chilling. From 2016 to 2020, we evaluated seven cultivars based on earliness, yield, and bud quality and selected two promising cultivars, ‘Imperial Star’ and ‘Green Queen’. We also evaluated planting dates from early September to early December and determined that early to mid-October is the optimum planting window, yielding up to 17.3 and 11.8 t ha–1 for ‘Imperial Star’ and ‘Green Queen’, respectively. From 2022 to 2025, we tested additional 16 hybrids and identified three promising cultivars, ‘20-063’, ‘22-017’ and ‘23-057’. Our current artichoke production guide includes recommendations on GA3 application, cultivars, planting configurations, plastic mulch, and basic pest management. In 2023, artichoke was grown on about 12 hectares in Florida using this production guide. Ongoing research focuses on assessing nutritional values and postharvest quality to enhance the viability of artichoke as Florida’s new winter crop.
Speakers
AK

Avninder Kaur

University of Florida
Co-authors
SA

Shinsuke Agehara

University of Florida
TL

Tie Liu

University of Florida
WW

Weining Wang

University of Florida
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

VCM 3 - Evaluating the impact of intercropping vegetables and cut flowers on economics and conservation biological control
Friday August 1, 2025 12:15pm - 1:00pm CDT
Vegetable production is highly susceptible to yield and quality loss due to insect pests leading to increased management intensity. Use of conservation biological control practices such as intercropping offers potential benefits to environmental sustainability while also maintaining economic profitability. This study was conducted to evaluate the impact of intercropping bell pepper (Capsicum annuum ‘King Arthur’) with cut flowers including zinnia (Zinnia elegans ‘Benary’s Giant White’), sunflower (Helianthus annus ‘Soraya’) and dianthus (Dianthus caryophyllus ‘Chabaud Benigna’). Data collected included insect and disease pressure ratings, marketable and unmarketable yield, economic return, and insect population diversity. Pepper yield was positively impacted in treatments including dianthus, while economic return was greatest where bell pepper was intercropped with zinnia.
Speakers
AI

Alliance Igiraneza

University of Nebraska-Lincoln
NA
Co-authors
KK

Katja Koehler-Cole

University of Nebraska-Lincoln
NA
RS

Ronald Stephenson

Plant Sciences Hall
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Vegetable Crops Management 3

12:15pm CDT

VCM 3 - Exploring Regenerative Vegetable Systems on the California Central Coast
Friday August 1, 2025 12:15pm - 1:00pm CDT
Regenerative farming practices focus on improving soil health and creating resilient agricultural systems, offering long-term sustainability and environmental benefits compared to conventional organic methods. This study investigated the effects of an in-season cover cropping practice on insect and weed density and crop yield on California’s Central Coast. Two field trials compared regenerative and standard organic systems. Weed populations were assessed biweekly using 50cm x 50cm quadrats, while insect populations were monitored with yellow sticky cards and direct counts. Yields were measured in kilograms per hectare and categorized as fresh marketable, processed marketable, and unmarketable. Both treatments were planted on 80-inch beds using certified organic inputs and methods. The regenerative treatment featured a cereal cover crop in the bed center, with two rows of transplants on either side. The first trial took place at Cal Poly San Luis Obispo Organic Farm (Jan–May 2024) with cabbage and sudan grass (three replicates). The second was at a grower’s field near San Ardo, CA (Feb–June 2025) with sweet baby broccoli and ryegrass (four replicates). In Trial 1, weed coverage was not significantly different between treatments (19.6 ± 5.3% for standard organic, 14.9 ± 0.7% for regenerative). Flea beetles were the most common pests, with no significant differences in pest densities (370.67 ± 24.17 vs. 403.11 ± 23.27). Predator and parasitoid insect counts were similar, and no pollinators were observed. Total yields were 5730.91 ± 5688.32 kg/ha for standard organic and 4929.50 ± 4890.27 kg/ha for regenerative. Fresh marketable yields were comparable (3169.77 ± 3139.50 vs. 3237.02 ± 3204.51). Although both treatments experienced high weed pressure, regenerative plots had fewer weeds, suggesting the grass cover crop may aid suppression. Insect density was numerically higher in regenerative plots, which also had slightly lower yields—possibly due to the grass strip. In the second trial, effective weed management led to no observed weeds. Higher insect densities were found in regenerative plots. These findings indicate that regenerative practices, such as incorporating a grass cover crop, may influence weed suppression and insect activity, though they may also slightly reduce yields. Continued research is needed to better understand these trade-offs and optimize regenerative system design for both productivity and ecological benefits.
Speakers
UO

Una O'Connell

Cal Poly SLO
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Vegetable Crops Management 3

12:15pm CDT

VCM 3 - Humic Acid: Effects on Nutrient Uptake and Photosynthetic Capacity
Friday August 1, 2025 12:15pm - 1:00pm CDT
The current marketing of humic acid products to potato growers indicates that incorporating these materials at planting will lead to improved nutrient uptake, increased soil health, vigorous growth, and increase in yields. Humic acids are composed of long chained, organic molecules that can be naturally or industrially made. In 2024, the in-furrow application of humic acid was evaluated in Pennsylvania on the cultivar ‘Lehigh’. Plots were evaluated for nutrient uptake and photosynthetic capacity as impacted by use of Black Label Zn, Duo Maxx, Fertiactyl, Humi-Flex FA, Hydra-Hume, and Monty's Carbon. Preliminary data show no statistically significant difference between applied products and an untreated control for macro and micronutrients analyzed by plant tissue sampling. No statistically significant differences were observed for stomatal conductance, chlorophyll fluorescence, or electron transport rate. These preliminary data suggest that there is no positive return on investment with using humic acid products in-furrow at the time of planting. As part of a three-year study, data from 2023 supported a similar conclusion.
Speakers
RS

Ronald Stephenson

Plant Sciences Hall
Co-authors
DL

Donald Lee

University of Nebraska-Lincoln
NA
JP

Jonathan Price

University of Nebraska-Lincoln
NA
MS

Meghan Sindelar

University of Nebraska-Lincoln
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

VCM 3 - Impact of Soil Fertility Amendments and Cover Cropping on Soil Health in Vegetable Production
Friday August 1, 2025 12:15pm - 1:00pm CDT
Organic farming systems face inherent limitations in nutrient management, particularly when plant nutrient demand exceeds available supply. This multi-year study examined the effectiveness of various soil amendment strategies and cover cropping systems on soil health indicators and pepper yields across three Purdue Agriculture Research Centers in Indiana (Wanatah, Lafayette, and Vincennes). Our experimental design compared conventional, organic, and mixed soil amendment approaches using standardized nutrient targets across treatments. Soil amendments included conventional and organic nutrient sources, plant and manure-based, or mixed (leaf mold and manure) compost applications. Summer cover crops (mustard for biofumigation, sunn hemp for nitrogen fixation, and sorghum-sudangrass for biomass production) were planted in Year 1, while a mix of cereal rye and hairy vetch served as fall/winter cover crops throughout the study. Buckwheat was seeded in bed row middles during Years 3 and 4. Soil health was assessed using the Haney test on composite samples from fifteen 15 cm deep soil cores per experimental unit. Initial challenges from nutrient losses due to heavy rainfall and significant weed pressure in 2021 necessitated management adaptations, including preplant herbicide application and plastic mulch protection of soil amendments. Soil health metrics improved dramatically at the Lafayette location in 2022 (67-202% increase) but showed minimal improvement at Wanatah and Vincennes. By 2023, average soil health declined by 14% across sites, primarily due to decreased water-extractable organic nitrogen (WEON) and slight reductions in water-extractable organic carbon (WEOC), resulting in reduced microbial activity. Our findings demonstrate that summer cover crops combined with fall/winter cover crops produced comparable benefits to high-rate compost applications for both crop yield and soil health improvements. However, high compost applications, particularly at Lafayette, led to extremely high P₂O₅ levels that required adjustment of fertility management. Soil respiration emerged as a reliable predictor of overall soil health, with statistical analysis indicating that a 10-point increase in soil respiration corresponded to soil health improvements of 1.0, 1.1, and 1.3 units at Wanatah, Lafayette, and Vincennes, respectively. This research provides evidence-based guidance for integrated soil fertility management that balances immediate crop nutrient needs with long-term soil health objectives, offering sustainable strategies for both organic and conventional production systems.
Speakers
PL

Petrus Langenhoven

Purdue University
Co-authors
NS

Nathan Shoaf

Purdue University
Nathan Shoaf earned his M.S. in Horticulture and Landscape Architecture from Purdue University, specializing in horticulture and soil microbial ecology. With over two decades of experience, he has expertise in urban farms and specialty crop production, fostering impactful collaborations... Read More →
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Vegetable Crops Management 3

12:15pm CDT

VCM 3 - Soil Health in Fitotolodo’s in the Colca Valley Peru: Impact of Time and Management Practices
Friday August 1, 2025 12:15pm - 1:00pm CDT
The Colca River Valley is an important agricultural and touristic region in southern Peru ranging from 2,500 to 4,000 meters above sea level. Many of the challenges associated with farming in this hyper-arid region were overcome through the establishment of unique terraces on the steep hillsides centuries ago, which have improved soils and help retain water and heat. However, the cold temperatures in this region prevent farmers from growing many warm-season crops such as tomatoes and zapallo, which bring high returns in larger cities like Arequipa. Fitotoldos are temporary greenhouse like structures created using earthen bricks and a plastic covering. Much like high tunnels or polyhouses in the U.S., these structures can help extend the growing season and allow farmers to grow new warm season crops that bring higher returns. In the past five years, many initiatives from the Peruvian government and several non-governmental organizations have provided funds to establish fitotoldos in the Colca River Valley. However, the farmers recieved little guidance on how to effectively manage soils and crops in these structures. This has led to poor quality crops and the abandoment of many of these structures. To help overcome this challenge, we conducted a survey of soils and farming practices in these structures. Soil samples were subject to several tests to quanitify soil physical, chemical, and biological properties and determine how these properties were impacted by the farmer’s management practices. Results were shared with farmers and used to design training programs that will help local farmers better manage these structures and their crops over the long-term.
Speakers
LH

Lori Hoagland

Purdue Univ - West Lafayette, IN
Co-authors
DM

Dennis Macedo

University of Saint Augustine
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Vegetable Crops Management 3

12:15pm CDT

VCM 3 - Timing of Seeding is an Effective Ecological Weed Control Strategy for Edamame: Preliminary Results from a Greenhouse Study
Friday August 1, 2025 12:15pm - 1:00pm CDT
Edamame [Glycine max (L.) Merr.] is a specialty crop with untapped potential in the Midwest. Adoption of edamame in the Midwest has potential due to similarities in production practices to grain soybean and the potential for higher return per acre. Additionally, USA is experiencing expanding market opportunities and rising consumer interest in locally grown edamame. Despite these benefits, limited research exists on suitable edamame varieties for this region, and effective weed control remains crucial for Midwest edamame adoption. Our research aimed to identify currently available varieties of edamame with rapid germination response, with and without weed competition. Six readily available varieties of edamame (Chiba green, Sayamusume, BeSweet 292, Midori Giant, Tohya, and Karikachi) were grown with and without velvet leaf (Abutilon theophrasti Medik.) competition. Weed seeds were planted using three timing treatments: two weeks before edamame planting (PRE), simultaneously with edamame (SIM), and two weeks after edamame planting (POST). Two control treatments without weeds (‘Control 1’ and ‘Control 2’) were also incorporated. This study was designed as an RCBD split-plot design with three replications, with variety as the main plot and timing of weed competition as the split-plot under greenhouse conditions. Edamame emergence rate, plant height, LA (leaf area), and dry weight for both edamame and weed were measured. At 5 and 9 DAS (days after seeding), edamame emergence was significantly higher without weed competition (71.76% and 95.37% in ‘Control 2’ treatment) compared to when weeds had a head start (46.53% and 72.69% in ‘PRE’ treatment). At 9 DAS, edamame emergence was significantly higher when edamame had a head start (93.06% in ‘POST’ treatment) compared to when weeds emerged first (72.69% in ‘PRE’ treatment). In terms of emergence rate, Karikachi showed superiority (78.89%) compared to Midori Giant (39.17%) at 5 DAS, but there was no difference among varieties at 9 DAS. Timing of weed planting affected edamame height, LA, and edamame dry weight. Edamame height and dry weight were reduced by 11.67% and 5.71 % by weed competition when weeds were planted two weeks earlier than edamame. Edamame competition significantly reduced weed dry weight by 93.94% when weeds were planted two weeks later and by 84.85% when planted the same day, compared to weeds established two weeks before edamame. Weed competition reduced edamame LA by 29.189 cm² when weeds were planted simultaneously with edamame compared to two weeks after edamame planting (241.582 cm² in ‘SIM’ vs 270.771 cm² in ‘POST’).
Speakers
MM

MD MOYEED HASAN TALUKDER

Purdue University
Co-authors
MB

Moriah Bilenky

Purdue University
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Vegetable Crops Management 3

12:15pm CDT

WCPM 2 - Chemical Mowing: A Prospective Sustainable Weed Management Strategy For Christmas Tree Production
Friday August 1, 2025 12:15pm - 1:00pm CDT
The Christmas tree industry is a million-dollar industry in the United States. Like all crop production systems, Christmas trees are also affected by weeds. Noxious plants such as Canada thistle, horsenettle, poison ivy, and pokeweed compete with Christmas trees not only for light, space, moisture, and nutrients but also harbors pests and pathogens. Additionally, they attract wild animals like deer which are detrimental to this tree production. Large weed species create significant difficulty in crop management practices and intercultural operations. Christmas trees are more prone to damage from weeds in the seedling beds and the first three years in the field after transplantation. Young trees often suffer moisture stress due to weeds and succumb to drought. Once established, weeds are very difficult to manage, particularly the management of vining and woody weeds without damaging the Christmas trees is almost impossible. In severe cases, they devastate the whole production system and cause unbearable losses to growers. The most commonly used method for weed management in Christmas tree production is the application of chemical herbicides. Herbicides can cause severe injury to Christmas trees if applied inappropriately and can cause environmental issues. In addition, repeated use of the same herbicides can trigger herbicide resistance in weed species. Chemical mowing is a weed control technique that has been implemented in Florida citrus production, and it has been successful. Chemical mowing consists of the use of sublethal rates of postemergence herbicides in conjunction with mechanical mowing. However, there is no data or research showing whether this can be implemented in the flat land Christmas tree production system. How can chemical mowing help prevent weed shifts and herbicide resistance from developing? These significant knowledge gaps have led to the development of this master’s research proposal. Hence the objectives of this research proposal are: Developing a chemical mowing strategy in Christmas tree production by evaluating the efficacies of three different postemergence herbicides at lower rates combined with mechanical mowing; Evaluating their phytotoxic effects on two different varieties of Christmas trees; Determining the optimal moisture range for efficient chemical mowing. The preliminary results showed that an effective weed control can be achieved in Christmas tree production at sublethal rates of postemergence herbicides. Also, no significant injury was observed in Christmas trees due to chemical mowing.
Speakers
ST

Shailendra Thapa

Michigan State University
Co-authors
DS

Debalina Saha

Michigan State University
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

WCPM 2 - Evaluating Emergence of Two Container Nursery Weed Species Through Rice Hull Mulch When Applied at Two Different Growth Stages
Friday August 1, 2025 12:15pm - 1:00pm CDT
Weeds are a continual, year-round threat to nursery profitability due to their negative impact on crop growth, quality, and marketability, as well as the increased need for pesticide use and manual labor. In recent years, many nurseries have adopted parboiled rice hulls mulch as an alternative to herbicides. Rice hulls are lightweight, easy to apply, transport, and hydrophobic, making them an effective mulch for container-grown ornamentals. Previous research has demonstrated that rice hulls can provide excellent weed suppression when applied at sufficient depths. However, mulch is often applied 2–3 weeks after potting, during which time weed seedlings may emerge. There is a lack of research on the survivability of small weed seedlings that emerge prior to mulch application, and whether mulching at this stage could serve as a viable alternative to manual weeding. Therefore, this study aimed to evaluate the effect of rice hull mulch depth on the emergence of eclipta (Eclipta prostrata) and phyllanthus (Phyllanthus tenellus) at two growth stages. Square 1.7 L nursery containers were filled with a substrate composed of pine bark, peat, and sand (80:10:10 v: v: v), amended with dolomitic lime (target pH 5.5) and a controlled-release fertilizer [Osmocote® Plus 21-4-8, (8–9 month)]. Containers were seeded with either eclipta or phyllanthus which were allowed to grow, reaching one of two developmental stages including the cotyledon to 1-leaf or 2–4 leaf stage, using staggered sowing dates. Seedlings were then thinned to five weed plants per container, and mulch was applied at depths of 0, 0.6, 1.3, 2.5, and 5 cm. Weed emergence was recorded at 2, 4, 8, 12, and 16 weeks after mulching, and shoot dry weight was measured at trial conclusion. All containers were maintained in a greenhouse and received 0.7 cm of daily overhead irrigation. Results indicated that weed seedling survival and final biomass decreased as mulch depth increased. Rice hull mulch at depths of 1.3 cm or greater significantly reduced emergence and biomass of both weed species, providing close to 100% control. These findings suggest that rice hull mulch can be successfully applied 2–3 weeks after potting if a minimum depth of 1.3 cm is used, offering growers a flexible and effective non-chemical weed control strategy.
Speakers
CM

Chris Marble

University of Florida/IFAS
Co-authors
YK

Yuvraj Khamare

The University of Florida
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Weed Control and Pest Management 1

12:15pm CDT

WCPM 2 - Gemini Granular as a Pre-Emergent Weed Control Option for Eupatorium purpureum ‘Gateway’
Friday August 1, 2025 12:15pm - 1:00pm CDT
Mississippi State University’s South Mississippi Branch Experiment Station works with the IR-4 project to conduct trials and collect data to facilitate EPA registration of pest management products important in production of specialty crops. During the 2024 growing season, Gemini Granular was evaluated for phytotoxicity and pre-emergent weed control for Eupatorium purpureum ‘Gateway’. Height, percent flowering, and flower number was also recorded. Gemini Granular is 0.40% Prodiamine 0.25% Isoxaben. Eupatorium purpureum ‘Gateway’ liners were transplanted from 50-cell trays into an aged/screened pine bark based medium in 11.4 L containers on June 28, 2024. Medium was amended with sphagnum peat, perlite, a starter dose of 12-6-6 and 15-9-12 controlled release fertilizers. Containers received drip irrigation. Herbicide was applied twice during the season at 6-week intervals with a hand shaker (July 8 and August 20, 2024). Herbicide rates were control (0 lbs./A), 1x (200 lbs./A), 2x (400 lbs./A), or 4x (800 lbs./A). Experimental design was completely random with 10 replications. Data were analyzed in SAS version 9.4 (SAS Institute, Inc., Cary, NC) using PROC GLIMMIX with mean separation using Tukey’s HSD at α = 0.05. Plants exhibited no phytotoxicity, regardless of herbicide rate. Plants receiving Gemini Granular at 200 lbs./A had greater heights compared to the heights of control plants or plants receiving the 2x or 4x herbicide rate. Flower numbers were only reduced for plants receiving the 2x herbicide rate. Flowering percentages ranged from 16.6% for control plants to 5.1% for plants receiving the 2x herbicide rate. In general, weed pressure was low, and there were no differences between treatments for weed number. Gemini Granular applied at 200 lbs./A, the 1x rate, provided effective and safe weed control for Eupatorium purpureum ‘Gateway’.
Speakers
PK

Patricia Knight

Mississippi State University
Co-authors
HW

Haley Williams

Mississippi State University
JR

Jenny Ryals

Mississippi State University
PB

Patrick Broussard

Mississippi State University
NA
SL

Scott Langlois

Miss State University
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB

12:15pm CDT

WCPM 2 - How Low Can You Go? Reducing Variable-Rate Spray Technology Default Rates
Friday August 1, 2025 12:15pm - 1:00pm CDT
Variable-rate spray technology allows nursery producers to make applications tailored to crops, reducing pesticide application volume and off-target losses. The objective of this study was to evaluate sub-default rates (default=0.1-0.15 L⸱m-3) for the Smart-Apply intelligent spray control system by comparing two intelligent modes with the conventional, constant-rate. We assessed control of powdery mildew (Erysiphe pulchra and Phyllactinia guttata) on ‘Cherokee Princess’ dogwoods (Cornus florida) in a five-row block. A Jacto A400/850 air-blast sprayer with the Smart-Apply system was used to compare: variable-rate: 0.05 L⸱m-3 rate in either pulse-on or pulse-off mode and a reduced, i.e.,
Speakers
KM

Karl McKim

The University of Tennessee
Co-authors
AF

Amy Fulcher

University of Tennessee
CY

Cory Yurisic

University of Tennessee
NA
EE

Erica Eady

University of Tennessee
NA
GP

Grace Pietsch

University of Tennessee
NA
HZ

Heping Zhu

USDA-ARS
NA
LN

Lloyd Nackley

oregon state university
Lloyd Nackley is a plant ecologist who applies a systems approach to improve nursery and greenhouse management. Nackley's research program at Oregon State University focuses on addressing four challenges facing nursery and greenhouse production in Oregon: irrigation application, pest... Read More →
SX

Sun Xiaocun

The University of Tennessee
NA
WW

Wesley Wright

University of Tennessee
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Weed Control and Pest Management 1

12:15pm CDT

WCPM 2 - Molecular Insights into the Developmental Regulation of Crapemyrtle Bark Scale (Acanthococcus lagerstroemiae)
Friday August 1, 2025 12:15pm - 1:00pm CDT
Crapemyrtle bark scale (CMBS; Acanthococcus lagerstroemiae) is an invasive phloem-feeding insect that diminishes both the aesthetic appeal and economic value of crapemyrtle (Lagerstroemia sp.), the most widely planted flowering tree in the United States. Conventional control methods, including pruning, scrubbing infested bark, and applying systemic or contact insecticides, offer limited effectiveness due to the insect’s waxy covering, cryptic life stages, and prolific reproduction. Additionally, chemical treatments raise concerns about non-target effects and environmental contamination. To facilitate the development of targeted and sustainable control strategies, we are elucidating the molecular mechanisms governing CMBS development. We have assembled a high-quality reference genome and conducted bulk RNA sequencing across multiple life stages. Building on these resources, we now aim to apply single-cell RNA sequencing (scRNA-seq) to resolve cell-type-specific gene expression patterns throughout development. This high-resolution analysis will uncover key regulatory pathways, informing the identification of novel molecular targets for environmentally responsible CMBS management. We will report on the results of these investigations at the meeting.
Speakers Co-authors
BW

Bin Wu

Texas A
NA
HK

Hazen Keinath

Texas A
NA
HD

Hui Duan

USDA-ARS-USNA-FNPRU
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Weed Control and Pest Management 1
  • Subject Weed Control and Pest Management
  • Funding Source Floral and Nursery Plants Research Unit, U.S. National Arboretum, U.S. Department of Agriculture-Agricultural Research Service

12:15pm CDT

WCPM 2 - Rate and Application Volume Effects on the Efficacy of Glyphosate Alternative Herbicides
Friday August 1, 2025 12:15pm - 1:00pm CDT
Commercial pest control companies as well as homeowners are currently searching for alternatives to the herbicide glyphosate due to negative press and changing consumer preferences. These non-selective alternatives are almost exclusively contact-action herbicides (no systemic activity) and the most common options (diquat, pelargonic acid, acetic acid, plant oils, etc.) have been shown to be effective on annual weeds when label directions are followed. However, labels on individual products often recommend a wide range of application rates (concentrations of the herbicide) and application volumes depending upon weed size, creating confusion for users in terms of what rate and/or application volume is needed for optimal control while keeping costs low. The objective of this research was to evaluate four glyphosate alternatives (acetic acid, caprylic capric acid, diquat, and pelargonic acid) at both high and low labeled application rates and at three different application volumes including 50, 100, and 200 gallons per acre (1.2, 2.3, and 4.6 gallons per 1,000 ft2) to determine how efficacy differed based on these different treatment variables. Two weed species including yellow nutsedge (Cyperus esculentus) and garden spurge (Euphorbia hirta) were used for the evaluation to observe differences between an annual broadleaf (spurge) and a perennial sedge species (yellow nutsedge). Plants were allowed to grow for approximately 6 weeks, at which time herbicides were applied. Data collected included visual control ratings at 1, 2, and 4 weeks after treatment (WAT) and shoot weights collected at 5 WAT. Results differed by weed species and herbicide and on different evaluation dates. For yellow nutsedge, application volume was the most common significant main effect with similar control being achieved at both high and low label rates when the higher application volumes were used. For garden spurge, application volume was mostly insignificant with the high rate providing more consistent control regardless of application volume. Results indicate that for larger or perennial weed species, higher application volumes are more critical to success, given that adequate and label rates are used, as they were needed to achieve more thorough coverage to contact more shoot biomass. In contrast, for the smaller prostrate growing garden spurge, rate was most important as thorough coverage was achieved at the lowest application volumes. This research begins to help us better understand these alternative herbicides and develop recommendations for the most effective and efficient use of these products in Florida landscapes.
Speakers
SC

S. Christopher Marble

University of Florida/IFAS
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Weed Control and Pest Management 1

12:15pm CDT

WCPM 2 - The Antifeedant Activity of Industrial Hemp (Cannabis Sativa) Extracts Against Manduca Sexta Under Laboratory Conditions
Friday August 1, 2025 12:15pm - 1:00pm CDT
Tobacco hornworms (Manduca sexta) are among very destructive insects that feed mainly on plants in Solanaceae plants. The larva stage is the most destructive, feeding on leaves and fruits, and as few as one to three insects can cause 100% loss. Damage caused by hornworms to the stem creates an environment for pathogens to grow, which ultimately kills the entire plant. Synthetic pesticides have been most effective in controlling insect pests but are harmful to the environment and humans. Plant-based biopesticides that are biodegradable, affordable, and eco-friendly may be a sustainable option to mitigate the problem. Several plants have been used as insecticides and have successfully controlled several insects. Due to the increasing demand for safe and biodegradable insecticides, there is a growing interest in sustainable pest control measures, and therefore there is a need to increase the number of plant sources to reduce the gap between demand and supply. Industrial hemp is among potential plants with phytocompounds that can be used as an anti-feeding agent against insects. However, little is known about the ability of the hemp phytocompound as an anti-feeding agent against tobacco hornworms. This study aimed to determine the antifeeding properties of industrial hemp extract against tobacco hornworms in vitro. The ethanolic hemp extracts were prepared at 50, 25, 12.5, 6.25, 3.125, and 1.56 mg/ml. Extracts were incorporated into the artificial diet, fourth instar larvae were introduced into the diet and raised in the growth chamber at 27±2°C, 60±10% RH and photoperiod 12:12 hour light/dark for four days. The relative growth rate differed significantly between concentrations. The growth rate was -0.05 g/g/day at 50 mg/ml, indicating insect body shrinkage, and 0.11g/g/day at 25 mg/ml, indicating slow growth compared to the negative control, which increased by 0.19g/g/day. The highest concentration (50mg/ml) inhibited larvae growth by 100%, similar to Malathion, which was used as a positive control in the study, 25mg/ml inhibited growth by 71%, 12.5mg/ml by 59%, 6.25mg/ml by 51%. Furthermore, an antifeedant index of 68% at 50mg/ml and 62% at 25mg/ml was reported. The findings indicate that industrial hemp extract is a strong antifeedant against tobacco hornworms and, therefore, a strong source of insecticide to control the insect, and thus, a potential contribution to sustainable agriculture.
Speakers
GK

Getrude Kanyairita

Tuskegee University
Co-authors
DM

Desmond Mortley

Tuskegee University
NA
WC

Willard Collier

Tuskegee University
NA
Friday August 1, 2025 12:15pm - 1:00pm CDT
Empire AB
  Poster, Weed Control and Pest Management 1
  • Subject Weed Control and Pest Management
  • Funding Source Contribution of the George Washington Carver Agricultural Experiment Station and USDA/NIFA Evans Allen Program (Grant no. ALX-FVC18)"

1:00pm CDT

CITR 2 - Analyzing the evolutionary relationships and patterns among citrus genotypes to their tolerance to HLB
Friday August 1, 2025 1:00pm - 1:45pm CDT
Citrus production is challenged by multiple biotic and abiotic stresses, with Huanglongbing (HLB) disease being the most damaging to global citrus production. Long-term field evaluations have revealed that certain citrus relatives have varying HLB tolerance. This study investigates the genetic relationships among citrus genotypes with differing HLB tolerance levels to inform future breeding strategies for citrus improvement. The genome sequencing data from 39 citrus species and hybrids were analyzed: 23 tolerant, 8 moderately tolerant, and 8 susceptible to HLB. Variant calling identified 445,222 high-quality SNPs, with chromosome 5 showing the highest contribution of unique variants. Polymorphic information content (PIC) ranged from 0.27 to 0.38, with the highest SNP frequency observed at a PIC value of 0.27 (21,500 loci). The mean heterozygosity across genotypes was 0.136, with Citrus aurantium showing the highest and Citrus polyandra the lowest heterozygosity. Phylogenetic analysis grouped the genotypes into six distinct clusters. Admixture analysis (K=5) was performed to explore ancestral genetic components, while PCA accounted for 62.76% of total genetic variation. These findings provide critical insights for developing HLB-tolerant citrus hybrids and highlight the underlying genetic diversity present in citrus germplasm.
Speakers
MD

Manjul Dutt

University of Florida
Co-authors
JK

Jaideep K Deol

University of Florida
NA
MR

Marcio Resende

University of Florida
NA
SR

Sheetal Ramekar

University of Florida
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Citrus Crops 2

1:00pm CDT

CITR 2 - Comprehensive Study on the Grapefruit and Sweet Orange Fruit Development in Huanglongbing (HLB) Endemic Citrus Orchards
Friday August 1, 2025 1:00pm - 1:45pm CDT
The US third largest citrus industry located in Lower Rio Grande Valley (LRGV), South Texas is renowned for producing premium quality fresh fruit. However, the fresh-fruit industry dynamics have changed significantly as devastating Huanglongbing (HLB) disease become endemic in LRGV citrus groves. The primary reasons that affected the Texas citrus industry are unhealthy crop load, and unnatural and excessive fruit drops in HLB affected trees. Therefore, this study investigates HLB effects on citrus fruit development in the region's subtropical semi-arid climate, focusing on the period from bloom to harvest. Research was conducted on mature 'Rio Red' grapefruit and 'Marrs' sweet oranges grafted onto Sour orange rootstock, all exposed to HLB for over a decade. In both citruses multilocation trial and replicated number of trees were pre-screened for blotchy mottled symptoms on foliage and presence of presumptive causal bacterium of HLB, Candidatus Liberibacter asiaticus (CLas) using qPCR assay. The CLas ve trees were further evaluated for sectorial presence of HLB symptoms in 10 branches and divided into two sub-categories of HLB affected symptomatic (Symp) and apparently healthy, asymptomatic (Asymp) branches. In year-2024, monthly fruitlet retention analysis from April to July reveled significantly higher fruitlets drop in ‘Symp’ branches compared to ‘Asymp’ ones across both citrus varieties. In late-May, the ‘Symp’ grapefruit branches showed 28.5-35.3% drop, while sweet orange experienced 23.3-36.3% losses. While ‘Asymp’ branches had significantly lesser fruit drop ranged in 23.1-27.5% in grapefruit and 22.7-29.4% in sweet orange. Although late-May fruit drop is a natural phenomenon in citrus trees, the excessive fruit drop under HLB disease pressure highlights the negative effect of HLB in fruit retention and possibly in fruit size expansion. Ongoing research continues to identify vulnerable stages of fruit growth and expansion under HLB stress, aiming to develop targeted treatments for maintaining high-quality fruit production under HLB prevalence in LRGV citrus groves with region’s unique environmental and edaphic conditions.
Speakers
JL

Julianna Leal

Texas A
NA
Co-authors
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Citrus Crops 2
  • Subject Citrus Crops
  • Funding Source Texas Citrus Producers Board

1:00pm CDT

CITR 2 - Efficacy of Brassinosteroid in Enhancing Citrus sinensis Health and Performance under HLB Endemic Conditions
Friday August 1, 2025 1:00pm - 1:45pm CDT
Brassinosteroids (Brs), a class of plant steroidal hormones, protect various crop species, including maize, wheat, strawberries, and grapes, from biotic and abiotic stress. However, their impact on citrus remains unexplored. The Florida citrus industry faces a significant threat from Huanglongbing (HLB), a devastating disease that deteriorates tree health and affects overall yield. Given the efficacy of Brs in enhancing stress tolerance in other crops, their potential role in citrus resilience needs an investigation. To address this knowledge gap, we examined the plant's performance after foliar application of Brs at different intervals. Three-year-old Valencia sweet oranges grafted onto UF-R5 rootstock were grown in pots and treated with Brs at three intervals: every two weeks, once a month, and every other month. Key parameters, including shoot and root biomass, canopy volume, scion and rootstock diameters, specific leaf area, total phenolic content, and flavonoid content were assessed. After one year, there was a significant increase in flavonoid content in Brs-treated plants, regardless of application frequency. There was a positive trend on the response to the treatment of the other parameters studied, althoughnot statistically significant by the first year of treatment. This finding suggests that Brs may quickly enhance secondary metabolite production in citrus, but their impact on overall growth may require longer time of exposure to the treatment. Further investigation will elucidate this.
Speakers
DA

Divya Aryal

SWFREC - University of Fl
Co-authors
FA

Fernando Alferez

SWFREC - University of Florida/IFAS
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Citrus Crops 2
  • Subject Citrus Crops
  • Funding Source Citrus Research and Development Foundation (CRDF)

1:00pm CDT

CITR 2 - Evaluation of Four White-Pulp Finger Lime Selections Developed at the University of Florida
Friday August 1, 2025 1:00pm - 1:45pm CDT
Citrus australasica, commonly referred to as finger lime, is an Australian citrus species distinguished by its elongated, cylindrical fruits and numerous small, spherical juice vesicles that resemble caviar. It is highly valued in the culinary industry for its unique appearance, diverse color variations, and distinctive flavor. Finger lime is tolerant to Huanglongbing (HLB), a devastating citrus disease, making it a promising source of resistance genes for developing HLB-tolerant rootstocks and scions. In this study, we conducted a comparative analysis of four of the best University of Florida-developed white pulp finger lime selections - KB1, KB29, KB37, and KB48. These 7-year-old accessions, growing at the University of Florida’s Tropical Research and Education Center campus, have excellent fruit size, robust tree health, and remain HLB negative under endemic HLB conditions. The fruit quality assessment (fruit weight, edible rate, fruit length, diameter, and pearl diameter) revealed that the KB37 selection was significantly superior, followed by the other three finger lime selections, with the lowest values observed in the control, DPI-50-36. Finger lime juice analysis indicated that TSS (total soluble solids) and acid content were almost similar across the four fruit lines, and this was significantly lower than the DPI-50-36 control. The phenolic activity was not statistically different between the selections, while the highest flavonoid activity was observed in KB29, followed by KB1 and KB37. The volatile profiles obtained from the four-finger lime selections were also evaluated. Overall, morphological and physicochemical parameters identified KB37 as the best-performing selection amongst the 4 evaluated. This analysis underscores the nutritional profile of these novel white-pulp finger limes, positioning them as a strong candidate for commercial cultivation for the specialty citrus industry.
Speakers
SR

Sheetal Ramekar

University of Florida
NA
Co-authors
JH

Jonathan H Crane

University of Florida, TREC
MD

Manjul Dutt

University of Florida
NK

Nabil Killiny

University of Florida
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Citrus Crops 2

1:00pm CDT

CITR 2 - Exploring HLB Tolerance in Citrus: A Genomic Evaluation of Early-Season Sweet Oranges
Friday August 1, 2025 1:00pm - 1:45pm CDT
Huanglongbing (HLB), or citrus greening disease, caused by the phloem-limited bacterium Candidatus Liberibacter asiaticus (CaLas), poses a significant threat to citrus production worldwide. Identifying and developing HLB-tolerant cultivars has become necessary to mitigate its devastating impact. In Lake County, Florida, several Hamlin-type citrus trees, that’s are generally susceptible to HLB exhibited significant tolerance to the disease. The CaLas bacterial titer for most of the surviving trees was comparable to that of the susceptible control trees, and the qPCR Ct values ranged from 28 to 37. The visually tolerant citrus trees had increased chlorophyll and carotenoid content compared to the susceptible control trees. Juice quality assessments indicated a brix-to-acid ratio of 15-25, with other juice parameters meeting current U.S. Grade A standards. Interestingly, despite being labeled as Hamlin cultivars, several of these trees displayed variations in seed count, some aligning more with Parson Brown cultivars. To investigate their genetic origin, whole-genome DNA sequencing was performed on these trees, as well as on several Hamlin and Parson Brown clones. Genomic analysis revealed that the escape trees were genetically diverse; most were either likely Parson Brown types or Hamlin clones. Among them, two promising lines, R20-T30 (Parson Brown-type) and R21-T36 (Hamlin-type) were identified as superior and included in the 2024 Citrus Research and Field Trials (CRAFT) program. Both cultivars have demonstrated excellent yield and juice quality. Hamlin types, such as Hamlin 1-4-1 and Hamlin R21-T36, and Parson Brown types, such as Parson Brown F-56-2 and Parson Brown R20-T30, also displayed differences at the genomic level. Whole genome sequencing of the root DNA from these escape trees identified nucellar or zygotic Swingle as the predominant rootstock among escape trees with others similar to Carrizo and Poncirus trifoliata rootstocks. These findings underscore the role of genetic analysis in identifying HLB-tolerant cultivars, establishing a foundation for targeted breeding strategies aimed at improving citrus resilience to HLB.
Speakers
SR

Sheetal Ramekar

University of Florida
NA
Co-authors
GE

Gary England

University of Florida
NA
JK

Jaideep K Deol

University of Florida
NA
MD

Manjul Dutt

University of Florida
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Citrus Crops 2

1:00pm CDT

NUTS - Characterizing The Winter Chilling Requirement of ‘Golden Hills’ Pistachio in Southern New Mexico
Friday August 1, 2025 1:00pm - 1:45pm CDT
In the US, pistachio (Pistacia vera L.) is grown mainly in California, but it is a promising crop for some areas of the arid American Southwest, such as the southern parts of Arizona and New Mexico. The Dynamic Model used for calculating winter chill portions has yielded useful relationships in California between dormant-season temperatures and bud break timing for pistachio. Our objective was to characterize the relationship between bud break timing and winter chill accumulation in southern New Mexico, where, compared to California, there are much larger diurnal temperature fluctuations in winter. This experiment was designed to measure the effects of winter chill accumulation in immature pistachio over the course of one dormant season in Mesquite, New Mexico. The orchard used for the study was planted in 2021; the cultivar was Golden Hills budded onto seedling ‘UCB-1’ rootstocks. Dormant shoots were sampled every two weeks from 52 trees in the orchard from 3 January through 14 March 2025. On each sampling date one 25-30 cm shoot was collected from each tree. Samples were placed in containers with 15 cm of tap water in a growth chamber (Conviron CMP 3244) modified with LED lights (Heliospetra Elixia). The growth chamber light cycle was set to 14 hours on (“day”) and 10 hours off (“night”). The growth chamber air temperature was set to 24°C during the day and 19°C at night and. The samples were arranged in the chamber in a completely randomized block design with six blocks (containers). The average duration from dormant sampling until bud break decreased as sampling dates progressed from early January to mid-March. The time to bud break in the growth chamber for the six samples averaged 37 days in early January. It had dropped to 30 days by late January, and 17 days by the end of February.
Speakers
CC

Chris Chavez

New Mexico State University
NA
Co-authors
CV

Ciro Velasco-Cruz

New Mexico State University
NA
RH

Richard Heerema

New Mexico State University
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Temperate Tree Nut Crops

1:00pm CDT

NUTS - ‘Ga. 03-6-72’, A Scab Resistant Pecan Cultivar with Excellent Kernel Quality for the Southeastern U.S.
Friday August 1, 2025 1:00pm - 1:45pm CDT
‘Ga. 03-6-72’ is a new pecan (Carya illinoinensis) that was released by the University of Georgia (UGA) College of Agriculture and Environmental Sciences for use in commercial orchards in the southeastern U.S. ‘Ga. 03-6-72’ produces large pecans (9.3 g) with consistently high percentage kernel (59.6%). ‘Ga. 03-6-72’ kernels shell out into complete halves with a light golden color and harvest date is early October in southern Georgia. Yields of ‘Ga. 03-6-72’ have been good and similar other commercial cultivars such as ‘Sumner’, ‘Huffman’, and ‘Tom’. ‘Ga. 03-6-72’ has good levels of resistance to pecan scab (Venturia effusa) with little disease seen in orchards receiving moderate levels of fungicide protection and moderate levels of infection in unsprayed orchards. ‘Ga. 03-6-72’ is recommended for trial in commercial orchards in the southeastern U.S. which will receive fungicide applications.
Speakers
PC

Patrick Conner

Professor, University of Georgia
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TCHG - Design and Implementation of an Integrated Case-Study Based Course in Plant Diagnostics
Friday August 1, 2025 1:00pm - 1:45pm CDT
Plant production is challenged by a variety of stressors that limit system health and economic outcomes. Efficient integrated crop management practices have the potential to reduce the impact of these challenges. Application of effective plant protection strategies requires knowledge of the identity of the stressor. A case-study based course was developed providing students with training and experience in the process of plant diagnostics. The goal for the course is to integrate student knowledge of plant biology, soil science, insect pest management, and plant pathology to allow for effective identification of plant health challenges under conditions they will practically experience in a professional setting. Students are challenged to engage in the process of plant diagnostics and management plan development through case studies based in diverse plant production systems. Students additionally gain experience in communicating their results to non-academic audiences. Course participants indicate increased confidence in identification of plant health challenges and communication, as well as increased skill in problem solving in plant systems.
Speakers
RS

Ronald Stephenson

Plant Sciences Hall
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TCHG - Live Crop Library: An Innovative Tool for Education and Outreach
Friday August 1, 2025 1:00pm - 1:45pm CDT
The growing complexity and challenges faced by the US Agriculture industry demands a new generation of highly skilled agricultural professionals equipped with both technical proficiency and practical knowledge. There is also a pressing need to recruit talented students to pursue a degree in plant agriculture and provide quality, hands-on education for those students. Studies indicate that experiential learning activities are a great tool to encourage young people begin careers in agriculture. We established a live crop library on the university farm consisting of agronomic and horticultural crops of Texas. This includes a wide range of vegetable, fruit, herb, grain, and fiber crops. Tropical fruit crops were installed in ground-beds within a greenhouse. This crop library is envisioned as a place for experiential learning, scholarly research, and agricultural outreach activities. The crop library has become a hub for experiential learning activities for both undergraduate- and graduate-level courses in multiple disciplines including plant science, agribusiness, and animal science. Outreach activities geared toward both youth and adults, such as field days, are also being enhanced through the crop library. Quantitative and qualitative data collecting methods are utilized to evaluate the effectiveness of the crop library in enhancing participant’s learning outcomes. Overall, this project provides a stimulus for high school and college students to enhance their knowledge of plant agriculture in Texas. Adult learners gain understanding of new ways to income or assist others in improving their agricultural income. The findings will continue to contribute to ongoing efforts to improve learning outcomes in multiple educational settings.
Speakers
TP

Tim Pannkuk

Associate Professor & Graduate Coordinator, Sam Houston State University
Co-authors
SN

Shyam Nair

Sam Houston State University
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Teaching Methods

1:00pm CDT

TCHG - Perceptions of Topic Selection Preference for Semester Projects in a Plant Propagation Course
Friday August 1, 2025 1:00pm - 1:45pm CDT
Pedagogy strategies that include students in the course curriculum can increase student engagement and success. One student-based pedagogy involvement example is involving students in the assignment implementation. This method was adapted after feedback from student evaluations indicated that the topic selection method for semester-long course projects was challenging in the HORT 260 Plant Propagation course at Colorado State University. The course curriculum includes two projects that focus on students writing propagation protocols on specific plant species of their choice. The selection process became a stressor for assignment completion due to the paradox of choice. With this in mind, we revised the assignment, testing topic selection methods for the Spring 2024 and 2025 semesters. The study trialed three topic selection methods (treatments): find their plant independently, choose from a list, or be assigned a plant. Each student experienced two of the three treatments, one treatment type per assignment. We obtained student feedback through voluntary pre- and post-surveys to assess their experiences and satisfaction. The pre-survey was administered after they selected their topic plants, and the post-survey was disseminated after the submissions of the projects’ final drafts. In Spring 2024, 28 students participated in the pre- and 24 in the post-survey. Of the 18 students who participated in both surveys, the post-survey resulted in a preference for selecting a plant from a list rising 11% and preferences for methods where students independently found their topic plant or were assigned a plant both dropped 5%. When students were asked why they chose a plant as their topic, 61% chose based on familiarity, 25% on unfamiliarity and desire to learn more, 11% for “other reasons”, and 3% selected plants they thought would be the easiest to write about. In the post-survey, students noted in the open response that they felt that plants selected from a list were easier to find information needed to complete the assignment and 39% of respondents suggested that picking from a list should be the method in the future. The other most reoccurring comment was that 32% of respondents said interest in the topic plant made the project easier to complete. Based on our observations for Spring 2024 and initial data from 2025, providing a list to select from is the most fruitful way to give students the opportunity for choice while minimizing the possibility of topic choice paralysis.
Speakers
ML

Makenzie Lee

Colorado State University
Co-authors
AL

Amy Lentz

Colorado State University
NA
CT

Chad T. Miller

Colorado State University
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TCHG - Publish What You Present at the Annual Meeting
Friday August 1, 2025 1:00pm - 1:45pm CDT
The annual meeting of the American Society for Horticulture Science is over, and you presented great data. Now what? Publish your data with ASHS. We have three highly respected journals: The Journal of the American Society for Horticulture Science (JASHS), HortScience, and HortTechnolgy. Many people who attend the meeting look forward to seeing the paper related to what was presented at the meeting. There are many reasons to publish with ASHS including this is our national society and these journals are the outreach for our society; we have open access publications; the publishing costs are reasonable compared to other open access platforms; the society has improved the submission and review process; reviews are completed in a timely manner; and the journals continue to have increased impact factors. In fact, JASHS impact factor had increased from 0.95 in 2019 to 1.53; HortScience has increased from 1.10 to 1.87; and HortTecnology has increased from 0.67 to 1.39. This poster will outline the process of presenting at the meeting to publishing the results. It also will compare costs with other open access publications and show the improved submission and review process.
Speakers
KM

Kimberly Moore

University of Florida
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

TCHG - Undergraduate Horticulture Program Evaluation and Alignment of Learning Outcomes Through Curriculum Mapping
Friday August 1, 2025 1:00pm - 1:45pm CDT
Curriculum mapping is a valuable tool for visualizing how course content and learning activities align with program-level learning outcomes. In higher education, this method enables faculty to design cohesive curricula that foster integrated learning experiences across a student's academic career. While individual course outcomes are routinely assessed, program-level mapping offers a broader view that supports continuous improvement and ensures graduates possess the skills and knowledge required for professional success. At a midwestern land-grant university, the undergraduate Horticulture major program participated in a university-wide pilot project to map and evaluate its curriculum during the 2024–2025 academic year. The initiative was supported by several institutional learning units and a dedicated team of assessment and curriculum specialists. Faculty collaborated to analyze course goals, identify where embedded learning outcomes are being met, and develop a program-wide curriculum map and accompanying narrative. The mapping process focused on identifying where learning outcomes are introduced, reinforced, and mastered throughout the curriculum. Special attention was given to senior-level capstone courses, where embedded outcomes are being assessed through targeted assignments and artifacts. Analysis of specific courses across the map and across academic years will highlight gaps, redundancies, and alignment with employer expectations for graduates. The resulting curriculum map and supporting documents serve as dynamic tools for ongoing evaluation. They guide future assessment efforts, support accreditation needs, and inform curriculum planning at the university, college, and departmental levels. The methodology used to align course- and program-level outcomes with institutional, college and departmental goals will be shown, along with the products of the process, and future plans and strategies for further data collection and analysis will be shared.
Speakers
avatar for Kathryn Orvis

Kathryn Orvis

Professor, Purdue Univ
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VGBR - Evaluating Watermelon (Citrullus spp.) F2 Hybrid Populations for Phytophthora capsici Resistance.
Friday August 1, 2025 1:00pm - 1:45pm CDT
Watermelon, Citrullus lanatus, is an important vegetable crop in the United States of which the annual watermelon crop value exceeded $534 million in 2021. In recent years, production has been disrupted by the rapid spread of a serious plant pathogen, Phytophthora capsici. The closest ancestors of today’s domesticated watermelon are thought to be Citrullus mucosospermus and Citrullus amarus, several accessions of which have known resistance to Phytophthora capsici fruit rot, but are untested in regards to stem and root rot. Popular commercially available cultivars, such as ‘All Sweet’, ‘Laelanau’, ‘Penghu’, and ‘Sunshade’, as well as Texas A
Speakers
RE

Ryan Elms

Texas A
Co-authors
JJ

John Jifon

Texas A
NA
KC

Kevin Crosby

Texas A
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VGBR - Introducing Resistance to Fusarium Wilt Race 3 of Tomato Through Background Genome Selection
Friday August 1, 2025 1:00pm - 1:45pm CDT
Fusarium wilt, caused by the fungal pathogen Fusarium oxysporum f.sp. lycopersici (Fol), threatens tomato crops globally. Fol causes substantial yield losses in susceptible plants and is persistent once established in fields, making gene-based resistance a high-priority for growers. Historically, Fol strains isolated in the Midwest United States have been predominantly race 1, and I-based resistance has been widely deployed and effective. However, there have been increasing numbers of samples sent to diagnostic clinics in the Midwest with Fusarium wilt symptoms, suggesting a potential shift in Fol race prevalence and the need to deploy additional resistances. The aim of our project is to introgress the I3 resistance gene, effective against race 3 Fol, into Midwest-suited processing tomato lines. I3 was originally identified in Solanum pennellii, a wild relative of cultivated tomato (Solanum lycopersicum), and a minimal introgression which reduced linkage drag was developed and introduced into Fresh-Market tomatoes by the University of Florida. We developed backcross families with the I3 gene using the Florida minimal introgression and recurrent parents from the Ohio processing tomato breeding program. Lines were selected for advancement based on I3 genotyping, background genome selection using unlinked single nucleotide polymorphisms (SNPs), and phenotyping of relevant yield and quality characteristics. Our analysis found no significant differences in yield and quality traits between the backcross selections and their recurrent parents, indicating the rapid conversion of parents through this strategy. We are currently combining the I3 and I2 resistance genes through further crossing and will ultimately develop high-performing processing tomato lines suited to Midwest growing conditions that are resistant to all three Fol races.
Speakers
AK

Adelle Knight

The Ohio State University
Co-authors
AT

Anna Testen

USDA-ARS Application Technology Research Unit
NA
DF

David Francis

The Ohio State University
NA
JC

Jessica Chitwood-Brown

University of Florida
JC

Jin Cho

The Ohio State University
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Breeding

1:00pm CDT

VGBR - Morphological Characterization of UGA-CAPSI-CORE (Capsicum spp.) reveals extensive diversity for trait improvement
Friday August 1, 2025 1:00pm - 1:45pm CDT
Understanding germplasm diversity is essential in pepper (Capsicum annuum L.) breeding to enhance disease resistance and fruit quality. This study evaluated morpho-phenological characteristics of UGA-CAPSI-CORE, a collection of 352 globally sourced germplasm classified into six varietal groups (VGs): banana, bell, Italian frier, mini bell, mini sweet, and specialty peppers evaluated in two replications during summer 2024. A total of 12 traits, including eight quantitative (e.g., germination, plant vigor, plant height) and four qualitative traits (e.g., growth habit, fruit position, fruit color), were assessed. Significant phenotypic variation was observed across varietal groups. Germination rates averaged 64%, with mini sweet peppers exhibiting highest germination (71.63%) and least in banana peppers (57.30%). Flowering time slightly varied (87–103 days), with banana peppers flowered late (89.3 days) than Italian frier (88.5 days). Specialty peppers were longer (41.49 cm) while bell types shorter (34.22 cm). Banana peppers and bell types exhibited taller (17.55 cm) and shorter (14.98 cm) stems, respectively. Lodging susceptibility was highest in Italian frier (29.12%) and lowest in mini bell (19.78%). Days to maturity was consistent, with mini sweet accessions requiring slightly longer time (142.51 days) than Banana peppers (141.34 days). Fruit morphology varied significantly, with bell peppers producing the highest lobe count (3.38) and banana peppers having single-lobed fruits. ANOVA confirmed significant variation (p < 0.001) in key traits, and cluster analysis identified six distinct clusters. This study elucidates the genetic diversity within the evaluated germplasm, providing valuable insights for breeding program to focus on enhancing productivity, adaptability, and fruit quality.
Speakers
AN

Amol Nankar

University of Georgia (UGA)
Co-authors
DJ

Deepa Jaganathan

University of Georgia
Post Doc at University of Georgia with over 15 years of experience in molecular breeding and gene editing in crops. Research expertise spans drought tolerance in chickpea, TYLCV resistance in tomato, salinity tolerance in rice, genetic mapping in banana, and currently focused on disease... Read More →
NT

Noel Thomas

University of Georgia
NA
TM

Ted McAvoy

University of Georgia
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VGBR - Screening and Identification of Resistance Sources to Phytophthora in Peppers (Capsicum annum L.) Through Marker-Assisted Selection Using SSR markers
Friday August 1, 2025 1:00pm - 1:45pm CDT
Phytophthora capsici is a devastating pathogen in peppers (Capsicum annuum L.), responsible for root, stem, and fruit rot, often leading to yield losses of 40% to 60% in outbreak conditions. Marker-assisted selection (MAS) offers a precise strategy for accelerating resistance breeding. While high-throughput genotyping approaches such as genome-wide resequencing (WGRS) are increasingly adopted, SSR markers remain a practical and informative tool for initial diversity assessment, especially in large and diverse germplasm collections. In this study, 485 globally sourced pepper accessions including 473 accessions from C. annuum and 12 accessions from seven wild Capsicum species were evaluated using 31 SSR markers previously reported to be linked with Phytophthora resistance. Eighteen markers showing robust and reproducible amplification were selected for full-panel genotyping. To ensure consistent and accurate allele calling, SSR allele binning was performed using TANDEM software. The resulting dataset revealed substantial allelic richness, reflecting the broad genetic diversity of the collection. Gradient PCR was also performed to optimize amplification of the 13 initially non-amplifying markers, resulting in six additional markers now suitable for further screening. Downstream diversity analyses, including PCA, STRUCTURE, and phylogenetic clustering, are currently in progress. Additionally, WGRS is planned for this germplasm panel, and integration of SSR and SNP datasets will allow for a comprehensive assessment of genetic relationships, population structure, and resistance allele distribution. Together, these efforts will support the identification of novel resistance sources and inform strategic breeding for improved Phytophthora resistance in pepper.
Speakers
AN

Amol Nankar

University of Georgia (UGA)
Co-authors
DJ

Deepa Jaganathan

University of Georgia
Post Doc at University of Georgia with over 15 years of experience in molecular breeding and gene editing in crops. Research expertise spans drought tolerance in chickpea, TYLCV resistance in tomato, salinity tolerance in rice, genetic mapping in banana, and currently focused on disease... Read More →
JG

Jessica Gibbs

University of Georgia
NA
MA

Mahnaz Azad

University of Georgia
NA
MT

Miaoying Tian

University of Georgia
NA
NT

Noel Thomas

University of Georgia
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Vegetable Breeding
  • Subject vegetable Breeding
  • Poster # csv
  • Funding Source Georgia Department of Agriculture (GDA) Specialty Crop Block Grant Program (SCBGP) - RGDAG000188920A

1:00pm CDT

VGBR - Seed Multiplication and Phenotypic Assessment of a Globally Sourced Capsicum Collection to Support Pre-Breeding Efforts
Friday August 1, 2025 1:00pm - 1:45pm CDT
Seed increase is an essential step in germplasm management, enabling the effective use of genetic resources in breeding, evaluation, and conservation. This study focused on multiplying seed stocks and assessing phenotypic diversity in a globally sourced collection of 485 Capsicum accessions. The collection comprises accessions belonging different species of Capsicum including C. annuum (473), C. chinense (4), C. baccatum (3), C. chacoense (1), C. flexuosum (1), C. frutescens (1), C. galapagoense (1), and C. rhomboideum (1). Evaluated accessions were obtained from diverse genebanks (domestic and international), and collaborative breeding programs that comprises landraces, heirlooms, breeding lines, and exPVPs. To ensure sufficient seed availability, 352 accessions were grown in a greenhouse during summer 2024 and an additional 133 accessions were grown during winter 2024. Simultaneously, twelve agronomic and morphological traits, six pre-harvest (e.g., germination rate, plant vigor, internode number and length) and six post-harvest (e.g., fruit size, pericarp thickness, yield) were recorded. Significant phenotypic variation was observed across varietal groups. Bell and mini bell peppers showed high germination rates (>65%) and marketable yields, with bell types producing the heaviest fruits (79.8 g) and thickest pericarps (5.2 mm). Banana peppers exhibited the longest fruits (13.8 cm) and high plant vigor, while mini bells were completely resistant to lodging. These findings highlight both successful seed multiplication and the phenotypic richness of the collection, offering a valuable resource for pre-breeding, core collection development, and trait-specific selection.
Speakers
AN

Amol Nankar

University of Georgia (UGA)
Co-authors
BD

Bhabesh Dutta

University of Georgia (UGA)
NA
DJ

Deepa Jaganathan

University of Georgia
Post Doc at University of Georgia with over 15 years of experience in molecular breeding and gene editing in crops. Research expertise spans drought tolerance in chickpea, TYLCV resistance in tomato, salinity tolerance in rice, genetic mapping in banana, and currently focused on disease... Read More →
NT

Noel Thomas

University of Georgia (UGA)
NA
TM

Ted McAvoy

University of Georgia
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 2 - Bacteriophage Trunk Injection Treatment Trial for Pierce’s Disease in ‘Vidal Blanc’ Grapevines
Friday August 1, 2025 1:00pm - 1:45pm CDT
Pierce’s Disease (PD) is a major disease of grapevines in the southeast US caused by the xylem dwelling bacteria Xylella fastidiosa subsp. fastidiosa (Xf). XylPhi-PD is a bacteriophage product recently registered for PD management in grapevines in Georgia. In 2023 and 2024, ‘Vidal blanc’ hybrid winegrape vines with a history of PD were either ‘treated’ at the maximum label rate or ’untreated’ in a commercial vineyard in Lumpkin Co. GA. Disease was rated visually by two raters in September shortly after harvest. A subsample (n=80) was assessed by AmplifyRP XRT to confirm visual assessment. Overall, there was a reduced disease incidence and severity in the treated vines for both years (chi-sq p
Speakers
SL

Sarah Lowder

University of Georgia
Co-authors
CM

Clark MacAllister

University of Georgia
NA
SB

Shane Breeden

University of Georgia
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 2 - Bridging Gaps in Vineyard Nutrient Management through the HiRes Project: A Data-Driven Approach
Friday August 1, 2025 1:00pm - 1:45pm CDT
The High-Resolution (HiRes) Vineyard Nutrient Management Project is developing precision agriculture tools for vineyard nutrition monitoring and refining current guidelines for all grape markets (wine, table, raisin, and juice) in the United States (US). In 2021, an industry survey was conducted, and data was received from commercial grape growers in 25 states. These results provided a nutrient management baseline: growers use annual vine tissue testing and periodic soil testing by manually collecting vine tissues (petioles and leaf blades) and/or soil and sending them to labs for analysis. There is low adoption of precision agriculture methods in farming, and the reasons cited were perceived high costs and technical complexity. In 2025, focus groups were conducted to gather data on how growers prioritize nutrient management, methods used for monitoring, and openness to adopting new vineyard nutrition sensor technologies. Focus groups were conducted across grape-producing states representing eastern and western US regions. These were semi-structured discussions using a uniform questionnaire. Each meeting consisted of 5-10 participants, including vineyard managers, vineyard or winery owners, and other industry stakeholders. Discussions focused on grower decision-making processes, valuation of nutrient management, and strategies for integrating precision agriculture technology. Results show that growers highly value vineyard nutrition for maintaining vine health and crop balance, as found in the 2021 survey. Participants emphasized challenges in field sampling efficiency, consistency, cost, and uncertainty in interpreting results for management plans. Participants had ongoing concerns about input costs and the practical limitations of current equipment for variable-rate applications, particularly for micronutrients. Although growers indicated low use of precision agriculture methods in 2021, focus group results show strong interest in new tools. However, these tools need to be affordable, user-friendly, allow for in-house data, and provide actionable recommendations tailored to specific vine phenology and regional soil variability. A growing interest in regenerative and organic approaches emerged as a significant management theme across regions. These findings are forming the project’s outreach to deliver comprehensive educational resources and user-friendly sensor technologies to enhance grower vineyard nutrient monitoring practices.
Speakers
KS

Karmandeep Sidhu

Graduate Research assistant, Oregon State University
I am a first-year graduate student in Horticulture at Oregon State University, Corvallis
Co-authors
AH

Andrew Harner

Virginia Tech
NA
JV

Justine Vanden Heuvel

NYSAES Cornell University
NA
MF

Matthew Fidelibus

University of California, Davis
NA
NS

Nataliya Shcherbatyuk

Washington State University
NA
PS

Patricia Skinkis

Oregon State University
NA
TB

Terence Bates

Cornell University, NY
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Viticulture and Small Fruits 2
  • Subject Viticulture and Small Fruits
  • Funding Source National Institute of Food and Agriculture’s (NIFA) Specialty Crop Research Initiative Coordinated Agricultural Projects (CAP) grant. Project Award Number: 2020-51181-32159

1:00pm CDT

VSF 2 - Co-regulation of a CBF gene by ICE and FAMA reveals crosstalk between cold acclimation and the stomatal development pathways in grape
Friday August 1, 2025 1:00pm - 1:45pm CDT
Grape (Vitis spp) has four ICE genes encoding proteins with possible functions as transcription factors in an ICE-CBF cold acclimation pathway. Using an Agrobacterium-mediated transactivation system, it was determined that Vitis riparia ICE activates the VrCBF4 promoter after a 4-day incubation period at 22 C when effector and reporter bacteria were at an OD600 of at least 0.25 and 0.5, respectively. All four grape ICE proteins activated VrCBF4 and VrCBF6 promoters. These promoters were used as reporter because both these CBF genes are abundantly expressed in overwintering mature grape buds and therefore thought to be important for freezing tolerance in grape. VrCBF4 was activated most by VrICE2 and VrICE3 whereas VrCBF6 was activated most by VrICE1 and VrICE4. An additional 2 hours of cold treatment increased activation by only the ICE proteins, VrICE2 and VrICE3 that contain a sumoylation site. This supports the notion that low temperature stabilizes VrICE2 and VrICE3 protein by sumoylation. Using a 5’ deletion series of the VrCBF4 promoter it was found that the -281 to -342 bp region contains sequence sufficient for activation by the VrICE proteins or stomatal protein VrFAMA(L). Analyses with a wild type or mutated MYC2g element plus min35S promoter showed that VrICE2, VrICE3 and FAMA(L) activated via this element. The higher activation by combinations of VrICE and VrFAMA(L) suggests that they interact with each other to direct VrCBF4 activation which showed possible overlap between stomatal development and cold acclimation pathways.
Speakers
MR

M Rahman

Alabama State University
Co-authors
AE

Ali Ebadi

University of Guelph
NA
AN

Annette Nassuth

University of Guelph
NA
MS

Mahbuba Siddiqua

University of Guelph
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 2 - Developing a Nutrient Management Strategy for High Tunnel Red Raspberry Production in the Lower Midwest
Friday August 1, 2025 1:00pm - 1:45pm CDT
High tunnel hydroponic systems are increasingly adopted to extend the growing season and improve fruit quality in red raspberry (Rubus idaeus) production. While nutrient management guidelines exist for northern climates, they are often not suited to Lower Midwestern systems especially those using soilless substrates such as coconut coir. In these cooler climates, nitrogen uptake is limited by low root zone temperatures and reduced microbial activity, often resulting in unchecked vegetative growth that shades developing fruit and reduces yield and quality. In contrast, warmer Lower Midwestern high tunnels promote faster nitrogen uptake due to elevated root temperatures and increased plant metabolic rates. While this enhances nutrient absorption, it also encourages overly vigorous vegetative growth when fertility is not carefully managed, limiting light penetration and shifting resources away from fruit development. These challenges highlight the need for region specific nitrogen thresholds that optimize fruit yield without promoting excessive shoot growth. Without region-specific fertility strategies, nutrient applications often exceed crop demand, reducing efficiency and productivity. This project aims to develop optimized nutrient management strategies tailored to soilless raspberry production in Lower Midwestern high tunnels. A completely randomized split-plot experiment was conducted in a high tunnel located in southern Illinois. Three nitrogen fertigation rates (low, medium, high) were assigned to main plots, and five foliar nutrient treatments to split plots. Raspberries were grown in coconut coir, and each of the 15 treatment combinations was replicated four times (n = 60 plots), with five plants per plot and two fruiting canes per plant. Measured variables included shoot length, leaf number, total fruit yield, average berry weight, and foliar nutrient concentrations. These metrics were used to evaluate treatment effects on nutrient efficiency and overall plant performance. Results showed a 222.5% increase in potential fruit yield between the lowest and highest producing plots per 9 × 27-meter-high tunnels. However, this yield gain was accompanied by a 5.9% decrease in average berry size, indicating a trade-off in which higher yields are associated with smaller berries, while lower yields produce larger fruit. This research provides practical, science-based fertility recommendations to growers for improvement of fruit quality and yield, reduced fertilizer waste, and enhanced sustainability and profitability of raspberry production in Lower Midwestern high tunnels.
Speakers
KG

Kayla Grey

Southern Illinois University - Carbondale
Co-authors
AW

Alan Walters

Southern Illinois University
AW

Amanda Weidhuner

Southern Illinois University Carbondale
BT

Bradley Taylor

Southern Illinois University - Carbondale
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Viticulture and Small Fruits 2
  • Subject Viticulture and Small Fruits
  • Funding Source This work was supported by a grant from the SIU Advanced Energy Institute, which gratefully acknowledges funding awarded through the Advanced Energy Resource Board.

1:00pm CDT

VSF 2 - Developing a Pitless, Thornless, and Compact Blackberry using Gene Editing Technologies
Friday August 1, 2025 1:00pm - 1:45pm CDT
Pairwise is pioneering the application of CRISPR technology in food and agriculture. Our company brings together leaders in agriculture, technology, and consumer foods to harness the transformative potential of novel genomics technologies to create innovative new products. We are working to develop new varieties of crops, and to partner with organizations that seek to deliver innovation across the plant-based economy. At Pairwise, we have established robust and reproducible product development pipelines in multiple crops that rapidly propel research from proof-of-concept to product development. We have recovered and characterized edited events in tetraploid blackberry (Rubus subg. Rubus) to improve architecture and agronomic traits. In this presentation, we will share insights and progress on the recovery of edited blackberry plants with desired trait phenotypes.
Speakers
BC

Bruno Casamali

Pairwise Plants
Co-authors
AR

Anabel Rivas

Pairwise Plants
NA
JR

Joel Reiner

Pairwise Plants
PM

Pradeep Marri

Pairwise Plants
TP

Tom Poorten

Pairwise
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 2 - Evaluating Texture and Shelf Life in Muscadine Varieties
Friday August 1, 2025 1:00pm - 1:45pm CDT
Consumer expectations for grapes often center around their visual appeal, taste, and mouthfeel. For instance, the satisfying crunch of a crisp green grape is highly desirable. Similarly, grocery stores maintain strict standards for freshness to ensure marketability. However, these expectations are predominantly based on the vinifera grape, setting a benchmark that muscadines—though beloved in the Southeastern United States—struggle to meet. Muscadine grapes differ significantly from table grapes, leading to challenges in consumer acceptance and retail adoption. Thin-skinned and firm-fleshed berries are selected in our breeding program to make muscadines more palatable to a wider audience and more appealing to retailers, but could potentially reduce shelf life by increasing transpiration rates. Given these concerns, our study examined a range of attributes related to berry texture and their effects on storage ability. Thirty muscadine genotypes were evaluated over a month-long storage period in which attributes such as firmness, skin thickness, and flesh texture were measured. Our findings indicate that berry firmness decreases during storage relative to initial firmness. However, berries ranking firmer at harvest remained firmer after storage compared to softer genotypes. Importantly, our breeding efforts to enhance texture showed minimal impact on storage ability. These results suggest that selecting for vinifera-like texture traits in muscadines can improve consumer and retailer acceptance without significantly compromising shelf life, offering promising implications for expanding muscadine markets nationwide.
Speakers
AM

Anne Marie Gahagan

University of Georgia
Co-authors
AD

Angelos Deltsidis

University of Georgia
PC

Patrick Conner

University of Georgia
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 2 - Evaluating the Performance of Muscadine Grape (Vitis rotundifolia Michx.) Advanced Selections under Central Alabama Conditions
Friday August 1, 2025 1:00pm - 1:45pm CDT
The muscadine grape (Vitis rotundifolia Michx.) is a native fruit crop of the Southeastern U.S., known for its resiliency, high nutritional value, and relatively low input cost in comparison to bunch grapes. Muscadine grape breeding efforts aim to answer production challenges by developing new germplasm with superior traits such as cultivars with large berry size, increased yield, and improved fruit quality attributes. This study aimed to evaluate the overall performance of the University of Arkansas breeding program advanced selections ‘AM-70’, ‘AM-195’, ‘AM-26’ and ‘AM-77’ and the University of Georgia bred selections ‘Ga. 10-1-222’, ‘Ga. 13-4-2’, ‘Ga. 6-1-269’ and ‘Ga. 10-1-294’ in Alabama conditions. Plants of ‘RubyCrisp’ were included for comparison. The experimental vines were planted at the Chilton Research and Extension Center (USDA hardiness zone 8A), Clanton, AL, in 2021. A RCB design with four single plant replications was utilized. Harvesting season spanned from Aug. 9 to Sept. 18, 2024. Fruit of ‘Ga. 10-1-222’, ‘Ga. 13-4-2’, ‘Ga. 6-1-269’, ‘Ga. 10-1-294’, ‘AM-70’, ‘AM-26’and ‘AM-77’ ripened early whereas ‘AM-195’ had a mid-season ripening and ‘RubyCrisp’ matured late in the season. Results suggest ‘Ga. 10-1-294’, was the highest yielding selection, with a total yield of 27.7 kg/vine, compared to the standard cultivar ‘RubyCrisp’, which yielded 24.3 kg/vine. However, no significant difference in total yield among the tested selections was found. The berries of ‘Ga. 6-1-269’ had the largest individual berry weight of 11.9 g, followed by cultivar ‘RubyCrisp’ (11.5 g). ‘AM-195’ berries were the firmest and it was found that the selections differ in flesh firmness. While ‘RubyCrisp’ produced the highest percent berries with wet stem scar (27.6%), ‘AM-26’ had the highest percent berries with dry stem scar (92.9%). ‘AM-70’ berries were the sweetest (17.10 Brix), followed by ‘AM-195’ (15.20 Brix). The TSS:TA ratio varied between 16.7 and 41.0 and was highest for ‘AM-70’. The important fruit productivity and fruit quality parameters evaluated in this study can inform breeders on selection of superior cultivars of muscadine grapes.
Speakers
avatar for Jagjit Singh

Jagjit Singh

Graduate Research Assistant (MS), Auburn University
Co-authors
EV

Edgar Vinson

Auburn University
EC

Elina Coneva

Auburn University
ML

Margaret Leigh Worthington

University of Arkansas
MT

Marlee Trandel Hayse

Auburn University
MR

Melba Ruth Salazar Gutierrez

Auburn University
NA
PC

Patrick Conner

University of Georgia
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Viticulture and Small Fruits 2

1:00pm CDT

VSF 2 - Glycine Betaine Reduces Freeze Damage in ‘Victoria’ Blackberry.
Friday August 1, 2025 1:00pm - 1:45pm CDT
Blackberry (Rubus spp.) growers in the Pacific Northwest (PNW) regularly experience freeze damage, which limits yields and on-farm revenues. Freeze mitigation using wind machines, in-field heaters, and overhead sprinkler irrigation is costly and may not be economical. Sprayable biostimulants, in contrast, are potentially more affordable and readily adaptable as they can be applied using equipment readily available on most farms. However, the efficacy of biostimulants at mitigating freeze damage is largely unknown. The objective of this study was to evaluate the effect of a glycine betaine-based biostimulant on the freezing damage of ‘Victoria’ floral buds. The biostimulant (FRUIT ARMORTM) was applied four days before a freezing event at its highest label rate (4.5 kg/ha) to three consecutive rows in different areas of a commercial farm in Skagit County, Washington. The foliar application was compared to an untreated control. Floricanes from both treatments were collected two days after a second frost and scored for damage by quantifying the presence or absence of damage to primary floral buds from a total of 720 treated and 720 untreated buds. A mixed-effect logistic regression model was used to analyze the data and account for cane and location variability, as well as estimate the expected probability of damage to the floral buds. The foliar application of glycine betaine increased freezing tolerance and reduced the expected probability of damage in the buds by 5.5%. Furthermore, the odds of bud damage in the control were 1.42 higher compared to treated buds. Results indicate that glycine betaine promotes freeze tolerance and may be a cost-effective strategy for blackberry growers to reduce freeze damage. Further research should be done to evaluate other biostimulant active ingredients as well as the optimal timing of biostimulant application and cost-benefits relative to other freeze mitigation practices.
Speakers
PR

Pedro Rojas-Barros

Washington State University
Co-authors
AF

Ashley Fincham

Washington State University
NA
CK

Clark Kogan

Washington State University
NA
LW

Lisa Wasko DeVetter

Washington State University
Lisa Wasko DeVetter joined Washington State University in 2014 and leads the small fruit horticulture program at the Northwestern Washington Research and Extension Center in Mount Vernon, Washington. She has developed a diverse research and extension program with an emphasis on maximizing... Read More →
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
  Poster, Viticulture and Small Fruits 2

1:00pm CDT

VSF 2 - How Do Primocane-Fruiting Blackberries Allocate Nitrogen Fertilizer? First Year of 15Nitrogen Study with ‘Prime-Ark® Traveler’
Friday August 1, 2025 1:00pm - 1:45pm CDT
Research on nitrogen (N) allocation and accumulation in blackberries has primarily focused on floricane-fruiting varieties. However, there is a gap in existing literature on primocane-fruiting blackberry N allocation due to how relatively new primocane-fruiting blackberries are to the commercial industry. The objectives of this study were to investigate how fertilizer N is partitioned among plant parts in ‘Prime-Ark Traveler®’ and quantify the percent N recovery by the plant. In 2023, 15N labeled ammonium-nitrate was applied at a rate of 134.4 kg N/ha to a planting of ‘Prime-Ark Traveler®’ blackberries at the University of Arkansas Fruit Research Station in Clarksville, AR. Plants were fertilized via drip-irrigation over fifteen weeks. In 2023, plant biomass samples were collected in triplicate at four physiological stages: (1) small green floricane fruit, (2) peak-floricane harvest, (3) peak-primocane harvest, and (4) after floricane senescence. This paper focuses on results of leaf, cane, and crown 15N content. Due to senescence, floricanes were not collected at stage 4. Plant parts were separated by cane type before being dried and analyzed to determine mass fertilizer-N recovery (MFNR) and fertilizer recovery efficiency (FRE). MFNR represents the uptake of 15N fertilizer by individual plant organs (g/plant organ) at a given stage. FRE was calculated as a percent of 15N fertilizer in the plant (MFNR) out of total applied (g), with total FRE determined by averaging the FRE of plants at stage 4 and accounting for removed tissues such as fruit and floricanes. Primocane leaf MFNR was highest at stage 3 (1.84 g) and significantly different than stages 1 and 2. Physiological stage did not impact floricane leaf MFNR. Primocane cane MFNR was highest in at stage 3 (0.78 g) and was significantly different than stages 1 and 2. Floricane cane MFNR was highest at stage 1 (0.50 g) and was significantly different than all other stages. No significant differences were observed for MFNR in crowns across physiological stage. The finding that floricane 15N decreased over the season while primocane 15N increased are consistent with current literature on floricane fruiting types of blackberry which indicate that fertilizer applied in-season is generally allocated toward the primocane, however some N uptake in-season by the floricane does occur. Total plant FRE at stage 3 was 21.23%. High soil N levels at the trial site may have diluted uptake of applied 15N. These preliminary results represent the initial year findings of a multi-year trial.
Speakers
AL

Amanda Lay-Walters

University of Arkansas
Co-authors
AM

Amanda Mcwhirt

University of Arkansas
RT

Renee Threlfall

University of Arkansas
NA
RD

Ryan Dickson

University of Arkansas
NA
TR

Trenton Roberts

University of Arkansas
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 2 - Raspberry Resilience: Enhancing Fertilization and Sustainability Practices for Primocane and Floricane Cultivars in the Mid-Atlantic
Friday August 1, 2025 1:00pm - 1:45pm CDT
This poster presents findings from NE-SARE Professional Development Program, which provided critical training in raspberry nutrition management to 87 participants across seven workshops in 2024. The focus was on Floricane and Primocane raspberries grown under high tunnel systems, targeting small-scale and underserved growers in Maryland, Delaware, and Virginia. Participants learned advanced fertilization techniques, including injector use, dose calculations, and liquid versus granular fertilizer applications. Hardwood cuttings valued at $5,000 were distributed for vegetative propagation—transforming waste material into economic assets for 25 farmers. The workshops also introduced split-dose fertilization and nutrient timing, equipping participants to enhance productivity and reduce environmental impact. Demographically, the training reached a diverse audience, with 66.7% of surveyed participants identifying as Black and 44.4% as Black female farmers. Pre-training surveys revealed significant knowledge gaps—88.9% rated their understanding of fertilization topics as 1 out of 5. Post-training responses showed marked improvements in technical skills, confidence, and intent to implement sustainable practices. All participants planned to adopt at least one technique such as split-dose fertilization or injector-based nutrient delivery. The project not only advanced skill development but also inspired proactive change in raspberry cultivation, promoting equity and sustainability in Mid-Atlantic agriculture.
Speakers
ND

Naveen Dixit

University of MD Eastern Shore
NA
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB

1:00pm CDT

VSF 2 - The Novel use of Small Molecules for Mitigating Herbicide Drift in Grapevines
Friday August 1, 2025 1:00pm - 1:45pm CDT
In Texas, and across the country increased use of auxin like herbicides (ALH) have led to a significant rise in instances of off target injury, either through direct spray drift or volatilization. Commonly used in row crop production for control of broadleaf weeds, ALH have the ability to travel several miles under optimal environmental conditions, increasing the chance for off target exposure. Through the increased adoption of ALH resistant row crops, incidents of drift damage have only increased. Currently growers, both grape and non-grape, have no practical solutions for protecting their crops. The solution currently is using passive mitigation, through the use of buffer zones or avoiding planting near areas of row crop production, with either is not always being feasible. Grapes are especially sensitive to AHLH, with visible injury occurring at even 1/100th of the labeled application rate. In Texas over 40% of growers in the High Plains and West Texas have reported injury in vines from ALH. As grape production continues to increase in Texas there is a need for a viable and cost-effective solution for the prevention of ALH damage. This study is investigating the novel use of a class of small molecules, a single candidate used in this preliminary experiment, that share a similar mode of action. These molecules compete for the binding site of the TIR1/AFB protein found in plants, to inhibit the binding of ALH. If successful, these small molecules could prevent the auxin-signaling pathway from being activated by ALH. This compound was tested in-vitro conditions using rates well above drift incidents. If effective these molecules represent the first steps in developing active mitigation strategies for the prevention of off target ALH incidents. While this study is being conducted with grapevines, should it show promise, tests could be expanded to other horticultural crops in preventing ALH damage.
Speakers Co-authors
Friday August 1, 2025 1:00pm - 1:45pm CDT
Empire AB
 


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