Muscadine grape seeds are a rich source of polyphenols with known health benefits, yet the specific compounds responsible for their anticancer effects remain poorly understood. This study investigates the cytotoxic potential of muscadine seed metabolites against African American triple-negative breast cancer using an integrated OMICS approach combining genomics, metabolomics, cytotoxicity assays, HPLC, gene expression profiling, molecular modeling, and statistical analysis. Among 1,781 metabolites identified by LC/MS-MS, we identified three compounds that exhibited the strongest anticancer activity by targeting Eph receptor signaling, cell proliferation, and inducing apoptosis. These findings highlight the therapeutic potential of muscadine seed phytochemicals and support their development as functional food components or leads for targeted cancer therapies.
Friday August 1, 2025 9:30am - 9:45am CDT Strand 11B
