Enhancing vitamin C concentration in crops like lettuce (Lactuca sativa L.) through biofortification can increase dietary intake for consumers, yet its rapid degradation post-harvest remains a challenge. This limitation necessitates practical strategies to optimize post-harvest retention of ascorbic acid and extend the shelf-life of lettuce. This study aims to assess the effect of ascorbic acid foliar sprays on post-harvest stability of vitamin C content during post-harvest storage. A shelf-life study was conducted under refrigerated (4°C) and ambient (22°C) storage conditions over a 72-hour period. Potential results suggest that higher vitamin C concentrations may enhance leaf chlorophyll content and antioxidant capacity, potentially improving overall plant health and stress resistance. However, increased vitamin C levels could also lead to greater oxidation over time, impacting nutrient retention post-harvest stability. This study is expected to demonstrate the feasibility of vitamin C biofortification as a functional food strategy, enhancing the nutritional profile of lettuce while maintaining acceptable post-harvest quality. The findings from this study could provide valuable insights into improving fresh produce shelf-life and micronutrient retention. This research hopes to address dietary deficiencies while minimizing food waste.