ASHS 2025 Conference

Kale (Brassica oleracea L. var. acephala) is a highly nutritious cruciferous vegetable valued for its abundant phytochemicals, particularly carotenoids such as β-carotene, lutein, and zeaxanthin. These compounds play crucial roles in human health by acting as antioxidants and precursors to vitamin A, aiding in the prevention of non-communicable diseases such as cancer, cardiovascular disorders, and age-related macular degeneration. Despite the health benefits of carotenoids, their concentration in kale can be significantly influenced by environmental and agronomic factors, particularly nutrient availability. Magnesium (Mg), an essential macronutrient and a core component of the chlorophyll molecule, plays a pivotal role in photosynthesis, enzyme activation, and nutrient translocation. While its role in general plant metabolism is well established, the impact of magnesium fertilization on carotenoid accumulation in kale remains underexplored. This study investigates the effect of different magnesium fertilization rates (12.3 mg/L, 24.6 mg/L, 36.9 mg/L, and 49.2 mg/L) on the accumulation of carotenoids and elemental nutrients in three kale cultivars—Darkibor, Mamba, and Red Russian—under hydroponic conditions. A split-plot experimental design will be implemented in a greenhouse using an NFT (Nutrient Film Technique) system. The research will evaluate chlorophyll (‘a’ and ‘b’) contents in the leaves, fresh and dry biomass, crop height, crown diameters, elemental nutrient concentrations using the ICP mass spectroscopy method, and carotenoid levels through HPLC analysis. The results will be statistically analyzed using ANOVA in RStudio. This study seeks to identify optimal magnesium application rates that enhance the nutritional quality of kale, providing insights into sustainable fertilization strategies for maximizing the health benefits of this functional crop.