Zoysiagrass is a popular choice for Florida lawns but requires supplemental fertilization and irrigation. Nitrogen-fixing bacteria, such as Azospirillum Brasilense (Azb), may improve nutrient uptake and rooting, potentially reducing fertilizer and irrigation needs. The experiment is taking place from Summer 2024 to Fall 2026 at the University of Florida, evaluates the effects of Azb on Zoysiagrass under varying nitrogen (N) and irrigation rates. The experiment uses ‘CitraZoy’ Zoysiagrass treated with three Azb products (Tazo-B, AzoPro Turf, Azo Root) and a non-inoculated control. Treatments are applied monthly at three N levels (100%, 50%, 0%) and two irrigation levels (100% and 75% recommended irrigation), using a randomized complete block design with four replicates per treatment. Weekly data collection includes normalized difference vegetation index (NDVI), visual quality, soil moisture, and digital image analysis. Statistical analysis is performed using ANOVA, with mean comparisons determined by Fisher’s Protected Least Significant Difference (LSD) at p ≤ 0.05. Results indicate that Azb products mitigate the impact of reduced irrigation on soil moisture. Combining Tazo-B or Azo Root with 50% N achieves an optimal balance of turf quality and sustainability. Additionally, Tazo-B with 100% N and 75% irrigation yields green cover comparable to fully irrigated treatments. These first-year findings suggest Azb enhances Zoysiagrass performance while reducing nitrogen and irrigation requirements. A second year of research will confirm these results, supporting the potential of Azb to improve turfgrass sustainability.
