Macadamia integrifolia and M. tetraphylla, unlike M. ternifolia, are known for their edible nuts. All three species over-accumulate the trace metal nutrient manganese (Mn) in their shoots. This study seeks to examine tissue- and cellular-level distribution of Mn and other plant nutrients in the three Macadamia species. The distribution of Mn, calcium (Ca), iron (Fe) and potassium (K) were investigated in whole leaves and cross-sections of roots, petioles and lea ves using synchrotron-based X-ray Fluorescence Microscopy (µXRF) in M. integrifolia, M. tetraphylla and M. ternifolia. The results show Mn sequestration primarily in the leaf and midrib palisade mesophyll cells of all three species. Leaf interveinal regions, root cortical cells and phloem cells were also found to be Mn-loaded. The current study confirms earlier findings but further reveals that Mn is concentrated in the vacuoles of mesophyll cells owing to the exceptional resolution of the synchrotron µXRF data, and the fact that fresh hydrated samples were used. New insights gained here into Mn compartmentalisation in these highly Mn-tolerant Mac adamias expand knowledge about potentially toxic overaccumulation of an essential micronutrient, which ultimately stands to inform strategies around farming edible species in particular.
