Vitamin C is a vital antioxidant that plays a crucial role in plant photosynthesis, enzymatic reactions, and stress resistance, while also being a key micronutrient for human health. Evaluating the potential of enhancing vitamin C content in food crops like lettuce (Lactuca sativa L.) can contribute to better health outcomes and disease prevention. This study investigates the effect of ascorbic acid foliar application on biomass, phytochemicals, and mineral nutrient content in lettuce using a Nutrient Film Technique (NFT) hydroponic system. Lettuce was treated with different concentrations of ascorbic acid (0, 500, 750, and 1000 ppm) via foliar application. Chlorophyll and biomass were recorded, while vitamin C content was analyzed using high-performance liquid chromatography (HPLC). Other micronutrients were also analyzed and assessed based on treatments. Results from this study will contribute to understanding how micronutrient deficiency in humans can be addressed and potentially maximized through agronomic biofortification.
