Nutrient biofortification through micronutrient supplementation offers a practical approach to enhancing the nutritional profile of leafy vegetables while promoting sustainable food production. Selenium (Se), a vital trace element for human health, plays a significant role in plant metabolism and the synthesis of antioxidant compounds such as beta-carotene. However, just a few research has explored selenium's effect on beta-carotene accumulation in upland cress (Barbarea verna) under controlled greenhouse conditions. This ongoing study evaluates the effect of selenium biofortification on beta-carotene accumulation in upland cress using a deep-water hydroponic system. The experiment is laid out in a randomized complete design with a control and three selenium treatment levels (0, 1.0, 2.0, and 4.0 mg Se·L⁻¹), corresponding to control, low, medium, and high concentrations. Sodium selenate serves as the selenium source. Key growth parameters and environmental conditions such as pH and electrical conductivity (EC) of the nutrient solution are monitored throughout the cultivation period. Half Strength Hoagland solution is being used as a nutrient solution for this research. Plant samples will be analyzed to determine the relationship between selenium uptake and beta-carotene content using spectrophotometric analysis. This research aims to identify the optimal selenium concentration that enhances beta-carotene synthesis without compromising plant growth or yield. Preliminary observations suggest a dose-dependent physiological response to selenium supplementation, which may contribute to biofortification strategies for functional leafy greens. Findings from this study will inform controlled environment agriculture practices and support the development of nutrient-enriched crops for improved human health. Keywords: Selenium biofortification, Beta-carotene, Upland cress, Hydroponics, Controlled environment, Greenhouse production, Functional foods, Nutritional enhancement.
