Previous research demonstrated that tissue culture (TC) ginger grown in a high tunnel (HT) produced a higher yield than traditionally seed-sprouted ginger. This study further investigated any significant changes between generations of TC ginger in a HT. We studied 6 cultivars (BB, BG, BK, HY, KM, and MD) from two TC generations (TCF1 and TCF2). TCF1 seedlings were initially acclimated in a greenhouse and then transplanted into HT with TCF2 seed in June 2024. Randomized complete block design (RCBD) was used with 3 replications (blocks) per TC generation, 12 plots per block, 8 plants per plot (5 plants for HY) for a total number of 576 plants. Data collected: stem number