Parrotia subeaqualis, a recently introduced plant species in the US, exhibits limited genetic diversity due to the limited collection area. Mirroring the declining populations in their native China, limited genetic sampling can impair cultivar development and threaten cultivars' long-term survival. Conservation efforts and providing the initial plant material for breeding programs rely on efficient and dependable propagation methods. This research focuses on developing optimized in vitro establishment and regeneration protocols for various genotypes of Parrotia spp. We conducted experiments to identify the most effective treatment options and media compositions for in vitro initiation and plant regeneration. These experiments explored using different tissue types collected throughout the growing season, basal media, plant growth regulators (PGRs), and other additives. Initial trials with P. subaequalis were unsuccessful due to heavy contamination. Systematically testing different combinations, we determined the specific stimuli required to induce regeneration-competent tissue for diverse Parrotia lineages. Subsequent experiments on P. subaequalis revealed that the most effective media for P. subaequalis was Murashige
